- Open Access
Development and validation of IIKC: an interactive identification key for Culicoides (Diptera: Ceratopogonidae) females from the Western Palaearctic region
Parasites & Vectorsvolume 5, Article number: 137 (2012)
Background and methods
The appearance of bluetongue virus (BTV) in 2006 within northern Europe exposed a lack of expertise and resources available across this region to enable the accurate morphological identification of species of Culicoides Latreille biting midges, some of which are the major vectors of this pathogen. This work aims to organise extant Culicoides taxonomic knowledge into a database and to produce an interactive identification key for females of Culicoides in the Western Palaearctic (IIKC: Interactive identification key for Culicoides). We then validated IIKC using a trial carried out by six entomologists based in this region with variable degrees of experience in identifying Culicoides.
The current version of the key includes 98 Culicoides species with 10 morphological variants, 61 descriptors and 837 pictures and schemes. Validation was carried out by six entomologists as a blind trial with two users allocated to three classes of expertise (beginner, intermediate and advanced). Slides were identified using a median of seven steps and seven minutes and user confidence in the identification varied from 60% for failed identifications to a maximum of 80% for successful ones. By user class, the beginner group successfully identified 44.6% of slides, the intermediate 56.8% and the advanced 74.3%.
Structured as a multi-entry key, IIKC is a powerful database for the morphological identification of female Culicoides from the Western Palaearctic region. First developed for use as an interactive identification key, it was revealed to be a powerful back-up tool for training new taxonomists and to maintain expertise level. The development of tools for arthropod involvement in pathogen transmission will allow clearer insights into the ecology and dynamics of Culicoides and in turn assist in understanding arbovirus epidemiology.
During the last decade, the decline of fundamental entomological taxonomic expertise has become an increasing concern worldwide and has impacted directly upon disciplines as diverse as biodiversity conservation, medical and veterinary entomology[2, 3] and pest management. The correct classification of subject species is a vital prerequisite to any biological study and is a primary requirement for comparability across studies. Despite this, morphological taxonomy, which is by far the most commonly used means of identification used by biologists worldwide, receives relatively little financial support.
Ideally, identification of a biological specimen can be conducted using direct comparison with existing named specimens, including the original type. This comparative approach is feasible only when type locality is known accurately, the original specimen has been suitably preserved, and species description written in easy-access articles. While this is possible in larger institutions with a long track record of experimentation on a specific taxon, it is more common for the specimen to be compared to written descriptions and whatever identifying material (e.g. photographs, diagrams etc), is available through previously published work. The power of identification of groups of related organisms through the use of contrasting statements concerning morphological characters, also known as identification keys, was first realised by Lamarck (1778).
The development of electronic communications has revolutionised taxonomy worldwide, initially through facilitating contact between workers worldwide and more recently by allowing the open-access publication of taxonomic data. In addition, a large number of interactive keys allowing accurate identification of vector species and groups are increasingly available, either by downloading or directly through websites (e.g. Phlebotomine sandflies key tsetse flies and mosquitoes[7, 8]). These not only allow direct sharing of information, but also provide a powerful training tool where specialised expertise is otherwise reliant upon single individuals.
The recent unprecedented bluetongue virus (BTV) outbreaks in Western Europe illustrate how a relatively neglected arthropod vector group can rapidly increase in interest. BTV causes bluetongue (BT), a disease that affects wild and domestic ruminants, and the virus is biologically transmitted by various species of Culicoides Latreille biting midges (Diptera: Ceratopogonidae). At the time of introduction of BTV in 2006 to much of Western Europe, the number of groups working on Culicoides in Europe was small. Following the incursion, there was a substantial need to rapidly train workers in Culicoides taxonomy and this was in part accomplished through the use of online resources (e.g.http://www.culicoides.net) and direct training by the limited number of experts available. It was clear, however, that improvements could be made to this system from the following observations: (1) many workers had difficulty identifying the diversity of Culicoides present in their samples (particularly those species that did not fall within what were perceived to be the main vector groups), (2) many lacked either appropriate identification tools, or did not know where to find them (3) there was a lack of continuity and successive planning in preserving skills in taxonomy within countries, preventing the building of local expertise in Culicoides taxonomy.
