Fig. 2

Cell cycle arrest in T. gondii-infected cells is independent of the mitosis checkpoint protein control cyclin B1. Three donors of HUVEC, HFF, FHs74, BSIEC and BCEC were infected with T. gondii tachyzoites and fixed 24 h p.i. Cells were stained against cyclin B1 and an isotype control and analysed using FACS. Gating was done first by choosing the cell population and then selecting the same number of cells in each sample (A). The cyclin B1 signal was analysed as the mean of fluorescence (MOF) and plotted for non-infected and T. gondii-infected cells. The results showed that cyclin B1 was not affected after infection in any of the cell types studied (B, C). Graph bars represent the median ± SD of three biological replicates