- Open Access
Assessment of exposure to Plasmodium falciparum transmission in a low endemicity area by using multiplex fluorescent microsphere-based serological assays
© Sarr et al; licensee BioMed Central Ltd. 2011
- Received: 20 July 2011
- Accepted: 7 November 2011
- Published: 7 November 2011
The evaluation of malaria transmission intensity is a crucial indicator for estimating the burden of malarial disease. In this respect, entomological and parasitological methods present limitations, especially in low transmission areas. The present study used a sensitive multiplex assay to assess the exposure to Plasmodium falciparum infection in children living in an area of low endemicity. In three Senegalese villages, specific antibody (IgG) responses to 13 pre-erythrocytic P. falciparum peptides derived from Lsa1, Lsa3, Glurp, Salsa, Trap, Starp, Csp and Pf11.1 proteins were simultaneously evaluated before (June), at the peak (September) and after (December) the period of malaria transmission, in children aged from 1 to 8 years.
Compared to other antigens, a high percentage of seropositivity and specific antibody levels were detected with Glurp, Salsa1, Lsa3NR2, and Lsa1J antigens. The seropositivity increased with age for all tested antigens. Specific IgG levels to Glurp, Salsa1, Lsa3NR2, and Lsa1J were significantly higher in P. falciparum infected children compared to non-infected and this increase is significantly correlated with parasite density.
The multiplex assay represents a useful technology for a serological assessment of rapid variations in malaria transmission intensity, especially in a context of low parasite rates. The use of such combined serological markers (i.e. Glurp, Lsa1, Lsa3, and Salsa) could offer the opportunity to examine these variations over time, and to evaluate the efficacy of integrated malaria control strategies.
- Malaria Transmission
- Malaria Infection
- Parasite Density
- Negative Control Group
Plasmodium falciparum malaria is a major cause of human morbidity and mortality in sub-Saharan Africa, and its transmission varies greatly in endemicity across the continent . The expanding utilization of combined malaria control strategies including insecticide impregnated bednets and artemisinin combination therapies, has contributed to greatly reduce malaria transmission in several sub-Saharan African areas [2, 3]. Consequently, the current methods for evaluating malaria transmission intensity (MTI), such as entomological inoculation rate and Plasmodium parasitemia in human populations, present substantial limitations, e.g. reproducibility and can be time-consuming. In addition, both entomological and parasitological measures are affected by the seasonality and require a precise follow-up during longitudinal studies . For this purpose, there is an increased need for developing new tools for the monitoring of MTI in more frequent contexts of low malaria transmission. In this respect, the sero-epidemiological approach offers a theoretical advantage over parasite prevalence for assessing MTI or changes in prevalence following the implementation of control programmes . In order to identify Plasmodium infections, serological markers show greater sensitivity, as seroprevalence reflects cumulative exposure to infections and thus is less affected by the changes in parasite densities, which could be undetectable in the case of very low parasite density. Previous studies showed that serological measurements are robust to detect short term variations in transmission, and should be a pertinent tool for evaluating malaria exposure in the context of low transmission .
The Circumsporozoite protein (CSP: a protein expressed by sporozoites and early liver forms), has been frequently used for the serological estimation of MTI . Controversial studies have reported that antibody (Ab) responses directed to the repetitive NANP domains of CSP remained very low throughout the first year of life . In addition, it has been suggested that human immunological memory following malaria infection is short-lived because Ab responses rapidly decline after the end of the transmission season or exposure period , and after treatment of a clinical episode . It suggests that maintenance of immunological memory therefore requires antigen persistence and may be age-dependent . For this purpose, it has been then demonstrated that the simultaneous use of several antigens (Ags) as serological markers could lead to a better evaluation of malaria exposure than using only one Ag, i.e. CSP . Human Ab levels to plasmodial Ags are classically assessed using the enzyme-linked immunosorbant assay (ELISA) test. This technique is labour-intensive and time-consuming, as well as requiring considerable quantity of Ags and sera samples. An immunoassay that measures Ab to multiple Ags simultaneously would be highly advantageous . Multiplexed bead assays gives similar sensibility than ELISA assays, and have been developed in several studies for simultaneous detection of Ab against multiple plasmodial Ags in humans living in endemic areas [14, 15].
