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Figure 1 | Parasites & Vectors

Figure 1

From: Monitoring and evaluation of lymphatic filariasis interventions: an improved PCR-based pool screening method for high throughput Wuchereria bancrofti detection using dried blood spots

Figure 1

Amplification and melting curves of LDR real-time PCR (qPCR): impact of the heating step: Blood samples with 1 mf (blue curves) and with 100 mf (red curves) were tested, The number of reaction cycles needed to detect a signal was lower when the lysate had been heated at 100°C before DNA purification (cross curves) than without this heating step (circle curves). The melt peak temperature was the same for each sample = 76.5°C.

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Parasites & Vectors

ISSN: 1756-3305

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