Gyrodactylus salinae n. sp. (Platyhelminthes: Monogenea) infecting the south European toothcarp Aphanius fasciatus (Valenciennes) (Teleostei, Cyprinodontidae) from a hypersaline environment in Italy
© Paladini et al; licensee BioMed Central Ltd. 2011
Received: 31 March 2011
Accepted: 9 June 2011
Published: 9 June 2011
Historically, non-native species of Gambusia (Poeciliidae) have been used to control larval stages of the Asian tiger mosquito, Stegomyia albopicta Reinert, Harbach et Kitching, 2004 throughout Italy. The potential utility of indigenous populations of Aphanius fasciatus (Valenciennes) (Teleostei: Cyprinodontidae) as an appropriate alternative biological control is currently being explored. A sub-sample of ten fish collected from Cervia Saline, Italy (salinity 65 ppt; 30°C) to assess their reproductive capability in captivity, harboured a moderate infection of Gyrodactylus von Nordmann, 1832 (Platyhelminthes, Monogenea). A subsequent morphological and molecular study identified this as being a new species.
Gyrodactylus salinae n. sp. is described from the skin, fins and gills of A. fasciatus. Light and scanning electron microscopical (SEM) examination of the opisthaptoral armature and their comparison with all other recorded species suggested morphological similarities to Gyrodactylus rugiensoides Huyse et Volckaert, 2002 from Pomatoschistus minutus (Pallas). Features of the ventral bar, however, permit its discrimination from G. rugiensoides. Sequencing of the nuclear ribosomal DNA internal transcribed spacers 1 and 2 and the 5.8S rRNA gene and a comparison with all species listed in GenBank confirmed they are unique and represent a new species (most similar to Gyrodactylus anguillae Ergens, 1960, 8.3% pair-wise distance based on 5.8S+ITS2) . This represents the first species of Gyrodactylus to be described from Aphanius and, to date, has the longest ITS1 (774 bp) sequenced from any Gyrodactylus. Additional sampling of Cervia Saline throughout the year, found G. salinae n. sp. to persist in conditions ranging from 35 ppt and 5°C in December to 65 ppt and 30°C in July, while in captivity a low level of infection was present, even in freshwater conditions (0 ppt).
The ability of G. salinae n. sp. to tolerate a wide range of salinities and temperatures shows its potential to readily adapt to several environmental conditions. These findings, together with the fact that A. fasciatus is a protected species and is considered as a biological control organism, necessitate further studies on the ecology and virulence of G. salinae n. sp.
Toothcarp is a colloquial term used to describe members of the order Cyprinodontiformes, which comprises ten fish families, namely Anablepidae, Aplocheilidae, Cyprinodontidae, Fundulidae, Goodeidae, Nothobranchiidae, Poeciliidae, Profundulidae, Rivulidae and Valenciidae . Within the family Cyprinodontidae, the south European toothcarp Aphanius fasciatus (Valenciennes) is one of the more commonly occurring neritic species within the Mediterranean and is characteristically known to be an eurythermic and euryhaline fish species, for its ability to tolerate a wide range of temperatures (5-39°C) and salinities (0-180 ppt), respectively . Aphanius fasciatus has a widespread distribution along the Italian coastline, principally in brackish waters , although increasing anthropogenic activity has caused a general decline in numbers. For this reason, A. fasciatus is considered to be a species that is "dying out" and as such, is listed under Appendix III "Protected Fauna Species" after the Bern Convention on the conservation of European wildlife and natural habitats, and under Annex II of the Council Directive 92/43/EEC on the conservation of natural habitats and of wild fauna and flora in the European Community . It has recently been used as a biological control against the larvae of the Asian tiger mosquito Stegomyia albopicta (= Aedes albopictus) Reinert, Harbach et Kitching, 2004 which are vectors for a range of human infectious diseases, including Chikungunya fever, dengue fever, West Nile fever and yellow fever [5–7].
During a recent research study investigating the artificial reproduction of A. fasciatus in captivity as part of a large scale restocking and mosquito control initiative in Italy [8, 9], several specimens collected, under licence, on a number of occasions were found to harbour an infection of Gyrodactylus von Nordmann, 1832, principally on the skin and fins and, to a lesser degree, on the gills. Infected tissues were removed from the moribund fish and subsequently sent to the Institute of Aquaculture, University of Stirling (Scotland, UK) for identification. Given the importance of A. fasciatus as a protected species and its utility as an alternative indigeneous biological control agent to the introduced Gambusia spp. (Poeciliidae), more information on its Gyrodactylus fauna was needed.
Specimens collection and preparation
A total of ten adult A. fasciatus (total length 4-7 cm; weight ~3 g) was collected during July 2008 from isolated pools in Cervia Saline, located in the Emilia Romagna region in northern Italy, and fixed in 70% ethanol. All ten specimens collected from the drying, landlocked pools were moribund individuals, a consequence of reduced water availability and increased algal growth. The skin, fins and gills of each fish were subsequently screened for metazoan parasites using an Olympus SZ40 stereomicroscope at ×4 magnification. Specimens of Gyrodactylus were removed using mounted triangular surgical needles. All ten fish were infected but given the condition of the fish on collection, the intensity of infection can only be estimated at between 10-30 parasites per fish.
The alcohol-fixed parasites were subsequently rinsed in distilled water and representatives prepared as whole mounts using ammonium picrate glycerine following the procedure detailed by Malmberg . Additional specimens had their opisthaptors removed using a scalpel, which were then individually subjected to proteolytic digestion on glass slides, as described in Paladini et al. . The largely tissue-free opisthaptoral hook preparations were then mounted in ammonium picrate glycerine using an 18 × 18 mm coverslip, the edges of which were sealed with a commercial brand of nail varnish. The corresponding body of each specimen of Gyrodactylus was fixed in 90% ethanol for subsequent molecular characterisation.
