Temperature, mosquito feeding status and mosquito density influence the measured bio-efficacy of insecticide-treated nets in cone assays

Table 1 Effect of exposure temperature on Anopheles funestus mortality measured on cone bioassays

Temperature (˚C)	n	24 h mortality		48 h mortality		72 h mortality
Temperature (˚C)	n	OR^a (95% CI)	P value	OR^a (95% CI)	P value	OR^a (95% CI)	P value
MAGNet
26–27	60	Reference		Reference		Reference
22–23	60	0.83 (0.39–1.78)	0.636	1.10 (0.50–2.42)	0.812	0.55 (0.27–1.09)	0.085
29–30	60	2.93 (1.59–5.39)	0.001	2.49 (1.32–4.67)	0.005	2.08 (1.23–3.52)	0.007
32–33	60	3.96 (1.99–7.87)	< 0.001	3.27 (1.44–7.45)	0.005	2.22 (1.10–4.49)	0.027
Veeralin
26–27	60	Reference		Reference		Reference
22–23	60	0.47 (0.31–0.73)	0.001	0.29 (0.17–0.51)	< 0.001	0.46 (0.27–0.80)	0.005
29–30	60	12.59 (7.51–21.10)	< 0.001	16.83 (8.34–33.97)	< 0.001	21.25 (10.18–44.38)	< 0.001
32–33	60	22.20 (11.45–43.05)	< 0.001	28.20 (12.10–65.72)	< 0.001	36.81 (15.35–88.23)	< 0.001

CI Confidence interval, ITN insecticide-treated net, n number of replicates, OR odds ratio
^aThe ORs were derived from mixed-effects logistic regression adjusted for net type, cone replicate, experimental time and day of the experiment, with observation was included as a random effect

ISSN: 1756-3305