An improved autocidal gravid ovitrap for the control and surveillance of Aedes aegypti
© Mackay et al.; licensee BioMed Central Ltd. 2013
Received: 12 April 2013
Accepted: 30 July 2013
Published: 6 August 2013
Limited success has been achieved using traditional vector control methods to prevent the transmission of dengue viruses. Integrated control programs incorporating alternative tools, such as gravid ovitraps (lethal ovitraps and sticky ovitraps) may provide greater potential for monitoring and reducing vector populations and dengue virus transmission. We had developed an autocidal gravid ovitrap (AGO) as a simple, low-cost device for surveillance and control of Ae. aegypti without the use of pesticides that does not require servicing for an extended period of time. The purpose of our study was to improve the efficacy and efficiency of this device.
Competitive assays were performed in the laboratory and an outdoor cage to evaluate whether modifications to the structure and appearance of our original trap design (AGO-A), and the addition of an olfactory bait (hay infusion), improve trap function. The performance of a modified trap design (AGO-B) was then assessed and compared with conventional ovitraps in a series of field tests in San Juan City, Puerto Rico. Generalized linear mixed models were used to analyze adult Ae. aegypti capture data from the laboratory, outdoor cage and field experiments.
Increasing the size of the trap entrance, altering the color of trap components, and increasing the volume/surface area of the aqueous bait significantly improved the performance of the AGO in the outdoor cage. In a subsequent field comparison, captures of Ae. aegypti females were 3.7 fold greater in the improved trap (AGO-B), compared with the original design (AGO-A). An infusion bait produced “in situ” significantly improved capture rates of the improved trap under both semi-natural and field conditions. Semi-weekly collections of Ae. aegypti females in the AGO-B were significantly correlated with cumulative rainfall 8 to 28 days prior to sampling, whereas egg collections in paired conventional ovitraps were not. When vector abundance was low, the AGO-B provided greater sensitivity and precision as a surveillance device, compared with paired conventional ovitraps.
The AGO-B can be used to efficiently attract and capture gravid Ae. aegypti females for more than 8 weeks without the need for trap maintenance.
KeywordsAedes aegypti Oviposition Sticky ovitraps Vector surveillance Puerto Rico
Access to practical tools for the surveillance and suppression of adult Aedes aegypti L. is critical to developing an effective and sustainable dengue vector control program [1, 2]. Surveillance of Ae. aegypti is primarily limited to sampling its immature stages. Little effort is made to directly reduce the survival of adult vectors at a community scale, except for truck-mounted, ultra-low volume applications of insecticides in public and peridomestic spaces, which may have limited efficacy against Ae. aegypti[3–6]. There is a need to identify alternative tools and strategies for cost-effective control and surveillance of adult vectors.
The ovitrap has been used for many decades as a sensitive, inexpensive, passive surveillance tool for detecting the presence of container-inhabiting mosquitoes, and for providing a relative measure of temporal changes in adult abundance. The addition of a larvicide or autocidal mechanism allows long-term use of the ovitrap with minimal risk of the device becoming a productive source of adult mosquitoes (ie., autocidal ovitrap, eg. [7, 8]). Ovitraps can also be modified to collect gravid females by incorporating an adhesive capture surface (sticky ovitraps; eg. ). Adult mosquitoes collected in sticky ovitraps provide a direct measure of adult abundance, may be identified by morphology in the field, and can be processed for the detection of pathogens or host blood meal identification [10–12]. Sampling with sticky ovitraps is a more sensitive method for detecting the presence of Ae. aegypti in comparison with sampling of immatures [13, 14]. Though they tend to capture fewer mosquitoes than BG-sentinel traps  and provide a less representative sample of the adult population than backpack aspirators , sticky ovitraps can be a more practical and affordable alternative for routine vector surveillance. Indices based on the number of gravid females collected in sticky traps have been used to describe the spatial-temporal distribution of Ae. aegypti and dengue virus (DENV) transmission risk [10, 14, 17–19].
In addition to their value for vector surveillance, various ovitrap devices have been evaluated as tools for suppressing Ae. aegypti populations [7, 8, 20, 21]. Lethal ovitraps, which contain an oviposition substrate treated with a residual insecticide (eg., ), and sticky ovitraps eliminate gravid female mosquitoes as they attempt to oviposit inside the trap (both referred to collectively hereafter as “gravid ovitraps”; GOs), thereby reducing the daily survival rate of the fraction of the adult vector population most likely to be infective and potentially lowering vectorial capacity. A potential disadvantage of using an insecticide rather than an adhesive in a GO is that efficacy could be compromised when the device is used against a vector population with a low susceptibility to the insecticide class.
A potential limitation of using ovitrap devices for vector control is their ability to compete with existing container habitats in the environment [20, 23, 24]. It has been recommended that trap deployment should be preceded by a community-wide source reduction effort to mitigate this effect [20, 25, 26]. Furthermore, variability in the abundance and quality of alternative aquatic habitats in time and space likely diminishes the precision of ovitraps to estimate adult abundance . Negative effects of competing oviposition sites on trap efficacy might be lessened by increasing the relative attractiveness of the ovitrap device. Typically ovitraps are constructed from relatively small containers (capacity 0.5-2 l). Previous studies have reported a positive association between container size, and the frequency of positivity and the number of eggs deposited by Ae. aegypti[28–30]. A larger ovitrap may provide a more conspicuous visual target to gravid females searching for suitable oviposition sites, and a greater release rate of water vapor and other volatile attractants, potentially extending the range of attractive olfactory stimuli released by a trap. Ovitrap devices with a larger capacity may also be less susceptible to failure due to desiccation of the aqueous attractant.
Aqueous infusions of decaying botanical material, prepared either prior to trap deployment or in situ, and synthetic blends of compounds identified from infusions of decaying plants, have been shown to significantly increase the number of Ae. aegypti eggs collected in ovitraps under field conditions [31–33], and therefore are often used as baits in GOs [20, 34, 35]. However, the relative contributions of olfactory attraction and contact-mediated oviposition stimulants to enhanced oviposition in infusion baited traps are unclear. Field studies comparing numbers of adult Ae. aegypti captured in sticky ovitraps baited with water and plant-based infusions have not shown these baits to enhance attraction to the trap [16, 36].
