Table 2 Evaluation of physical and chemical properties of the designed structure using the EXPASY ProtParam Server
The physiochemical properties | Analyze |
|---|---|
Number of amino acids | 780 |
Molecular weight | 85,190.25 |
Theoretical PI | 5.98 |
Total number of negatively charged residues (Asp + Glu) | 112 |
Total number of positively charged residues (Arg + Lys) | 100 |
Estimated half-life | 20 h (mammalian reticulocytes, in vitro), 30 min (yeast, in vivo), > 10 h (E. coli, in vivo) |
Instability index | 35.80 |
Aliphatic index | 86.04 |
Grand average of hydropathicity (GRAVY) | −0.278 |