Temperature, mosquito feeding status and mosquito density influence the measured bio-efficacy of insecticide-treated nets in cone assays

Table 3 Effect of mosquito density on An. funestus mortality measured on cone bioassays

Mosquito density	n	24 h mortality		48 h mortality		72 h mortality
Mosquito density	n	OR^a (95% CI)	P value	OR^a (95% CI)	P value	OR^a (95% CI)	P value
MAGNet
5	60	Reference		Reference		Reference
10	60	1.81 (1.03–3.20)	0.040	1.14 (0.72–1.80)	0.578	0.83 (0.57–1.21)	0.334
15	60	2.16 (1.25–3.71)	0.006	1.07 (0.69–1.65)	0.778	0.68 (0.48–0.98)	0.037
20	60	2.74 (1.61–4.67)	< 0.001	1.44 (0.94–2.21)	0.090	0.90 (0.64–1.26)	0.539
Veeralin
5	60	Reference		Reference		Reference
10	60	2.06 (1.24–3.42)	0.005	2.03 (1.16–3.57)	0.013	1.94 (1.11–3.39)	0.019
15	60	2.08 (1.27–3.42)	0.004	2.15 (1.23–3.73)	0.007	1.91 (1.11–3.28)	0.020
20	60	5.05 (3.03–8.43)	< 0.001	4.89 (2.78–8.61)	< 0.001	4.10 (2.35–7.15)	< 0.001

CI Confidence interval, ITN insecticide-treated net, n number of replicates, OR odds ratio
^aThe ORs were derived from mixed-effects logistic regression adjusted for net type, cone replicate, experimental time and day of the experiment, with observation was included as a random effect

ISSN: 1756-3305