The objective in this study was to improve upon an autocidal gravid ovitrap device (AGO-A) that we had developed as a low-cost and practical device for routine surveillance and control of Ae. aegypti mosquitoes. In a previous area-wide, integrated vector control intervention (source reduction and larviciding) in two communities in southern Puerto Rico, inclusion of the AGO-A as part of the intervention resulted in a significantly greater reduction (≈43%) in the relative abundance of parous and gravid Ae. aegypti, compared with the community where only source reduction and larviciding was performed (CDC, unpublished data). Following these encouraging results, we evaluated several modifications intended to improve the efficacy and efficiency of the trap. We also evaluated the contribution of an olfactory bait (hay infusion generated in situ) to trap performance over an extended use period (> 6 weeks). In this paper we describe how our improved, autocidal gravid ovitrap design (AGO-B) was developed and tested under laboratory and field conditions in Puerto Rico.
Gravid females used in behavioral assays under laboratory and semi-natural conditions were from the F2 to F5 generations of colonies established from eggs collected from Patillas, Puerto Rico (18° 0′ 27″ N, 66° 0′ 35″ W). Individual cohorts were reared at 26 ± 1.0 °C, 65–80 percent relative humidity, and a photoperiod of 12:12 (L:D), in pans containing 150 larvae and 1 liter of dechlorinated water, and fed increasing amounts of finely ground rabbit chow during development (0.7-1.6 mg per larva per day). A minimum of 3 days after adult emergence (range 3-7d), females were artificially fed on bovine or porcine blood daily for five consecutive days, then maintained for an additional 3 days prior to use in behavioral assays (range 10-14d post-emergence). A cotton wick provided 10 percent sucrose solution from adult emergence to 1 day prior to the 1st day of blood feeding, and from the 4th day of blood feeding to the day prior to use.
Competitive assays under semi-natural conditions
Trap designs and infusion baits were compared in competitive assays under semi-natural conditions in two field cages. Most of these experiments were performed in Cage A, a 10 m diameter, 3.4 m high geodesic dome tent (Shelter Systems, Santa Cruz, California, USA), with two 0.2 × 1.5 m and four 1.5 × 1.5 m screen windows providing ventilation. Traps were arranged at fixed locations in a ring 1.6 m from the wall of the field cage, with a minimum distance of 2.6 m (6 or 8 traps) or 4.8 m (3 or 4 traps) between traps. A single experiment (attraction to anaerobically fermented hay infusion in the AGO-B; see below) was conducted in a smaller, rectangular field enclosure (Cage B; 7.0 × 2.7 × 3.7 m) with screen walls on two adjacent sides. In Cage B, a trap was assigned to each of two locations approximately 2 m apart in the center of the enclosure.
In each trial, 120 (Cage B) or 150 (Cage A) gravid Ae. aegypti females were released from the center of the arena. Experiments were conducted from 2.5 hrs prior to sunset, and traps were recovered 2.5 hrs after sunrise, corresponding to the diel rhythm of oviposition activity of gravid Ae. aegypti females . A sweep net and BG-sentinel trap (operated for 6 hours) were used to collect females remaining in the cage after each trial.
Data from the competitive assays in the screened room and field cage were analyzed by generalized linear mixed models (GLMMs) with repeated measures (SPSS ver. 12.0, SPSS, Inc., Chicago, Illinois, USA), with number of females captured as the response variable, treatment as a fixed effect, and trap location (subject) and trial (repeated measure) as random effects. Results are reported as marginal means with 95 percent confidence intervals. Bonferroni adjustments were made for assays comparing more than two treatments.
Autocidal gravid ovitraps
Development of the AGO-B
The transition from the initial trap design (AGO-A) to the final model (AGO-B) was directed by the results of a series of competitive assays conducted under semi-natural conditions (Cage A) assessing the influence of changes to the structure and appearance of the trap on the number of gravid females captured. In each experiment, traps were baited with a 1:1 dilution of an anaerobically-fermented (AF) hay infusion prepared in a closed container similar to the method of , except that the concentration of hay was reduced to 3.8 g per l of water. For each trial, 61 g of Cynodon nlemfuensis Vanderyst (Bogdan) was added to 16 l of dechlorinated tap water in a sealed (19 l capacity) plastic container. Infusions were aged for 7 days in a shaded location outdoors under ambient conditions, then sieved with a 1.2 mm mesh prior to use, and diluted with dechlorinated tap water (1:1). The diluted infusion was added to the infusion reservoir to ≈ 80 percent of maximum capacity (AGO-A = 2 l; AGO-B = 8 l). The autocidal oviposition substrate (PAM) was prepared by hydrating Outdoor Variety Plant Gel crystals (DNB Designs, Inc., Colorado Springs, Colorado, USA) with undiluted AF infusion (1 g / 100 ml infusion) for a minimum of 3 hours prior to each experiment. The PAM acts as an attractive surface for oviposition while preventing immature development (CDC, unpublished data).
In the first test, the effects of trap entrance diameter (ED) and the color of the CS on trap performance were examined. The numbers of gravid Ae. aegypti females captured in the AGO-A (8.8 cm ED + white CS) were compared with captures in three modified AGO-A designs; (i) 8.8 cm ED + black CS, (ii) 12 cm ED + white CS, and (iii) 12 cm ED + black CS. A black, plastic truncated cone, of identical height (11 cm) and similar shape as the 8.8 cm ED cup, was used to create the entrance of trap designs (ii) and (iii). In each of 6 trials, two traps representing each treatment were randomly assigned to eight fixed locations in the field cage.
The second experiment assessed the combined effects of altering the shape of the trap entrance, the position of the CS, and the size of the infusion reservoir (volume and surface area of infusion) on trap performance by comparing capture rates between the AGO-B (but with a white pail lid and without a ¾” exclusion screen) and the modified AGO-A design (iii) from the previous experiment (12 cm ED, black CS). For each of three trials, four traps of each type were randomly distributed among 8 fixed locations in the field cage.
In the third experiment, the influence of a color contrast between the trap entrance and the pail lid on the performance of the AGO-B was examined. Two AGO-Bs with a white lid, and two AGO-Bs with a black lid, were randomly placed at 4 fixed locations in each of eight trials. The ¾” exclusion screen was not used in this test.
The fourth experiment was performed to test whether capture efficiency is reduced by the use of an exclusion screen to prevent larger organisms or debris (leaves, etc.) from entering the trap. In each of three trials, AGO-Bs were placed at eight fixed locations, and a ¾” exclusion screen was used to cover the trap entrance of four randomly selected traps.
Attractiveness of hay infusion bait produced in situ
Two initial experiments were performed under semi-natural conditions to verify that the use of “standard” AF infusion bait significantly enhances attraction to both AGO models. In the first test, AGO-As were baited with either tap water or a 1:1 dilution of AF infusion, prepared as described previously. In each of five trials, four AGO-As representing each treatment group were randomly assigned to one of eight fixed locations in Cage A. This experiment was repeated in Cage B using the AGO-B. Eight replicate trials were performed in the second experiment.
