- Open Access
A Google Earth-based surveillance system for schistosomiasis japonica implemented in the lower reaches of the Yangtze River, China
© Sun et al; licensee BioMed Central Ltd. 2011
- Received: 23 August 2011
- Accepted: 27 November 2011
- Published: 27 November 2011
Due to the success of the national schistosomiasis control programme in China, transmission has been sufficiently reduced in many areas to severely limit identification of areas at risk by conventional snail surveys only. In this study, we imported Google Earth technology and a Global Positioning System (GPS) into the monitoring system for schistosomiasis surveillance of the banks of the Yangtze River in Jiangsu Province, China.
A total of 45 sites were selected and the risk was assessed monthly by water exposure of sentinel mice at these sites from May to September in 2009 and 2010. The results were assembled and broadcast via the Google Earth platform.
The intensity of schistosomiasis transmission showed peaks of risk in June and September of 2009, while there was only one small peak in June in 2010 as the number of detected positive transmission sites dropped dramatically that year thanks to improved mollusciciding. River ports were found to be areas of particular risk, but ferry terminals and other centres of river-related activities were also problematic.
The results confirm that the surveillance system can be rapidly updated and easily maintained, which proves the Google Earth approach to be a user-friendly, inexpensive warning system for schistosomiasis risk.
- Global Position System
- Geographical Information System
- Positive Site
The transmission of schistosomiasis depends on the presence of aquatic or amphibious intermediate snail hosts and the disease is therefore closely related to water-bodies and water courses [1, 2]. The maturation process in the snail is governed by the ambient temperature, while the shedding of cercariae (the infective stage) from the snail not only requires water of proper temperature but also sufficient day light [3, 4]. Once emerged from their snail host, the cercariae move towards the surface, contaminating the water and thus turning the area into one of risk for schistosome infection. When humans and/or other definitive animal hosts are exposed to water under such circumstances, the cercariae home in, penetrate the skin and move through the systemic circulation via the lungs and liver to finally settle in the mesenteric microcirculation .
Schistosomiasis japonica, distributed in China and the Philippines, is one of the three major forms of human schistosomiasis which all belong to the neglected tropical diseases (NTDs) [1, 5]. The morbidity due to infection by Schistosoma japonicum is more severe than that caused by the two other main species infective to humans . The transmission foci of schistosomiasis in China are mainly located in five provinces around the lakes in central China including the middle and lower reaches of the Yangtze River [7–9]. Infected humans and bovines in these areas account for 97.2% and 91.2%, respectively, of the total number of cases in the country . Transmission depends entirely on the distribution of the intermediate snail host Oncomelania hupensis and the seasonal risk of infection falls between May and October in alliance with the annual flooding of the Yangtze River [8, 10].
Specific schistosomiasis surveillance in China was introduced in the late 1980s, when scientists conducted a series of preliminary studies to investigate the usefulness of monitoring methods in regions where transmission interruption had been achieved [11–13]. In the early 1990s, a national surveillance network of schistosomiasis was formally established in combination with the launch of the World Bank Loan Project (WBLP) on schistosomiasis control in China . Since then, long-term, continuous and systematic monitoring was gradually developed and integrated with the national schistosomiasis control programme. Dynamic transmission data, as well as information on environmental factors, are now collected via this surveillance system, making it possible to annually update the epidemic status of schistosomiasis in China [15, 16]. The popularization of computerized network applications, Remote Sensing (RS) and Geographical Information Systems (GIS) technologies towards the end of the 20th century facilitated the introduction of surveillance characterized by rapid assessment and timely feedback [17–19]. GIS and RS have contributed to prediction of the future distribution and transmission of the disease, forecasting the potential impact of global warming on its distribution, and the study of the potential impact of the south-north water transfer project on the transmission of schistosomiasis in China [1, 20].
Both human and livestock infection rates in Jiangsu Province along the lower reaches of the Yangtze River have reached the transmission criterion of < 1% prevalence [21, 22]. Although a very positive development, these low endemicity levels makes it difficult to identify the exact extent of infection risk by conventional surveillance systems. Rapid evaluation of water-bodies and up-to-date risk information is an absolute requirement if transmission is to be further mitigated. To deal with this situation in practise, we propose a novel warning system based on the exposure of sentinel mice to water in areas under surveillance with the spatial data monitored by GIS combined with a Global Positioning System (GPS)  supported by Google Earth software. The overall aim was to explore a new approach to monitoring schistosomiasis japonica in low-endemic areas.
The study focused on finding positive sites (areas at risk for both humans and domestic animals) indicated by infected sentinel mice, along with snails and humans surveyed in parallel.