For scanning electron microscopy (SEM), single specimens of Gyrodactylus were subjected to full proteolytic digestion on 11 mm round glass coverslips to obtain tissue-free attachment hooks. Each digestion took approximately 60 min, with 3 μl of digestion solution being added every 10 min, punctuated by the addition of 5 μl distilled water for five times to remove digested tissue residues and debris. For each step, the digested hook preparations were placed in a Petri dish to protect them from extraneous dust and placed in an incubator at 55°C to help digestion, followed by a final incubation at 40°C overnight to dry. The position of hooks on each coverslip was subsequently marked using tiny, adhesive, triangular labels positioned using forceps under an Olympus BX51 compound microscope at ×10 magnification. The coverslips were then attached onto aluminium stubs with bi-adhesive round labels, sputter-coated with gold using an Edwards S150B sputter coater and then examined using a JEOL JSM5200 SEM operating at an accelerating voltage of 10 kv.
For the morphological study, images of the opisthaptoral hard parts and the male copulatory organ (MCO) were captured at magnifications of ×40 and ×100 oil immersion using MRGrab 220.127.116.11 (Carl Zeiss Vision GmbH, 2001) software and a Zeiss AxioCam MRc digital camera mounted on an Olympus BX51 compound microscope, using a ×0.75 interfacing lens. Drawings of the taxonomic features were made from the captured images. Each Gyrodactylus specimen was subjected to morphometric analysis taking a total of 27 point-to-point measurements on the opisthaptoral hooks using a JVC KY-F30B 3CCD video camera mounted on an Olympus BH2 microscope using a ×2.5 interfacing lens at ×100 oil immersion and the KS300 (ver.3.0) (Carl Zeiss Vision GmbH, 1997) image analysis software, combined with the specific macro for gyrodactylids, Point-R (Bron & Shinn, University of Stirling). The point-to-point hook measurements for the specimens are given in micrometres as the mean ± 1 standard deviation followed by the range in parentheses, and follow those described in Shinn et al. , plus three additional measurements of the dorsal bar (total length, width and attachment point length).
The gyrodactylid material prepared from A. fasciatus was compared to type material of Gyrodactylus rugiensoides Huyse et Volckaert, 2002 (paratypes acc. nos. BMNH 2002.2.14.2-3), a species with morphologically similar marginal hooks, held in the Parasitic Worms collection at The Natural History Museum, London, UK. In addition, type material of the six Gyrodactylus species known to parasitise cyprinodontid hosts, held in the U.S. National Parasite Collection, Beltsville, Maryland, USA, was examined, the marginal hooks re-drawn and compared to the new specimens collected from A. fasciatus. These are Gyrodactylus cyprinodontis Mizelle et Kritsky, 1967 (holotype and paratype acc. no. USNPC 62951), Gyrodactylus hargisi Williams et Rogers, 1971 (paratypes acc. no. USNPC 71760), Gyrodactylus mobilensis Williams et Rogers, 1971 (paratypes acc. no. USNPC 71762), Gyrodactylus nevadensis Mizelle et Kritsky, 1967 (holotype and paratype acc. no. USNPC 62954), Gyrodactylus saratogensis Mizelle et Kritsky, 1967 (paratype acc. no. USNPC 62956) and Gyrodactylus tularosae Kritsky et Stockwell, 2005 (paratype acc. no. USNPC 94780).
The bodies of 2 specimens were individually transferred to a 0.2 ml microcentrifuge tube containing 5 μl of milli-Q water and digested by the addition of 5 μl of lysis solution consisting of 1×PCR buffer (Eurogentec, Seraing, Belgium), 0.45% (v/v) Tween 20, 0.45% (v/v) NP 40 and 60 μg/ml of proteinase K (Sigma, Poole, UK). The samples were incubated at 65°C for 25 min, followed by 10 min at 95°C to inactivate the proteinase. The primer pairs ITS1A (5'-GTAACAAGGTTT CCGTAGGTG-3') and ITS2 (5'-TCCTCCGCTTAGTGATA- 3')  were used to amplify a fragment spanning the 3' end of the 18S rRNA gene, the internal transcribed spacer 1 (ITS1), the 5.8S rRNA gene, ITS2, and the 5' end of the 28S rRNA gene. The amplification reactions (20 μl) consisted of 1×PCR buffer, 1.5 mM MgCl2 (Eurogentec, Seraing, Belgium), 200 μM of each dNTP (Amersham Pharmacia Biotech, Uppsala, Sweden), 1 μM of each primer (Eurogentec, Seraing, Belgium), 2 μl lysate, 1 unit Taq polymerase (Eurogentec, Seraing, Belgium) and milli-Q water. The mixtures were heated for 4 min at 96°C and subjected to 35 cycles of 1 min at 95°C, 1 min at 50°C and 2 min at 72°C, followed by a final extension at 72°C for 7 min. The PCR products were visualised using ethidium bromide on a 1.2% agarose gel. The products were then purified by means of GFX columns according to the manufacturer's instructions (Amersham Pharmacia Biotech, Uppsala, Sweden). Both DNA strands were sequenced using a Big Dye Chemistry Cycle Sequencing Kit (version 1.1) in a 3130 DNA Analyzer (Applied Biosystems, Belgium). The PCR primers and 2 internal primers, ITS1R (5'-ATTT GCGTTCGAGAGACCG-3') and ITS2F (5'-TGGTGGATCA CTCGGCTCA-3') , were used for sequencing.
The obtained sequences were subjected to a BLAST search (available at http://www.ncbi.nih.gov/BLAST/) to identify similar sequences among other species of Gyrodactylus in GenBank . The sequences with the highest similarity to our sequences were downloaded and the 5.8S and ITS2 fragments were aligned in Clustal W implemented in MEGA 4 . Pair-wise genetic distances were computed in MEGA 4 according to the evolutionary model that was selected by jModelTest 0.1.1 . The sequences were also scanned for repeat elements using the program Tandem Repeats Finder .