After verifying that the “standard” AF infusion bait enhances the capture of gravid Ae. aegypti females in both trap designs, three sets of experiments were performed in Cage A to assess the attractiveness of hay infusion fermented in situ by adding a packet of dry hay to the infusion reservoir filled to ≈ 80 percent of maximum capacity with dechlorinated tap water (3.8 g hay / l of water). Hay packets were prepared by folding the dry hay into a compact form and wrapping it with a plastic cable tie.
In the first experiment, the relative attractiveness of infusion produced in situ using a hay packet, in both the AGO-A and the AGO-B, was compared with our standard bait (1:1 dilution of AF infusion). The AF and in situ infusions were prepared as described previously. After aging for 7 days, each infusion was removed from its respective receptacle, sieved and 1.5 l was transferred to a 3.8 l plastic container, which was placed inside an AGO-B (no additional water or infusion was added to the infusion reservoir). For each trial (n = 6), an AGO-B representing each infusion type was randomly assigned to one of three fixed locations in the field cage.
The second test of an in situ infusion evaluated the influence of hay concentration on attraction to the AGO-B. Traps were baited with 8 l of tap water and either 3.8 g or 8.3 g of hay per l (higher concentration from ), then aged under natural conditions in a shaded location. Assays were performed at 7 and 28 days, with three replicate trials performed at each time point. In each trial, four AGO-Bs with the low hay concentration, and four AGO-Bs with the high hay concentration were randomly assigned to one of the eight fixed locations in the field cage. The numbers of gravid females released per trial was reduced to 120 in the first time point (7 days).
It was observed in both the laboratory and field tests that gravid females will often expel their eggs when they are trapped on the CS of the AGO-A and AGO-B (“death stress oviposition”; ). We were concerned that odors released by con-specific larvae in the infusion could increase attraction to the trap, resulting in a potential sampling bias (ie. relative attractiveness of trap positively correlated with number of females previously captured). To investigate this, AGO-Bs baited with an in situ infusion (30g hay packet) were aged under field conditions for 7 days in a shaded location. Five days prior to testing, 0, 50 or 500 first instar Ae. aegypti larvae were added to the infusion reservoir of each trap. For each trial (n = 9), a single AGO-B representing each larval density was randomly assigned to one of three fixed locations.
Field assessments of the AGO-B
Three consecutive field experiments evaluating the performance of the AGO-B were conducted in 2011 in the San Juan metropolitan area, Puerto Rico. The study site (“El Comandante”) was a residential neighborhood approximately 1.5 km2 in size (described in ). The 30 residential properties selected for trap placement were uniformly distributed, with an average minimum distance of 145 m between selected properties. At each selected property, 2 AGOs (1 per treatment) were placed at fixed positions that provided some protection from direct sunlight (ie. next to the home or vegetation), and were at least 5 m apart, on opposite sides of the property when possible. Every Thursday and Monday, adult mosquitoes were removed from the CSs and enumerated by species and sex, and the positions of the two treatments were rotated within each property. To facilitate consistent 3 day sampling intervals, the trap entrance was sealed with window screens from Thursday to Friday to prevent entry of mosquitoes. A pair of conventional ovitraps (“enhanced pairs” of ) were placed at a home adjacent to each selected property to provide an additional measure of Ae. aegypti activity in the study area. Ovitraps were replaced at the start of each 3 day sampling period. Meteorological data was collected at the Luis-Muñoz International Airport, approximately 4 km from the study site.
In the first field experiment, numbers of adult mosquitoes captured in the original trap model (AGO-A) and the improved device (AGO-B) were compared from 15 February to 18 April. Traps, constructed as described previously, were baited with a 30 g hay packet (in situ infusion) and distributed among the 30 selected properties. One property was later excluded from the experiment after home renovations prevented access to the traps.
The second field experiment (18 April to 12 May) was initiated immediately following the first experiment to assess whether restoration of attraction to the AGO-B after extended use requires a full replacement of the in situ infusion bait, or if it would be sufficient to replace the hay packet and the water lost via evaporation, but retain the fluid remaining in the infusion reservoir (partial replacement). Fresh traps (designated AGO-B) were prepared as described above to represent a “full bait replacement”. The “partial bait replacement” condition was generated by replacing the CSs, PAM and hay packets in the AGO-B devices used in the previous experiment (now designated AGO-Bp). Tap water was added to each AGO-Bp to bring the volume up to maximum capacity (9.3 l).
In the third field experiment (6 June to 21 July), we examined the relative contribution of the in situ infusion to trap performance during extended use (> 6 weeks) by comparing adult captures in traps baited with a hay packet (AGO-B) and traps without a hay packet (only water; AGO-Bw). Samples were collected from AGOs at all 30 selected properties in this experiment.
A GLMM with a Poisson distribution and log link function was constructed for each field experiment to evaluate the influence of AGO trap design (fixed effect) on the number of Ae. aegypti females collected per 3 day period (SPSS, Inc.). Trap location within each home was included as a random effect. Covariates evaluated in each model included cumulative rainfall and average daily mean air temperature 1 to 7, 1 to 21, 1 to 28, 8 to 21 and 8 to 28 days preceding the sampling period. These time intervals were chosen based on previously observed temporal relationships between the abundance of Ae. aegypti females (measured by BG-sentinel sampling), and rainfall and temperature and at the same study site . The −2 log likelihood ratios of nested models were compared to identify the best fit model for each experiment.
Optimal sample size calculations for the AGO-B and paired ovitraps
Data collected from the field experiments were used to calculate the optimal number of AGO-B devices required for estimating the relative abundance of Ae. aegypti adult females. Taylor’s Power Law equations (log10s2 = log10 (a) + b log10 (m); ) were used to regress the log10 transformed variance (s2) and mean values (m) for each sampling date, in each field experiment, for both devices (GraphPad Prism version 5.04 for Windows, GraphPad Software, San Diego, California, USA). Regression coefficients from individual experiments were compared , and data from experiments with coefficients that were not significantly different were pooled. Coefficients of a Taylor’s Power Law regression model of the pooled data set were used to calculate the minimum sample size (N) required to estimate female Ae. aegypti relative abundance at a range of precision levels (0.1, 0.2, 0.3) using the formula: N = (Zα/2 / D)2 ⋅ am(b-2), where Zα/2 is the upper α/2 part of the standard normal distribution, D is the specified precision level (fixed proportion of the mean) and m is the expected number of specimens collected per trap per day . Identical analyses were performed with data collected in the paired ovitraps to estimate the optimal number of conventional ovitrap pairs required for sampling eggs. To directly compare estimated minimum sample sizes between the two sampling devices, a major axis regression was used to model the relationship between the mean adult collections in the AGO-B and mean egg collections in the paired ovitraps (SPSS, Inc.).
