Fig. 3

Effects of LX2-ESPs-CM on the secretion of inflammatory cytokines and the expression of EMT-, and fibrosis-related proteins in H69 cells. H69 cells were incubated for 24 h in H69 media as a control or in the media in which LX2 cells were grown in the presence of ESPs (LX2-ESPs-CM) for 24 h, as described in the Methods section. A, B The effect of LX2-ESPs-CM on TGF-β1 and IL-6 secretion levels of H69 cells, measured using an enzyme-linked immunosorbent assay. Data are presented as the means ± SE of 3 independent experiments. Asterisks indicate a significant difference at *P < 0.05 and **P < 0.01, compared with the untreated control; the triple hash signs indicate a significant difference at ^###P < 0.001 for the control group vs the LX2 ESPs-CM group. C Expression of TGF-β1-, IL-6-, EMT- and fibrosis-related proteins. Individual bands were quantified and normalized to GAPDH. Data are presented as the mean ± SE of 3 independent experiments. Asterisks indicate a significant difference at *P < 0.05, **P < 0.01, ***P < 0.001, compared with untreated H69 cells. Con, Non-CM control; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; EMT, epithelial-mesenchymal transition; ESPs, excretory-secretory products; IL, interleukin; SE, standard error; TGF-β1, transforming growth factor beta 1