Spatio-temporal analysis to identify determinants of Oncomelania hupensis infection with Schistosoma japonicum in Jiangsu province, China
© Yang et al.; licensee BioMed Central Ltd. 2013
Received: 22 January 2013
Accepted: 4 May 2013
Published: 6 May 2013
With the successful implementation of integrated measures for schistosomiasis japonica control, Jiangsu province has reached low-endemicity status. However, infected Oncomelania hupensis snails could still be found in certain locations along the Yangtze river until 2009, and there is concern that they might spread again, resulting in the possible re-emergence of infections among people and domestic animals alike. In order to establish a robust surveillance system that is able to detect the spread of infected snails at an early stage, sensitive and reliable methods to identify risk factors for the establishment of infected snails need to be developed.
A total of 107 villages reporting the persistent presence of infected snails were selected. Relevant data on the distribution of infected snails, and human and livestock infection status information for the years 2003 to 2008 were collected. Spatio-temporal pattern analysis including spatial autocorrelation, directional distribution and spatial error models were carried out to explore spatial correlations between infected snails and selected explanatory factors.
The area where infected snails were found, as well as their density, decreased significantly between 2003 and 2008. Changes in human and livestock prevalences were less pronounced. Three statistically significant spatial autocorrelations for infected snails were identified. (i) The Moran’s I of infected snails increased from 2004 to 2007, with the snail density increasing and the area with infected snails decreasing. (ii) The standard deviations of ellipses around infected snails were decreasing and the central points of the ellipses moved from West to East. (iii) The spatial error models indicated no significant correlation between the density of infected snails and selected risk factors.
We conclude that the contribution of local infection sources including humans and livestock to the distribution of infected snails might be relatively small and that snail control may limit infected snails to increasingly small areas ecologically most suitable for transmission. We provide a method to identify these areas and risk factors for persistent infected snail presence through spatio-temporal analysis, and a suggested framework, which could assist in designing evidence based control strategies for schistosomiasis japonica elimination.
KeywordsSchistosomiasis Infected snails Determinants Spatio-temporal analysis China
Schistosomiasis japonica is a zoonotic disease caused by an infection with Schistosoma japonicum. Oncomelania hupensis serves as the intermediate host snail of S. japonicum[1, 2]. Previous studies have shown that O. hupensis in China is mainly distributed along the Yangtze river valley and in southern China. The distribution of S. japonicum is much more restricted . Snails are infected when they are penetrated by miracidia, the larval stage of S. japonicum hatching from eggs when they reach water after being deposited with feces from the mammalian definitive hosts [4, 5]. In the People’s Republic of China, approximately 65 million individuals are currently at risk of infection with S. japonicum[6–8].
In the study presented here, we analyze the relationship between infected snail populations and local infection sources, including people and livestock in Jiangsu province based on spatio-temporal – including directional distribution – analysis, to identify the main source of infection, and explore the environmental determinants of O. hupensis infection.
The study focused on the marshland along the Yangtze river in Jiangsu province where 107 villages (Figure 1) were selected based on the following criterion: at least one infected snail had been found around the village, including in marshlands along the Yangtze river and on beaches of the rivers connected to the Yangtze river, between 2003 and 2008. The geographical co-ordinates (latitude/longitude) of each village were recorded by a GPS unit (Garmin Map76).
Between 2003 and 2008, annual surveillance covering both the human population and livestock had been carried out within the study area. In each of the study villages, all heads of livestock were examined using the standard miracidia hatching method . Among humans, 90% of the individuals aged between 6 and 60 years were screened for schistosomiasis japonica infection using a serological test (Dipstick dye immunoassay, DDIA) . Stool samples were then collected from individuals with positive test results to conduct the Kato–Katz thick smear test . A single dose of praziquantel at a dosage of 40 mg/kg body weight was offered to all seropositive individuals, and a two-day course of praziquantel at 60 mg/kg body weight was administered to those with a positive Kato-Katz thick smear test.
