Grouping facilitates avoidance of parasites by fish
© Mikheev et al.; licensee BioMed Central Ltd. 2013
Received: 21 March 2013
Accepted: 8 October 2013
Published: 17 October 2013
Parasite distribution is often highly heterogeneous, and intensity of infection depends, among other things, on how well hosts can avoid areas with a high concentration of parasites. We studied the role of fish behaviour in avoiding microhabitats with a high infection risk using Oncorhynchus mykiss and cercariae of Diplostomum pseudospathaceum as a model. Spatial distribution of parasites in experimental tanks was highly heterogeneous. We hypothesized that fish in groups are better at recognizing a parasitized area and avoiding it than solitary fish.
Number of fish, either solitary or in groups of 5, was recorded in different compartments of a shuttle tank where fish could make a choice between areas with different risk of being infected. Intensity of infection was assessed and compared with the number of fish recorded in the compartment with parasites and level of fish motility.
Both solitary fish and fish in groups avoided parasitized areas, but fish in groups avoided it more strongly and thus acquired significantly fewer parasites than solitary fish. Prevalence of infection among grouped and solitary fish was 66 and 92 %, respectively, with the mean abundance two times higher in the solitary fish. Between-individual variation in the number of parasites per fish was higher in the “groups” treatment (across all individuals) than in the “solitary” treatment. Avoidance behaviour was more efficient when fish were allowed to explore the experimental arena prior to parasite exposure. High motility of fish was shown to increase the acquisition of D. pseudospathaceum.
Fish in groups better avoided parasitized habitat, and acquired significantly fewer parasites than solitary fish. We suggest that fish in groups benefit from information about parasites gained from other members of a group. Grouping behaviour may be an efficient mechanism of parasite avoidance, together with individual behaviour and immune responses of fishes. Avoidance of habitats with a high parasite risk can be an important factor contributing to the evolution and maintenance of grouping behaviour in fish.
Parasites are known to affect the physiology, behaviour and life history of their hosts, over both evolutionary and ecological scales [1–4], and hosts exhibit various counter-adaptations to deal with parasites . Host behaviour plays an important role in defence against parasites [4, 5]. A broad array of host behaviours, such as escape responses, changes in habitat choice and social behaviours, are involved in parasite avoidance [6–8]. Increased parasitism has been considered one of the costs of sociality . However, sociality may also include benefits in terms of defence against parasites, such as lower exposure due to dilution effects when living in groups [10, 11].
Fish are able to avoid parasitized habitats where large and easily recognizable parasites such as Argulus canadensis occur . Many infective free-swimming parasite stages, like trematode cercariae, are small. Their first intermediate hosts, snails or clams, shed “clouds” of tiny cercariae, which are often very unevenly distributed. This makes detection and avoidance of trematode cercariae difficult. Individual fish may only become aware of the parasite’s presence when irritated by penetrating cercariae [13, 14]. Juvenile rainbow trout, Oncorhynchus mykiss, experimentally exposed to cercariae of the trematode Diplostomum spathaceum, fled from the site of high parasite concentration shortly after penetration of the first few cercariae . However, the persistence of such avoidance behaviour is unclear. One or a few D. spathaceum metacercariae established in fish eyes will not cause serious damage [15, 16], but as more cercariae are accumulated in a fish, the more deleterious are the effects [15, 16]. Fish should thus avoid acquisition of numerous parasites.
Grouping behaviour of fish is well known to be crucial in anti-predator defence, i.e. in avoidance of sites with high predation risk [11, 17], but its role in anti-parasite defence is poorly understood . In the presence of Diplostomum spp. cercariae, freshwater fish formed tighter shoals than in the absence of these parasites . Shoaling in sticklebacks is an effective mechanism, acting through the dilution effect, to avoid the ectoparasite Argulus canadensis. A meta-analysis revealed that the intensity of infection with mobile parasites consistently declined with group size . However, to our knowledge, the role of fish grouping in avoiding areas with high risk of infection has not been studied. Finding such an effect would indicate that fish may be able to obtain information about spatial risk of parasite exposure by observing other members of the shoal. Indeed, learned defence (i.e. social learning) has been shown to take place in fish shoals; for example, information about predation risk is socially transmitted from the observer(s) to the other members of the group [11, 21].
