- Open Access
Comparative cost assessment of the Kato-Katz and FLOTAC techniques for soil-transmitted helminth diagnosis in epidemiological surveys
© Speich et al; licensee BioMed Central Ltd. 2010
Received: 1 July 2010
Accepted: 14 August 2010
Published: 14 August 2010
The Kato-Katz technique is widely used for the diagnosis of soil-transmitted helminthiasis in epidemiological surveys and is believed to be an inexpensive method. The FLOTAC technique shows a higher sensitivity for the diagnosis of light-intensity soil-transmitted helminth infections but is reported to be more complex and expensive. We assessed the costs related to the collection, processing and microscopic examination of stool samples using the Kato-Katz and FLOTAC techniques in an epidemiological survey carried out in Zanzibar, Tanzania.
We measured the time for the collection of a single stool specimen in the field, transfer to a laboratory, preparation and microscopic examination using standard protocols for the Kato-Katz and FLOTAC techniques. Salaries of health workers, life expectancy and asset costs of materials, and infrastructure costs were determined. The average cost for a single or duplicate Kato-Katz thick smears and the FLOTAC dual or double technique were calculated.
The average time needed to collect a stool specimen and perform a single or duplicate Kato-Katz thick smears or the FLOTAC dual or double technique was 20 min and 34 sec (20:34 min), 27:21 min, 28:14 min and 36:44 min, respectively. The total costs for a single and duplicate Kato-Katz thick smears were US$ 1.73 and US$ 2.06, respectively, and for the FLOTAC double and dual technique US$ 2.35 and US$ 2.83, respectively. Salaries impacted most on the total costs of either method.
The time and cost for soil-transmitted helminth diagnosis using either the Kato-Katz or FLOTAC method in epidemiological surveys are considerable. Our results can help to guide healthcare decision makers and scientists in budget planning and funding for epidemiological surveys, anthelminthic drug efficacy trials and monitoring of control interventions.
Chronic infections with one or several of the common soil-transmitted helminths, Ascaris lumbricoides, Trichuris trichiura and the hookworms (Ancylostoma duodenale and Necator americanus), might account for a global burden of 39 million disability-adjusted life years (DALYs) lost annually [1, 2]. School-aged children in the developing world are at highest risk of morbidity due to soil-transmitted helminthiasis.
In the current era of 'preventive chemotherapy', that is the large-scale administration of anthelminthic drugs to school-aged children and other populations at risk of morbidity , diagnosis is often neglected and cost-effectiveness considerations are necessary. Yet, diagnosis is of paramount importance for an accurate assessment of the epidemiological situation and burden of disease estimations, and for monitoring drug efficacy and pharmacovigilance [4–7]. In epidemiological surveys pertaining to soil-transmitted helminthiasis (and intestinal schistosomiasis), the Kato-Katz technique  is a widely used diagnostic approach [2, 9–11]. Indeed, the method is relatively straightforward, requires minimal equipment which is mostly reusable, and hence the method is thought to be inexpensive [7, 10]. Moreover, the Kato-Katz method is simple to apply and laboratory workers can be trained within half a day . A drawback of the Kato-Katz method, however, is its lack of sensitivity for detecting light-intensity soil-transmitted helminth infections [9, 13]. New research has revealed that the recently developed FLOTAC technique  shows a higher sensitivity than multiple Kato-Katz thick smears for the diagnosis of soil-transmitted helminth infections [15–17]. Compared to the Kato-Katz method, the FLOTAC is a more complicated technique, and hence requires better equipped laboratories [18–20] and more extensive training of laboratory workers.
Here, an economic evaluation of the Kato-Katz and FLOTAC techniques was performed within the frame of an epidemiological survey. Specifically, the costs related to stool collection, transfer to the laboratory, preparation and microscopic examination of single and duplicate Kato-Katz thick smears and the FLOTAC dual or double technique were determined. This information is relevant for researchers and disease control managers for the planning of epidemiological surveys and the monitoring and evaluation of soil-transmitted helminthiasis control programmes.