Snail collection was conducted by systematically sampling with a square frame of 0.11 m2 that was set every 30 meters in known snail habitats. All snails inside the frame were collected. Additionally, environmental (purposeful) sampling was employed in spring and autumn of each year to detect snails in potential snail habitats in grasslands and marshlands, e.g. where snails had been detected over the last three years, in previously flooded areas etc. Systematic sampling was then carried out if any snails were found in these potential habitats. All collected snails were counted, crushed and examined by microscopy to detect sporocysts and cercariae. Various outcome indices were considered, including the S. japonicum prevalence among humans, the rate of S. japonicum infection in snails, the density of living snails, and the density of infected snails.
Descriptive analysis was performed using the statistical software package SPSS (Version 11, SPSS Inc. Chicago, IL, USA). The analysis focused on the yearly data for S. japonicum infection among snails and the infection status of the human and livestock populations.
The spatio-temporal pattern analysis was carried out using the spatial analyst module of ArcGIS 10.0 (ESRI, Redlands, CA, USA) and GeoDA 1.0.1 (The GeoDa Center for Geospatial Analysis and Computation). The global Moran’s I was used to measure spatial autocorrelation in infected snail, human and livestock populations in each year. The spatial autocorrelation was used to evaluate whether the pattern was clustered, dispersed, or random. A Z score was considered for evaluating the significance of the Moran’s I value. The differences of spatial autocorrelation in each year were then used to explore spatio-temporal patterns.
Directional distribution, namely the Standard Deviational Ellipse (SDE), was used to measure the directional trend each year, and to provide information about dispersion of the infected snails, humans and livestock in terms of compactness and orientation. Employing the method was inspired by its wide application in diverse studies . For example, plotting ellipses for a disease outbreak over time may be used to model its spread . The distributional trend analysis can create an elliptical polygon; the attributed values for these output ellipse polygons include two standard distances (long and short axes) and the orientation of the ellipse. We used one standard deviation to represent the distribution that covers approximately 68 percent of all input variables for both the infected snails, humans and livestock [27, 28]. A series of additional measurements and data including axial ratios, and co-ordinates of each ellipse in each year were collected to compare the spatial patterns of infected snails and local infection sources.
We used a spatial autoregressive error model (a spatial regression model including a spatial autoregressive error term) implemented in GeoDA 1.0.1 to measure the relationships between the density of infected snails and the serological or stool prevalence of people and livestock. Initially, we fit the data in an ordinary least squares (OLS) regression model. As expected, the results suggested considerable non-normality and heteroscedasticity, which did not satisfy the basic hypothesis of standard linear regression, as well as high spatial correlation. Based on this result we concluded that a spatial error model was more appropriate for this dataset.
Formally, this model is y = Xβ + ϵ, with ϵ = λW + μ, where y is a vector of observations of the dependent variable, W is the spatial weights matrix, X is a matrix of observations of the explanatory variables, ϵ is a vector of spatially auto correlated error terms, μ is a vector of i.i.d. errors, and λ and β are parameters.
The study protocol was approved by the Ethics Review Committee of the Jiangsu Institute of Parasitic Diseases, Wuxi, China. Written informed consent had also been obtained from each participant or a literate relative during the screening for infections. No specific permits were required for the field studies focusing on snails as they did not involve endangered or protected species.
The characteristics of schistosomiasis japonica in the study villages of Jiangsu province, China, from 2003 to 2008
Area of infected snail habitat (ha)
Density of infected snails (/0.1m2)
Human stool examination
The yearly Moran’s I value of S. japonicum -infected snails, sero-prevalence and stool prevalence in the study villages of Jiangsu province, China, from 2003 to 2008
Human stool prevalence
Spatial error model estimations for the density of S. japonicum -infected snails in Jiangsu province, China, from 2003 to 2008
Human stool prevalence
The control of schistosomiasis japonica, similar to the control of any infectious disease, aims to interrupt the parasite lifecycle through interventions intended to eliminate the intermediate host, eliminate the parasite from the definitive host, prevent infection of the intermediate or definitive host, etc. [29, 30]. In highly endemic regions, the provision of praziquantel to the local residents is effective at reducing the infection rate [31–33]. However, this does not always interrupt transmission, as throughout history, livestock such as cattle were the main source of infection for schistosomiasis in many areas in China [34, 35]. The transmission patterns and spatial distribution of the total and infected snails and the influences of environmental and socio-economic determinants have been considered in a series of epidemiological studies supported by spatial modeling .