In the present study, we used multiplex fluorescent microsphere-based assays measuring simultaneously human Ab to thirteen P. falciparum peptides  to assess malaria transmission in children living in a low endemicity area. All Ags used in the assay have previously been shown to be antigenic and associated with malaria transmission in individuals living in malaria endemic areas [6, 14].
The study was performed in the villages of Mboula (Ferlo area: 15°40' N, 15°25' W), Gankette Balla (near the Guiers Lake: 15°58' N, 15°55' W) and Mbilor (Low valley area: 16°29' N, 15°33' W) in Northern Senegal, located along the Senegal Basin River . This site is a dry savannah area, with approximately 400 mm of rain by year. Malaria transmission is low (average of 3 to 7 infective bites per human per year), markedly seasonal (occurring from July to October), with a peak in September .
Three cross-sectional surveys were performed in June, September and December 2004 in the three villages. For each passage, a sub-cohort of children aged from 1 to 8 years was randomly selected from an existing cohort of 450 children, as previously described . A total of 186 children (Mboula n = 64; Gankette n = 56 and Mbilor n = 66, selected for the 3 passages) were included in the present study. For each child, parasitological measurements of malaria were performed at each passage using thick blood smears obtained by finger-prick. The smears were Giemsa stained to identify Plasmodium species and the number of malaria parasites was counted in 200 microscopic fields. Parasite density was defined as the number of P. falciparum parasites/μl of blood, corresponding to an average of 8000 leucocytes. In the same way, capillary blood was collected from each child at each passage for immunological assessments.
The present study followed ethical principles according to the Helsinki Declaration, and was approved by the Ethics Committee of the Ministry of Health of Senegal (CNRS, June 2004). Informed consent was obtained from the parents/tutors of the children.
Multiplex bead-based assay
The multiplex technique was performed using the same sequences of P. falciparum peptides (Lsa1-41, Lsa1J, Lsa3NR2, Lsa3RE, Glurp, Glurp.P3, Salsa1, Salsa2, Trap1, Trap2, StarpR, Csp, Sr11.1), and the procedure for coupling Ags to beads, as previously described . Briefly, the P. falciparum peptides were synthesized with an added N-terminal cysteine residue and covalently coupled with bovine serum albumin by Genepep (Ales, France). These Ags were coupled to beads (Biorad Inc, CA, USA) and 50 μl/well was deposited at the final concentration of 80 beads/μl per peptide. Diluted individual sera (1/100) in equal volumes of PBS and MFIA (Multiplex Fluorescence ImmunoAssay) diluents (Charles River Laboratories Inc, MA, USA) were added in duplicate using 50 μl/well. The Luminex system was set for reading a minimum of 100 beads per spectral address, and results were expressed as ΔMFI "median fluorescent intensity" value with ΔMFI = MFIAg - MFIBSA, where MFIAg represents the mean of individual MFI value for beads coupled with P. falciparum Ag, and MFIBSA the individual MFI value for each serum for beads without coupled Ag. For calculating the seropositivity threshold, the means and standard deviations (SD) of ΔMFI of individuals living permanently in a non-endemic area (i.e. negative control group - n = 19) were estimated for each Ag. The lower limit of positivity of Ab responses to one Ag was estimated as the mean of ΔMFI of the negative control group + 3.09 SD. Therefore, values higher than the cut-offs should be observed in less than 1% of sera of non-exposed individuals under the hypothesis of normally distributed of ΔMFI. The thresholds of positivity were 7466.6, 684.0, 725.0, 225.0, 344.2, 4593.1, 424.8, 439.7, 435.2, 933.6, 452.6, 380.2, and 302.5 respectively for Lsa1-41, Lsa1J, Lsa3NR2, Lsa3RE, Glurp, Glurp.P3, Salsa1, Salsa2, Trap1, Trap2, StarpR, Csp, and SR11.1. Individual time-period variations in Ab levels for each Ag were assessed by the ratio of ΔMFI values collected at different time intervals, i.e. between June - September; September - December or June - September. The significant increase in Ab level to each Ag was defined as a value superior to the mean + 3.09 SD of the ratio in ΔMFI in the negative control group collected two times at four months interval.