Materials and methods
Study area, population and ethical considerations
This study was carried out on Unguja, the largest island belonging to the Zanzibar archipelago in Tanzania, between March and May 2009. The two primary schools of Kinyasini and Kilombero, located 26 and 32 km north-east of Zanzibar Town, were the selected field sites. Laboratory examinations were conducted in the Helminth Control Laboratory Unguja (HCLU), in Zanzibar Town. The study was readily embedded in a randomised controlled trial, assessing the efficacy and safety of four anthelminthic drug regimens against T. trichiura and other soil-transmitted helminths. At the beginning of the study, 1,066 schoolchildren aged between 6 and 20 years, were screened for soil-transmitted helminth infections (Knopp S, Mohammed KA, Speich B, Hattendorf J, Khamis IS, Khamis AN, Stothard JR, Rollinson D, Marti H, Utzinger J: "Albendazole and mebendazole administered alone or in combination with ivermectin against Trichuris trichiura: a randomized controlled trial", submitted).
The study was approved by the ethics committee of Basel, Switzerland (EKBB; reference no. 13/09) and the Ministry of Health and Social Welfare (MoHSW) of Zanzibar (reference no. ZAMEC/0001/09). Parents or legal guardians of participating children signed a written informed consent sheet. Children consented orally to participate. At the end of the study, all children attending the primary schools of Kinyasini and Kilombero were treated with single oral doses of albendazole (400 mg) and praziquantel (40 mg/kg) free of charge.
Field and laboratory procedures
The headmasters of Kinyasini and Kilombero primary schools were informed about the purpose and procedures of the study. After having obtained written informed consent by the parents/legal guardians and oral consent by the children, the field work was launched. Every morning, starting at 07:00 hours at HCLU, stool containers and collection shelves were loaded onto a 4-wheel drive (4-WD) car, and a team consisting of 4-6 workers from HCLU visited either Kinyasini or Kilombero schools. The team labeled empty containers with unique identification (ID) numbers, distributed these to the children and collected filled containers that had been distributed the day before. Each day, approximately 100 children were enrolled and lime-sized early morning stool samples were collected.
The filled containers were transferred to HCLU within 2-3 h and processed as follows. Immediately after arrival at HCLU, duplicate Kato-Katz thick smears from each stool sample were prepared by 2-8 members of HCLU, using 41.7 mg templates . After a clearing time of 20-40 min, each Kato-Katz thick smear was examined quantitatively for hookworm eggs by one of four experienced microscopists. In the afternoon, 3-6 h after slide preparation, the thick smears were re-examined by one of four additional microscopists, who counted eggs of A. lumbricoides and T. trichiura and recorded them separately.
Once a Kato-Katz thick smear had been prepared, the stool sample was placed back into the collection shelf ordered by increasing ID. A third of the stool samples was taken and a small amount of stool was weighed to ~1 g using a Kern balance (EMB basic balance; Ballingen-Frommern, Germany) and preserved in a pre-labeled tube containing 10 ml of 5% formaldehyde. The preserved stool samples were stored at room temperature and examined by the FLOTAC technique within 3 weeks after completion of the clinical trial in late May 2009 .
While technicians from HCLU were already acquainted with the Kato-Katz technique, a 3-day training course was offered for the FLOTAC technique, facilitated by two experts from the University of Naples, Italy. During the training, the FLOTAC dual technique was employed, using flotation solutions 4 (FS4; sodium nitrate, NaNO3, specific gravity (s.g.) = 1.20) and 7 (FS7; zinc sulfate, ZnSO4·7H2O, s.g. = 1.35).
Working steps and number of time measurements to determine the costs of the Kato-Katz and FLOTAC method for the diagnosis of soil-transmitted helminths in Zanzibar.