In Jiangsu province, the area and density of infected snails decreased significantly until 2008. The number of livestock in endemic villages also decreased significantly, to 542 in 2008 from 1001 in 2003. The prevalence in livestock was very low, with no infected livestock found in 2008. However, the prevalence in humans was stable, with no significant difference between serological and stool positive rates over the study period. This might indicate that the contribution from livestock to human infection is not as large anymore as it had been historically.
Moran’s I (Spatial Statistics) measures spatial autocorrelation based on both locations and attribute information, and evaluates whether the pattern expressed is clustered, dispersed, or random [36, 37]. In general, a Moran’s Index value near +1.0 indicates clustering while an index value near −1.0 indicates dispersion. Figure 2 shows that the Moran’s I of the sero-prevalence was between −0.075 and 0.069, indicating that the distribution was random and the sero-prevalence stable. The Moran’s I of infected snails increased from 2004 to 2007, indicating that the distribution of infected snails become more and more clustered in some regions. Indeed, the density increased from 0.373 to 0.616 infected snails per 0.1 m2, while the area with infected snails was decreasing dramatically. This suggests that schistosomiasis transmission is ongoing in certain areas and that control measures may be forcing transmission into ever smaller refugia (areas ecologically or otherwise most suitable for transmission). The variation curve of the stool prevalence also increased from 2004 to 2007. To determine whether these cluster regions were stable, the directional distribution analysis was carried out.
From 2003 to 2008, the standard deviations of ellipses around infected snail areas were decreasing and the central points of the ellipses moved from West to East, indicating that the habitats of infected snails had become smaller, and that clusters existed in special regions. The serial ellipse polygons in Figure 3A-F indicate that these spatial distributions were significantly different from each other, but the spatial correlation between infected snails and other study factors was not significant. The ellipse polygons overlap in some regions in each year, and studied factors appear to contribute to the distribution of infected snails in some regions. After integrating the spatial error model, we found that the relationship between the study factors and the spatial distribution of infected snails was not strong, confirming data from field studies. For example, no infected livestock or wild mice were detected in the infected snail habitats .
Limitations of the current study must also be recognized. First, the considered explanatory factors for infected snails were limited to the serological and parasitological status of the local human and livestock population, while the spatial distribution of infected snails may also depend on other factors, which consequently should be taken into account to improve model accuracy. Second, we explored the risk factors using retrospective data. Third, the sensitivity and specificity of serological and stool tests are not perfect [39, 40].
In conclusion, the contribution of the local infection sources including humans and livestock to the distribution of infected snails may not be significant, and external factors need further study, e.g. temporal migration from other endemic areas. It also appears that snail control may be restricting infected snails into smaller yet ecologically more suitable areas for transmission. The present study describes a way to identify risk factors through retrospective study and spatio-temporal analysis, and such a framework could assist in designing evidence-based control strategies in the process of schistosomiasis elimination.
The authors would like to acknowledge the staff at the local Center for Disease Control and Prevention Centers for their kind collaboration and making the field data available. This work received financial support from the National Natural Science Foundation of China (No. 81101275; 81101280), Natural Science Foundation of Jiangsu Province (BS2010153), Project of Public Health Department of Jiangsu Province (No. RC2011094), National S & T Major Program (No. 2012ZX10004-220), and was partially funded through a capacity building initiative for Ecohealth Research on Emerging Infectious Disease in Southeast Asia supported by the International Development Research Centre (IDRC).