Long-term efficacy of the AGO-B
At the conclusion of field experiment 1 (62 days), we conducted a census of the condition of AGO-Bs, and the volume of infusion remaining in each trap was measured. It was observed that the CS of the AGO-B becomes coated with many small flies, dust and other debris after extended use. Used CSs from twelve randomly selected AGO-Bs were brought back to the laboratory to test whether their ability to capture gravid females had diminished after extended use.
To test whether the efficacy of the adhesive was reduced, a 2-way competitive assay was conducted under laboratory conditions (≈26°C, 70% RH, 12:12 L:D) in a 3.8 × 2.7 × 2.9 m room. In each of 4 trials, 3 AGO-Bs with a fresh CS, and 3 AGO-Bs with a 62 day old CS (from field experiment 1) were used. Traps were randomly assigned to 6 fixed locations forming a 2 × 3 m grid. One hundred and twenty gravid Ae. aegypti females were released from the center of the room 3 hours prior to the start of the scotophase, and the numbers captured in each trap were determined 20 hours later. Eggs deposited on the PAM were also collected and counted. The numbers of adults and eggs collected in each trap were compared as described previously for the field cage tests. A square root transformation of the egg data was used to approximate a normal distribution.
Development of the AGO-B
When the modified AGO-A design (12 cm ED and black CS) was compared with the AGO-B in competitive assays in the field cage, trap design was found to significantly influence capture efficiency (F = 21.0, P < 0.001). Mean (95% CI) numbers of females captured per trap in competitive assays in the field cage were 13.1 (10.9, 15.3) and 20.0 (17.8, 22.2) in the modified AGO-A and the AGO-B designs, respectively.
Lid color had a significant influence on trap captures in the AGO-B (F = 11.3, P < 0.01). The mean (95% CI) number of females captured in traps with a black lid was 37.4 (34.0, 40.9), compared with 29.5 (26.0, 32.9) in traps with a white lid.
In the field cage, the addition of a ¾” exclusion screen to the trap entrance had a significant positive effect on the capture of female Ae. aegypti in the AGO-B (F = 6.1, P < 0.05). Mean (95% CI) numbers of females captured in traps with and without an exclusion screen were 20.2 (17.7, 22.8) and 16.2 (13.7, 18.8), respectively.
Attractiveness of hay infusion bait produced in situ
Influence of infusion on attraction to AGOs
Avg. females per trap (95% CI)
7.5 (4.1, 10.9)
18.8 (15.4, 22.2)
33.0 (16.2, 49.8)
62.6 (45.9, 79.4)
Influence of ageing-conditions, hay concentration and conspecific odors on attraction to infusion-baited AGO-Bs
Infusion age (days)
Avg. females per trap (95% CI)
1 - Infusion fermentation conditions
46.1 (40.7, 51.6)
In situ (AGO-A)
37.4 (31.9, 42.8)
In situ (AGO-B)
36.3 (30.9, 41.7)
2 - Hay concentrationa
In situ (1×)
12.1 (9.6, 14.6)
In situ (2.2×)
10.1 (8.1, 13.1)
In situ (1×)
19.4 (17.0, 21.7)
In situ (2.2×)
16.2 (13.8, 18.6)
3 - Addition of conspecific larvae
34.9 (29.1, 40.6)
In situ + 50 larvae
39.7 (33.9, 45.4)
In situ + 500 larvae
41.1 (35.4, 46.9)
Field assessments of the AGO-B
Precision and sensitivity of ovitraps and AGOs and relationships between capture rate and rainfall
Field experiment (na)
Avg. specimens / trap / day (95% CI)
Avg. proportion samples positive (95% CI)
Spearman correlation with rain 8to28c
Generalized linear mixed models comparing influences of AGO design and meterological conditions on capture rate
Exp 1. AGO-B vs AGO-A
Exp 2. AGO-B vs AGO-Bp
Exp 3. AGO-B vs AGO-Bw
Optimal sample size calculations
Optimal sample size calculations
D = 0.1
D = 0.2
D = 0.3
D = 0.1
D = 0.2
D = 0.3
Long-term efficacy of the AGO-B
Capture efficiency of aged capture surfaces (CSs)
CS age (days)
Avg. per trap (95% CI)
16.4 (12.6, 20.2)
20.9 (17.1, 24.7)
Eggs collected per captured female
15.0 (8.2, 23.8)
13.6 (7.2, 22.1)
Our objective in this study was to improve our original autocidal gravid ovitrap device so that it was a more efficacious and practical tool for vector surveillance and control. Several trap designs intermediate to the AGO-A and AGO-B were compared in order to assess sequential changes to the appearance, the trap entrance, and volume/surface area of the attractant. We also assessed the contribution of a hay infusion bait to trap performance.
Several trap devices use contrasting black and white surfaces to improve capture of Ae. aegypti[15, 44, 45]. However we found that the AGO-B was most effective when constructed entirely of black materials - increasing the visual contrast of the trap entrance by the use of a white pail lid did not improve capture efficiency. In competitive assays in the field cage, enlarging the size of the infusion reservoir and trap entrance significantly enhanced trap performance. Likely these modifications promoted orientation and entry of females approaching the trap entrance in part via a greater release of water vapor and other attractive compounds from the infusion. In laboratory tests, females were often observed making several rapid, short flights that transversed the air space directly above the entrance prior to entry. It has been suggested that a similar behaviour observed in Toxorhynchites spp. during oviposition (“looping flight”) allows females to detect a humidity gradient that indicates the presence of a potential oviposition site . Unexpectedly, the addition of a ¾” mesh over the entrance of the AGO-B did not appear to impede entry of mosquitoes, rather it improved trap performance.
Previous assessments of infusion baits have not shown that they enhance capture of adult Ae. aegypti in sticky ovitraps in a natural setting [16, 36]. Zang and Lei  observed that a Bermuda-grass infusion bait significantly increased attraction of Ae. albopictus adults to sticky traps in the laboratory, but not in a subsequent field test. The authors concluded that in a natural setting, the influence of infusion odor on preoviposition behavior may be overshadowed by visual stimuli. In our field cage tests, a 1:1 dilution of a week-old, anaerobically-fermented infusion made from hay (C. nlemfuensis) increased attraction by ≈ 2.5 fold in the AGO-A, and ≈ 2 fold in the AGO-B, relative to traps containing only tap water. Similarly addition of a hay packet to the AGO-B increased capture of female Ae. aegypti by an average of 1.6 fold in the third field experiment. In the field cage experiment, infusions aged in an open container (ie., in situ) were less attractive than infusions produced under anaerobic conditions, as previously reported [48, 49].
