Fig. 2

ESP-induced migration (non-coated) and invasion (Matrigel-coated) of H69 and LX2 cells co-cultured in a Transwell cell culture insert. A Schematic of co-cultured H69 and LX2 cells in a Transwell insert. Cells seeded on a Transwell cell culture insert were cultured for 24 h, following which medium supplemented with 1.6 μg/ml ESPs was added to the lower chamber and cells further incubated for 1 and 3 days. B, D Cell migration (B) and invasion (D) abilities were quantified by counting crystal violet-stained cells outside the porous membranes. Scale bar: 100 μm; original magnification: ×200. C, E Data in B and D are represented in C and E, respectively, as the number of stained cells in three random fields using ImageJ software. Values are presented as the mean ± SE. Asterisks indicate significant a difference at *P < 0.05, **P < 0.01 and ***P < 0.001, compared with the untreated control. Con, Untreated control; ESPs, excretory-secretory products; SE, standard error