Number of measurementsa
Kato-Katz (K-K) measurements
(K1a) Labeling of K-K slides
19 × 5; 2 × 4; 1 × 6
(K1b) Preparation of duplicate K-K slides
121 × 1; 6 × 0.5; 5 × 2; 1 × 3
(K2) Microscopy morning
32 × 1; 22 × 2; 22 × 8; 21 × 4; 1 × 5
(K3) Microscopy afternoon
44 × 1; 26 × 8; 24 × 2; 24 × 4
(K6a) Washing of K-K slides
1 × 180
(K6b) Washing spatula and template
1 × 91; 1 × 96; 1 × 110
(K6c) Cutting cellophane paper
1 × 82
(K6d) Cutting wire mesh
1 × 206
(F1) Measurement of 1 g of stool and homogenization
29 × 5; 1 × 6; 1 × 7; 1 × 10
(F2a) Filter (inclusive labeling)
31 × 1
(F2b) Filter (exclusive labeling)
25 × 1; 4 × 3; 2 × 2
(F2c) Labeling Falcon tubes
3 × 6; 2 × 4; 1 × 7
(F3) Centrifugation of tubes and filling with FS
20 × 3; 1 × 2
(F4) Assembling FLOTAC apparatus
29 × 1
(F5) Filling FLOTAC apparatus
55 × 1
(F6) Centrifugation and translation of FLOTAC apparatus
18 × 2
(F7) Reading first chamber
120 × 1
(F8) Reading second chamber
119 × 1
(F11a) Washing filter and beaker
9 × 1; 9 × 6; 1 × 4; 1 × 7; 1 × 8
(F11b) Opening FLOTAC apparatus
33 × 1; 1 × 2; 1 × 3; 1 × 14
(F11c) Washing FLOTAC apparatus
2 × 3; 1 × 2; 1 × 4; 1 × 14
(F11d) Washing Falcon tube
3 × 4; 2 × 5; 1 × 101; 1 × 84; 1 × 1
(F11e) Washing pipettes
9 × 1; 1 × 105; 1 × 84; 1 × 1
Measurements for both techniques
(S1-S3) Collection of stool samples at school
(K4/F9) Data entry (demographic data)
1 × 13
(K5/F10) Data entry (results)
5 × 22; 5 × 23; 3 × 20; 3 × 9; 2 × 14; 1 × 5; 1 × 15; 1 × 21; 1 × 26
(K6f/F11f) Putting a piece of paper into an empty container to alleviate cleaning of container
7 × 10; 1 × 68
(K6g/F11g) Removing stool out of container (before cleaning)
4 × 10; 2 × 36; 1 × 38; 1 × 52; 1 × 58
(K6h/F11h) Washing of stool container (without lid)
5 × 10; 1 × 15
(K6i/F11i) Washing lid
3 × 10; 1 × 5; 1 × 6; 1 × 15
(K6j/F11j) Erasing of IDs from stool container
18 × 10; 1 × 110
Costs due to salaries
To assess the costs due to salaries, the salary of each member of the HCLU, according to his or her professional degree, was determined using a questionnaire. Additionally, the time for each working step in the field and in the laboratory was measured several times and averaged. Finally, the number of employees with a specific occupation needed to collect, process and examine 100 stool samples per day using the Kato-Katz or FLOTAC technique was calculated.
For the collection of stool samples in the field the following steps were recorded: (i) the time from departure in the laboratory, school visit and return to the laboratory; (ii) the number of personnel who visited the school; and (iii) the number of stool samples collected per day. Using these data, the average person-time to collect one stool sample was estimated.
For the FLOTAC technique the following steps were included: (F1) weighing ~1 g of stool and homogenization in 10 ml of 5% formaldehyde; (F2) filtering homogenized stool and transfer into two 15-ml Falcon tubes labeled with personal ID; (F3) centrifugation of Falcon tubes, discarding supernatant and filling FS4 into tubes; (F4) assembly of FLOTAC apparatus; (F5) filling each chamber of the FLOTAC apparatus with the homogenized stool suspension (pellet and FS4) from one of the two Falcon tubes; (F6) centrifugation and translation of FLOTAC apparatus; (F7) reading chamber 1 of FLOTAC apparatus quantitatively for A. lumbricoides, hookworm and T. trichiura eggs; (F8) reading chamber 2 of FLOTAC apparatus quantitatively for A. lumbricoides, hookworm and T. trichiura eggs; (F9) data entry (children's name, age, sex and school grade); (F10) data entry (results from all FLOTAC readings); and (F11) washing and preparing materials for the next day.
