Fig. 5

Alignment of amino acid sequence and phylogenetic analysis of human ENO1 with other enolase sequences. a Alignment of amino acid sequence of human ENO1 and other species. The Mg2 + binding motifs are shown in a blue box, substrate-binding motifs are shown in a green box, plasminogen binding motifs are shown in a black box, and the enolase signature is shown in red box. The shade of the blue background represents the level of similarity. b Phylogenetic analysis of human ENO1 with other enolase sequences. The phylogenetic tree was constructed using the neighbor-joining method. The percentages of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown next to the branches