Table 2 Primers of the six microsatellite loci in Oncomelania robertsoni
Locus | Primer sequence (5ʹ → 3ʹ) | Repeat motif | Fluorescent labeling |
|---|---|---|---|
P82 Pf | AAGAACTGCTCATACTGGAAAG | (GGA)4(GAA)12 | FAM |
P82 Pr | GTGGTGCCCCTACGACCT | ||
P84 Pf | GTTGCAGATTCCGAAAGA | (TACAT)3 | HEX |
P84 Pr | GATCAGGGGTTGTCCAGT | ||
T4-22 Pf | TATCCAAGAAGCCGAAAC | (CA)10 | TAMRA |
T4-22 Pr | GAGGAAAGCGAGGTAAGA | ||
T5-11 Pf | ACGCCAGTCTTGGTGTCA | (GT)14 | FAM |
T5-11 Pr | TACTTGGGCAGAAGGGTT | ||
T5-13 Pf | TAGTGGGACTTATTTGCTG | (GT)14…(GT)6 | HEX |
T5-13 Pr | AAGGCTGAGTGGTAGTTA | ||
T6-27 Pf | AATGACACCCCGAACAAA | (G)13(GT)6…(GT)2 | TAMRA |
T6-27 Pr | CACTTCTCAACTCCAACCT |