The time to prepare and read a single Kato-Katz thick smear was assessed as follows: (K1a) the time needed to label duplicate microscope slides was divided by 2 (Table 1); (K1b) the time to prepare a single Kato-Katz thick smear was directly measured; (K2), (K3) and (K5) the average time of the working steps was divided by 2; (K6d-j) the time was equal for single or duplicate Kato-Katz thick smears; (K11a-c) the time was divided by 2. The time to prepare and read the FLOTAC double technique was assessed as follows: (F2c), (F3-F6), (F10) and (F11b-e) the average time was divided by 2; (F8) this working step was excluded. In the remaining working steps the measured time was applicable for the FLOTAC dual or double technique.
To calculate the total time expenditure for a single and duplicate Kato-Katz thick smears and the FLOTAC dual and double technique, the arithmetic means of the time needed to perform a specific working step were summed-up. Out of the total working time, it was determined how many employees were needed to perform 100 stool examinations per day, assuming that laboratory staff work 7 h per day. Subsequently, the total salary costs for the team were calculated. The costs were divided by 100, to obtain the costs due to salaries per stool examination.
Costs due to materials
Price of material needed for duplicate Kato-Katz thick smears and the FLOTAC dual technique, taking into account the asset cost, life time and days of use.
Asset cost (US$)
Present material (n)
Lost material (n)
Examinations per material (n)
Estimated life expectancy (years)
Estimated days in use per year
Price per stool examination (US$)
FLOTAC dual technique
Falcon tubes (for stool preservation)
5% formaldehyde (1 l)
0.9% NaCl solution (1 l)
FS4 (NaNO3, 1 l)
Falcon tubes (for preparation)
Filter for FLOTAC
FLOTAC apparatus (reading disk)
Balance (Kern EMB basic balance)
Centrifuge (Hettich Universal 320)
Centrifuge (Hettich EBA 3 [second hand])
Duplicate Kato-Katz thick smears
Wire mesh (3 cm2)
Kato-Katz kit (template and plastic spatula)
FLOTAC dual technique and duplicate Kato-Katz thick smears
Container (120 ml) for stool collection
Hand tally counter
Microscope (Olympus CX 21)
Costs due to infrastructure
Costs due to infrastructure originated from (i) the use of the laboratory building for stool examinations (e.g. rent, tap water and electricity); and (ii) the use of the car for stool container distribution and collection in schools. The monthly costs for the laboratory and the asset costs for the 4-WD car, together with the monthly expenditures for car maintenance were reported by the head of HCLU. The life expectancy of a car was derived by consulting WHO-choice  and the petrol costs were assessed on the spot in Zanzibar (average diesel price for March-May 2009).
One-way sensitivity analyses
A series of one-way sensitivity analyses were performed to determine the robustness of the cost estimations and to assess to what extent the costs vary if a specific parameter changes [22–24]. We alternated single parameters pertaining to the total costs of the Kato-Katz or FLOTAC technique. For the scenario indicated in the Appendix, the following parameters were considered: (i) salary; (ii) unproductive time; (iii) costs due to materials; (iv) petrol costs; (v) costs due to infrastructure; and (vi) costs without collection of stool samples in the field.
