Phenetic and genetic structure of tsetse fly populations (Glossina palpalis palpalis) in southern Ivory Coast
Parasites & Vectors volume 5, Article number: 153 (2012)
Sleeping sickness, transmitted by G. p. palpalis, is known to be present in the Ivory Coast. G. p. palpalis has recently been reported to occur in several places within the town of Abidjan, including: (i) the Banco forest, (ii) the Abobo Adjamé University campus and (iii) the zoological park. Could these three places be treated sequentially, as separate tsetse populations, or should they be taken as one area comprising a single, panmictic population?
The amount of gene flow between these places provides strategic information for vector control. It was estimated by the use of both microsatellite DNA and morphometric markers. The idea was to assess the interest of the faster and much less expensive morphometric approach in providing relevant information about population structure. Thus, to detect possible lack of insect exchange between these neighbouring areas of Abidjan, we used both genetic (microsatellite DNA) and phenetic (geometric morphometrics) markers on the same specimens.
Using these same markers, we also compared these samples with specimens from a more distant area of south Ivory Coast, the region of Aniassué (186 km north from Abidjan).
Neither genetic nor phenetic markers detected significant differentiation between the three Abidjan G. p. palpalis samples. Thus, the null hypothesis of a single panmictic population within the city of Abidjan could not be rejected, suggesting the control strategy should not consider them separately. The markers were also in agreement when comparing G. p. palpalis from Abidjan with those of Aniassué, showing significant divergence between the two sites.
Both markers suggested that a successful control of tsetse in Abidjan would require the three Abidjan sites to be considered together, either by deploying control measures simultaneously in all three sites, or by a continuous progression of interventions following for instance the "rolling carpet" principle. To compare the geometry of wing venation of tsetse flies is a cheap and fast technique. Agreement with the microsatellite approach highlights its potential for rapid assessment of population structure.
Tsetse flies (Diptera: Glossinidae) are the main vectors of trypanosomes (Kinetoplastida: Trypanosomatidae), which cause human and animal trypanosomiases in subsaharan Africa. These diseases have a considerable impact on public health and economic development, although there are recent signs of a decline in incidence of the human disease following WHO-supported interventions based on case detection and treatment[2–4]. Vector control is an important complement to case detection and treatment, because reducing vector density can rapidly halt human trypanosomiasis transmission[5, 6]. Vector control also remains the only strategy able to protect humans from acquiring a new infection.
Tsetse populations may be reduced using a variety of techniques, including insecticide impregnated traps and targets, live-baits, sequential aerial spraying, and sterile male release[8–13]. However, in many cases when the control efforts have been stopped, the tsetse populations tend to recover due to flies surviving the initial interventions, or migrant flies coming from untreated regions, or both.
This has fueled debate as to whether in some instances "eradication" (defined by FAO as the creation of a tsetse free zone) may be more cost-effective than "suppression" where tsetse densities are reduced to a level minimizing the risk of disease transmission. Decisions on eradication or suppression strategies will be facilitated when the population structure within the target region, in particular the degree of genetic isolation of the target population from adjacent populations is clearly understood. For isolated populations, eradication may be the most cost-effective strategy, as reported for Glossina austeni Newstead on Unguja Island, Zanzibar. But for most mainland populations of tsetse, the geographical limits of target tsetse populations are less easily defined. Application of techniques that can detect population isolation such as molecular or morphometric markers can guide decisions on the choice of control strategies[15–17]. Human and animal trypanosomiasis transmitted by G. p. palpalis are known to be present in Ivory Coast[4, 18] and G. p. palpalis has been reported to occur within the city of Abidjan[19, 20]. Due to its potential danger as a vector of human and animal trypanosomiasis, the Ivorian authorities now seek to control these tsetse flies in the affected area of Abidjan, which includes the Banco forest, the University of Abobo Adjamé and the zoological park. Tsetse have been found to be present in low to high densities in these 3 sites, and were found infected by various trypanosome species.
To detect possible evidence of isolation between G. p. palpalis populations in the three affected areas within Abidjan, we used both genetic (microsatellite DNA) and phenetic (geometric morphometrics) markers on the same specimens, and compared these populations to G. p. palpalis populations from another area of southern Ivory Coast in the region of Aniassué. The idea was to assess the interest of the faster and much less expensive morphometric approach in providing relevant information about population structure.
The expected outcome of this study was to help the national control program to decide which is the best strategy of vector control in the town of Abidjan: can these three localities be treated sequentially (i.e. are the tsetse populations isolated between the three sites), or should they be taken as one area comprising a single, panmictic population?
