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The development of drugs for treatment of sleeping sickness: a historical review
Parasites & Vectors volume 3, Article number: 15 (2010)
Only four drugs are available for the chemotherapy of human African trypanosomiasis or sleeping sickness; Suramin, pentamidine, melarsoprol and eflornithine. The history of the development of these drugs is well known and documented. suramin, pentamidine and melarsoprol were developed in the first half of the last century by the then recently established methods of medicinal chemistry. Eflornithine, originally developed in the 1970s as an anti-cancer drug, became a treatment of sleeping sickness largely by accident. This review summarises the developmental processes which led to these chemotherapies from the discovery of the first bioactive lead compounds to the identification of the final drugs.
Human African trypanosomiasis or sleeping sickness is a disease caused by two subspecies of Trypanosoma brucei, T. b. rhodesiense and T. b. gambiense. The parasites live and multiply extracellularly in blood and tissue fluids of their human host and are transmitted by the bite of infected tsetse flies (Glossina spp.). The occurrence of sleeping sickness is restricted to the distribution of tsetse flies which are exclusively found in sub-Saharan Africa between 14°N and 20°S . More than 250 discrete active sleeping sickness foci in 36 African countries are recognised most of which are in rural areas .
Trypanosoma b. rhodesiense is found in East and southern Africa whereas T. b. gambiense occurs in West and Central Africa. The course of sleeping sickness is different depending on the subspecies. Infections with T. b. rhodesiense lead to an acute form of the disease while infections with T. b. gambiense give rise to a chronic infection. The symptoms of the first stage of the disease, defined by the restriction of trypanosomes to the blood and lymph system, include fever, headache, joint pain and itching [3, 4]. The clinical signs of the second stage of the disease, characterised by the invasion of trypanosomes into the central nervous system, are neurological and endocrinal disorders [3, 4]. If left untreated, sleeping sickness patients infected with T. b. rhodesiense will die within months whereas those infected with T. b. gambiense usually survive for several years.
In the late 19th Century, Africa experienced several sleeping sickness epidemics the most devastating of which was an epidemic with 300,000 to 500,000 deaths between 1896 and 1906 which mainly affected the Congo Basin and the Busoga focus in Uganda and Kenya . The disastrous effect of this epidemic persuaded the various colonial administrations to call for their medical scientists to develop a cure for sleeping sickness. At that time, the field of chemotherapy was developing and had begun to make use of the novel methods of medicinal chemistry, i.e. the identification, synthesis and development of new chemical entities suitable for therapeutic use. In fact, it was for the development of early anti-sleeping sickness drugs that medicinal chemistry was first used [6, 7].
The synthetic dyestuff industry evolved in the middle of the 19th Century, primarily in Germany which became the world leader . The most influential protagonist of the synthetic dyestuff industry was the German scientist Paul Ehrlich who was the first to exploit the properties of dyes for the development of chemotherapies. In 1901, Ehrlich became interested in the chemotherapy of trypanosomiasis and tested more than 100 synthetic dyes against Trypanosoma equinum, a species that causes a disease known as Mal de Caderas in equids, and T. brucei brucei, which is responsible for Nagana, a disease of cattle . The only dye displaying trypanocidal activity was a benzopurpurine compound named Nagana Red (Fig. 1). When trypanosome-infected mice were treated with Nagana Red, parasites became undetectable in the animals for a short period and the treated mice survived 2 days longer than control mice (5/6 days versus 3/4 days). Ehrlich thought that the poor efficacy of Nagana Red was due to its low solubility which impaired the absorption of the drug into the bloodstream from the subcutaneous inoculation site. In 1903, Ludwig Benda working for Cassella Farbwerke near Frankfurt synthesised a derivative of Nagana Red, called Trypan Red (Fig. 1), with an extra sulphonic acid function and enhanced water solubility . Trypan Red proved to be both curative and prophylactic for T. equinum infections in mice  but not for infections with other trypanosome species . Ehrlich investigated another 50 derivatives of Trypan Red and the 7-amino derivative of the compound was tested by the German physician Robert Koch during an expedition in 1906 . However, the 7-amino derivative was not more effective than Trypan Red itself.