Biological material, illustrations and choice of descriptors
Taxonomic information was collated from 98 slide-mounted Culicoides ( Additional file1). Twenty of these species were characterised from types preserved in the Callot and Kremer collection (Strasbourg, France). Data on C. paradisionensis was obtained from the type specimen in the Delécolle collection (Strasbourg, France), whereas the 77 other species were studied from specimens kept in the collection at IPPTS (Strasbourg, France). To ensure the reliability of the key, uncertainty due to intraspecific variation in morphology was avoided by coding some descriptors as polymorphic to ensure users did not discard the species erroneously. For ten species (noted with an asterisk in Additional file1), the presence of significant morphological variation led us to create a second entity of these species called a variation.
Morphological characters were image-captured using a Zeiss® microscope equipped with a Motic® camera, and were processed with the Gimp© editor version 2.6.2, (Free Software Foundation, Boston, USA). The list of morphological characters (Table 1) and state of characters were chosen through discussion with international experts at a meeting on Culicoides taxonomy in Strasbourg in 2009 (http://medreonet.cirad.fr/news/2009_taxonomy). A total of 73 taxa were characterised with 434 images (5.9 pictures/taxon) and 71 additional diagrams were also produced. Six rare taxa were not illustrated because of the poor quality of the specimens available. Among the 61 descriptors used, 60 were morphological characters (27 wing, 14 abdominal, 16 head and 3 leg characters) and one referred to the known geographical distribution (Table 1). The geographical descriptor was based on publications and included the 16 countries gathered around a European project (http://medreonet.cirad.fr/): Algeria, Belgium, Denmark, France, Germany, Greece, Italy, Morocco, Netherlands, Portugal, Spain, Sweden, Switzerland, Tunisia, Turkey and the United Kingdom. The graphical user interface is illustrated as a screenshot (Figure 1). The middle section of the interface was dedicated to definitions and images of both descriptors (on the left part) and taxa (on the right part). As a quick start guide, notices on “How to install” and “How to identify” were added.
Database management system
Xper2 version 2.0 was selected to edit and to manage the morphological database and to create the interactive key. It does not require advanced programming and can be freely downloaded athttp://lis-upmc.snv.jussieu.fr/lis/?q=ressources/logiciels/xper2. Xper² is a versatile software for editing, managing, storing and providing for on-line publishing of taxonomic knowledge. Several tools are available in order to facilitate the daily work of its users: the checkbase function prevents inconsistencies, the summary function can provide an overview of the whole knowledge base and items are easily compared within a matrix. In addition, Xper2 allows the use of operators to take into account the treatment of polymorphism or uncertainty. The descriptors can be sorted according to their discriminant power using three indexes: one is unique to the software, Xper² original sort, and two are well-known mathematical indexes, the Sokal and Michener sort, and the Jaccard sort.
IIKC was validated by 6 users with different levels of expertise in Culicoides identification. Two were beginners on Culicoides taxonomy, defined as possessing little experience with identification keys in general (users 1 and 2); two were defined as of intermediate skill, with experience with mosquitoes and tick taxonomy, but none with Culicoides (user 3 and 4); and two were defined as advanced users with expertise on Culicoides taxonomy and identification keys (user 5 and 6). A total of 37 slide-mounted female Culicoides representing 34 species morphologically confirmed by two experts, were sent without identifying labels to users. Specimens were recorded with a reference number and the trapping location.
A questionnaire was sent to the users to record the final species identifications, the time required for identification and the level of confidence the user attached to each identification. To begin the identification process, users activated the Xper original sort and then freely selected the descriptors among the list sorted in a decreasing order of discriminant power, i.e. from the descriptors that will best discriminate the taxa to the least. Identification slide orders were randomly selected for each user. After each specimen identification, users saved the identification pathway history (automatically generated by the Xper2 software) with the state of characters selected. To avoid heterogeneity in identification effort, users were recommended to complete only one identification process per specimen. Each original step was checked afterwards to see whether each morphological state chosen by the user discarded the correct taxon or not. The selection of a morphological state was considered as an error if the correct taxon was discarded and as a success if not. Each morphological selection of the step n was checked independently of the results of the step n-1 meaning a success due to a good morphological observation could be possible at the step n even if an error occurred at n-1 discarding the correct taxon. A step was considered as an observation from which success and error were computed, if the step discarded at least one taxon. Each of the 222 identification pathway histories generated by the six users was then checked to compute the quality of user observations. An observation (step) was computed as error if the selected state discarded the correct taxa and as success when the correct taxa remained in the taxa list.