Results and discussion
Microsatellite DNA markers
Within sample analyses
For the total sample (n = 141) of genotyped tsetse, the seven microsatellite loci displayed 17 (Pgp1), 17 (PgP13), 14 (PgP24), 25 (B104), 19 (B110), 7 (C102), and 9 (GPCAG) alleles, respectively. The mean number of alleles was 9.71 (Banco), 11 (University) and 10.85 (Zoo) in Abidjan, and 10.00 in Aniassué. Mean observed heterozygosities were 0.68, 0.76 and 0.77 for Banco, University and Zoo, respectively, and 0.70 in Aniassué (no significant difference).
Overall Fis values were 0.12, 0.09 and 0.05 for Banco, University, and Zoo, significant at p<0.0001, p<0.001, and p<0.05 respectively. In Aniassué, Fis was 0.15, p<0.0001. The heterozygote deficit was mainly due to two loci (PgP1 and B110) for the three populations of Abidjan (Figure1).
This suggested locus had specific technical problems (e.g. null alleles or short allele dominance), because when these loci were removed from the analysis, Fis values dropped to non-significant values (0.04, 0.00 and 0.03, respectively). Hence the null hypothesis of panmixia in Abidjan could not be rejected. In Aniassué, Fis on these 5 loci was 0.18 (p<0.0001), indicating consistant heterozygote deficiency. The heterozygote deficiency found in Aniassué confirmed earlier observations on G. p. palpalis in the forested areas of Ivory Coast, which attributed such deficiency to a combination of null alleles and genetic structuring at local scale due to Wahlund effects.
Genetic differentiation between samples
The mean Fst value for the 5 loci among the four populations was estimated at θ = 0.017 (CI95: 0.011 <θ< 0.023), p<0.0001. For the Abidjan samples it was θ = 0.007 (CI95: 0.00150 <θ< 0.01184) and was not significant, meaning that most of the differentiation was due to differences between Aniassué and Abidjan. Looking at paired Fst values between sites (Table1) confirmed this, since the highest (and significant) values always included Aniassué. Within Abidjan, there was a slight but non-significant trend for the population of Banco to diverge (Fst=0.01, p<0.05) from those of University and Zoo, whereas the latter two were genetically similar.
Size: centroid size
The specimens from Aniassué were significantly smaller compared to those from Abidjan, whereas within Abidjan there was no significant size difference between flies from the three sites (Figure2).
The first two discriminant factors derived from the shape variables showed that the polygon representing the Aniassué population tended to separate from the Abidjan sites (Figure3). The reclassification tree, based on all three of the discriminant factors, clearly separated the Aniassué sample from those from Abidjan (Figure4).
The Mahalanobis distances between the Abidjan samples (Table1) were not significantly different, indicating an absence of shape differentiation, while the Mahalanobis distances from Aniassué were significantly larger (p<0.007) (Table1).
The validated reclassification scores confirmed this pattern, since Aniassué had the highest score (86%). However, in spite of the lack of significant differentiation within Abidjan, the reclassification score obtained for Banco (77%) was much higher than for the University (37%) and Zoo (33%), suggesting a relatively higher level of shape divergence in the Banco forest.
Correlation between metric and genetic distances was high. Regression of the Mahalanobis distances on the genetic distances indicated that 79% of the morphometric variation could be explained by the genetic variation (Figure5).
Genetic and morphometric differentiation
From an epidemiological point of view, our study aimed at knowing whether tsetse populations from three sites in Abidjan could be considered to be isolated from each other. Such information is relevant for designing an adequate tsetse control strategy. For example, an insecticide application could be sequential in case of separation between sites, working on each site separately without risk of reinvasion to the next, or it should simultaneously cover all three sites if no evidence for separation is found.
We used a population genetics approach to analyse possible separation between the three Abidjan populations, comparing genetic and phenetic markers. Thus, the study also tested the potential of geometric morphometrics as a possible surrogate for molecular markers.
Both the phenetic (geometric morphometrics) and genetic (microsatellite loci) markers showed no evidence for differentiation between G. p. palpalis from sites within Abidjan, but both markers agreed in showing strong differentiation between individuals from Aniassué and those from Abidjan.
At the scale of Abidjan, our data showed that males from the three sites showed no genetic differentiation, and accordingly had similar metric properties (size and shape).
The microsatellite markers did not show any significant departure from the null hypothesis of panmixia, i.e. we did not observe any genetic differentiation between the 3 populations within Abidjan. There was however a slight, non significant trend for the population of Banco to diverge from the two others. A possible explanation is then a slow, on-going process for this population of Banco to have less genetic exchanges with the two others, due to urbanization which restricts tsetse movements. It may be possible, as observed in other studies in Burkina Faso, that the molecular markers used are not sensitive enough to detect it, since this is a recent, on-going phenomenon whereas what the molecular markers show is the result of a genetic history over several generations. This lack of sensitivity of molecular markers for recent genetic changes has already been observed in tsetse studies, and may be compensated by the use of morphometrics.