From 1906, the German Bayer pharmaceutical company supplied the French bacteriologist Maurice Nicolle and the French zoologist Felix Mesnil from the Pasteur Institute with benzopurpurine dyes to be tested for trypanocidal activities . One blue benzopurpurine derivative, Trypan Blue (Fig. 1), was found to be very effective in eliminating all trypanosomes from the blood of infected animals [13, 14] but as the drug stained the skin of the animals bluish it was unacceptable for use in patients [9, 10]. For this reason Wilhelm Roehl a former assistant of Ehrlich who had joined the Bayer research group at Elberfeld in 1905, sought a colourless compounds with trypanocidal activities. The chemists Oskar Dressel and Richard Kothe of Roehl's team synthesised derivatives of Afridol Violet (Fig. 1), a naphthalene urea compound that was less colour-intensive but also less trypanocidal in screens carried out by Nicolle and Mesnil. Several derivatives, however, displayed better activity against trypanosomes than the parent compound. In 1917, after the synthesis and screening of more than 1000 naphthalene ureas, the breakthrough came in the form of Bayer 205 (Fig. 1), later named Germanin, a colourless compound that cured trypanosomiasis in both experimental animals and in humans . The Bayer Company understood the political importance of Bayer 205 for the commercial exploitation of African colonies and offered the formula of the drug to the British Government in exchange for the return of Germany's lost African territories . When the British declined the offer, the Bayer Company refused to disclose the chemical structure of the drug. Eventually, in 1924, the French pharmacist Ernest Fourneau published the structure of Bayer 205 . Four years later, the Bayer Company confirmed that Fourneau's structure was identical with that of Germanin. The drug was later renamed suramin and is still in use in the therapy of early-stage T. b. rhodesiense sleeping sickness.
In 1858, the Scottish missionary and explorer David Livingston had suggested the use of Fowler's Solution, a 1% aqueous solution of potassium arsenite, for the treatment of sleeping sickness , although the causative agent of the disease was still unknown. In 1902, the French physician Charles Louis Alphonse Laveran (who had discovered the malaria parasite in 1880) together with Mesnil reported that sodium arsenite was effective in killing trypanosomes in infected laboratory animals. Although the parasites were quickly destroyed by sodium arsenite, they reappeared in the blood of the animals within a few days and caused their death . Two years later, the Canadian doctor Harold Wolferstan Thomas published a paper describing the successful therapy of animals experimentally infected with trypanosomes with the arsenical drug Atoxyl (aminophenyl arsonic acid; Fig. 2) . The drug had already been synthesised in 1859 by the French biologist Antoine Béchamp and was claimed to be 40-50 times less toxic than arsenic acid ; hence the name Atoxyl. The original structure of Atoxyl, arsenic acid anilide, assigned by Béchamp was incorrect and it was Ehrlich and Alfred Bertheim, Ehrlich's chief organic chemist, who determined the correct structure as an amino derivative of phenyl arsenic acid  which was instrumental in the generation of new analogues (see below). During a trial to evaluate Atoxyl on sleeping sickness patients in East Africa, Robert Koch discovered that the compound was by no means nontoxic: about 2% of treated patients went blind through atrophy of the optic nerve . Based on this finding, Koch asked Ehrlich to synthesise derivatives of Atoxyl with reduced toxicity and improved efficacy. Bertheim synthesised a series of N-substituted Atoxyl derivatives. One such compound, acetylatoxyl or arsacetin (Fig. 2), was less toxic to mice, but when given in the high doses needed for therapy, the animals began to move uncontrollably in circles indicating that the vestibular nerve was damaged . This observation suggested that arsacetin might also cause blindness. In addition, Ehrlich found that arsacetin was ineffective in vitro and therefore assumed that the compound was metabolised into an active form, probably by a chemical reduction. To test his hypothesis, he asked Bertheim to synthesise the two possible types of reduction products from Atoxyl derivatives: arsenoxides and arsenobenzenes. Whereas the arsenoxides were toxic to both trypanosomes and the host, arsenobenzenes were less trypanocidal but still more potent than arsenoxides and less cytotoxic [6, 9]. This meant that arsenobenzenes could be administrated at sufficiently low doses to avoid the neurotoxicity problems. The most promising arsenobenzene turned out to be arsenophenylglycine (Fig. 2). When tested in humans in 1907 it proved to be safe and effective, except for a few patients who developed a severe and often fatal hypersensitivity reaction [6, 9].
