Fig. 2

miR-374b-5p was directly bound to the 3ʹ-UTR of C/EBP β and suppressed its expression. A Putative binding site of miR-374b-5p in the 3ʹ-UTR of C/EBP β. B Relative luciferase activity in HEK293T cells co-transfected with miR-374b-5p mimics and pmirGLO-C/EBP β-WT or pmirGLO-C/EBP β-Mut, respectively. C Expression level of miR-374b-5p and (D) C/EBP β in KCs transfected with miR-374b-5p mimics, miR-374b-5p inhibitor or their NC. E Western blot analysis of C/EBP β expression in KCs transfected with miR-374b-5p mimics, miR-374b-5p inhibitor or their NC. F Expression level of C/EBP β in the KCs from Echinococcus multilocularis-infected mice. Corresponding p value was calculated between experimental group and control group by t-test, *p < 0.05,**p < 0.01, ***p < 0.001. C/EBP β-WT: pmirGLO-C/EBP β-Wildtype, C/EBP β-Mut: pmirGLO-C/EBP β-Mutant, 2 M-KC-C: KCs from mice treated with PBS for 2 months; 2 M-KC-E, KCs from mice 2 months post-infection with E. multilocularis