Fig. 3
Both CFAV and ZIKV infect mosquito ovaries following IT injection. For pre-blood meal ovaries, mosquitoes were dissected 6 days post-IT injection, ovaries were fixed with 10% formalin, and the presence of the virus was visualized by ISH, using virus-specific probes. For post-blood meal ovaries, mosquitoes were dissected 5 days post-blood meal, and the presence of the virus was visualized by ISH. A Control uninfected ovaries; B and C CFAV-infected ovaries before and after blood meal, respectively; D and E ZIKV-infected ovaries before and after blood meal, respectively. Images were taken at 10× magnification