Data from the validation step was analyzed with a factorial component analysis using the ade4 package of R software. The statistical tests were computed with R software. Differences of success frequency between users and between the user classes were investigated by a chi-squared test. Normality of dataset and subsets were assessed with the Shapiro-Wilks test. The mean differences of non-normal data were explored using the Kruskal-Wallis test. In case of significance of the latest, kruskalmc function of the pgirmess R package and the Wilcoxon test were used to investigate multiple comparisons between classes and within two classes.
Database contents and structure
IIKC database structure was based on descriptor dependency, with four hierarchical levels (Figure 2). All identifications started with a choice of 23 descriptors (level 1), 28 on level 2, 9 on level 3 and only 1 on level 4. Descriptors for level 1 are not inter-related meaning that selection between each of them is possible (Figure 2). Logical dependencies then determine the availability pathway of descriptors between levels 2-4 by removing redundant descriptors following the selection of particular characters.
As expected, identification pathways vary according to the user of the programme. As an illustration of this, a comparison of the selection process by two users to identify correctly C. newsteadi was documented (Figure 3) and compared with the optimised pathway following the “Xper original sort”. The number of steps, characters used and the final descriptors allowing discrimination of C. newsteadi were different. Comparing the first step of these three pathways, the optimised one discarded 62% of taxa compared to respectively 12% and 28% for the intermediate and advanced users.
Analysis of IIKC validation
A factorial component analysis between the different variables (slide order, identification time, confidence percentage and number of descriptors) was performed (data not shown). Projections of either slides or users to the factorial axis did not reveal any pattern. Identification data (identification time, success or failure to identify correctly the specimen, number of descriptors used) for each user was then individually analysed.
Successful identification took a median of seven steps (inter-quartile range of 3) and seven minutes (inter-quartile range of 5). Identification success rate varied according to the species concerned (Table 2). Success rates differed significantly between users (chi-squared test, p = 0.0033) and between levels of experience (chi-squared test, p = 0.0011) and ranged from 35.1% to 81.1%. By level of experience, the beginners successfully identified 44.6% of slides, the intermediates 56.8% and the advanced 74.3%. Each specimen, however, was correctly identified at least once within the group and four specimens, (C. nubeculosus, C. parroti, C. saevus and C. semimaculatus) were correctly identified by all users (Table 2). For all three user categories, median confidence was 60% for failed identifications and 80% for successfully identified specimens.
For users, successful identifications were achieved in an average of 6.6 steps, with a minimum of two steps (for C. saevus) and a maximum of 15 steps (for C. punctatus). No significant difference was observed between the number of descriptors used when identification failed, succeeded or both, either between users or user’s class (Kruskal-Wallis test, p > 0.05). For all users, the identification time was significantly higher when identification failed than when identification succeeded (one-sided Wilcoxon test, p = 0.0093). No significant differences were noted, however, either between users or within the user’s class (all Kruskal-Wallis tests, p > 0.05) although complete data was not available for the beginner class.
Eight out of 61 descriptors were not used during the validation (wing characters: W05, W06, W09, W11, W18, W20, W23 and head character: H11). Users selected a total of 1,397 character states for 53 descriptors of which seven descriptors represented 50% of the descriptors used, namely by decreasing order: W01, A01, H02, A02, H15, H06 and H07 (Figure 4). Most successful descriptors included the use of A01 and H02, which led to error in less than 5% of cases and W01 and H06, which led to error in less than 10%. Similarly, the sclerotized ring (A02), sensilla distribution (H15) and sensory pits (H07) were used with 12, 11 and 11% of error respectively. In all, 36 descriptors represented 95% of use of the key. Each user demonstrated a particular pattern of preference for use of descriptor groups (Figure 5). As an example only one user made an initial sort according to the origin of the specimen. The beginners and the intermediate users also utilised very different patterns of descriptor use. The advanced group had a more similar pattern giving priority to observation of the head followed by the abdomen and the wings and additionally avoided observing legs and using the geographical descriptor.
This study has produced and validated IIKC, the first open-access electronic key for Culicoides to be developed worldwide and has demonstrated some of the advantages and disadvantages in providing taxonomic information to a range of different users using e-tools. The validation was carried out with the aim of investigating the impact that the key could have upon users ranging from beginners with no experience of either electronic keys or biting midge identification, to those carrying out Culicoides identification daily, but whom were trained in a different laboratory and country (in this case the United Kingdom). This was used to reflect the scenario that in the event of the incursion of a Culicoides-borne pathogen, staff with highly diverse levels of experience are expected to contribute to identification of Culicoides across a wide geographical range. In addition, rather than just including Culicoides species that were expected, a priori, to act as vectors, the validation included a challenging and realistically wide range of specimens that might be collected at light in the region (allowing a fuller understanding of species diversity)[9, 17–20].