Attraction of gravid Ae. aegypti females to plant-based infusions is influenced by the type and concentration of organic material used, and the length of time and conditions under which the infusion is aged [48, 49]. Ponnusamy et al. demonstrated that these factors influence the diversity and abundance of microorganisms associated with attraction. In ovitrap devices, the addition of plant material as a substrate for microbial growth (ie., in situ infusion) can extend the duration of olfactory attraction between replacement of the infusion bait . However, as the organic material is consumed, the attractiveness of the infusion odor may diminish due to biochemical changes in the infusion resulting from succession in the microbial community. Variability in relative attractiveness over time could limit the precision of surveillance data collected using infusion-baited ovitraps. In our third field experiment, the relative difference between captures in water baited and infusion baited AGO-Bs was consistent over time, suggesting that the attractiveness of the in situ infusion to gravid Ae. aegypti females was not diminished after more than 6 weeks of use. This would indicate that changes in infusion odor are unlikely to introduce a strong bias in surveillance data collected with the AGO-B during long-term use.
Several studies have reported that waters containing (or previously containing) conspecific larvae can enhance oviposition by Ae. aegypti females, in comparison with distilled or tap water [51–53]. Semiochemicals released by conspecific larvae or bacteria associated with the larvae may also act as attractants to gravid females [54, 55]. In the current study, adding up to 500 larvae to the infusion attractant did not significantly enhance attraction to the AGO-B. Benzon and Apperson  observed peak oviposition by Ae. aegypti in water that had previously held 1 conspecific larva per ml; a much greater density than the one assayed in this study (0.0625 larvae per ml). However, the purpose of our experiment was to determine if colonization of the AGO-B by the progeny of captured females could influence the attractiveness of the trap. In practice, the density of larvae in an AGO-B is expected to be far less than 1 larva per ml. Though the density was not quantified, none of the traps that were found positive for Ae. aegypti immatures after extended field use (62 days) appeared to hold more than 100 larvae or pupae (<0.02 larvae / ml).
There are conflicting descriptions of the response of Ae. aegypti adult populations to rainfall. While some studies have observed that adult abundance and oviposition activity are positively associated with precipitation [56, 57], others have failed to detect a relationship [13, 58] or have found other meteorological factors to be more predictive . Some of this discrepancy may be attributed to local or regional differences in the relative importance of aquatic habitats receiving water directly from human activities versus container habitats filled by rainfall . In metro San Juan, PR, both human-managed and rain-filled containers are important for Ae. aegypti development . In the current study, there were significant associations between changes in the capture rate in the AGO-B and cumulative rainfall 8 to 28 days preceding sampling, consistent with a previous study at the same location where numbers of Ae. aegypti collected in BG-sentinel traps were positively correlated with an 8 to 21 day lag in cumulative rainfall . Interestingly, no relationship was detected between rainfall and egg collections in the paired ovitraps. The strength of the relationship between rainfall and adult captures in the AGO-B was not consistent among the three field tests; the strongest association was evident in the first two field tests when rainfall intensity and frequency were lower. Similar seasonal variability in the strength of the response to rainfall has been reported in other populations of Ae. aegypti[60, 61].
At lower vector densities (field experiment 1), the AGO-B provided a more sensitive measure of adult activity, relative to paired ovitraps, with fewer AGO-Bs required to monitor Ae. aegypti populations with an equivalent level of precision. These results suggest that the AGO-B might be a more practical surveillance tool for programs designed to detect the introduction and spread of Ae. aegypti to new areas (eg. ). However at higher vector densities (field experiments 2 and 3), the AGO-B and paired ovitraps had equivalent precision and sensitivity. An inverse relationship was reported by Faccinelli et al., who observed a similar or higher level of precision and sensitivity using a sticky ovitrap, compared with conventional ovitraps, for sampling Ae albopictus at moderate to high densities, but not at low densities. Other field assessments with sticky ovitrap devices have reported them to be equally or less sensitive for detecting the presence of Ae. aegypti, relative to conventional ovitrap sampling [34, 57].
Although the absence of a common, independent measure of the underlying density of adult vectors makes it difficult to make direct comparisons with previous field studies evaluating similar passive trapping devices, our field data suggest that the AGO-B has a relatively high proficiency to attract and capture Ae. aegypti females. Most other field studies with sticky ovitraps have observed average capture rates of less than 0.2 Ae. aegypti females per trap per day [13, 16, 18, 19, 34, 35, 64–66], although collection rates of greater than 3 females per trap per day have been reported . These sticky ovitraps are much smaller than the AGO-B, with a maximum capacity of a few hundred ml to 2 l of water or infusion bait. This apparent advantage in the capture rate of the AGO-B over similar devices could be due to the larger infusion reservoir, as evidenced by the results of the field cage studies, where the AGO-B captured several fold more adult females than a modified AGO-A with a similar appearance and trap entrance diameter, but smaller infusion reservoir.
We achieved a substantial inprovement in capture rate by modifying our original autocidal gravid ovitrap. Results of the three field tests validate that capture efficiency and relative attractiveness of the modified trap (AGO-B) can be maintained by periodic (≈ every 6–8 weeks) replacement of the capture surface, hay substrate and water lost to evaporation. Our original device (AGO-A) had previously been found to significantly reduce the abundance of potentially infected (gravid and parous) females of Ae aegypti when used concurrently with source reduction and larvicide applications in an area wide vector control program (CDC, unpublished data). We expect that the improved device (AGO-B) will have an even greater impact on vector populations than observed with the AGO-A. The low cost (materials ≈ $6.50 per trap) and its ability to be used for extended periods without maintenance could permit long-term, large scale deployment of these devices. Addionally, the high sensitivity for detecting the presence of Ae. aegypti at low density also demonstrates the potential value of the AGO-B for monitoring re-infestation or recovery following an area wide application of integrated vector control efforts. Future research efforts will include a robust, community scale assessment of the AGO-B as a vector control and surveillance tool.
Autocidal gravid ovitrap
Generalized linear mixed model
Polyacrylamide copolymer hydrogel.
We would like to express our gratitude for the expert assistance with the field and laboratory research provided by Veronica Aceveda, Angel Berrios, Belkis Caban, Gilberto Felix, Jesus Flores, Jose Gonzales, Orlando Gonzalez, Juan F. Medina and Luis Riviera. We would also like to thank the residents of El Comandante for their patience and hospitality.