We have used an experimental arena (a shuttle tank where fish could make a choice between areas with different levels of risk of being infected) and an experimental fish-parasite model (juvenile Oncorhynchus mykiss and Diplostomum pseudospathaceum) to study if microhabitat selection could help fish to avoid parasitized areas when the parasite distribution is highly clumped. Our hypothesis is that fish in groups could better recognize and avoid parasitized areas than solitary fish.
Fish and parasites
Fish were obtained from a commercial fish farm. Fish were reared in indoor tanks supplied with ground water. The fish were free of D. pseudospathaceum infection. Mean fish fork length ± s.e. was 91.5 ± 8.62 mm (Experiment 1) and 93.8 ± 8.26 mm (Experiment 2). The mean body lengths were not significantly different between 'groups’ and 'solitary fish’ treatments. Prior to the experiments, about 300 fish were kept in a flow-through tank of 2.5 m3 on 8:16 L : D cycle at 16°C; they were fed with commercial pelleted food (1.5 mm size, Nutra Parr LB, Norway).
Cercariae of D. pseudospathaceum were obtained from 8 naturally infected Lymnaea stagnalis snails collected from Lake Konnevesi. D. pseudospathaceum is the only diplostomid species found in this snail in Lake Konnevesi [22, 23]. We pooled all cercariae produced within 6 hours and estimated their density from ten 1-ml subsamples of the suspension. Infectivity of D. pseudospathaceum cercariae does not decrease even 10 hours after shedding at 20°C .
Experimental set-up and procedure
The experiments were conducted at the Konnevesi Research Station, University of Jyväskylä in July – August 2006. Two experiments were conducted to study avoidance of a parasitized area by solitary and grouped fish. In the first experiment, fish were not allowed to explore the experimental arena prior to the beginning of testing. In the second experiment, fish were allowed to explore the experimental arena for 120-min prior to testing. The number of parasites acquired by fish was estimated. Young-of-the-year rainbow trout Oncorhynchus mykiss were used for the experiments.
Four flow-through dark brown 3-compartment tanks (total length × width × height 170 × 30 × 40 cm, volume 180 l) were used. The compartments contained no stones or cover and the bottom was flat and plain. The two end compartments (70 × 30 × 40 cm each) were connected to the central one (30 × 30 × 40 cm) by rectangular holes of 5 × 3 cm near the bottom. The holes could be closed and opened with raising doors to control the passage of fish from one compartment to another. The central compartment was used as a start chamber. In trials with parasites, cercariae were added to a randomly chosen end compartment and filtered lake water to the opposite end compartment. In control trials (without parasites), filtered lake water without parasites was added to both end compartments. At the beginning of each test, the water flow was turned off. Oxygen saturation was 90–97% and it did not decrease by more than 1 – 3% by the end of experiment. Water temperature was kept at 15-16°C; illumination was 300 lux. The water was removed and the tanks were thoroughly cleaned and rinsed between the trials.
In our preliminary tests, strong vertical and weak horizontal dispersion of D. pseudospathaceum cercariae was observed see also . Cercariae were placed in a 100-ml bottle on the bottom of the aquarium (40 × 30 × 20 cm). After 30 min, a majority of them left the bottle and concentrated in the upper and, to a lesser extent, near-bottom layers in the vicinity (about 5 cm) of the bottle. To check if a difference in cercariae concentration between “parasitized” and “opposite” compartments of the experimental tanks still existed by the end of a 3 h exposure, five 100-ml samples were randomly taken from each compartment. From 2 to 15 cercariae were found in samples from the parasitized compartment, while only once 2 cercariae were found in the whole set of samples from the opposite compartment. This suggests that the substantial difference in cercariae concentration between the end compartments was maintained until the end of the 3 h experiment.
