Table 2 Cercariae trematode identification results based on the investigation of the nuclear (18S rDNA and ITS region) and mitochondrial (COI) DNA sequences
Trematode sample | 18S rDNA | ITS region | COI mtDNA | Species identification conclusion |
|---|---|---|---|---|
V_RAD_1 | 1043 bp—100% (AJ287547)—Notocotylus sp. | 1240 bp—97.7% (JQ766940)—Notocotylus malhamensis | 419 bp—99.5% (MH369327)—Notocotylus sp. | Notocotylus sp. (Fig. 4B) |
V_RAD_3 | 1050 bp—100% (OP627676)—Echinostoma revolutum | 950 bp—100% (OP627676)—Echinostoma revolutum / 100% (AY168931)—Echinoparyphium recurvatum | 890 bp—87.8% (MH369225)—Echinoparyphium sp. (82% (OR030109)—Echinostoma revolutum) | Echinostomatidae sp. (Fig. 4C) |
V_RAD_4 | 1040 bp—100% (AY157218)—Trichobilharzia regenti | 1070 bp—99.9% (OX235267)—Trichobilharzia regenti | 800 bp—100% (HM439500)—Trichobilharzia regenti | Trichobilharzia regenti (Fig. 4A) |