Forty-five monitoring sites were selected in Jiangsu Province. The sites were located approximately 10 km from each other along both banks of the lower reaches of the Yangtze River. All were infested with O. hupensis snails and frequently approached by livestock and also commonly chosen by humans for various activities. The geographical coordinates of the surveillance sites, provided by GPS instruments (Garmin Map76), is shown in Table 1 and Figures 1 and 2.
Localization of the surveillance sites studied (see also Figure 3)
Surveillance site (Type and name)
Bridge islet, Luhe
Systematic snail surveys of the beaches at the monitoring sites were carried out in the spring of 2009 and 2010. A square frame measuring 0.11 m2 was put on the ground every 20 meters and all snails limited by this boundary were recorded, collected and brought back to the laboratory, where they were crushed and microscopically examined for infection .
Sentinel mice of both genders, weighing 23 ± 3 g (females) and 28 ± 3 g (males), were selected. Wired-mesh, 7 × 7 × 65 cm large, rectangular cages were constructed and divided into five cells of equal size. Pieces of foam plastic were tied to the two ends of each cage to make it float on the water surface. At each surveillance site, two cages (each cell containing 2 mice, each cage 10 mice) were anchored 10-20 m apart. The foam plastic pieces of each cage were adjusted to assure that the abdomen and tail of each mouse would be exposed to the water. Each month during the period May to September, the sentinel mice were left in the cages on the water surface from 10:00 A.M. to 2:00 P.M. for two consecutive days.
After exposure, the mice were collected, indexed and left in the animal department of the laboratory at an ambient temperature of 22-26°C and a relative humidity of 40-70%. They were killed after 35-40 days (to allow maturation of any infecting schistosome), and dissected taking care to inspect the portal and mesenteric veins for worms and the liver for eggs. Mice with eggs in the liver and/or presence of adult worms were recorded as positive for infection.
Data management, presentations and electronic dissemination were supported by Google Earth, version 5.0 and the photographic images of the surveillance sites saved in the photo gallery by Picasa, version 3.1 (Google). For each surveillance site, a database was prepared that included information on location (latitude and longitude), snail distribution and the monitoring results for each month. In addition, the sites were entered as 'landmarks' into Google Earth by coordinates using red colour for positives and yellow for negatives.
Information regarding the schistosomiasis infection was regularly sent to the local disease control centre via the Google Earth platform in parallel to being sent to higher levels. A detailed workplan for the local schistosomiasis control was developed and scheduled to be set in motion in case of positive results. It required that professional, technical teams be sent to the sites within 24 hours to survey the environment and establish specific control measures. The plan further required that treatment and protection of high-risk populations be applied within three days and that an evaluation of the response efficacy was prepared within one week (Figure 3).
Surveillance results in the 45 surveillance sites along the Yangtze River
Time of exposure
Infection rate (%)
12-13 May 2009
10-11 June 2009
10-11 July 2009
10-11 Aug 2009
6-7 Sept 2009
30-31 May 2010
18-19 June 2010
14-15 July 2010
12-13 Aug 2010
9-10 Sept 2010
With regard to the surveillance sites, a total of 12 positive ones, including one duplicate record (site found positive at two separate occasions), were detected in 2009, i.e. 24.44% (11/45) or almost a quarter of all sites were at risk that year. There was a small peak in May/June and a strong peak in September mirroring the temporal distribution of infected mice (Figure 4). In 2010 in contrast, only three positive surveillance sites, again including one duplicate were detected over the whole surveillance period, and only in May and June (Table 2).
Surveillance results in relation to type of site
Type of site
Number of sites
Infection rate (%)d
New sand bank
The surveillance results were electronically transmitted five times per year (one for each month of the season) to local and superior levels through the Google Earth surveillance system. This was not only done for research and surveillance purposes but also to ensure that emergency measures such as mollusciciding and cercarial elimination were carried out to protect the local population in case of detected risk. The technical staff at the health authorities of the counties (cities, districts) where positive sentinel mice were detected went to the field and screened all high risk populations. A total of 2,968 people were tested serologically by dipstick immunoassay provided by JIPD. Chemotherapy was given to 417 people who tested positively. Niclosamide ethanolamine was applied as cercariacidal to around 25,700 m2 suspected water surfaces and health education was delivered to the people frequenting sites found to be positive. Warning signs were set up along the river banks and protection ointment against schistosome infection was distributed to all considered to be at risk.
In the spring of 2010, mollusciciding was twice applied at the 11 positive sites detected in 2009. In August, 2010, Jiangsu launched a propaganda week of schistosomiasis control in the sensitive regions along the Yangtze River, subjecting a total of 26,421 people of the mobile population working on the river to health examinations with a special focus on schistosomiasis.