Gyrodactylus salinae n. sp
Aphanius fasciatus (Valenciennes), Cyprinodontidae ("South European toothcarp", "nono").
Site of infection
Skin, fins and occasionally gills.
Cervia Saline, Emilia Romagna region, Italy (44°14'N, 12°20'E).
Environmental conditions under which specimens were collected
Salinity and temperature in July 2008, 65 ppt and 30°C, respectively.
Fifteen specimens were studied for light microscopy. Holotype (acc. no. BMNH 2011.5.19.1) and four paratypes (acc. nos. BMNH 2011.5.19.2-5) are deposited in the parasitic worm collection at The Natural History Museum, London. Additionally, three paratypes (acc. no. M-521) are deposited in the gyrodactylid collection held at the Institute of Parasitology, Academy of Sciences of the Czech Republic, České Budĕjovice; four paratypes (acc. nos. AHC 35118-35121) are deposited in the Australian Helminthological Collection (AHC) of The South Australian Museum (SAMA), North Terrace, Adelaide; and three paratypes (acc. nos. USNPC 104748-104750) are deposited in the United States National Parasite Collection, Beltsville, Maryland, USA.
Molecular sequence data
The sequence fragment of approximately 1379 bp encoding partial 18S (32 bp), ITS1 (774 bp), 5.8S (158 bp), ITS2 (402 bp) and partial 28S (13 bp) is deposited in GenBank under accession no. JF950559.
Named after the Italian generic name for a hypersaline water body i.e. "salina" (= saline in English) and the broad salinity tolerance exhibited by this species of Gyrodactylus.
Morphological measurements (mean ± 1 standard deviation followed by the range in parentheses) of Gyrodactylus salinae n. sp. from Aphanius fasciatus (Valenciennes) collected from Cervia Saline, Italy, compared with those of Gyrodactylus rugiensoides Huyse et Volckaert, 2002 from Pomatoschistus minutus (Pallas) collected from Texel, The Netherlands (paratypes acc. nos. BMNH 2002.2.14.2-3)
Gyrodactylus salinae n. sp.
(n = 15)
Gyrodactylus rugiensoides Huyse et Volckaert, 2002
(n = 2)
Total body length
447 ± 63.3 (375-575)
Total body width
116 ± 26.7 (88-163)
Opisthaptor length × width
75 ± 8.9 (60-88) × 80 ± 5.7 (70-88)
112.5 (110-115) × 147.5 (145-150)
Anterior pharynx bulb length × width
18.8 ± 3.1 (15.0-24.5) × 22.1 ± 2.4 (19.5-27.2)
32.9 (30.1-35.7) × 48.3 (46.8-49.7)
Posterior pharynx bulb length × width
8.1 ± 0.7 (6.6-8.8) × 24.6 ± 2.4 (21.8-29.0)
34.1 (27.7-40.5) × 78.4 (76.5-80.4)
MCO length × width
14.4 ± 2.1 (11.9-18.5) × 12.7 ± 2.7 (10.1-17.6)1
14.8 (13.6-15.9) × 15.1 (14.0-16.1)
17.9 ± 0.8 (17.0-19.6)
H proximal shaft width
7.0 ± 0.6 (6.3-8.1)
H point length
24.8 ± 0.5 (23.9-25.9)
H distal shaft width
4.1 ± 0.4 (3.2-4.6)
H shaft length
31.9 ± 2.9 (28.2-37.3)
H inner curve length
3.8 ± 0.6 (2.3-4.6)
H aperture angle (°)
36.6 ± 1.2 (33.8-37.9)
H point curve angle (°)
12.2 ± 2.3 (7.4-15.4)
Inner H aperture angle (°)
41.4 ± 1.3 (39.2-43.5)
H root length
16.8 ± 0.9 (14.7-18.3)
H total length
51.7 ± 1.6 (48.7-54.6)
Dorsal bar (DB)
DB total length
20.9 ± 1.7 (18.1-22.9)
1.9 ± 0.2 (1.6-2.2)
DB attachment point length
9.4 ± 0.4 (8.8-9.9)
Ventral bar (VB)
VB total width
25.0 ± 1.1 (23.5-26.7)
VB total length
22.5 ± 1.6 (19.2-25.5)
VB process-to-mid length
3.0 ± 0.5 (2.1-4.0)
VB median length
6.2 ± 0.4 (5.6-6.8)
VB process length
2.9 ± 0.4 (2.1-3.5)
VB membrane length
13.2 ± 1.1 (10.7-15.2)
Marginal hook (MH)
MH total length
26.8 ± 0.6 (25.9-27.6)
MH shaft length
20.8 ± 0.3 (20.2-21.5)
MH sickle length
6.3 ± 0.2 (6.1-6.6)
MH sickle proximal width
4.0 ± 0.2 (3.6-4.4)
MH toe length
1.8 ± 0.1 (1.6-2.0)
MH sickle distal width
3.6 ± 0.2 (3.3-4.0)
5.6 ± 0.2 (5.4-6.0)
MH instep/arch height
0.6 ± 0.1 (0.5-0.7)
Based on the measurements of fifteen specimens. Body elongate, 447 (375-575) long, 116 (88-163) wide. Prohaptor with a single pair of cephalic lobes each bearing a gland and a spike sensillum. Pharynx small, anterior pharyngeal bulb 18.8 (15.0-24.5) long, 22.1 (19.5-27.2) wide; posterior pharyngeal bulb 8.1 (6.6-8.8) long, 24.6 (21.8-29.0) wide. Intestinal crura extending beyond the anterior edge of the testes. Presence or absence of excretory bladders not discernible on whole mounts. Opisthaptor ovate, 75 (60-88) long × 80 (70-88) wide. Male copulatory organ (MCO) spherical, 14.4 (11.9-18.5) long × 12.7 (10.1-17.6) wide, armed with one principal spine and 9 small spines in a single row. MCO position variable, usually on the left, posterior to the posterior pharyngeal bulb. Total length of hamuli 51.7 (48.7-54.6); hamulus shaft length 31.9 (28.2-37.3); hamulus point 24.8 (23.9-25.9) long, arising at an angle of 41.4° (39.2-43.5°) (internal measurement) to the shaft of the hamulus; hamulus root 16.8 (14.7-18.3) long. Dorsal bar attachment points on hamuli 9.4 (8.8-9.9) long. Dorsal bar simple, 20.9 (18.1-22.9) long, 1.9 (1.6-2.2) wide irregular and slightly thickened at its mid-point. Ventral bar triangular in approximate dimensions, 22.5 (19.2-25.5) long, 25.0 (23.5-26.7) wide; ventral bar processes prominent, arise tangentially