Parasitological findings and diagnostic sensitivity
Among 1,066 school children screened with duplicate Kato-Katz thick smears, the prevalence of T. trichiura, hookworm and A. lumbricoides was 62.8%, 19.8% and 9.2%, respectively. The FLOTAC double technique was performed on 343 stool samples and revealed respective prevalences of 67.1%, 11.7% and 10.2%. Infection intensities, according to WHO guidelines , were mostly light. Considering the combined results of stool samples examined with duplicate Kato-Katz thick smears and the FLOTAC double technique as diagnostic 'gold' standard, the sensitivity of duplicate Kato-Katz thick smears for detection of T. trichiura, hookworm and A. lumbricoides eggs was 88%, 81% and 68%, respectively. The respective sensitivities for FLOTAC were 95%, 54% and 88%.
Costs due to salaries
In 2009 the daily salary of an employee at HCLU was, on average, US$ 18.83 (minimum: US$ 17.65, maximum US$ 22.18; excluding managers' salaries).
The average person-time needed to collect one stool sample in the field, including transfer to HCLU was 10 min and 55 sec (10:55 min) (Figure 2 [S1-S3]). The average time to prepare and read duplicate Kato-Katz thick smears was 16:26 min (Figure 2 [K1-K6]) and 09:39 min for a single Kato-Katz thick smear, whereas for the FLOTAC dual technique a total of 25:49 min (Figure 2 [F1-F11]) and for the FLOTAC double technique a total of 17:19 min was recorded. The total time for one stool examination, including stool collection in the schools and transfer to HCLU, was 20:34 min for a single Kato-Katz thick smear, 27:21 min for duplicate Kato-Katz thick smears, 28:14 min for the FLOTAC double technique and 36:44 min for the FLOTAC dual technique.
Including the salary of the workers, the costs due to salaries for one stool examination were US$ 1.25 for a single Kato-Katz thick smear, US$ 1.57 for duplicate Kato-Katz thick smears, US$ 1.64 for the FLOTAC double technique and US$ 1.95 for the FLOTAC dual technique.
Costs due to materials
The prices of all materials used for stool examinations at HCLU together with examinations and assumptions about their life expectancy, are summarized in Table 2. The costs due to materials for single or duplicate Kato-Katz thick smears were US$ 0.03 and US$ 0.04, respectively. For the FLOTAC double and dual technique the costs due to materials were US$ 0.26 and US$ 0.43, respectively.
Costs due to infrastructure
The monthly costs for the use of the laboratory building were reported to be US$ 256.77. Assuming that 3,000 stool samples were examined during 1.5 months (30 working days), the costs of the laboratory building were US$ 0.13 per stool sample. The 4-WD car was bought in 2002 for US$ 22,000. WHO-choice suggests a lifetime for a 4-WD car of 8 years . Considering that the car is in use at 200 days per year, the costs per day were US$ 31.91 (including monthly car maintenance of US$ 51.87 and daily petrol consumption of 15 l diesel). Assuming that 100 stool samples were collected per day, the costs per sample due to the car were US$ 0.32. Hence, the costs due to infrastructure were US$ 0.45 for each diagnostic method in a survey of 30 working days with a total of 3,000 stool samples collected by a research team in a field-setting located ~30 km from the laboratory and examined by a team of experienced laboratory technicians.
One-way sensitivity analyses
Results of the series of one-way sensitivity analyses to illustrate the impact of alternated cost parameters on the total costs of the Kato-Katz and the FLOTAC method for the diagnosis of soil-transmitted helminth infections.
Costs in US$ (change in %)
Single Kato-Katz thick smear
Duplicate Kato-Katz thick smears
FLOTAC double technique
FLOTAC dual technique
Costs due to salaries
Salaries increase by 100%
30% of unproductive time per working step in the laboratory
Costs due to materials
Material costs increase by 100%
Costs due to infrastructure
Petrol costs increase by 100%
Infrastructure costs increase by 100%
Stool samples brought to laboratory by participants
In a scenario where stool samples are not collected in the field (costs of car and field-work excluded (Figure 2 [S1-S3])), the total costs decrease in the range of 31.8% (FLOTAC dual technique) to 56.8% (single Kato-Katz thick smear).