The validation results provided valuable information regarding the likely accuracy of surveys conducted by users of different levels of expertise and also highlighted improvements that could be made to IIKC, allowing an assessment of the degree to which specialist coaching would still be required in an outbreak situation. The relatively low success of the advanced users (74.3%) could be explained by two phenomena. First, we cannot underestimate the fact that all users may be puzzled when they discovered the key for the first time during the trial. Indeed, independently of the difficulty of species identification, the random order of specimens during the identification process demonstrated that half of the errors occurred for the first 14 specimens. This observation was confirmed by user feedback, which estimated that around 10 identification processes were necessary to feel comfortable with the software interface. The absence of errors occurring for the last seven specimens would indicate a tendency to reach 100% success rate for the advanced users. Secondly, the advanced users have realized afterwards that their observations of the subjective characters, sometimes did not match with their final and confirmed diagnosis. Their observations of the non-corresponding subjective characters were computed as errors in this analysis, and consequently downgraded their success rates. Such subjective characters e.g. the neck of the spermathecae or the shape of palpus, have revealed a need to update data eventually by coding them as polymorphic. Some species are clearly easier to identify, even for beginners, due to distinctive features, which are simple to observe, such as the swollen duct of the unique spermatheca of C. nubeculosus. Apart from such unique features, the species with wings that have well marked patterns were less problematic for users. On the contrary, the species causing the most difficulty were those with only two small and faint spots on the wings such as C. paradisionensis. Without experience regarding the intraspecific range of variations on Culicoides wing patterns, the difficulty in determining such a wing as with or without spots is real. To prevent errors due to subjective state of characters, database updates would be focused on making them clearer. Similarly, in case of a doubt in choosing the right character, we will enhance the functionality of Xper to allow users selecting more than one state of character. Primarily focused on microscopic characters, other features such as the coloration of the dorsum of the thorax e.g. useful for C. flavipulicaris or C. clastrieri, the scutum pattern e.g. C. nubeculosus or C. riethi, observable on specimens in alcohol would be added in the future to allow users to make a first sort before confirmation by slide-mounting.
The number and quality of images available in the IIKC guides users through the identification process, allowing them to assess their confidence in the result produced. Its flexibility through the use of a multi-entry system is also demonstrated by the fact that different users can use two different pathways to identify C. newsteadi, depending upon the characters they feel confident in applying (Figure 5). This system also has an additional advantage in allowing avoidance of descriptors that correspond to a damaged/missed anatomical part in the specimen. With experience, the user behaviour seems to concentrate upon characters of the head and the abdomen more than on the wings and very few are observed on the legs. Advanced users additionally never used the geographical character, probably concluding through their experience that most species are widely distributed.
Beyond the 36 descriptors that represented 95% of the whole observations, 8 descriptors were never used for several reasons. The distribution of the short sensilla trichodea (H11) was probably not used because of the difficulty to observe them without experience. Additionally, characters W05 and W11 were special features specific to C. caucoliberensis and C. simulator respectively, which were absent from the validation trial. The other five descriptors - W06, W09, W18, W20 and W23 – were not special features discriminant of species. In these cases the position on the list could have been a determining factor in their use. At present it is not possible to add weights to the descriptors either in terms of ease of use or specificity, however, this is planned in forthcoming developments and will take into account the feedback of those involved in the trial.
Technically, the software itself is relatively straightforward to operate and assists accurate identification in several ways. Uptake of the various tools provided within the programme is of interest in approving the acceptability to different user groups. To assist in identification, the software allows three options: Option 1 allows managing uncertainty by using logical operators (like AND, XOR, NOT) to select several choices within the key. Even though this could be useful on occasion for difficult or subjective characters (like sensilla distribution or the palpus shape), none of the users used this function during the validation although this may be through a lack of awareness or confidence. The second option is to define a mismatch threshold when performing identifications. Each value for this option was not evaluated and no recommendation could be made. Nevertheless, an observed effect to increase the mismatch threshold is to increase the number of steps to identify. This is balanced by the fact that the validation protocol revealed that identifications requiring a lot of steps often lead to a higher number of errors. The last option assisting in identification is to compare the selected taxa by producing a matrix summarizing descriptions, with an easy to read colour-code indicating whether a character is discriminating, partially discriminating or not discriminating. This latter option could be used to improve the user knowledge and his confidence by checking which characters are discriminating among the selected taxa.
