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Fig. 4 | Parasites & Vectors

Fig. 4

From: The crosstalk between cholangiocytes and hepatic stellate cells promotes the progression of epithelial-mesenchymal transition and periductal fibrosis during Clonorchis sinensis infection

Fig. 4

Immunostaining images of N-cad and fibronectin in H69 cells cultivated in media in which LX2 cells were grown in the presence of ESPs (LX2-ESPs-CM). A, C Representative immunofluorescence images of N-cad and fibronectin (green). DAPI was used as the nuclear counterstain (blue). Scale bar: 100 μm; original magnification: ×40. B, D Quantification of fluorescence intensity shown in A and C, respectively, using ImageJ software. Data in graphs are presented as the mean ± SE (n = 3). Asterisks indicate a significant difference at *P < 0.05, **P < 0.01 and ***P < 0.001, compared with untreated H69 cells. DAPI, 4′,6-Diamidino-2-phenylindole; ESPs, Excretory-secretory products; N-cad, N-cadherin; SE, standard error

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Parasites & Vectors

ISSN: 1756-3305

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