- Zhou XN, Bergquist R, Leonardo L, Yang GJ, Yang K, Sudomo M, Olveda R: Schistosomiasis japonica control and research needs. Adv Parasitol. 2010, 72: 145-178.View ArticlePubMedGoogle Scholar
- Yang K, Zhou XN, Wu XH, Steinmann P, Wang XH, Yang GJ, Utzinger J, Li HJ: Landscape pattern analysis and Bayesian modeling for predicting Oncomelania hupensis distribution in Eryuan county, People’s Republic of China. Am J Trop Med Hyg. 2009, 81: 416-423.PubMedGoogle Scholar
- Lei ZL, Zheng H, Zhang LJ, Zhu R, Gao JG, Li SZ, Wang LY, Chen Z, Zhou XN: Schistosomiasis status in People’s Republic of China in 2010 (in Chinese). Chin J Schisto Control. 2011, 23: 599-604.Google Scholar
- Utzinger J, Zhou XN, Chen MG, Bergquist R: Conquering schistosomiasis in China: the long march. Acta Trop. 2005, 96: 69-96.View ArticlePubMedGoogle Scholar
- Wang LD, Utzinger J, Zhou XN: Schistosomiasis control: experiences and lessons from China. Lancet. 2008, 372: 1793-1795. 10.1016/S0140-6736(08)61358-6.View ArticlePubMedGoogle Scholar
- Zhou XN, Wang LY, Chen MG, Wu XH, Jiang QW, Chen XY, Zheng J, Utzinger J: The public health significance and control of schistosomiasis in China - then and now. Acta Trop. 2005, 96: 97-105. 10.1016/j.actatropica.2005.07.005.View ArticlePubMedGoogle Scholar
- Wang LD, Chen HG, Guo JG, Zeng XJ, Hong XL, Xiong JJ, Wu XH, Wang XH, Wang LY, Xia G: A strategy to control transmission of Schistosoma japonicum in China. N Engl J Med. 2009, 360: 121-128. 10.1056/NEJMoa0800135.View ArticlePubMedGoogle Scholar
- Zhou XN, Guo JG, Wu XH, Jiang QW, Zheng J, Dang H, Wang XH, Xu J, Zhu HQ, Wu GL: Epidemiology of schistosomiasis in the People’s Republic of China, 2004. Emerg Infect Dis. 2007, 13: 1470-1476. 10.3201/eid1310.061423.PubMed CentralView ArticlePubMedGoogle Scholar
- Hong QB, Huang YX, Zhou XN, Sun LP, Xia DH, Xu GK, Tian QA, Tian QA, Zhu JM: Status of schistosomiasis transmission and longitudinal observation on impact of control program in marshland of Jiangsu province. Chin J Schisto Control. 2001, 11: 34-38.Google Scholar
- Huang YX, Sun LP, Hong QB, Gao Y, Zhang LH, Gao Y, Chen H, Guo JH, Liang YS, Zhu YC: Longitudinal observation on fluctuation trend of distribution and spread of Oncomelania snails after floodwater in marshland of lower beaches of Yangtze Rive (in Chinese). Chin J Schisto Control. 2004, 16: 253-256.Google Scholar
- Li SZ, Luz A, Wang XH, Xu LL, Wang Q, Qian YJ, Wu XH, Guo JG, Xia G, Wang LY, Zhou XN: Schistosomiasis in China: acute infections during 2005–2008. Chin Med J (Engl). 2009, 122: 1009-1014.Google Scholar
- Yuan H, Jiagang G, Bergquist R, Tanner M, Xianyi C, Huanzeng W: The 1992–1999 World Bank Schistosomiasis Research Initiative in China: outcome and perspectives. Parasitol Int. 2000, 49: 195-207. 10.1016/S1383-5769(00)00045-3.View ArticlePubMedGoogle Scholar
- Huang YX, Manderson L: The social and economic context and determinants of schistosomiasis japonica. Acta Trop. 2005, 96: 223-231. 10.1016/j.actatropica.2005.07.015.View ArticlePubMedGoogle Scholar
- Sun LP, Liang YS, Huang YX, Hong QB, Yang G, Zhang LH, Gao Y, Xie CY: Evaluation on effect of infection control of schistosomiasis in Jiangsu province (in Chinese). Chin J Schisto Control. 2008, 20: 13-17.Google Scholar