To date, all available identification tools for Culicoides are based upon single-access keys and are in specialist journals or PhD theses, which are often not easily available to new users. IIKC sits between very general databases that act as a repository for a wide variety of information concerning Culicoides biology (e.g.http://www.culicoides.net orhttp://bluetongue.cirad.fr/) and published keys, and will allow at least basic competence to be developed by users. While the identifications made by beginners will still require secondary confirmation by experts (and these confirmations in themselves are prone to subjective biases), the provision of the key online and with access to other workers will significantly improve the consistency of Culicoides identification in Northern Europe. Taxonomy as a discipline has advanced substantially through internet-based resources as it is reliant upon both detailed description and high quality images. While not replacing the specialist’s role in training new taxonomists, IIKC provides a complementary mechanism as a back-up tool for experts. According to the trial results, IIKC will be improved by weighting descriptors that are easy to observe, by evaluating the mismatch thresholds for beginners, by developing definitions and images for difficult descriptors and by adding illustrations and information of particular features of species. A scientific committee will be organised to validate updates, to discuss new species or synonymies and to evaluate new systematic or taxonomic changes.
IIKC is available in a cd-rom format upon request from the authors or can be downloaded from the following websitehttp://www.iikculicoides.net.
IIKC, an Interactive Identification Key for females of the species of Culicoides of the West Palaearctic region, is a multi-entry key providing taxonomic information for 98 species and 10 variants with 837 photographic images and illustrations. In addition to the key, users can browse the database including morphological data for 60 characters, synonymies and geographical distribution among 14 countries. Validated by six users with a various range of experience, IIKC appears to be straightforward to use. In addition to the key, the huge amount of taxonomic information available acts a back-up source for the e-taxonomy of the genus Culicoides. The development and the free sharing between beginners and experts of the e-taxonomy such as IIKC for Culicoides and more generally for arthropods involved in pathogen transmission will unlock the taxonomic knowledge to identify species and therefore will give better insights into the ecology and dynamics of these groups, helping to standardise vector surveillance strategies across countries.
Agosti D: Biodiversity data are out of local taxonomists' reach. Nature. 2006, 439: 392-
Oliver JH: Crisis in biosystematics of arthropods. Science. 1988, 240: 967-10.1126/science.3368789.
Cuisance D, Rioux JA: Current status of medical and veterinary entomology in France: endangered discipline or promising science?. Comp Immunol Microb. 2004, 27: 377-392. 10.1016/j.cimid.2004.03.007.
Song H: Grasshopper Systematics: Past, Present and Future. J Orthopt Res. 2010, 19: 57-68. 10.1665/034.019.0112.
Vignes-Lebbe R, Gallut C: Computer Aided Identification of Phlebotomine sandflies of Americas (CIPA). 1997, Université Pierre et Marie Curie, Paris,http://lis-upmc.snv.jussieu.fr/xper2/infosXper2Bases/,
Brunhes J, Cuisance D, Geoffroy B, Hervy J: Les glossines ou mouches tsé-tsé (réédition). (Cd-Rom). 2009, Montpellier: IRD
Schaffner F, Angel G, Geoffroy B, Hervy J, Rhaiem A, Brunhes J: The mosquitoes of Europe. An identification and training software. (Cd-Rom). 2001, IRD, Montpellier
Hervy JP, Le Goff G, Geoffroy B, Hervé JP, Manga L, Brunhes J: Les anophèles de la région afrotropicale. (Cd-Rom). 1998, IRD, Montpellier
Carpenter S, Wilson A, Mellor PS: Culicoides and the emergence of bluetongue virus in northern Europe. Trends Microbiol. 2009, 17: 172-178. 10.1016/j.tim.2009.01.001.
Cêtre-Sossah C: Strengthening cooperation on vectorborne diseases in Europe. Vet Rec. 2010, 166: 386-387.
Campbell JA, Pelham-Clinton EC: A taxonomic review of the british species of Culicoides Latreille (Diptera: Ceratopogonidae). Proc R Soc Edinburgh. 1960, 68: 181-302.