- Morrison AC, Zielinski-Gutierrez E, Scott TW, Rosenberg R: Defining challenges and proposing solutions for control of the virus vector Aedes aegypti. PLoS Med. 2008, 5: e68-10.1371/journal.pmed.0050068.PubMed CentralView ArticlePubMedGoogle Scholar
- Knox TB, Scott TW: Vector Control for Prevention of Dengue: Current Status and Future Strategies. Advances in Human Vector Control. Edited by: Clark J, Bloomquist J, Kawada H. 2009, Washington: American Chemical Society Books, 39-57.View ArticleGoogle Scholar
- Fox I: Evaluation of ultra-low volume aerial and ground applications of malathion against natural populations of Aedes aegypti in Puerto Rico. Mosq News. 1980, 40: 280-283.Google Scholar
- US Centers for Disease Control and Prevention: Efficacy of adulticides against Aedes aegypti in Puerto Rico. 1987, San Juan: Dengue Surveillance Summary, 44-Google Scholar
- Focks DA, Kloter KO, Carmichael GT: The impact of sequential ultra-low volume ground aerosol applications of malathion on the population dynamics of Aedes aegypti (L.). Am J Trop Med Hyg. 1987, 36: 639-647.PubMedGoogle Scholar
- Clark GG, Reiter P, Gubler DJ: Aedes aegypti control trials using aerial ULV applications. Arbovirus Research in Australia. Proceedings of the Fifth Symposium. Edited by: Uren MF, Blok J, Manderson LH. 1990, Brisbane: Australia Commonwealth Scientific and Industrial Research Organization, 192-193.Google Scholar
- Chan KL: The eradication of Aedes aegypti at the Singapore Paya Lebar International Airport. Vector Control In Southeast Asia. Edited by: Yow Cheong C, Kai Lok C, Beng Chuan H. 1973, Singapore: Sen Wah Press, 85-88.Google Scholar
- Regis L, Monteiro AM, de Melo-Santos MAV, Silveira JC, Furtado AF, Acioli RV, Santos GM, Nakazawa M, Carvalho MS, Ribeiro PJ, de Souza WV: Developing new approaches for detecting and preventing Aedes aegypti population outbreaks: Basis for surveillance, alert and control system. Mem Inst Oswaldo Cruz. 2008, 103: 50-59. 10.1590/S0074-02762008000100008.View ArticlePubMedGoogle Scholar
- Vartak PH, Tungikar VB, Sharma RN: Laboratory evaluation of an ovipositional trap for mosquito adults Aedes aegypti (L.) (Diptera: Culicidae). J Com Dis. 1995, 27: 32-35.Google Scholar
- Ritchie SA, Long S, Smith G, Pyke A, Knox TB: Entomological investigations in a focus of dengue transmission in Cairns, Queensland, Australia, by using the sticky ovitraps. J Med Entomol. 2004, 41: 1-4. 10.1603/0022-2585-41.1.1.View ArticlePubMedGoogle Scholar
- Bangs MJ, Pudiantari R, Gionar YR: Persistence of dengue virus RNA in dried Aedes aegypti (Diptera: Culicidae) exposed to natural tropical conditions. J Med Entomol. 2007, 44: 163-167. 10.1603/0022-2585(2007)44[163:PODVRI]2.0.CO;2.View ArticlePubMedGoogle Scholar
- Valerio L, Marini F, Bongiorno G, Facchinelli L, Pombi M, Caputo B, Maroli M, Della Torre A: Host-feeding patterns of Aedes albopictus (Diptera: Culicidae) in urban and rural contexts within Rome Province, Italy. Vector-Borne Zoonotic Dis. 2010, 10: 291-294. 10.1089/vbz.2009.0007.View ArticlePubMedGoogle Scholar
- Gama RA, Silva EM, Silva IM, Resende MC, Eiras AE: Evaluation of the sticky MosquiTRAP™ for detecting Aedes (Stegomyia) aegypti (L.) (Diptera: Culicidae) during the dry season in Belo Horizonte, Minas Gerais, Brazil. Neotrop Entomol. 2007, 36: 294-302. 10.1590/S1519-566X2007000200018.View ArticlePubMedGoogle Scholar
- Steffler LM, Marteis LS, Dolabella SS, Cavalcanti SCH, Santos RLC: Risk of dengue occurrence based on the capture of gravid Aedes aegypti females using MosquiTRAP. Mem Inst Oswaldo Cruz. 2011, 106: 365-367.View ArticlePubMedGoogle Scholar
- Krökel U, Rose A, Eiras AE, Geier M: New tools for surveillance of adult yellow fever mosquitoes: Comparison of trap catches with human landing rates in an urban environment. J Am Mosq Control Assoc. 2006, 22: 229-238. 10.2987/8756-971X(2006)22[229:NTFSOA]2.0.CO;2.View ArticleGoogle Scholar
- Maciel-de-Freitas R, Peres RC, Alves F, Brandolini MB: Mosquito traps designed to capture Aedes aegypti (Diptera: Culicidae) females: Preliminary comparison of Adultrap, MosquiTRAP and backpack aspirator efficiency in a dengue-endemic area of Brazil. Mem Inst Oswaldo Cruz. 2008, 103: 602-605.View ArticlePubMedGoogle Scholar
- Eiras AE, Resende MC: Preliminary evaluation of the “Dengue-MI” technology for Aedes aegypti monitoring and control. Cad Saude Publica. 2009, 25 (Suppl. 1): S45-S58.View ArticlePubMedGoogle Scholar
- Wu HH, Wang CY, Teng HJ, Lin C, Lu LC, Jian SW, Chang NT, Wen TH, Wu JW, Liu DP, Lin LJ, Norris DE, Wu HS: A dengue vector surveillance by human population-stratified ovitrap survey for Aedes (Diptera: Culicidae) adult and egg collections in high dengue-risk areas of Taiwan. J Med Entomol. 2013, 50: 261-269. 10.1603/ME11263.View ArticlePubMedGoogle Scholar
- Lee C, Vythilinggam I, Chong CS, Razak MAA, Tan CH, Pok KY, Ng LC: Gravitraps for management of dengue clusters in Singapore. Am J Trop Med Hyg. 2013, 10.4269/ajtmh.12-0329. Published online March 11, 2013Google Scholar
- Perich MJ, Kardec A, Braga IA, Portal IF, Burge R, Zeichner BC, Brogdan WA, Wirtz RA: Field evaluation of a lethal ovitrap against dengue vectors in Brazil. Med Vet Entomol. 2003, 17: 205-210. 10.1046/j.1365-2915.2003.00427.x.View ArticlePubMedGoogle Scholar