This idea is reflected by the much higher shape-based reclassification score obtained for Banco (77%), compared to the two other sites (37% and 33%). This indirect evidence for some morphometric specificity in the forest might be due to an environmental effect ("forest" versus "city"), although in tsetse most of the pre-imago development is relatively protected from external influences as tsetse larvae grow in the uterus of their mother during the three first stages, buffering morphometric variations against external influences.
Temperature and humidity do become influential factors at the time when pupae are in the soil. The effect has been studied for size (not shape), indicating that higher temperatures tend to result in smaller individuals, whereas increasing humidity tends to result in larger individuals. It has been shown that the size of G. p. palpalis in forested areas of Ivory Coast is governed by seasonal climatic effects. In Abidjan, no size difference was detected between sites, and given their proximity it seems likely that environmental factors acted uniformly on size.
Between Abidjan and Aniassué
By contrast, both molecular (microsatellite loci) and morphometric (centroid size and shape variables) data showed significant differences between tsetse from Abidjan and Aniassué. This was expected due to the geographic distance between the two sites (186 km), to the differences of biotopes, and to the fact that the tsetse belt in South Ivory Coast is discontinuous as a consequence of anthropic pressure on habitats.
The differences between G. p. palpalis from Abidjan and Aniassué also involved shape, which may reflect genetic variations[23, 27], especially when shape is allometry-free[28–30]. This was confirmed by differences found using microsatellite DNA markers. The parallel between phenetic and genetic markers applied to natural populations is not uncommon; for G. p. gambiensis, a similar parallel was observed in natural populations of different biotopes from West Africa, Guinea, Burkina Faso and Senegal. Here, 79% of the variance in Mahalanobis distance could be "explained" by genetic variation (compared to 50% in study by) study). This correlation does not imply a causal relationship, and could be attributed to both phenetic and genetic distances being related to geographical distances.
The heterozygote deficits found in Aniassué confirmed earlier observations on G. p. palpalis in the forested areas of Ivory Coast, which attributed such deficits to a combination of null alleles and genetic structuring at local scale due to Wahlund effects.
How can the knowledge of population structure help to choose a control strategy? Since microsatellite and morphometric markers did not show significant differentiation between tsetse from the three sites in Abidjan, there would appear to be no significant barrier to gene flow at this scale. From a control perspective, this means that intervention against tsetse in any one site is likely to face reinvasion from the other two. This is different from a similar study conducted on the Loos archipelago, Guinea, which showed that tsetse populations (G. palpalis gambiensis) were isolated from the mainland and structured according to the island[15, 34], which then allowed a sequential control strategy to be implemented[16, 35]. Successful control of tsetse in Abidjan however, would require all three sites to be considered together (Figure6), either by deploying control measures simultaneously in all three sites, or by a continuous progression of interventions - for example using barriers of impregnated traps and/or targets between sites (Figure7) following the "rolling carpet" principle.
In Abidjan, the three study sites were the Banco forest (Banco), the University of Abobo Adjamé (University) and the zoological park (Zoo). The Banco forest is in the north-western part of the city of Abidjan, at 5°N latitude and 4°W longitude. East of Banco are two small relicts of the forest which have now been substantially degraded by urbanisation: the Abobo Adjamé University and the zoo of Abidjan. These three sites, although geographically close (less than 500 meters between sites), are separated by roads and urbanisation (Figure8). For comparison, another study site was chosen near the town of Aniassué, about 186 km from Abidjan, in the Department of Abengourou, where G. p. palpalis occurs along the Comoé river. This region is characterized by forest degraded by wood cutting, and also by food crops (banana, cassava) and old cocoa plantations.
There is little temperature difference between Abidjan (from 24.2°C to 27.7°C) and Aniassué (from 24.3°C to 27.9°C), but relatively more variation in relative humidity (RH), which decreases from south (RH on average 90%) to north (RH between 60% and 70%). In both areas, there are two rainy and two dry seasons during a year.
Tsetse samples and microsatellite DNA markers
Tsetse flies were caught using Vavoua traps in April 2007 in Aniassué and in October 2007 in Abidjan. In total from Abidjan 111 individual tsetse were analysed using microsatellite DNA markers: Banco (25 females (F), 11 males (M)); University (21 F, 17 M) and Zoo (21 F, 16 M). From Aniassué, 30 individuals were analysed (15 F and 15 M). Seven microsatellite markers were used (preceded by "X" for X-linked loci): Pgp1, XPgp13, Pgp24, XB104, XB110, C102 (A. Robinson, FAO/IAEA, pers. com.) and GPCAG. The samples were processed for Polymerase Chain Reaction (PCR) and genotyping on a 4300 DNA Analysis System from LI-COR (Lincoln, NE) as described in.