- Yang GJ, Vounatsou P, Zhou XN, Tanner M, Utzinger J: A Bayesian-based approach for spatio-temporal modeling of county level prevalence of Schistosoma japonicum infection in Jiangsu province, China. Int J Parasitol. 2005, 35: 155-162. 10.1016/j.ijpara.2004.11.002.View ArticlePubMedGoogle Scholar
- Sun LP, Wang W, Liang YS, Tian ZX, Hong QB, Yang K, Yang GJ, Dai JR, Gao Y: Effect of an integrated control strategy for schistosomiasis japonica in the lower reaches of the Yangtze River, China: an evaluation from 2005 to 2008. Parasit Vectors. 2011, 4: 243-10.1186/1756-3305-4-243.PubMed CentralView ArticlePubMedGoogle Scholar
- Zhou YB, Yang MX, Yihuo WL, Liu GM, Wang HY, Wei JG, Jiang QW: Effect of habitat fragmentation on the schistosome-transmitting snail Oncomelania hupensis in a mountainous area of China. Trans R Soc Trop Med Hyg. 2011, 105: 189-196. 10.1016/j.trstmh.2010.12.006.View ArticlePubMedGoogle Scholar
- Li SZ, Wang YX, Yang K, Liu Q, Wang Q, Zhang Y, Wu XH, Guo JG, Bergquist R, Zhou XN: Landscape genetics: the correlation of spatial and genetic distances of Oncomelania hupensis, the intermediate host snail of Schistosoma japonicum in mainland China. Geospat Health. 2009, 3: 221-231.View ArticlePubMedGoogle Scholar
- Yang K, Wang XH, Yang GJ, Wu XH, Qi YL, Li HJ, Zhou XN: An integrated approach to identify distribution of Oncomelania hupensis, the intermediate host of Schistosoma japonicum, in a mountainous region in China. Int J Parasitol. 2008, 38: 1007-1016. 10.1016/j.ijpara.2007.12.007.View ArticlePubMedGoogle Scholar
- Malone JB, Yang GJ, Leonardo L, Zhou XN: Implementing a geospatial health data infrastructure for control of Asian schistosomiasis in the People’s Republic of China and the Philippines. Adv Parasitol. 2010, 73: 71-100.View ArticlePubMedGoogle Scholar
- Zhou XN, Lv S, Yang GJ, Kristensen TK, Bergquist NR, Utzinger J, Malone JB: Spatial epidemiology in zoonotic parasitic diseases: insights gained at the 1st International Symposium on Geospatial Health in Lijiang, China, 2007. Parasit Vectors. 2009, 2: 10-10.1186/1756-3305-2-10.PubMed CentralView ArticlePubMedGoogle Scholar
- Scott LM, Janikas MV: Spatial statistics in ArcGIS. Handbook App Spatial Analysis. 2010, 27-41.View ArticleGoogle Scholar
- Zhang YY, Luo JP, Liu YM, Wang QZ, Chen JH, Xu MX, Xu JM, Wu J, Tu XM, Wu GL: Evaluation of Kato-Katz examination method in three areas with low-level endemicity of schistosomiasis japonica in China: a Bayesian modeling approach. Acta Trop. 2009, 112: 16-22. 10.1016/j.actatropica.2009.05.020.View ArticlePubMedGoogle Scholar
- Jia XM, Sriplung H, Chongsuvivatwong V, Geater A: Sensitivity of pooled serum testing for screening antibody of schistosomiasis japonica by IHA in a mountainous area of Yunnan, China. Parasitology. 2009, 136: 267-272. 10.1017/S0031182008005489.View ArticlePubMedGoogle Scholar
- Gong J: Clarifying the standard deviational ellipse. Geographical Analysis. 2002, 34: 155-167.View ArticleGoogle Scholar
- The ESRI Guide to GIS Analysis. Edited by: Mitchell A. 2005, Redlands: ESRI PressGoogle Scholar
- Naparus M, Kuntner M: A GIS model predicting potential distributions of a lineage: a test case on hermit spiders (Nephilidae: Nephilengys). PLoS One. 2012, 7: e30047-10.1371/journal.pone.0030047.PubMed CentralView ArticlePubMedGoogle Scholar