Ehrlich obtained another arsenobenzene, arsenophenol, by inserting a hydroxyl group in para position of the benzene ring of Atoxyl followed by reduction. This compound was highly effective against trypanosomes but prone to oxidation and difficult to purify. Based on his experience with chemotherapeutic dyes, Ehrlich knew that the addition of a substituent in ortho position to the hydroxyl group would enhance the chemotherapeutic activity. The introduction of an amino group led to the compound 606 or arsphenamine, which was synthesised by Bertheim in 1907 but which turned out to be ineffective against trypanosomes [6, 9]. Interestingly, however, in 1909 Ehrlich and his colleague Sahachiro Hata, a Japanese bacteriologist, discovered that arsphenamine had excellent curative properties against syphilis. It became the first truly effective drug for treatment of the disease and was marketed by Farbwerke Hoechst under the proprietary name Salvarsan, 'the arsenic that saves' .
In 1919, the American chemist Walter Jacobs and the American immunologist Michael Heidelberger reported the synthesis of tryparsamide (Fig. 2), a derivative of Atoxyl . As this compound was able to enter the central nervous system it was the first drug for treating the second stage of sleeping sickness alone or in combination with suramin. Although tryparsamide also caused damage to the optical nerve, it remained the drug of choice for chemotherapy of sleeping sickness until the early 1960s  and was also used in the treatment of animal trypanosomiasis .
In 1938, the Swiss pathologist, microbiologist and chemist Ernst Friedheim synthesised melarsen (Fig. 2), a melamine derivative of Atoxyl . Friedheim introduced the melamine moiety because he observed that all arsenicals with significant trypanocidal activity contain nitrogen in one form or another . Although melarsen was less toxic than existing drugs, it was more expensive. Bearing in mind that trivalent arsenicals are much more potent than pentavalent arsenicals, Friedheim synthesised the trivalent analogue of melarsen, melarsen oxide (Fig. 2). This was very effective against trypanosomes but also very toxic . Based on the observation that a diet of Swiss cheese improved the tolerability of melarsen oxide in some patients , Friedheim had the brilliant idea to combine the drug with dimercaprol, an arsenic antidote also known as British anti-Lewisite (BAL) developed during World War II to protect against poisoning by the arsenic gas, lewisite. The resulting product, melarsoprol (also known as Mel B) (Fig. 3), was 100 times less cytotoxic but only 2.5 times less trypanocidal than melarsen oxide . Melarsoprol was introduced in 1949 for the treatment of sleeping sickness. The major advantage of melarsoprol over tryparsamide is that it does not show any toxic effect on the optic nerve. However, melarsoprol causes reactive encephalopathy in 5-10% of patients, with 1-5% mortality . Melarsoprol is still the only effective drug for chemotherapy of second stage of T. b. rhodesiense sleeping sickness.
In 1929 it was shown for the first time that pathogenic trypanosomes use enormous amounts of sugar for their metabolism ; within 24 h they consume about twice their own mass of sugar. In 1935, this knowledge prompted the American bacteriologist Hildrus Poindexter to treat animals infected with Trypanosoma equiperdum, a trypanosome that causes Dourine or covering disease in horses, with insulin to reduce their blood glucose levels. He discovered that trypanosome-infected animals subjected to insulin treatment survived longer and with fewer parasites in the blood compared to controls . In the same year, von Jancsó and von Jancsó and Schern and Artagaveytia-Allende independently found that the hypoglycaemic drug synthalin (Fig. 4) had trypanocidal action in mice and rats [29, 30]. In 1937, Yorke and Lourie at the Liverpool School of Tropical Medicine discovered that the anti-trypanosomal effect of synthalin had nothing to do with lowering the blood glucose level in the infected animals but that synthalin itself was trypanocidal . When Harold King at the National Institute of Medical Research in London learnt about the trypanocidal action of synthalin, he synthesised and tested related compounds and found that diamidino-1,11-n-undecane (Fig. 4) was particularly active against T. b. rhodeseinse in mice . Meanwhile, the English chemist Arthur James Ewins of the pharmaceutical company May and Baker prepared a large number of aromatic diamidines in which the polar amidine groups were separated by two phenyl groups rather than by a polymethylene group. Many of these compounds displayed trypanocidal activity, especially stilbamidine and pentamidine (Fig. 4) [33, 34]. Both compounds were also highly effective against human trypanosomiasis . Whereas stilbamidine was later abandoned, because it caused serious neurological effects in some patients [36, 37], pentamidine is still used for treatment of the first stage of T. b. gambiense sleeping sickness. Pentamidine is also used in the treatment of leishmaniasis and Pneumocystis jirovecii pneumonia, mostly in AIDS patients .