All data were analyzed with Graph Pad Prism® (Graph Pad, San Diego, USA) version 4. After checking for normal distribution, the proportions of Ab-positive individuals and Ab levels were analyzed using the chi-squared test, Mann-Whitney U-test, Kruskal-Wallis test, where appropriate. Spearman's correlation was used to check for correlations between continuous variables. Differences were considered significant at P < 0.05.
Proportion and number of seropositive according to the period of malaria transmission and village.
Mboula (n = 64)
Gankette (n = 56)
Mbilor (n = 66)
All villages (n = 186)
Some of these antigens (Glurp, Lsa1J, Lsa3NR2, and Salsa1) appeared to satisfy several of the requirements expected for a relevant marker of MTI. These peptides are antigenic and provided a higher incidence rate of significant increases in IgG levels. Specific Ab levels to these Ags are closely associated with the presence and the intensity of malaria infection. Moreover, in children seropositive to at least one peptide, 86.3% (120 of 139) were seropositive to at least one out of these four peptides. Pre-erythrocytic Glurp, Lsa1, Lsa3 and Salsa Ags are mainly expressed in infected human hepatocytes, they present little polymorphism from geographically different P. falciparum isolates [23–26]. Recent immuno-epidemiological studies in different endemic areas, have shown a positive association of specific Ab directed to these Ags and the level of malaria transmission . Our results strengthen that pre-erythrocyte Ags, and particularly Glurp, Lsa1, Lsa3, and Salsa, could be pertinent markers to assess and to survey malaria transmission, at the individual level and in the context of low P. falciparum prevalence. Nevertheless, further studies will be valuable for validating these potential serological markers. These studies could include future modelling exercises including covariates factors such as age, genetic background, village of residence, impact of vector/parasite control programmes and the period of transmission. This kind of tool could allow elaborating a precise picture of malaria epidemiology in large-scale areas, or be used as a complementary indicator for evaluating the efficacy of integrated malaria control strategies.
In conclusion, the multiplex assay provides a useful tool to simultaneously measured Ab responses directed to several Ags used as potential markers of malaria transmission. In low transmission areas, serological measurements to various malaria antigens are needed for estimating short term and small scale variations in MTI. However, the present study suggests that the combined assessment of Ab levels to only Glurp, Lsa1, Lsa3, and Salsa Ags could be a pertinent serological marker for evaluating the MTI.
The authors gratefully acknowledge the populations of Mboula, Mbilor and Gankette, and all the health care agents of these three villages for their participation in the study. The PAL-Fleuve programme was supported by the EPLS GNO, the Délégation Générale pour l'Armement (DGA-CO0co406 "Salivaplus" and 03co009-05 "Impact-Vector"), the French Ministry of Research (PAL+ program) and the Research Institute for Development. The authors thank the FSD "Fonds Social de Développement", from the Embassy of France in Senegal for their financial participation.