- Svensson K, Back E, Eliasson H, Berglund L, Granberg M, Karlsson L, Larsson P, Forsman M, Johansson A: Landscape epidemiology of tularemia outbreaks in Sweden. Emerg Infect Dis. 2009, 15: 1937-1947. 10.3201/eid1512.090487.PubMed CentralView ArticlePubMedGoogle Scholar
- Seto EY, Remais JV, Carlton EJ, Wang S, Liang S, Brindley PJ, Qiu D, Spear RC, Wang LD, Wang TP: Toward sustainable and comprehensive control of schistosomiasis in China: lessons from Sichuan. PLoS Negl Trop Dis. 2011, 5: e1372-10.1371/journal.pntd.0001372.PubMed CentralView ArticlePubMedGoogle Scholar
- King CH: Toward the elimination of schistosomiasis. N Engl J Med. 2009, 360: 106-109. 10.1056/NEJMp0808041.View ArticlePubMedGoogle Scholar
- Chen MG: Use of praziquantel for clinical treatment and morbidity control of schistosomiasis japonica in China: a review of 30 years’ experience. Acta Trop. 2005, 96: 168-176. 10.1016/j.actatropica.2005.07.011.View ArticlePubMedGoogle Scholar
- Guo J, Li Y, Gray D, Ning A, Hu G, Chen H, Davis GM, Sleigh AC, Feng Z, McManus DP, Williams GM: A drug-based intervention study on the importance of buffaloes for human Schistosoma japonicum infection around Poyang lake, People’s Republic of China. Am J Trop Med Hyg. 2006, 74: 335-341.PubMedGoogle Scholar
- Zhou XN, Guo JG, Wu XH, Jiang QW, Zheng J, Dang H, Wang XH, Xu J, Zhu HQ, Wu GL: Epidemiology of schistosomiasis in the People’s Republic of China, 2004. Emerg Infect Dis. 2007, 10: 1470-1477.View ArticleGoogle Scholar
- Wang TP, Vang Johansen M, Zhang SQ, Wang FF, Wu WD, Zhang GH, Pan XP, Ju Y, Ornbjerg N: Transmission of Schistosoma japonicum by humans and domestic animals in the Yangtze River valley, Anhui province, China. Acta Trop. 2005, 96: 198-204. 10.1016/j.actatropica.2005.07.017.View ArticlePubMedGoogle Scholar
- Yuan HC: Epidemiological features and control strategies of schistosomiasis japonica in China. Chin Med J (Engl). 1993, 106: 563-568.Google Scholar
- Tsai PJ, Lin ML, Chu CM, Perng CH: Spatial autocorrelation analysis of health care hotspots in Taiwan in 2006. BMC Public Health. 2009, 9: 464-10.1186/1471-2458-9-464.PubMed CentralView ArticlePubMedGoogle Scholar
- Hui FM, Xu B, Chen ZW, Cheng X, Liang L, Huang HB, Fang LQ, Yang H, Zhou HN, Yang HL: Spatio-temporal distribution of malaria in Yunnan province, China. Am J Trop Med Hyg. 2009, 81: 503-509.PubMedGoogle Scholar
- Yu Q, Dar JR, Wu RF, Bao ZP, Gao JG, Liang YS, Liang YS: Prevalence study on source of infection of schistosomiasis in marshland with infected snails in lower reaches of Yangtze River. Chia J Schisto Control. 2010, 22: 351-254.Google Scholar
- Wang XH, Wu XH, Zhou XN: Bayesian estimation of community prevalences of Schistosoma japonicum infection in China. Int J Parasitol. 2006, 36: 895-902. 10.1016/j.ijpara.2006.04.003.View ArticlePubMedGoogle Scholar
- Wang XH, Zhou XN, Vounatsou P, Chen Z, Utzinger J, Yang K, Steinmann P, Wu XH: Bayesian spatio-temporal modeling of schistosoma japonicum prevalence data in the absence of a diagnostic ‘Gold’ standard. PLoS Negl Trop Dis. 2008, 2: e250-10.1371/journal.pntd.0000250.PubMed CentralView ArticlePubMedGoogle Scholar
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