Table 2 Primary and secondary antibodies used in the western blot (WB) and immunofluorescence assays
Antigen | Company | Cat. number | Origin/reactivity | Dilution |
|---|---|---|---|---|
Primary antibodies immunofluorescence assay | ||||
Β-catenin | Abcam | Ab32572 | Rabbit | 1:200 |
Toxoplasma gondii | Thermo Fisher | PA1-7256 | Goat | 1:100 |
Primary antibodies FACS | ||||
Cyclin B1-Alexa Fluor 647 | Cell Signaling Technology | ab32053 | Rabbit | 1:50 |
Secondary antibodies | ||||
|---|---|---|---|---|
Antigen/conjugate | Company | Cat. number | Host/target | Dilution |
Goat anti-mouse IgG peroxidase conjugated | ||||
Alexa Fluor 488 | Thermo Fisher | A11001 | Goat/mouse | 1:500 |
Alexa Fluor 594 | Thermo Fisher | A21468 | Chicken/rabbit | 1:500 |
Alexa Fluor 647 | Thermo Fisher | A21235 | Goat/mouse | 1:500 |