The time requirements and costs for the diagnosis of soil-transmitted helminth infections in a cross-sectional epidemiological survey, using either the Kato-Katz technique performed on fresh stool samples or the FLOTAC technique using preserved stool samples, are considerable. Indeed, in our study, the costs for a single or duplicate Kato-Katz thick smears were US$ 1.73 and US$ 2.06, respectively. As expected, the costs for the FLOTAC technique were even higher: US$ 2.35 for the FLOTAC double technique and US$ 2.83 for the FLOTAC dual technique. The higher costs of the FLOTAC technique are mainly caused by the longer preparation time for stool samples and microscopic examinations in the laboratory. Previous studies have shown that the FLOTAC technique is more sensitive than the Kato-Katz method for the diagnosis of soil-transmitted helminth infections [15–18], and hence higher costs might be justified. In the present study, the FLOTAC outperformed the Kato-Katz method only for A. lumbricoides and T. trichiura diagnosis, but was seemingly less sensitive for hookworm diagnosis, for reasons reported elsewhere (Knopp S, Speich B, Rinaldi L, Mohammed KA, Khamis IS, Mohammed AZ, Albonico M, Rollinson D, Marti H, Cringoli G, Utzinger J: "Diagnostic accuracy of the Kato-Katz and FLOTAC techniques when used for assessing anthelmintic drug efficacy", submitted).
In view of the high costs to collect a single stool sample in the field and subsequent examination in the laboratory, preventive chemotherapy without prior diagnosis, as advocated by WHO for high-risk groups in endemic settings is, at first sight, justified [3, 26]. In endemic settings, where morbidity control is shifting to infection and transmission control, an accurate assessment of the epidemiological situation is required and renders diagnosis necessary [5, 7]. For evaluating the efficacy of routinely applied and newly developed drugs, as well as for individual patient management, accurate diagnostic tools are undoubtedly needed [5, 7]. Whenever diagnosis for soil-transmitted helminthiasis is warranted, our results can be of value to decision makers and scientists in budget-planning for epidemiological surveys and to heads of diagnostic laboratories for patient management.
It is important to note that in our study, the Kato-Katz method was performed by rigorously adhering to the WHO bench aids . Indeed, all Kato-Katz thick smears were read twice, first 20-40 min after preparation for hookworm and 3-6 h later a second time for A. lumbricoides and T. trichiura. While this procedure increases costs, it results in higher sensitivity of the Kato-Katz method and might explain the differences in the diagnostic performance of the Kato-Katz and FLOTAC techniques compared to previous comparative investigations [15–18]. Routinely, however, Kato-Katz thick smears are read only once for the three common soil-transmitted helminth species, which saves costs, but also decreases sensitivity.
Moreover, our data are derived from a large survey, where many stool samples were collected and processed simultaneously. One-way sensitivity analyses showed that costs decrease by more than 50% if, for example, individual patients are managed at hospital or public health centre laboratories, without a need for a field team and a 4-WD car. However, in those laboratories, stool samples are not necessarily examined in large numbers, and hence cost and time are likely higher than presented here, due to economy of scale issues . Of note, additional expenses for the patient will then arise from travel costs and loss of income due to missed working hours .
Furthermore, in our setting, the prevalences of A. lumbricoides and hookworm were low and infection intensities of all soil-transmitted helminth species were light. Hence, the counting of helminth eggs by microscopists was relatively quick. In settings with higher prevalence and infection intensities, quantitative microscopic examination will be more time consuming and costs will rise.
Of additional cost relevance is that salaries usually constitute the largest part of the total service costs . Our sensitivity analysis indicates that an alteration of salaries is impacting most on the total costs of both the Kato-Katz and FLOTAC method. In contrast to the Kato-Katz, the total costs of FLOTAC are also strongly influenced by alterations in material costs. It should be noted that the costs for material maintenance (e.g. centrifuge and microscope), the sourcing of chemicals and the adequate disposal of hazardous chemicals were not included in our cost calculations. Moreover, transfer of large equipment (e.g. centrifuge) and costs that might be incurred at customs were not considered in our analyses. Highly setting-specific and dependant on economy are infrastructural costs including rent, electricity and petrol.
