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Fig. 2 | Parasites & Vectors

Fig. 2

From: Identification of host protein ENO1 (alpha-enolase) interacting with Cryptosporidium parvum sporozoite surface protein, Cpgp40

Fig. 2

Confirmation of the interactions between Cpgp40 and host protein ENO1. a Verification of recombinant protein expression in HEK293T cells. b Interactions between ENO1 and Cpgp40 in vivo as determined by coimmunoprecipitation. c Bimolecular fluorescence complementation (BiFC) assessing the interactions between ENO1 and Cpgp40 in live mammalian cells. A: positive control: pbJun-VN155 + pbFos-vc155. B: pBiFC-VC155-GP40 + pBiFC-VN155-ENO1. C: negative control: pbJun-VN155 + pbFos-vc155(delta ZIP). D: blank. (scale bar: 100 μm). The red arrow points to the fluorescent signal

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Parasites & Vectors

ISSN: 1756-3305

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