to the extremities of the median portion of the ventral bar proper, 2.9 (2.1-3.5) long; ventral bar membrane long, approximately triangular with a smoothly rounded terminal edge, 13.2 (10.7-15.2) long. Total length of marginal hooks 26.8 (25.9-27.6); marginal hook shaft 20.8 (20.2-21.5) long; marginal hook sickle proper 6.3 (6.1-6.6) long. Sickle shaft approximately perpendicular to the base, very slightly angled forward, proportionately slender, turns at a near right angle into a narrow tip which terminates at a point beyond the perpendicular limit of the toe. Sickle distal width 3.6 (3.3-4.0), proximal width 4.0 (3.6-4.4). Aperture of marginal sickle, open, 5.6 (5.4-6.0) long, inner curve of the sickle approximately rectangular. Sickle base has a flat bridge; triangular toe 1.8 (1.6-2.0) long; prominent rounded heel, tangential to the sickle base, downwardly directed. The sickle heel appears square in dimensions in specimens prepared for light microscopy but SEM images reveal a rounded heel with an additional prominent button for muscle attachment (Figure 1b-e).
Pair-wise genetic distances based on the 5.8S and ITS2 rDNA fragment of Gyrodactylus salinae n. sp. and the Gyrodactylus species showing highest similarity in the BLAST search on GenBank (Tamura-Nei + gamma model)
1. G. salinae n. sp.
2. G. anguillae
3. G. rugiensis
4. G. micropsi
5. G. cf. micropsi
6. G. rugiensoides
7. G. eyipayipi
8. G. longidactylus
Morphologically, the opisthaptoral hooks, notably the marginal hook sickles, of G. salinae n. sp. are similar to those of G. rugiensoides described from the sand goby Pomatoschistus minutus (Pallas) collected from Texel, The Netherlands  (Figure 2d-g). Given the potential overlapping distribution of these two fish hosts within the Mediterranean Sea , it is important to detail the subtle features in hook morphology that permit their discrimination from one another. Two paratypes of G. rugiensoides were examined for morphological comparison with G. salinae n. sp. The paratypes were re-measured and the marginal hook sickle re-drawn and overlaid with G. salinae n. sp. for a direct comparison (Table 1; Figure 2e-g). There was a good agreement between the measurements obtained in the current study and those presented in Huyse & Volckaert . The present study, however, provides an additional set of measurements taken from the type material of G. rugiensoides for direct comparison with the opisthaptoral features of G. salinae n. sp. Although the hamuli roots in both species narrow after their union with the shaft, giving the anterior edge of the dorsal bar attachment point a small but distinct edge, this appears to be more prominent in G. rugiensoides than on the hamuli of G. salinae n. sp. The ventral bar attachment points also differ; those of G. rugiensoides appear flat and rectangular, while those of G. salinae n. sp. are indented. The prominent ventral bar processes and the longer, slender ventral bar membrane of G. salinae n. sp. contributes to the discrimination of the two species. The similar morphology of the marginal hook sickles of both species though, requires careful examination. The union of the marginal hook shaft with the sickle divides the width of the sickle base into 3:2 (heel:toe) in both species, however, the sickle base is deeper in G. rugiensoides than in G. salinae n. sp. with a more angular, rectangular heel and a steeper faced, more robust toe (Figure 2f-g). The sickle shaft and sickle tip of G. rugiensoides is broader, proportionately so, than that of G. salinae n. sp. giving the latter the appearance of having a more open deeper sickle aperture. A comparison of soft body features suggests that the posterior pharynx bulb of G. rugiensoides (78.4 μm in diameter) is considerably larger than that of G. salinae n. sp. which measures in 24.6 μm in diameter. Although Huyse & Volckaert  described the MCO of G. rugiensoides as armed with one principal spine and five small spines, a closer examination of the paratypes shows that G. rugiensoides possesses nine small spines, as that of G. salinae n. sp. The MCOs of the two species are similar in length (G. salinae n. sp. 14.4 μm vs G. rugiensoides 14.8 μm) but that of G. rugiensoides is slightly wider (12.7 vs 15.1).
A molecular comparison of G. rugiensoides and G. salinae n. sp. showed that they were quite distinct. The pair-wise distance amounted to 14% (based on the 5.8S + ITS2 fragment). The ITS1 sequences were more difficult to align, due to length differences (up to 132 bp). Both species belong to the so-called marine rugiensis-group that also includes G. anguillae.
Additional sampling A. fasciatus from the Cervia Saline at several time points throughout the year, found that G. salinae n. sp. was present on their hosts in waters ranging from 35 ppt and 5°C during December to 65 ppt and 30°C during July in the wild, while under captive conditions, fish even maintained a low level of infection in freshwater (0 ppt) (pers. obs.).