- Snow RW, Omumbo JA, Lowe B, Molyneux CS, Obiero JO, Palmer A, Weber MW, Pinder M, Nahlen B, Obonyo C: Relation between severe malaria morbidity in children and level of Plasmodium falciparum transmission in Africa. Lancet. 1997, 349: 1650-1654. 10.1016/S0140-6736(97)02038-2.View ArticlePubMedGoogle Scholar
- Guerra CA, Hay SI, Lucioparedes LS, Gikandi PW, Tatem AJ, Noor AM, Snow RW: Assembling a global database of malaria parasite prevalence for the Malaria Atlas Project. Malar J. 2007, 6: 17-10.1186/1475-2875-6-17.PubMed CentralView ArticlePubMedGoogle Scholar
- O'Meara WP, Bejon P, Mwangi TW, Okiro EA, Peshu N, Snow RW, Newton CR, Marsh K: Effect of a fall in malaria transmission on morbidity and mortality in Kilifi, Kenya. Lancet. 2008, 372: 1555-1562. 10.1016/S0140-6736(08)61655-4.PubMed CentralView ArticlePubMedGoogle Scholar
- Kelly-Hope LA, McKenzie FE: The multiplicity of malaria transmission: a review of entomological inoculation rate measurements and methods across sub-Saharan Africa. Malar J. 2009, 8: 19-10.1186/1475-2875-8-19.PubMed CentralView ArticlePubMedGoogle Scholar
- Drakeley C, Cook J: Chapter 5. Potential contribution of sero-epidemiological analysis for monitoring malaria control and elimination: historical and current perspectives. Adv Parasitol. 2009, 69: 299-352.View ArticlePubMedGoogle Scholar
- Orlandi-Pradines E, Penhoat K, Durand C, Pons C, Bay C, Pradines B, Fusai T, Josse R, Dubrous P, Meynard JB: Antibody responses to several malaria pre-erythrocytic antigens as a marker of malaria exposure among travelers. Am J Trop Med Hyg. 2006, 74: 979-985.PubMedGoogle Scholar
- Webster HK, Gingrich JB, Wongsrichanalai C, Tulyayon S, Suvarnamani A, Sookto P, Permpanich B: Circumsporozoite antibody as a serologic marker of Plasmodium falciparum transmission. Am J Trop Med Hyg. 1992, 47: 489-497.PubMedGoogle Scholar
- Kitua AY, Urassa H, Wechsler M, Smith T, Vounatsou P, Weiss NA, Alonso PL, Tanner M: Antibodies against Plasmodium falciparum vaccine candidates in infants in an area of intense and perennial transmission: relationships with clinical malaria and with entomological inoculation rates. Parasite Immunol. 1999, 21: 307-317. 10.1046/j.1365-3024.1999.00230.x.View ArticlePubMedGoogle Scholar
- Langhorne J, Ndungu FM, Sponaas AM, Marsh K: Immunity to malaria: more questions than answers. Nat Immunol. 2008, 9: 725-732.View ArticlePubMedGoogle Scholar
- Kinyanjui SM, Conway DJ, Lanar DE, Marsh K: IgG antibody responses to Plasmodium falciparum merozoite antigens in Kenyan children have a short half-life. Malar J. 2007, 6: 82-10.1186/1475-2875-6-82.PubMed CentralView ArticlePubMedGoogle Scholar
- Achtman AH, Bull PC, Stephens R, Langhorne J: Longevity of the immune response and memory to blood-stage malaria infection. Curr Top Microbiol Immunol. 2005, 297: 71-102. 10.1007/3-540-29967-X_3.PubMedGoogle Scholar
- Campo JJ, Whitman TJ, Freilich D, Burgess TH, Martin GJ, Doolan DL: Toward a Surrogate Marker of Malaria Exposure: Modeling Longitudinal Antibody Measurements under Outbreak Conditions. PLoS One. 2011, 6: e21826-10.1371/journal.pone.0021826.PubMed CentralView ArticlePubMedGoogle Scholar
- Fouda GG, Leke RF, Long C, Druilhe P, Zhou A, Taylor DW, Johnson AH: Multiplex assay for simultaneous measurement of antibodies to multiple Plasmodium falciparum antigens. Clin Vaccine Immunol. 2006, 13: 1307-1313. 10.1128/CVI.00183-06.PubMed CentralView ArticlePubMedGoogle Scholar
- Ambrosino E, Dumoulin C, Orlandi-Pradines E, Remoue F, Toure-Balde A, Tall A, Sarr JB, Poinsignon A, Sokhna C, Puget K: A multiplex assay for the simultaneous detection of antibodies against 15 Plasmodium falciparum and Anopheles gambiae saliva antigens. Malar J. 2010, 9: 317-10.1186/1475-2875-9-317.PubMed CentralView ArticlePubMedGoogle Scholar