The Google Earth and GPS technologies were introduced into the monitoring system for schistosomiasis, in order to 1) visualize the spatiotemporal aspects of the monitoring approach, 2) understand the transmission dynamics of schistosomiasis along the Yangtze River, and 3) provide rapid risk updates for the purpose of immediate emergency responses.
Schistosomiasis japonica impacts human health seriously, hindering social and economic development . After six decades of successful prevention, China has made great progress in schistosomiasis control. The number of infected people has dropped from the initial 11.6 million in 1950s to 0.4 million currently, a decrease of 96.65% [6, 26]. Around 80% of all endemic counties (districts, cities) have met the criteria for transmission interruption and control [7, 27] and surveillance is rapidly becoming an important part of the national schistosomiasis control programme.
As we arrive at different control stages, the monitoring content, methods and indicators change . While surveillance in the early 1980's was less developed and relatively passive [11–13], an active system based on defined monitoring methods and indicators has now been implemented [15, 28]. By the start of the 21st century, 80 fixed surveillance sites are in use for longitudinal monitoring within the endemic areas [9, 29]. At the same time, as a supplement to these fixed sites, mobile units have been introduced at the provincial level.
To improve and standardize the approach, and at the same time saving time and money, we integrated the Google Earth software including an operative platform and the use of sentinel mice to establish a modern warning system. Application of this system over two transmission seasons (2009-2010) has proved that this user-friendly approach is inexpensive, yet easily maintained, so lending itself to continuous updating.
This study covered 200 km along the Yangtze River in Jiangsu province and took place between May and September in 2009 and 2010. The overall positive rates of sentinel mice for the two years was 0.4% which is considerably lower than that (26.6%) recorded in 2003 based on 26 surveillance sites in Nanjing, the capital of Jiangsu Province . The results clearly confirm that schistosomiasis transmission has been significantly reduced in Jiangsu province since the implementation of the long-term control plan by the Jiangsu government [21, 22].
Two infection peaks were detected in 2009 which is consistent with previous reports [31, 32]. This two-peak transmission pattern can be explained by the periodic evolvement of infected snails. The lifespan of the intermediate host snail is around one year and each year, in the early transmission season (May/June), snails infected during the previous transmission season contaminate the water-bodies. During the flooding that starts in July, most of this infected older snail generation die off, while a new generation becomes infected. The sporocyst schistosome stage in these newly infected snails mature in August and the snails start shedding cercariae in September. The June peak was never remarkable compared to the September one since the routinely applied large-scale, spring mollusciciding programme regularly reduces the number of previously infected snails, while the new generation is produced later in the year and is therefore less affected. Consequently, the emergence of new, infective snails between August and October along the river puts the area at risk for schistosomiasis infection and emphasizes the importance of applying control measures at this time of the year as well as in the spring. This was done in 2010, which resulted in only one small peak at the early surveillance stage that year and led to the dramatic reduction in risk in spatiotemporal terms (number of positive sites and months at risk) compared to that of the previous year.
In conclusion, the improved situation was attributed to the following reasons: 1) reinforced mollusciciding control strategy at the hotspots in the spring as well as in the autumn of 2010; 2) health control propaganda in August 2010 with emphasis on the mobile population working on the Yangtze River attempting to reduce contamination of the water with schistosome eggs; and 3) screening and treatment of target populations.
We found the most dangerous environments for schistosome infection to be the river ports, ferry terminals and public vessel centres with human activities. Enhanced prevention and control efforts should target these areas. Interestingly, two surveillance sites with positive sentinel mice had no snails or a very low density of snails and this situation demands further investigation. Another unknown question is the source of miracidia infecting the snails during flooding. During this season, most of the marshlands of the Yangtze River are submerged making it impossible for livestock to graze on the beaches. It is hypothesized that the miracidia may come from the exogenous faeces discharged by transportation boats in the Yangtze River. However, this also needs to be confirmed by further study.
People may raise a critical point that this system may not be able to get real-time infection information since the earliest detection time of the positive results is about 35-40 days after the sentinel mice are put into the field. However, on the other hand, it allowed us to carry on more targeted prevention and control of schistosomiasis along the Yangtze River, which means the control mode gradually changed from conventional large-scale approach to pinpoint control. This monitoring system made the implementation of schistosomiasis control more precise, which in turn further improved the cost-effectiveness of the conventional control process. Thus, small control inputs can generate great control effect. The future requires multivariate spatial analysis for the production of reliable risk maps showing high-risk areas and the scope of distribution. This should further guide the on-site emergency control work .