Gyrodactylus species parasitising different family members belonging to the order Cyprinodontiformes
Recorded host species
G. avalonia *
Fundulus diaphanus diaphanus (Lesueur)
Fundulus zebrinus (Jordan et Gilbert)
Poecilia mexicana Steindachner, Poecilia reticulata Peters, Poecilia sphenops Valenciennes, Xiphophorus hellerii Heckel, X. hellerii × Xiphophorus maculatus (Günther) hybrids
Epiplatys fasciolatus (Günther)
Cyprinodon nevadensis nevadensis Eigenmann et Eigenmann
Aplocheilichthys eduardensis (David et Poll), Aplocheilichthys normani Ahl, Aplocheilichthys pumilus (Boulenger)
Fundulus similis (Baird et Girard)
Gambusia affinis (Baird et Girard)
Cyprinodon variegatus variegatus Lacepède
Girardinichthys multiradiatus (Meek)
Poecilia caucana (Steindachner)
Cyprinodon v. variegatus
Cyprinodon n. nevadensis
Micropoecilia picta Regan
Aplocheilus blockii (Arnold), Aplocheilus panchax (Hamilton)
G. salinae n. sp.
Aphanius fasciatus (Valenciennes)
Cyprinodon n. nevadensis
Fundulus d. diaphanus
Fundulus grandis Baird et Girard, Fundulus heteroclitus heteroclitus (L.)
Cyprinodon tularosa Miller et Echelle
P. reticulata, Poeciliopsis sp.
Heterandria bimaculata (Heckel)
A list of all the species of Gyrodactylus recorded from cyprinodontids and other fish hosts belonging to the order Cyprinodontiformes is presented in Table 3. In addition to these, there are a number of other "Gyrodactylus" species parasitising cyprinodontiform hosts that appear in the literature. These include Gyrodactylus foxi Rawson, 1973, Gyrodactylus megacanthus Wellborn et Rogers, 1967, Gyrodactylus prolongis Hargis, 1955, Gyrodactylus stableri Hathaway et Herlevich, 1973 and Gyrodactylus trematoclithrus Rogers, 1967, all of which were subsequently transferred to the genus Fundulotrema Kritsky et Thatcher, 1977 based on the presence of an additional peduncular bar .
The fact that the current species is (both morphologically and molecularly) more closely related to Gyrodactylus species parasitising marine gobies and eels than to those infecting cyprinodontids, might suggest that the ecology of the host, rather than host phylogeny plays an important role in this host-parasite system. Malmberg  found G. anguillae only on migrating elvers, which are relatively small and abundantly found in estuaries, as is the common goby Pomatoschistus microps (Krøyer), host to G. micropsi. There are other examples of ecological radiations onto distant-related hosts in Gyrodactylus, e.g. the G. wageneri-group primarily infects cyprinids but they are also found on sticklebacks, percids and cottids .
Based on the nuclear ITS fragment, the genus Gyrodactylus can be divided in two groups, one with a 'short' (347-473 bp) and one with a 'long' (535-688 bp) ITS1 fragment [30, 31]. These ranges have recently been extended both to the lower end, with Gyrodactylus cichlidarum Paperna, 1968 having the shortest ITS1 sequence determined thus far (343 bp; acc. no. DQ124228), and G. salinae n. sp. having the longest ITS1 fragment (774 bp). The sequence of G. salinae n. sp. is also unique as the 5.8S gene is 158 bp long, whereas all other Gyrodactylus species so far had an invariant length of 157 bp. The new species clusters in the marine rugiensis-group which is part of the monophyletic 'long ITS' group, and appears most closely related to G. anguillae. Despite the morphological resemblance between G. salinae n. sp. and G. rugiensoides, they are genetically quite different (especially in the ITS1 fragment). This underlines again the fact that morphological and molecular evolution is not always correlated. The close affinity with G. anguillae and Gyrodactylus cf. micropsi Huyse, Audenaert et Volckaert, 2003 as based on the genetic distances does not necessarily equal sister species relationships. The true sister species of G. salinae n. sp. has probably not been sequenced yet since the difference between Gyrodactylus species from closely related goby species was for example much smaller (2-5%)  than the distance between G. anguillae and G. salinae n. sp.
Although the specimens of A. fasciatus from Cervia Saline were moribund at the time of collection, this was not a consequence of the moderate G. salinae n. sp. infection but of the pool conditions (30°C, 65 ppt) from which they were collected which had a water depth of less than 15 cm and a heavy growth of algae. Given the ability of G. salinae n. sp. to tolerate a wide range of salinities and temperatures, it would appear that this species has the potential to readily adapt to the full spectrum of environmental conditions. What is unknown, however, is the potential of this species to survive on other neritic fish hosts when cohabiting isolated pools or interacting with other fish species in open, full strength seawater (i.e. 35 ppt). The potential utility of Aphanius fasciatus as a biological control against the larvae of the Asian tiger mosquito Stegomyia albopicta, in addition to the finding of a new parasite species, raises concerns regarding the health status of this host due to the parasitic infection. Further studies are required in order to define the potential role of G. salinae n. sp. as a pathogen, and the consequences that this finding might have on the use of indigenous populations of A. fasciatus as an alternative biological control to the use of various Gambusia species. The ecological risks of introducing Gambusia spp. to non-native environments has been stressed by many authors [33–35]. Cross breeding with indigenous species may lead to the extinction of the latter given that certain species have been shown to hybridise [36–38]. There are also the attendant risks of disease introduction linked with the movement of fish into new habitats . The use of native fish, rather than introduced species, as biological controls, therefore, is highly recommended to avoid these potential ecological impacts.
In accordance with section 8.6 of the ICZN's International Code of Zoological Nomenclature, copies of this article are deposited at the following five publicly accessible libraries: Natural History Museum, London, UK; American Museum of Natural History, New York, USA; Museum National d'Histoire Naturelle, Paris, France; Russian Academy of Sciences, Moscow, Russia; Academia Sinica, Taipei, Taiwan.