In 1977, Das and Boykin reported on the trypanocidal activity of a series of novel aromatic diamidines, with DB75 (2,5-bis(4-aminophenyl)-furan; Fig. 4) as the most trypanocidal compound active against T. b. rhodesiense in mice and rhesus monkeys [39, 40]. As DB75 is poorly absorbed across the gastrointestinal tract due to its positively charged amidine group, a prodrug, DB289 (2,5-bis(4-amidinophenyl)-furan-bis-O-methylamidoxime; Fig. 4), was synthesised . DB289 was the first oral drug for treatment of first-stage sleeping sickness to enter clinical trials . However, an extended phase I study to complete the safety assessment for registration of DB289 for sleeping sickness revealed severe liver toxicity and delayed renal insufficiency . As a consequence, the program to develop DB289 as an oral drug for treatment of sleeping sickness was discontinued .
Nifurtimox, a nitrofuran derivative (Fig. 5), was developed in the 1960s by the Bayer Company. Its trypanocidal activity was empirically discovered and since 1967 it has been used for the treatment of Chagas disease caused by Trypanosoma cruzi in Latin America. The mode of action of nifurtimox is not clearly known but may be related to the generation of reactive oxygen species which damage cellular components such as DNA, membrane lipids and proteins . Nifurtimox was also tried on compassionate grounds for treatment of sleeping sickness patients who were infected with melarsoprol-resistant T. b. gambiense. Small clinical trials showed that high dosage of nifurtimox was necessary to achieve a cure [44, 45]. More recently, nifurtimox has been evaluated together with eflornithine as combination therapy for treatment of late-stage T. b. gambiense sleeping sickness (see below).
Eflornithine (α-difluoromethylornithine, DFMO, Fig. 6) was initially developed in the 1970s  as a potential anti-cancer drug . Eflornithine was one of several fluorinated analogues of amino acids that were rationally designed based on a predicted enzyme reaction to generate mechanism-based inhibitors of amino acid decarboxylase. It is a specific and irreversible suicide inhibitor of ornithine decarboxylase [46, 48], the first enzyme involved in polyamine biosynthesis. Inhibition of ornithine decarboxylase results in the depletion of the polyamines putrescine and spermidine, with the consequence of a slowdown in cell proliferation. It also causes a loss of the unique antioxidant metabolite, trypanothione, which may also play a role in its selective action and also accounts for the known synergism with other drugs . The very rapid turnover rate of mammalian ornithine decarboxylase (t1/2 = 10-30 min [48, 50]), however, made DFMO ineffective as an anti-cancer drug.
In 1980, the American biologist Cyrus Bacchi heard about DFMO and tested the drug in a murine trypanosomiasis model and showed that DFMO cured mice infected with a virulent strain of T. b. brucei without any apparent toxic side effects . Based on this remarkable result, several clinical trials were carried out demonstrating that DFMO can cure second-stage T. b. gambiense sleeping sickness patients who were refractory to melarsoprol treatment [52–55]. The reason why DFMO is only active against T. b. gambiense is that the ornithine decarboxylase of this trypanosome species is very stable (t1/2 = 18-19 h) . In contrast, the DFMO tolerance of T. b. rhodesiense is due to a faster turnover rate of the enzyme (t1/2 = 4.3 h) . In 1990, DFMO was approved for the treatment of human trypanosomiasis caused by T. b. gambiense  and is currently the only treatment available for melarsoprol-refractory sleeping sickness.
In recent years, eflornithine has been tested in combination with nifurtimox in the treatment of second-stage T. b. gambiense sleeping sickness [58–60]. A recently completed multicentre, randomised, phase III trial revealed that the efficacy of nifurtimox-eflornithine combination therapy is no worse than that of eflornithine monotherapy . However, this combination therapy represents a major advance in terms of making the treatment safer, cheaper and easier to administer .
The development of the anti-sleeping sickness drugs is an early example of the employment of medicinal chemistry, the stepwise application of structure-activity relationships in order to increase the trypanocidal activity of a lead compound and to simultaneously reduce toxic side effects by modifying its chemical structure. Rational drug design, the inventive process of finding new drugs based on the knowledge of a biological target, has so far produced only one drug for treatment of sleeping sickness, eflornithine, although this drug was initially developed as an anti-cancer agent. Thus, it seems that the traditional methods of medical chemistry may be more effective in the development of new chemotherapies for this important disease.
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I thank Professor Frank Cox and Dr Kevin Tyler for critical reading of the manuscript.
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Steverding, D. The development of drugs for treatment of sleeping sickness: a historical review. Parasites Vectors 3, 15 (2010). https://doi.org/10.1186/1756-3305-3-15
- Ornithine Decarboxylase
- Human African Trypanosomiasis