We would like to sincerely express our gratitude to the staff from Jiangsu Institute of Parasitic Diseases Control (Huang Yi-Xin, Wu Feng, Hua Wan-Quan, Zhao Song, Wang Xiao-Ting, Qian Chun-Yan, Xu Yan, Tang Jian-Xia, Li Wei, Zhang Jian-Feng) and the Center for Disease Control of Nanjing, Yangzhou, Zhenjiang, Changzhou and Taizhou cities. We sincerely thank Dr. Robert Bergquist for his tremendous contribution on paper editing. And we are also grateful to the anonymous referees for excellent comments. GJY is grateful to the “National nature science foundation”(81102173) and “Health promotion project, Outstanding person fund, Jiangsu provincial department of health(2011)”, respectively.
- Yang GJ, Utzinger J, Lv S, Qian YJ, Li SZ, Wang Q, Bergquist R, Vounatsou P, Li W, Yang K, Zhou XN: The Regional Network for Asian Schistosomiasis and Other Helminth Zoonoses (RNAS(+)) target diseases in face of climate change. Adv Parasitol. 2010, 73: 101-135.View ArticlePubMedGoogle Scholar
- Zhou XN, Yang GJ, Yang K, Wang XH, Hong QB, Sun LP, Malone JB, Kristensen TK, Bergquist NR, Utzinger J: Potential impact of climate change on schistosomiasis transmission in China. Am J Trop Med Hyg. 2008, 78: 188-194.PubMedGoogle Scholar
- Mao CP: Biology of Schistosome and Control of Schistosomiasis. 1990, Beijing: People's Health PressGoogle Scholar
- Yang GJ, Zhou XN, Sun LP, Wu F, Zhong B, Qiu DC, Utzinger J, Bradshaw CJ: Compensatory density feedback of Oncomelania hupensis populations in two different environmental settings in China. Parasit Vectors. 2011, 4: 133-10.1186/1756-3305-4-133.PubMed CentralView ArticlePubMedGoogle Scholar
- Bergquist R, Tanner M: Controlling schistosomiasis in Southeast Asia: a tale of two countries. Adv Parasitol. 2010, 72: 109-144.View ArticlePubMedGoogle Scholar
- Zhou XN, Bergquist R, Leonardo L, Yang GJ, Yang K, Sudomo M, Olveda R: Schistosomiasis japonica control and research needs. Adv Parasitol. 2010, 72: 145-178.View ArticlePubMedGoogle Scholar
- Hao Y, Yi DH, Zhang XF, Xiong JJ, Yuan WZ, Hu SJ, Wu XH, Zhu R, Guo JG, Huang XB: Assessment report on infection control of schistosomiasis in China, 2008. Chin J Schisto Cont. 2009, 21: 457-463.Google Scholar
- Huang CY, Zhao ZY, Ren GH, Wen SW, Li YS, Xiong MR: Trend analysis of endemic situation of schistosomiasis in Dongting Lake regions, Hunan Province from 2000 to 2009. Chin J Schisto Cont. 2010, 22: 247-250.Google Scholar
- Zhu R, Dang H, Zhang LJ, Li HZ, Zheng CJ, Wu XH, Guo JG: National surveillance of schistosomiasis in China, 2005-2008. Chin J Parasitol Parasit Dis. 2009, 21: 358-362.Google Scholar
- Zhang GH, Zhang SQ, Wang TP, Lv DB, Xiao X, Fang GR: Distribution of Schistosoma japonicum cercaria in Yangtz river during the flood period and it's effect on the human infection. J Trop Dis Parasitol. 2006, 4: 20-22.Google Scholar
- Zheng J, Qian K: The surveillance of schistosomiasis in China. Chin Pre Heal Care. 1988, 21: 23-25.Google Scholar
- Gu BL, Cao Q, Hang MT, Huang YX, Wang D, He SY: Six years post surveillance of 13 counties reached schistosomiasis transmission control in Jiangsu province. Chin J Schisto Cont. 1990, 2: 53-54.Google Scholar
- Fan XL, Tao HQ, Song CC, Hua DS, Chen ZP, Jiang YM, Lin ZC, Shen BR, Zhu DM, He SY: Studies on the monitoring schemes of schistosomiasis in controlled and basically controlled areas. Chin J Schisto Cont. 1990, 2: 6-9.Google Scholar
- Yuan H, Jiagang G, Bergquist R, Tanner M, Xianyi C, Huanzeng W: The 1992-1999 World Bank Schistosomiasis Research Initiative in China: outcome and perspectives. Parasitol Int. 2000, 49: 195-207. 10.1016/S1383-5769(00)00045-3.View ArticlePubMedGoogle Scholar