In our study, the time to prepare and microscopically examine a stool sample using the FLOTAC double technique was 17:19 min. This result is in line with the time of 21 min reported in a recent study, where the FLOTAC technique was applied for the diagnosis of Fasciola hepatica in rats . However, another study applying FLOTAC for the diagnosis of non-human Trichuris infections revealed a preparation and reading time of only 09:48 min . Of note, our laboratory workers were newly trained to use the FLOTAC method and it is likely that they might have performed faster if the method was being applied routinely. Hence, costs would have been saved. On the other side, the costs of more extensive training necessary to learn the FLOTAC technique in comparison with the simple training for the Kato-Katz method were not considered in our analyses.
Another potential bias in our study is that that the laboratory workers were constantly supervised by an external researcher and that their working speed was recorded. It is hence possible that the staff's working speed differed from normal activity . It is also widely acknowledged that every employee has unproductive phases during a normal working day . To account for unproductive working time, we added 30% of the total measured working time for all working steps in the laboratory in one of the sensitivity analyses. This increased the total costs of the diagnostic methods by up to 18%. The working steps in the field (Figure 2, S1-S3) were excluded from the calculations since unproductive time was already included in the respective time measurements (time record of departure from, and arrival at, the laboratory). Since it is well known that employees make conscious and unconscious breaks during the working day , we consider the cost estimations including 30% of unproductive working time closer to reality. For the reason that in our study workers were observed and because unproductive time was not included in our baseline calculation, our cost estimates are rather conservative.
Despite that the cost estimates for the Kato-Katz and FLOTAC method from our study are not readily transferable to other epidemiological settings where additional costs might occur, or costs might be saved, we believe that they give a reasonable idea of the expenses related to soil-transmitted helminth diagnosis and can guide scientists and decision makers in budget planning for epidemiological surveys. The real costs of diagnosis are of considerable relevance to large-scale control programmes, which often need to balance the costs of treatments against the costs of diagnosis. As global funds to control soil-transmitted helminth infections become more widely available this will be an increasingly important issue to ensure that control programmes are cost-effective and sustainable.
We are grateful to all participating children and the headmasters of Kinyasini and Kilombero schools and would like to thank all members of the HCLU and the Ministry of Health and Social Welfare for their help in the field and in the laboratory. We acknowledge the support of Dr. Marti for assessing costs and life expectancy of laboratory materials. This investigation received financial support from the Commission of Research Partnerships with Developing Countries (through the SDC-sponsored program "Jeunes Chercheurs" to S. Knopp), the Swiss National Science Foundation (project no. PPOOB--102883 and PPOOB--119129), and the European Union (FP6STREP CONTRAST project, contract no. 032203). S. Knopp was also supported by the Burckhardt Foundation Basel (personal stipend for the final year of her PhD studies). B. Speich received financial support from the Swiss Tropical and Public Health Institute (teaching and training).
Scenario of epidemiological survey to illustrate the effect of changing cost parameters on the total costs of the Kato-Katz and FLOTAC technique.
Screening of stool samples obtained from 3,000 schoolchildren for soil-transmitted helminth infections within 30 working days. One hundred children are screened per day. One working day consists of a maximum of 7 working hours.
To assess how much the alteration of a single parameter can influence the total costs of a single or duplicate Kato-Katz thick smears and of the FLOTAC double or dual technique for diagnosing soil-transmitted helminths.
In a series of one-way sensitivity analyses it was estimated how the total costs for one stool examination with the Kato-Katz or FLOTAC method change under the following assumptions:
• Costs due to salaries:
- the salaries of employees are doubled
- 30% of unproductive working time is added to each laboratory working step
• Costs due to materials:
- all material costs are doubled
• Costs due to infrastructure:
- the petrol price is doubled
- costs of the whole infrastructure is doubled
- stool samples are submitted directly to a laboratory (field-related costs from driving to schools and collecting stool samples, as well as the costs for a 4-WD car are omitted)
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