Table 5 Detection of Anaplasma marginale, Babesia bigemina and Babesia bovis by means of conventional PCR (DNA), serology (iELISA) and blood smears using blood samples collected from cattle subjected to different control schemes against Rhipicephalus microplus
Tick fever agent | Animal age and treatment | |||||||
|---|---|---|---|---|---|---|---|---|
4Â months | 6Â months | 8Â months | 10Â months | |||||
Treatment (FLU) | Control (FIFLUA ) | Treatment (FLU) | Control (FIFLUA) | Treatment (FLU) | Control (FIFLUA) | Treatment (FLU) | Control (FIFLUA) | |
PCR | ||||||||
 B. bovis | 0/15 (0) | 0/15 (0) | 2/15 (13.3) | 3/15 (20) | 0/15 (0) | 0/15 (0) | 0/15 (0) | 1/15 (6.6) |
 B. bigemina | 5/15 (33.3) | 11/15 (73.3) | 9/15 (60) | 12/15 (80) | 12/15 (80) | 15/15 (100) | 13/15 (86.7) | 15/15 (100) |
 A. marginale | 14/15 (93.3) | 13/15 (86.6) | 15/15 (100) | 15/15 (100) | 15/15 (100) | 15/15 (100) | 15/15 (100) | 15/15 (100) |
iELISA | ||||||||
 B. bovis | 3/15 (20) | 1/15 (6.6) | 5/15 (33.3) | 5/15 (33.3) | 4/15 (26.6) | 7/15 (46.6) | 6/15 (40) | 7/15 (46.6) |
 B. bigemina | 11/15 (73.3) | 11/15 (73.3) | 14/15 (93.3) | 15/15 (100) | 14/15 (93.3) | 13/15 (86.6) | 15/15 (100) | 15/15 (100) |
 A. marginale | 4/15 (26.6) | 7/15 (46.6) | 7/15 (46.6) | 8/15 (53.3) | 14/15 (93.3) | 14/15 (93.3) | 15/15 (100) | 15/15 (100) |
Blood smear* | ||||||||
 B. bovis | 0/30 (0) | 0/30 (0) | 2/30 (6.6) | 0/30 (0) | 10/30 (33.3) | 7/30 (23.3) | 1/30 (3.3) | 0/30 (0) |
 B. bigemina | 01/30 (3.3) | 0/30 (0) | 1/30 (3.3) | 1/30 (3.3) | 14/30 (46.6) | 11/30 (36.6) | 3/30 (10) | 0/30 (0) |
 A. marginale | 19/30 (63.3) | 17/30 (56.6) | 27/30 (90) | 30/30 (100) | 30/30 (100) | 30/30 (100) | 30/30 (100) | 30/30 (100) |