Table 2 Ct values depending on lysate volume and lysate number per pool
Sample volume (μl) | Pool size | Pools processed | Ct mean | Range | Tm |
|---|---|---|---|---|---|
100 | 1 | 4 | 31.9 | [31–33.1] | 76.5 |
5 | 4 | 31.4 | [30.3-32.2] | 76.5 | |
10 | 4 | 31.9 | [31.1-32.9] | 76.5 | |
12 | 4 | 32.3 | [31.2-33.7] | 76.5 | |
50 | 10 | 3 | 33.3 | [32.7-33.7] | 76.5 |
12 | 1 | 33.6 | 76.5 |