Monitoring and evaluation of lymphatic filariasis interventions: an improved PCR-based pool screening method for high throughput Wuchereria bancrofti detection using dried blood spots

Table 1 qPCR results depending on lysate volume processed and on microfilaria (mf) burden

mf per sample	Volume of lysate (μl)	Sample replicates	Ct mean	Ct range	Tm
1	600	3	29.8	[28.6-31.9]	76.5
1	100	4	31.9	[31.0-33.1]	76.5
100	600	2	21.6	[21.5-21.6]	76.5
100	100	2	23.6	[23.5-23.7]	76.5

ISSN: 1756-3305