- Wang LY, Jiang QW, Liu JX, Zhao GM, Chen XY: National schistosomiasis endemic situation in 1999. Chin J Schisto Cont. 2000, 12: 321-323.Google Scholar
- Zhao GM, Zhao Q, Chen XY, Wang LY, Hao Y, He N, Wei JG, Jiang QW: The national surveillance of schistosomiasis between 2000 and 2001. Chin J Schisto Cont. 2003, 15: 19-52.Google Scholar
- Zhao F, Zhu R, Zhang LJ, Zhang ZJ, Li YP, He MZ, Zhou YB, Guo JG, Zhao GM, Jiang QW: Integrated detection and analysis on the clusters of schistosomiasis based on geographic information system. Chin J Epi. 2010, 31: 1272-1275.Google Scholar
- Malone JB, Yang GJ, Leonardo L, Zhou XN: Implementing a geospatial health data infrastructure for control of Asian schistosomiasis in the People's Republic of China and the Philippines. Adv Parasitol. 2010, 71-100.Google Scholar
- Zhou XN, Hu XS, Sun NS, Hong QB, Sun LP, Lu GN, Fuentes M, Maone JB: Application of geographic information systems on schistosomiasis surveillance-II. Predicting transmission intensity. Chin J Schisto Cont. 1999, 11: 66-69.Google Scholar
- Yang GJ, Vounatsou P, Zhou XN, Utzinger J, Tanner M: A review of geographic information system and remote sensing with applications to the epidemiology and control of schistosomiasis in China. Acta Trop. 2005, 96: 117-129. 10.1016/j.actatropica.2005.07.006.View ArticlePubMedGoogle Scholar
- Sun LP, Liang YS, Huang YX, Hong QB, Gao Y, Zhang LH, Gao Y, Xie CY: Evaluation on effect of infection control of schistosomiasis in Jiangsu Province. Chin J Schisto Cont. 2008, 20: 41-45.Google Scholar
- Sun LP, Liang YS, Wu HH, Tian ZX, Hong QB, Huang YX, Gao Y, Min J, Gao Y, Xie CY: Effect evaluation of comprehensive control for schistosomiasis in key villages of Jiangsu Province. Chin J Schisto Cont. 2009, 21: 285-289.Google Scholar
- Liang YS, Sun LP, Dai JR, Hong QB, Huang YX, Wang W, Zhang JF, Li HJ, Li W, Gao Y: Surveillance results in different months along the Yangtze River in Jiangsu Province. Chin J Parasitol Parasit Dis. 2009, 21: 363-367.Google Scholar
- MOH: Schistosomiasis Prevention Handbook. 1982, Shanghai: Science and Technique PressGoogle Scholar
- Chen MG, Feng Z: Schistosomiasis control in China. Parasitol Int. 1999, 48: 11-19. 10.1016/S1383-5769(99)00004-5.View ArticleGoogle Scholar
- Utzinger J, Zhou XN, Chen MG, Bergquist R: Conquering schistosomiasis in China: the long march. Acta Trop. 2005, 96: 69-96.View ArticlePubMedGoogle Scholar
- Lin DD, Wu XH, Jiang QW, Lin JJ, Zhou XN: Strategic emphasis for research development of schistosomiasis control in China. Chin J Schisto Cont. 2009, 21: 1-5.Google Scholar
- Zhou XN, Jiang QW, Sun LP, Wang TP, Hong QB, Zhao GM, Wen LY, Yin ZC, Wu XH, Lin DD: Schistosomiasis control and surveillance in China. Chin J Schisto Cont. 2005, 17: 161-165.Google Scholar
- Dang H, Zhu R, Guo JG: Surveillance of schistosomiasis in China from 2005 to 2007. Chin J Schisto Cont. 2008, 20: 241-244.Google Scholar
- Xie CY, Gao Y, Qiu L, Yin WG, Zhou W, Yang PC: Sentinel mice detection on schistosomiasis. Chin J Parasitol Parasit Dis. 2005, 23: 315-316.Google Scholar
- Yang WS, Yin GL, Xie FX, Duan YR, Yang J, Wu JZ: Detection of infested water of schisotosomiasis in Zhonghe village, Weishan county, Yunnan. Chin J Schisto Cont. 1996, 4: 187-Google Scholar
- Zhang SJ, Liu ZD, Hu LS, Xie ZW, Yuan SJ, Yan JZ, Zhong JH, Hu GH, Zhou SY, Sun YS, Qiu YX: Studies on epidemiological factors and regular partterns of schistosomiasis in Boyang Lake region. Chin J Schisto Cont. 1990, 2: 17-23.Google Scholar
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