- Rapley LP, Johnson PH, Williams CR, Silcock RM, Larkman M, Long SA, Russell RC, Ritchie SA: A lethal ovitrap-based mass trapping scheme for dengue control in Australia: II. Impact on populations of the mosquito Aedes aegypti. Med Vet Entomol. 2009, 23: 303-316. 10.1111/j.1365-2915.2009.00834.x.View ArticlePubMedGoogle Scholar
- Zeichner BC, Perich MJ: Laboratory testing of a lethal ovitrap for Aedes aegypti. Med Vet Entomol. 1999, 13: 234-238. 10.1046/j.1365-2915.1999.00192.x.View ArticlePubMedGoogle Scholar
- Sithiprasasna R, Mahapibul P, Noigamol C, Perich MJ, Zeichner BC, Burge B, Norris SL, Jones JW, Schleich SS, Coleman RE: Field evaluation of a lethal ovitrap for the control of Aedes aegypti (Diptera: Culicidae) in Thailand. J Med Entomol. 2003, 40: 455-462. 10.1603/0022-2585-40.4.455.View ArticlePubMedGoogle Scholar
- Williams CR, Ritchie SA, Long SA, Dennison N, Russell RC: Impact of a bifenthrin-treated lethal ovitrap on Aedes aegypti oviposition and mortality in North Queensland, Australia. J Med Entomol. 2007, 44: 256-262. 10.1603/0022-2585(2007)44[256:IOABLO]2.0.CO;2.View ArticlePubMedGoogle Scholar
- Knipling EF: A theoretical appraisal of the value of ovitraps for the suppression of container-breeding mosquitoes. W H O /VBC/70. 1970, 186: 1-14.Google Scholar
- Chan KL, Kiat NS, Koh TK: An autocidal ovitrap for the control and possible eradication of Aedes aegypti. Southeast Asian J Trop Med Pub Hlth. 1977, 8: 56-61.Google Scholar
- Focks DA: Special Programme for Research and Training in Tropical Diseases (TDR). A Review of Entomological Sampling Methods and Indicators of Dengue Vectors. 2003, Geneva: World Health Organization, TDR/IDE/Den/03.1Google Scholar
- Bond HA, Fay RW: Factors influencing Aedes aegypti occurrence in containers. Mosq News. 1969, 29: 113-116.Google Scholar
- Harrington LC, Ponlawat A, Edman JD, Scott TW, Vermeylen F: Influence of container size, location, and time of day on oviposition patterns of the dengue vector, Aedes aegypti, in Thailand. Vector-Borne Zoonotic Dis. 2008, 8: 415-422. 10.1089/vbz.2007.0203.PubMed CentralView ArticlePubMedGoogle Scholar
- Wong J, Stoddard ST, Astete H, Morrison AC, Scott TW: Oviposition site selection by the dengue vector Aedes aegypti and its implications for dengue control. PLoS Negl Trop Dis. 2011, 5: e1015-10.1371/journal.pntd.0001015.PubMed CentralView ArticlePubMedGoogle Scholar
- Reiter P, Amador MA, Colon N: Enhancement of the CDC ovitrap with hay infusions for daily monitoring of Aedes aegypti populations. J Am Mosq Control Assoc. 1991, 7: 52-55.PubMedGoogle Scholar
- Ritchie SA: Effect of some animal feeds and oviposition substrates on Aedes oviposition in ovitraps in Cairns, Australia. J Am Mosq Control Assoc. 2001, 17: 206-208.PubMedGoogle Scholar
- Barbosa RMR, Furtado A, Regis L, Leal WS: Evaluation of an oviposition-stimulating kairomone for the yellow fever mosquito, Aedes aegypti, in Recife. Brazil J Vector Ecol. 2010, 35: 204-207. 10.1111/j.1948-7134.2010.00078.x.View ArticlePubMedGoogle Scholar
- Ritchie SA, Long S, Hart A, Webb CE, Russell RC: An adulticidal sticky ovitrap for sampling container-breeding mosquitoes. J Am Mosq Control Assoc. 2003, 19: 235-242.PubMedGoogle Scholar
- Favaro EA, Dibo MR, Mondini A, Ferreira AC, Barbosa AAC, Eiras AE, Barata EAMF, Chiaravalloti-Neto F: Physiological state of Aedes (Stegomyia) aegypti mosquitoes captured with MosquiTRAPs® in Mirrasol, San Paulo, Brazil. J Vector Ecol. 2006, 31: 285-291. 10.3376/1081-1710(2006)31[285:PSOASA]2.0.CO;2.View ArticlePubMedGoogle Scholar
- Russell RC, Ritchie SA: Surveillance and behavioral investigations of Aedes aegypti and Aedes polynesiensis in Moorea, French Polynesia, using a sticky ovitrap. J Am Mosq Control Assoc. 2004, 20: 370-375.PubMedGoogle Scholar
- Chadee DD, Corbet PS: The gonotrophic status and diel pattern of entry to outdoor oviposition sites of female Aedes aegypti (L.) (Diptera: Culicidae). Ann Trop Med Parasitol. 1993, 87: 263-268.PubMedGoogle Scholar
- Chadee DD, Ritchie SA: Oviposition behaviour and parity rates of Aedes aegypti collected in sticky traps in Trinidad. West Indies Acta Tropica. 2010, 116: 212-216. 10.1016/j.actatropica.2010.08.008.View ArticlePubMedGoogle Scholar
- Barrera R: Spatial stability of adult Aedes aegypti populations. Am J Trop Med Hyg. 2011, 85: 1087-1092. 10.4269/ajtmh.2011.11-0381.PubMed CentralView ArticlePubMedGoogle Scholar
- Barrera R, Amador M, Mackay AJ: Population dynamics of Aedes aegypti and dengue as influenced by weather and human behavior in San Juan. Puerto Rico PLoS Negl Trop Dis. 2011, 5: e1378-10.1371/journal.pntd.0001378.View ArticlePubMedGoogle Scholar
- Taylor LR: Aggregation, variance and the mean. Nature. 1961, 189: 732-735. 10.1038/189732a0.View ArticleGoogle Scholar
- Zar JH: Biostatistical Analysis. 1984, Englewood Cliffs: Prentice-Hall, 2Google Scholar
- Wilson LT, Room PM: Clumping patterns of fruit and arthropods in cotton, with implications for binomial sampling. Environ Entomol. 1983, 12: 50-54.View ArticleGoogle Scholar
- Fay RW, Prince WH: A visual trap for Aedes aegypti. Mosq News. 1970, 30: 20-23.Google Scholar
- Freier JE, Francy DB: A duplex cone trap for the collection of adult Aedes albopictus. J Am Mosq Control Assoc. 1991, 7: 73-79.PubMedGoogle Scholar
- Benzon GL, Apperson CS, Clay W: Factors affecting oviposition site preference by Toxorhynchites splendens in the laboratory. J Am Mosq Control Assoc. 1988, 4: 20-22.PubMedGoogle Scholar