The authors would like to thank Mr Antonio Casalini, Mr Giuseppe Bastone and Dr Oliviero Mordenti (Faculty of Veterinary Medicine, University of Bologna, Italy) for providing literature and moribund fish for study; Mr Linton Brown (Institute of Aquaculture, University of Stirling, UK) for technical assistance with the acquisition of scanning electron photomicrographs; Mrs Eileen Harris (The Natural History Museum, London, UK) and Mrs Patricia Pilitt (United States National Parasite Collection, Beltsville, Maryland, USA) for the loan of type material from international collections. This study was conducted during the tenure of a PhD scholarship from the Department of the Environment, Food and Rural Affairs (Defra), UK (project no. FC1183) awarded to APS and conducted by GP. TH is currently a Postdoctoral Fellow of the Research Foundation - Flanders (FWO-Vlaanderen).
- Froese R, Pauly D: FishBase, World Wide Web electronic publication. 2010, last access 12/2010, [http://www.fishbase.org]Google Scholar
- Triantafyllidis A, Leonardos I, Bista I, Kyriazis ID, Stoumboudi MT, Kappas I, Amat F, Abatzopoulos TJ: Phylogeography and genetic structure of the Mediterranean killifish Aphanius fasciatus (Cyprinodontidae). Mar Biol. 2007, 152: 1159-1167. 10.1007/s00227-007-0760-7.View ArticleGoogle Scholar
- Mordenti O, Trentini M, Bastone G, Savoia L, Scaravelli D: Caratterizzazioni ecologiche e morfologiche di Aphanius fasciatus (Valencienne, 1821). Biol Mar Medit. 2008, 15 (1): 306-307 [In Italian with English summary].Google Scholar
- Crivelli AJ:Aphanius fasciatus. IUCN 2010 IUCN Red List of Threatened Species. 2006, Version 2010.4 (last access 03/2011), [http://http//www.iucnredlist.org]Google Scholar
- Miller BR, Ballinger ME: Aedes albopictus mosquitoes introduced into Brazil: vector competence for yellow fever and dengue viruses. Trans R Soc Trop Med Hyg. 1988, 82 (3): 476-477. 10.1016/0035-9203(88)90168-X.View ArticlePubMedGoogle Scholar
- Sardelis MR, Turell MJ, O'Guinn ML, Andre RG, Roberts DR: Vector competence of three North American strains of Aedes albopictus for West Nile virus. J Am Mosq Control Assoc. 2002, 18 (4): 284-289.PubMedGoogle Scholar
- Cinti S: Chikungunya fever: an emerging infection. Infect Dis Clin Pract. 2009, 17 (1): 6-11. 10.1097/IPC.0b013e31818a15e5.View ArticleGoogle Scholar
- Mordenti O, Scaravelli D, Trentini M, Zaccaroni A, Gamberoni M: Reproduction of Aphanius fasciatus induced by ecophysiological conditioning and hormonal treatment. Biol Mar Medit. 2007, 14 (2): 276-277 [In Italian with English summary].Google Scholar
- Mordenti O, Scaravelli D, Trentini M: Prove di controllo su Aedes albopictus (Skuse, 1894): sperimentazione di alimentazione su larve da parte di Aphanius fasciatus (Valencienne, 1821) (Cyprinodontiformes, Cyprinodontidae). Proc XXII Congr Naz Ital Entomol, 15th-18th June 2009, Ancona (Italy):. 2009, 267 [In Italian]-June , Ancona (Italy):Google Scholar
- Malmberg G: The excretory systems and the marginal hooks as a basis for the systematics of Gyrodactylus (Trematoda, Monogenea). Ark Zool. 1970, 23: 1-235.Google Scholar
- Paladini G, Gustinelli A, Fioravanti ML, Hansen H, Shinn AP: The first report of Gyrodactylus salaris Malmberg, 1957 (Platyhelminthes, Monogenea) on Italian cultured stocks of rainbow trout (Oncorhynchus mykiss Walbaum). Vet Parasitol. 2009, 165: 290-297. 10.1016/j.vetpar.2009.07.025.View ArticlePubMedGoogle Scholar
- Shinn AP, Hansen H, Olstad K, Bachmann L, Bakke TA: The use of morphometric characters to discriminate species of laboratory-reared and wild populations of Gyrodactylus salaris and G. thymalli (Monogenea). Folia Parasitol. 2004, 51: 239-252.View ArticlePubMedGoogle Scholar
- Matĕjusová I, Gelnar M, McBeath AJA, Collins CM, Cunningham CO: Molecular markers for gyrodactylids (Gyrodactylidae: Monogenea) from five fish families (Teleostei). Int J Parasitol. 2001, 31: 738-745. 10.1016/S0020-7519(01)00176-X.View ArticlePubMedGoogle Scholar
- Ziętara MS, Lumme J: Speciation by host switch and adaptive radiation in a fish parasite genus Gyrodactylus (Monogenea, Gyrodactylidae). Evolution. 2002, 56: 2445-2458.View ArticlePubMedGoogle Scholar
- Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ: Basic local alignment search tool. J Mol Biol. 1990, 215: 403-410.View ArticlePubMedGoogle Scholar
- Tamura K, Dudley J, Nei M, Kumar S: MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol. 2007, 24: 1596-1599. 10.1093/molbev/msm092.View ArticlePubMedGoogle Scholar
- Posada D: jModelTest: phylogenetic model averaging. Mol Biol Evol. 2008, 25: 1253-1256. 10.1093/molbev/msn083.View ArticlePubMedGoogle Scholar
- Benson G: Tandem repeats finder: a program to analyze DNA sequences. Nucl Acids Res. 1999, 27: 573-580. 10.1093/nar/27.2.573.PubMed CentralView ArticlePubMedGoogle Scholar
