Figure 1From: Multilocus microsatellite signature and identification of specific molecular markers for Leishmania aethiopicaIdentification of L. aethiopica from clinical isolates. A) PCR amplification of Leishmania genomic DNA from different species using previously developed primers MATRAE2 and Ae2.1 subjected to electrophoresis on a 1% agarose gel. Lanes 1 and 2 are PCR products from 2 different L. aethiopica isolates used in the study. Lanes 3, 4, 5, 6, 7 and 8 are PCR products amplified from genomic DNA of L. donovani, L. major, L. mexicana, L. tropica, L. infantum and mouse, respectively. L is 1kb DNA ladder. B) Basic Local Alignment search of sequences derived from PCR amplification of the ITS1 region of L aethiopica, L major and L. tropica, using L5.8S/LISTR primers. Genomic DNA from L. aethiopica MHOM/ET/1972/L102 was used as a reference strain.Back to article page