- Zang LY, Lei CL: Evaluation of sticky ovitraps for the surveillance of Aedes (Stegomyia) albopictus (Skuse) and the screening of oviposition attractants from organic infusions. Ann Trop Med Parasitol. 2008, 102: 399-407. 10.1179/136485908X300878.View ArticleGoogle Scholar
- Sant’Ana AL, Roque RA, Eiras AE: Characteristics of grass infusions as oviposition attractants to Aedes (Stegomyia) (Diptera: Culicidae). J Med Entomol. 2006, 43: 214-220. 10.1603/0022-2585(2006)043[0214:COGIAO]2.0.CO;2.Google Scholar
- Ponnusamy L, Xu N, Boroczky K, Wesson DM, Abu Ayyash L, Schal C, Apperson CS: Oviposition responses of the mosquitoes Aedes aegypti and Aedes albopictus to experimental plant infusions in laboratory bioassays. J Chem Ecol. 2010, 36: 709-719. 10.1007/s10886-010-9806-2.PubMed CentralView ArticlePubMedGoogle Scholar
- Ponnusamy L, Wesson DM, Arellano C, Schal C, Apperson CS: Species composition of bacterial communities influences attraction of mosquitoes to experimental plant infusions. Microb Ecol. 2010, 59: 158-173. 10.1007/s00248-009-9565-1.PubMed CentralView ArticlePubMedGoogle Scholar
- Soman RS, Reuben R: Studies on the preference shown by ovipositing females of Aedes aegypti for water containing immature stages of the same species. J Med Entomol. 1970, 7: 485-489.View ArticlePubMedGoogle Scholar
- Sucharit S, Tumrasvin W: Ovipositional attractancy of waters containing larvae of Aedes aegypti and Aedes albopictus. Jap J Sanit Zool. 1981, 32: 261-264.Google Scholar
- Allan SA, Kline DL: Larval rearing water and preexisting eggs influence oviposition by Aedes aegypti and Ae. albopictus (Diptera: Culicidae). J Med Entomol. 1998, 35: 943-947.View ArticlePubMedGoogle Scholar
- Seenivasagan T, Sharma KR, Sekhar K, Ganesan K, Prakash S, Vijayaraghavan R: Electroantennogram, flight orientation, and oviposition responses of Aedes aegypti to the oviposition pheromone n-heneicosane. Parasitol Res. 2009, 104: 827-833. 10.1007/s00436-008-1263-2.View ArticlePubMedGoogle Scholar
- Benzon GL, Apperson CS: Reexamination of chemically mediated oviposition behavior in Aedes aegypti (L.) (Diptera: Culicidae). J Med Entomol. 1988, 25: 158-164.View ArticlePubMedGoogle Scholar
- Dibo MR, Chierotti AP, Ferrari MS, Mendonca AL, Neto FC: Study of the relationship between Aedes (Stegomyia) aegypti egg and adult densities, dengue fever and climate in Mirassol, state of San Paulo, Brazil. Mem Inst Oswaldo Cruz. 2008, 103: 554-560.View ArticlePubMedGoogle Scholar
- Honorio NA, Codeco CT, Alves FC, Magalhaes MAMF, Lourenco-De-Oliveira R: Temporal distribution of Aedes aegypti in different districts of Rio De Janeiro, Brazil, measured by two types of traps. J Med Entomol. 2009, 46: 1001-1014. 10.1603/033.046.0505.View ArticlePubMedGoogle Scholar
- Scott TW, Morrison AC, Lorenz LH, Clark GG, Strickland D, Kittayapong P, Zhou H, Edman JD: Longitudinal studies of Aedes aegypti (Diptera: Culicidae) in Thailand and Puerto Rico: Population dynamics. J Med Entomol. 2000, 37: 77-88. 10.1603/0022-2585-37.1.77.View ArticlePubMedGoogle Scholar
- Azil AH, Long SA, Ritchie SA, Williams CR: The development of predictive tools for pre-emptive dengue vector control: A study of Aedes aegypti abundance and meteorological variables in North Queensland, Australia. Trop Med Int Hlth. 2010, 15: 1190-1197. 10.1111/j.1365-3156.2010.02592.x.View ArticleGoogle Scholar
- Ho BC, Chan KL, Chan YC: Aedes aegypti (L.) and Aedes albopictus (Skuse) in Singapore City. 3. Population fluctuations. Bull Wld Hlth Org. 1971, 44: 635-641.Google Scholar
- Triplis M: Seasonal variation in adult populations of Aedes aegypti in the Dar Es Salaam area, Tanzania. W H O /VBC/71. 1971, 291: 1-29.Google Scholar
- Schaffner F, Bellini R, Petrić D, Scholte E, Zeller H, Rakotoarivony LM: Development of guidelines for the surveillance of invasive mosquitoes in Europe. Parasit Vectors. 2013, 6: 209-10.1186/1756-3305-6-209.PubMed CentralView ArticlePubMedGoogle Scholar
- Facchinelli L, Valerio L, Pombi M, Reiter P, Costantini C, Dellatorre A: Development of a novel sticky trap for container breeding mosquitoes and evaluation of its sampling properties to monitor urban populations of Aedes albopictus. Med Vet Entomol. 2007, 21: 183-195. 10.1111/j.1365-2915.2007.00680.x.View ArticlePubMedGoogle Scholar
- Williams CR, Long SA, Russell RC, Ritchie SA: Optimizing ovitrap use for Aedes aegypti in Cairns, Queensland, Australia: Effects of some abiotic factors on field efficacy. J Am Mosq Control Assoc. 2006, 22: 635-640. 10.2987/8756-971X(2006)22[635:OOUFAA]2.0.CO;2.View ArticlePubMedGoogle Scholar
- Facchinelli L, Koenraadt CJM, Fanello C, Kijchalao U, Valerio L, Jones JW, Scott TW, Della Torre A: Evaluation of a sticky trap for collecting Aedes (Stegomyia) adults in a dengue-endemic area in Thailand. Am J Trop Med Hyg. 2008, 78: 904-909.PubMedGoogle Scholar
- de Santos EMM, de Melos-Santos MAV, de Oliveira CMF, Correia JC, de Albuquerque CMR: Evaluation of a sticky trap (AedesTraP), made from disposable plastic bottles, as a monitoring tool for Aedes aegypti populations. Parasit Vectors. 2012, 5: 195-10.1186/1756-3305-5-195.PubMed CentralView ArticlePubMedGoogle Scholar
- Chadee DD, Ritchie SA: Efficacy of sticky and standard ovitraps for Aedes aegypti in Trinidad, West Indies. J Vector Ecol. 2010, 35: 395-400. 10.1111/j.1948-7134.2010.00098.x.View ArticlePubMedGoogle Scholar
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