Kremer M: Contribution à l'étude du genre Culicoides Latreille particulièrement en France. 1965, Encyclop Ent Serie A, Paris
Delécolle JC: Nouvelle contribution à l'étude systématique et iconographique des espèces du genreCulicoides(Diptera : Ceratopogonidae) du Nord-Est de la France.PhD thesis. 1985, Université Louis Pasteur de Strasbourg, UFR sciences de la vie et de la terre
Mathieu B, Cêtre-Sossah C, Garros C, Chavernac D, Balenghien T, Vignes-Lebbe R, Ung V, Candolfi E, Delécolle JC: IIKC: An Interactive Identification Key for female Culicoides (Diptera: Ceratopogonidae) from the West Palearctic region. Proceedings of the international congress Tools for Identifying Biodiversity: Progress and Problems: 20-22 September 2010; Paris. Edited by: Nimis PL, Vignes-Lebbe R. 2010, , , 201-205.
Ung V, Dubus G, Zaragueta-Bagils R, Vignes-Lebbe R: Xper2: introducing e-taxonomy. Bioinformatics. 2010, 26: 703-704. 10.1093/bioinformatics/btp715.
R Development Core Team: R: A Language and Environment for Statistical Computing. 2010, R Foundation for Statistical Computing, Vienna, Austria,http://wwwR-projectorg,
Patakakis MJ, Papazahariadou M, Wilson A, Mellor PS, Frydas S, Papadopoulos O: Distribution of Culicoides in Greece. J Vector Ecol. 2009, 34: 243-251.
Venail R, Balenghien T, Guis H, Tran A, Baldet T, Setier-Rio ML, Delécolle JC, Mathieu B, Martinez D, Languille J, Garros C: Assessing diversity and abundance of vector populations at a national scale: example of Culicoides surveillance in France after bluetongue virus emergence. Parasitology Research Monographs. Edited by: Mehlhorn H. 2012, Springer-Verlag, Berlin Heidelberg, 77-102. [Mehlhorn H (Series Editor): Arthropods as Vectors of Emerging Diseases, vol 3.]
Romon P, Higuera M, Delecolle JC, Baldet T, Aduriz G, Goldarazena A: Phenology and attraction of potential Culicoides vectors of bluetongue virus in Basque Country (northern Spain). Vet Parasitol. 2012, 186: 415-424. 10.1016/j.vetpar.2011.11.023.
Viennet E, Garros C, Lancelot R, Allene X, Gardes L, Rakotoarivony I, Crochet D, Delecolle JC, Moulia C, Baldet T, Balenghien T: Assessment of vector/host contact: comparison of animal-baited traps and UV-light/suction trap for collecting Culicoides biting midges (Diptera: Ceratopogonidae), vectors of Orbiviruses. Parasit Vectors. 2011, 4: 119-10.1186/1756-3305-4-119.
Godfray HC: Challenges for taxonomy. Nature. 2002, 417: 17-19. 10.1038/417017a.
Borkent A: World species of biting midges (Diptera: Ceratopogonidae). [http://www.inhs.illinois.edu/research/FLYTREE/CeratopogonidaeCatalog.pdf].,
The development of IIKC was started in the framework of a surveillance network of reoviruses, bluetongue and African horse sickness in Mediterranean basin and Europe (acronym MedReoNet) Call FP6-2005-SSP-5-A Area 1.4. A part of this work was funded by the French Ministry of Agriculture. The authors would like to acknowledge all the partners of the MedReoNet Workpackage 2 and especially R. Meiswinkel, F. Schaffner, M. Miranda. A special thanks to R. Venail, J.B. Ferre, X. Allène, I. Rakotoarivony, L. Gardès, K. Huber, J. Barber and E. Denison for testing the beta version. The authors would like to express their sincere thanks to Art Borkent and the anonymous reviewer to provide critical and useful comments to improve the manuscript.
The authors declare that they have no competing interests.
TB, BM & JCD initiated the study; BM & DC drafted the database and RVL & VU contributed to the final database structure; BM & JCD collected the morphological data; JCD made the drawings; BM took the pictures and designed the scheme to define characters; SC, MLSR, EC, TB & CG organized the trial in their laboratory; TB & BM carried out the data analysis and interpretation; BM, CG, CCS & SC wrote the manuscript; All authors read and approved the final version of the manuscript.