- Shinn AP, Harris PD, Cable J, Bakke TA, Paladini G, Bron JE: GyroDb. World Wide Web electronic publication. 2011, last access 03/2011, [http://www.gyrodb.net]Google Scholar
- Shinn AP, Paladini G, Rubio-Godoy M, Domingues MV, Whittington ID, Bron JE: MonoDb. A web-host for Monogenea. World Wide Web electronic publication. 2011, last access 03/2011, [http://www.monodb.org]Google Scholar
- Tamura K, Nei M: Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol. 1993, 10: 512-526.PubMedGoogle Scholar
- Huyse T, Volckaert FAM: Identification of a host-associated species complex using molecular and morphometric analyses, with the description of Gyrodactylus rugiensoides n. sp. (Gyrodactylidae, Monogenea). Int J Parasitol. 2002, 32: 907-919. 10.1016/S0020-7519(02)00026-7.View ArticlePubMedGoogle Scholar
- Mizelle JD, Kritsky DC: Studies on monogenetic trematodes. XXXVI. Gyrodactylid parasites of importance to California fishes. Calif Fish Game. 1967, 53: 264-272.Google Scholar
- Paladini G, Cable J, Fioravanti ML, Faria PJ, Shinn AP: The description of Gyrodactylus corleonis sp. n. and G. neretum sp. n. (Platyhelminthes: Monogenea) with comments on other gyrodactylids parasitising pipefish (Pisces: Syngnathidae). Folia Parasitol. 2010, 57 (1): 17-30.View ArticlePubMedGoogle Scholar
- Rubio-Godoy M, Paladini G, García-Vásquez A, Shinn AP: Gyrodactylus jarocho sp. nov. and Gyrodactylus xalapensis sp. nov. (Platyhelminthes: Monogenea) from Mexican poeciliids (Teleostei: Cyprinodontiformes), with comments on the known gyrodactylid fauna infecting poeciliid fish. Zootaxa. 2010, 2509: 1-29.Google Scholar
- Kritsky DC, Thatcher VE: Phanerothecium gen. nov. and Fundulotrema gen. nov. two new genera of viviparous Monogenoidea (Gyrodactylidae), with a description of P. caballeroi sp. nov, and a key to the subfamilies and genera of the family. Excerta Parasitol Mem Dr Eduardo Caballero y Caballero Inst Biol Publ Esp. 1977, 4: 53-60.Google Scholar
- Harris PD: Interactions between reproduction and population biology in gyrodactylid monogeneans - a review. Bull Fr Pêche Piscic. 1993, 328: 47-65.View ArticleGoogle Scholar
- Hayward CJ, Iwashita M, Crane JS, Ogawa K: First report of the invasive eel pest Pseudodactylogyrus bini in North America and in wild American eels. Dis Aquat Org. 2001, 44: 53-60.View ArticlePubMedGoogle Scholar
- Grano-Maldonado MI, Gisbert E, Hirt-Chabbert J, Paladini G, Roque A, Bron JE, Shinn AP: An infection of Gyrodactylus anguillae Ergens, 1960 (Monogenea) associated with the mortality of glass eels (Anguilla anguilla L.) on the north-western Mediterranean Sea board of Spain. Vet Parasitol. 2011,Google Scholar
- Cable J, Harris PD, Tinsley RC, Lazarus CM: Phylogenetic analysis of Gyrodactylus spp. (Platyhelminthes: Monogenea) using ribosomal DNA sequences. Can J Zool. 1999, 77: 1439-1449.View ArticleGoogle Scholar
- Ziętara MS, Huyse T, Lumme J, Volckaert FAM: Deep divergence among subgenera of Gyrodactylus inferred from rDNA ITS region. Parasitology. 2002, 124: 39-52.PubMedGoogle Scholar
- Huyse T, Malmberg G, Volckaert FAM: Four new species of Gyrodactylus von Nordmann, 1832 (Monogenea, Gyrodactylidae) on gobiid fishes: combined DNA and morphological analyses. Sys Parasitol. 2004, 59: 103-120.View ArticleGoogle Scholar
- Leyse KE, Lawler SP, Strange T: Effects of an alien fish, Gambusia affinis, on an endemic California fairy shrimp, Linderiella occidentalis: implications for conservation of diversity in fishless waters. Biol Conserv. 2004, 118: 57-65. 10.1016/j.biocon.2003.07.008.View ArticleGoogle Scholar
- García-Berthou E, Alcaraz C, Pou-Rovira Q, Zamora L, Coenders G, Feo C: Introduction pathways and establishment rates of invasive aquatic species in Europe. Can J Fish Aquat Sci. 2005, 62: 453-463. 10.1139/f05-017.View ArticleGoogle Scholar
- Reichard M, Watters BR, Wildekamp RH, Sonnenberg R, Nagy B, Polačik M, Valdesalici S, Cellerino A, Cooper BJ, Hengstler H, Rosenstock J, Sainthouse I: Potential negative impacts and low effectiveness in the use of African annual killifish in the biocontrol of aquatic mosquito larvae in temporary water bodies. Parasit Vectors. 2010, 3: 89. 10.1186/1756-3305-3-89.PubMed CentralView ArticlePubMedGoogle Scholar
- Ryan MJ, Wagner WE: Asymmetries in mating preferences between species: female swordtails prefer heterospecific males. Science. 1987, 236: 595-597. 10.1126/science.236.4801.595.View ArticlePubMedGoogle Scholar
- Rosenfield J, Kodric-Brown A: Sexual selection promotes hybridization between Pecos pupfish, Cyprinodon pecosensis-C. variegatus and sheepshead minnow. J Evol Biol. 2003, 16: 595-606. 10.1046/j.1420-9101.2003.00557.x.View ArticlePubMedGoogle Scholar
