Cell fusing agent virus rarely transmits vertically in artificially infected laboratory-colonized Aedes aegypti mosquitoes

Table 1 Vertical transmission of Zika virus (ZIKV) and cell fusing agent virus (CFAV)

Virus	Experiment no.*	Gonotrophic cycle	Number of mosquitoes	Number of pools	Number of larvae/pool	Number of pools infected (C_T value)	MFIR^†
ZIKV	1	1	30	57	15	1 (22.2)	1:985
CFAV	1	1	48	107	15	0	–
	2	1	82	82**		0	–
		2	36	82	20	1 (27.4)	1:1997
	3	1	47	116	18	0	–
		2	45	86	18	1 (24.6)	1:1938

*Experiments 1 and 2 were performed with Ae. aegypti (MX) population and experiment 3 with Ae. aegypti (FL) population. 100% infection in all experiments.
**Only 20% of each mosquito progeny tested
^†The minimum filial infection rate (MFIR) was calculated as the number of positive pools:total number of larvae tested (larvae in pools + the initial 20% larvae tested separately)

ISSN: 1756-3305