Fig. 6

Fibronectin expression in three-dimensional-cultured cells in response to ESPs. A Immunofluorescence images of fibronectin expression in H69 and LX2 cells stacked on COL1 ECM. Green indicates fibronectin and blue indicates DAPI. Merged images represent the overlap of the fibronectin and DAPI. Scale bars: 100 μm; original magnification: ×40. B Quantification of corrected total cell fluorescence. Data represent the mean ± SE. Double asterisks indicate a significant difference at **P < 0.01, for Con vs H69 Direct; double hash signs indicate a significant difference at ^##P < 0.01, for Con vs LX2 Direct; triple dollar signs indicate a significant difference at ^$$$P < 0.001, for H69 Direct vs LX2 Direct. Con, Untreated control; DAPI, 4′,6-diamidino-2-phenylindole; ESPs, excretory-secretory products; H69 Direct, ESPs applied to a H69 channel; LX2 Direct, ESPs applied to a LX2 channel; SE, standard error