- Elshal MF, McCoy JP: Multiplex bead array assays: performance evaluation and comparison of sensitivity to ELISA. Methods. 2006, 38: 317-323. 10.1016/j.ymeth.2005.11.010.PubMed CentralView ArticlePubMedGoogle Scholar
- Dia I, Konate L, Samb B, Sarr JB, Diop A, Rogerie F, Faye M, Riveau G, Remoue F, Diallo M, Fontenille D: Bionomics of malaria vectors and relationship with malaria transmission and epidemiology in three physiographic zones in the Senegal River Basin. Acta Trop. 2008, 105: 145-153. 10.1016/j.actatropica.2007.10.010.View ArticlePubMedGoogle Scholar
- Sarr JB, Remoue F, Samb B, Dia I, Guindo S, Sow C, Maiga S, Tine S, Thiam C, Schacht AM: Evaluation of antibody response to Plasmodium falciparum in children according to exposure of Anopheles gambiae or Anopheles funestus vectors. Malar J. 2007, 6: 117-10.1186/1475-2875-6-117.PubMed CentralView ArticlePubMedGoogle Scholar
- Zerpa NC, Wide A, Noda J, Bermudez H, Pabon R, Noya OO: Immunogenicity of synthetic peptides derived from Plasmodium falciparum proteins. Exp Parasitol. 2006, 113: 227-234. 10.1016/j.exppara.2006.01.007.View ArticlePubMedGoogle Scholar
- Stewart L, Gosling R, Griffin J, Gesase S, Campo J, Hashim R, Masika P, Mosha J, Bousema T, Shekalaghe S: Rapid assessment of malaria transmission using age-specific sero-conversion rates. PLoS One. 2009, 4: e6083-10.1371/journal.pone.0006083.PubMed CentralView ArticlePubMedGoogle Scholar
- Lee HW, Moon SU, Ryu HS, Kim YJ, Cho SH, Chung GT, Lin K, Na BK, Kong Y, Chung KS, Kim TS: Usefulness of the recombinant liver stage antigen-3 for an early serodiagnosis of Plasmodium falciparum infection. Korean J Parasitol. 2006, 44: 49-54. 10.3347/kjp.2006.44.1.49.PubMed CentralView ArticlePubMedGoogle Scholar
- Migot-Nabias F, Deloron P, Ringwald P, Dubois B, Mayombo J, Minh TN, Fievet N, Millet P, Luty A: Immune response to Plasmodium falciparum liver stage antigen-1: geographical variations within Central Africa and their relationship with protection from clinical malaria. Trans R Soc Trop Med Hyg. 2000, 94: 557-562. 10.1016/S0035-9203(00)90086-5.View ArticlePubMedGoogle Scholar
- Nebie I, Tiono AB, Diallo DA, Samandoulougou S, Diarra A, Konate AT, Cuzin-Ouattara N, Theisen M, Corradin G, Cousens S: Do antibody responses to malaria vaccine candidates influenced by the level of malaria transmission protect from malaria?. Trop Med Int Health. 2008, 13: 229-237. 10.1111/j.1365-3156.2007.01994.x.View ArticlePubMedGoogle Scholar
- Bottius E, BenMohamed L, Brahimi K, Gras H, Lepers JP, Raharimalala L, Aikawa M, Meis J, Slierendregt B, Tartar A: A novel Plasmodium falciparum sporozoite and liver stage antigen (SALSA) defines major B, T helper, and CTL epitopes. J Immunol. 1996, 156: 2874-2884.PubMedGoogle Scholar
- Fidock DA, Gras-Masse H, Lepers JP, Brahimi K, Benmohamed L, Mellouk S, Guerin-Marchand C, Londono A, Raharimalala L, Meis JF: Plasmodium falciparum liver stage antigen-1 is well conserved and contains potent B and T cell determinants. J Immunol. 1994, 153: 190-204.PubMedGoogle Scholar
- Perlaza BL, Sauzet JP, Balde AT, Brahimi K, Tall A, Corradin G, Druilhe P: Long synthetic peptides encompassing the Plasmodium falciparum LSA3 are the target of human B and T cells and are potent inducers of B helper, T helper and cytolytic T cell responses in mice. Eur J Immunol. 2001, 31: 2200-2209. 10.1002/1521-4141(200107)31:7<2200::AID-IMMU2200>3.0.CO;2-L.View ArticlePubMedGoogle Scholar
- Theisen M, Vuust J, Gottschau A, Jepsen S, Hogh B: Antigenicity and immunogenicity of recombinant glutamate-rich protein of Plasmodium falciparum expressed in Escherichia coli. Clin Diagn Lab Immunol. 1995, 2: 30-34.PubMed CentralPubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.