Population genetics analyses on molecular markers
Wright's F-statistics, the parameters most widely used to describe population genetic structure, were initially defined for a three-level hierarchical population structure (individuals, sub-populations, and total). In such a structure, three fixation indices or F-statistics can be defined.
Fis is a measure of the inbreeding of individuals (hence I) resulting from non-random union of gametes within each sub-population (hence S).
Fst quantifies the differentiation between subpopulations in the total population (hence S and T) as a measure of the relatedness between individuals resulting from non-random distribution of individuals between sub-populations, relative to the total population.
Fit is a measure of the inbreeding of individuals resulting both from non-random union of gametes within sub-populations, and from population structuring (deviation from panmixia of all individuals of the total population, hence I and T).
These F-statistics were estimated by Weir and Cockerham's unbiased estimators f (for Fis), θ (for Fst) and F (for Fit). The significance of the F-statistics was tested by 1000 random permutations in each case. The significance of Fis was tested by randomizing alleles between individuals within sub-samples. The significance of Fst was tested by randomizing individuals among sub-samples.
Geometric morphometrics analyses
The tsetse specimens used for geometric morphometrics constituted a subsample of those on which the molecular analyses were done. Out of the 141 flies used for microsatellites, 55 had non-damaged wings allowing morphometric analyses. The analyses were conducted only on males, and focused on the right wing, which was generally the wing in best conditions. A total of 55 right wings of G. p. palpalis males (M) were used, i.e. 9 from Banco, 16 from University, 15 from Zoo and 15 from Aniassué.
Wings were dry-mounted between two microscope slides and scanned at 1800 ppp at dimensions of 0.90 x 0.50 cm, using a multifunction scanner HP Deskjet F 2180. From this picture, the coordinates of 10 landmarks (LM) defined by vein intersections were recorded for each wing, by the same person in the same order (Figure9). Repeatability was estimated at better than 80% (discussed elsewhere:).
The size variable was the isometric estimator known as centroid size (CS) derived from coordinate data and defined as the square root of the sum of the squared distances between the center of the configuration of landmarks, and each individual landmark. Statistical significance for size comparisons was estimated by 1,000 permutation tests with Bonferroni correction.
The 16 shape variables were the "partial warps" (PW). To circumvent the problem of small sample sizes relative to the large number of shape variables (16 PW), we used the first 6 principal components of the PW (relative warps, RW) as input for discriminant analyses, as these represented 84% of the total variation and had the highest discriminatory power.
Mahalanobis distances computed from these 6 RW were used to quantify shape divergence between groups (Figure4) and the statistical significance was estimated by 1000 permutation tests with Bonferroni correction.
Mahalanobis distances based re-classification scores were computed according to a validation procedure whereby each individual was assigned to its closest group without using that individual to help determine a group centre, although the computed shape variables did include that individual (Table2).
Collections of anatomical landmarks of the wings, general Procrustes analysis (GPA), multivariate and discriminant analyses, were performed using the CLIC package, freely available athttp://www.mpl.ird.fr/morphometrics/clic/index.html. PHYLIP software with "neighbor" module and NJPLOT were used to build the classification tree. The F-statistics from molecular data were estimated with Genetix and Fstat 126.96.36.199 (updated from). The overall G-test was used to estimate the significance of Fst with Fstat.
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We thank Prof. Mike Lehane for his kindly supporting the training of first author in geometric morphometrics. The following technicians contributed to this work: B. Coulibaly, F. Dofini, N’Dri Louis, K. Lohourignon and A. De marie Koffi. This study benefitted from the support of the LTTRN network, the EU INCO-DEV project N°031849 and the "Laboratoire mixte International sur les Maladies à Vecteurs" (LAMIVECT, Bobo-Dioulasso, Burkina Faso).
The authors declare that they have no competing interests.
Genetic techniques: SR, KA, LG, EKN'G, PS. Morphometric techniques: DK, HB-V, J-PD. Data analyses: DK, SR, PS, J-PD. Field collections: DK, MK, GA-Y. Text: DK, PS, J-PD, CJS. All authors read and approuved the final version of the manuscript.
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Kaba, D., Ravel, S., Acapovi-Yao, G. et al. Phenetic and genetic structure of tsetse fly populations (Glossina palpalis palpalis) in southern Ivory Coast. Parasites Vectors 5, 153 (2012). https://doi.org/10.1186/1756-3305-5-153