- Wong BBM, Fisher HS, Rosenthal GG: Species recognition by male swordtails via chemical cues. Behav Ecol. 2005, 16: 818-822. 10.1093/beheco/ari058.View ArticleGoogle Scholar
- Daszak P, Cunningham AA, Hyatt AD: Emerging infectious diseases of wildlife - threats to biodiversity and human health. Science. 2000, 287: 443-449. 10.1126/science.287.5452.443.View ArticlePubMedGoogle Scholar
- Dechtiar A, Christie WJ: Survey of the parasite fauna of Lake Ontario fishes, 1961-1971. Parasites of Fishes in the Canadian Waters of the Great Lakes Great Lakes Fishery Commission Technical Report no 51. Edited by: Nepszy SJ. 1988, Ann Arbor, Michigan, 66-95.Google Scholar
- Mayes MA: New species of Gyrodactylus and Dactylogyrus (Trematoda: Monogenea) from fishes of Nebraska. J Parasitol. 1977, 63: 805-809. 10.2307/3279882.View ArticlePubMedGoogle Scholar
- Turnbull ER: Gyrodactylus bullatarudis n. sp. from Lebistes reticulatus Peters with a study of its life-cycle. Can J Zool. 1956, 34: 583-594. 10.1139/z56-061.View ArticleGoogle Scholar
- Harris PD: Species of Gyrodactylus von Nordmann, 1832 (Monogenea: Gyrodactylidae) from poeciliid fishes, with a description of G. turnbulli sp. nov. from the guppy, Poecilia reticulata Peters. J Nat Hist. 1986, 20: 183-191. 10.1080/00222938600770151.View ArticleGoogle Scholar
- Dove ADM, Ernst I: Concurrent invaders - four exotic species of Monogenea now established on exotic freshwater fishes in Australia. Int J Parasitol. 1998, 28: 1755-1764. 10.1016/S0020-7519(98)00134-9.View ArticlePubMedGoogle Scholar
- Kritsky DC, Fritts TH: Monogenetic trematodes from Costa Rica with the proposal of Anacanthocotyle gen. n. (Gyrodactylidae: Isancistrinae). Proc Helminthol Soc Wash. 1970, 37: 63-68.Google Scholar
- Paperna I: Monogenetic trematodes collected from freshwater fishes in Ghana. Second report. Bamidgeh. 1968, 20: 88-90.Google Scholar
- Hargis WJ: Monogenetic trematodes of Gulf of Mexico fishes. Part I. The superfamily Gyrodactyloidea. Biol Bull. 1955, 108: 125-137. 10.2307/1538429.View ArticleGoogle Scholar
- Rogers WA, Wellborn TL: Studies on Gyrodactylus (Trematoda: Monogenea) with descriptions of five new species from the southeastern U.S. J Parasitol. 1965, 51: 977-982. 10.2307/3275887.View ArticlePubMedGoogle Scholar
- Williams EH, Rogers WA: Two new species of Gyrodactylus (Trematoda: Monogenea) and a redescription and new host record for G. prolongis Hargis, 1955. J Parasitol. 1971, 57: 845-847. 10.2307/3277811.View ArticleGoogle Scholar
- Mendoza-Palmero CA, Sereno-Uribe AL, Salgado-Maldonado G: Two new species of Gyrodactylus von Nordmann, 1832 (Monogenea: Gyrodactylidae) parasitizing Girardinichthys multiradiatus (Cyprinodontiformes: Goodeidae), an endemic freshwater fish from central Mexico. J Parasitol. 2009, 95: 315-318. 10.1645/GE-1761.1.View ArticlePubMedGoogle Scholar
- Harris PD, Cable J: Gyrodactylus poeciliae n. sp. and G. milleri n. sp. (Monogenea: Gyrodactylidae) from Poecilia caucana (Steindachner) in Venezuela. Sys Parasitol. 2000, 47: 79-85. 10.1023/A:1006413804061.View ArticleGoogle Scholar
- Cable J, van Oosterhout C, Barson N, Harris PD: Gyrodactylus pictae n. sp. (Monogenea: Gyrodactylidae) from the Trinidadian swamp guppy Poecilia picta Regan, with a discussion on species of Gyrodactylus von Nordmann, 1832 and their poeciliid hosts. Sys Parasitol. 2005, 60: 159-164. 10.1007/s11230-004-6348-4.View ArticleGoogle Scholar
- Lucký Z: Gyrodactylus rasini n. sp. (Monogenoidea: Gyrodactylidae) a parasite on the gills of Xiphophorus hellerii bred as an aquarium fish in Czechoslovakia. Vet Med. 1973, 18: 647-652.Google Scholar
- Rukmini C, Madhavi R: Gyrodactylus recurvensis n. sp. (Monogenea, Gyrodactylidae) from larvivorous fishes Aplocheilus panchax and A. blocki. Indian J Helminthol. 1989, 6: 17-20.Google Scholar
- Mueller JF: Further studies on North American Gyrodactyloidea. Am Midl Nat. 1937, 18: 207-219. 10.2307/2420497.View ArticleGoogle Scholar
- King SD, Cone DK: Morphological and molecular taxonomy of a new species of fundulotrema and comments on Gyrodactylus stephanus (Monogenea: Gyrodactylidae) from Fundulus heteroclitus (Actinopterygii: Cyprinodontiformes) in Nova Scotia, Canada. J Parasitol. 2009, 95 (4): 846-849. 10.1645/GE-1901.1.View ArticlePubMedGoogle Scholar
- Kritsky DC, Stockwell CA: New species of Gyrodactylus (Monogenoidea, Gyrodactylidae) from the white sands pupfish, Cyprinodon tularosa, in New Mexico. Southwest Nat. 2005, 50 (3): 312-317. 10.1894/0038-4909(2005)050[0312:NSOGMG]2.0.CO;2.View ArticleGoogle Scholar
- An L, Jara CA, Cone DK: Five species of Gyrodactylus Nordmann, 1832 (Monogenea) from fresh-water fishes of Peru. Can J Zool. 1991, 69: 1199-1202. 10.1139/z91-171.View ArticleGoogle Scholar
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