To study if fish avoided a parasitized compartment, the number of fish in the two end compartments and central compartment was recorded every 15 min over 3 hrs (Experiment 1) and over 30 min (Experiment 2). Each recording consisted of 3 consecutive counts with a 1 – min interval between them. The sum of the three counts for each 15 min was used for further analysis. For simplicity, this sum will then be called “number of fish recorded” (NFR, ind). The more time a fish spent in a certain compartment, the higher the probability it would be recorded there, and the higher the obtained NFR would be for this compartment.
In this experiment, we studied 1) if fish could avoid a parasitized area and 2) if they did it better when in groups than as solitary fish. Fish were allowed to settle in the central compartment for 15 min, then the doors were gently raised. A suspension of D. pseudospathaceum cercariae in filtered lake water was slowly added through a tube (diameter 2.5 mm) into a randomly chosen end compartment. Filtered lake water without parasites was added in the same way to the opposite end compartment. Three hundred millilitres of suspension containing 12,000 cercariae were released over 20 minutes. In control tests, instead of parasite suspension, filtered lake water without parasites was added to both end compartments.
The first recording was done 15 min after the doors were opened. Observers monitored fish from behind a screen through slits. Four control tests and 4 tests where fish were exposed to parasites were run daily from 11 to 16 o’clock. In total, we carried out 32 replicates with solitary fish (16 with parasites, 16 control) and 20 with groups of 5 fish (10 with parasites, 10 control).
In this experiment, we studied how solitary fish and fish in groups avoided a parasitized compartment and assessed the number of parasites acquired by each fish. Fish were allowed to get familiar with the experimental arena over two hours before the test started. A bottle of 240 ml lake water containing 12,000 D. pseudospathaceum cercariae was placed in the parasitized end compartment. A bottle of 240 ml filtered lake water without parasites was placed in the opposite end compartment.
Each trial lasted for 30 min. We assessed NFR for the parasitized and nonparasitized compartments (24 replicates for solitary fish and 24 replicates for fish in groups of 5). We also assessed the motility of the fish in groups (24 replicates). The number of fish in motion and resting motionless on the bottom was counted at every recording. If 3 or more fish out of 5 were in motion at the recording point, the whole group was scored as of “high motility”. If 3 or more fish of 5 were motionless, the whole group was scored as of “low motility”. For each group of 5 fish, 9 such scores were accumulated by the end of the 30-min observation. If 5 or more of the 9 scores were “high motility”, the group was considered as a “high motility” group.
After exposure, fish were transferred to 150 l flow-through tanks where they were kept for 2 days, which is the time needed for D. pseudospathaceum metacercariae to develop in the eye lenses to an easily recognizable size. Then the fish were killed by an overdose of MS222, measured, and inspected for the number of parasites in the eye lenses. The number of established metacercariae was counted microscopically for all the 24 fish in the tests on solitary fish and 120 fish in the tests on groups.
The NFR values for the end compartments were pooled either for the whole 180-min, or for the first and second 90-min periods separately (Experiment 1), or for the whole 30-min period (Experiment 2), and they were used as response variables in statistical analyses. For comparisons between solitary and grouped fish, the values for the groups of 5 fish were divided by 5.
Two-way ANOVA with effects of parasites (presence or absence) and fish group (solitary vs group) was used to compare the NFR values for different compartments of the experimental tanks. The data were checked for normality and homogeneity of variances and met the assumptions of ANOVA. LSD test was used for post-hoc comparisons.
Mann–Whitney U test was used to compare the number of parasites acquired by solitary fish and fish in groups, and by high and low motility fish groups in Experiment 2. Spearman correlation analysis was used to assess the relationship between the number of acquired metacercariae and the NFR for the parasitized compartment. All analyses were conducted with STATISTICA 6.0 software.
We used 0+ Oncorhynchus mykiss. The level of experimental D. pseudospathaceum infection was maintained at a much lower level than maximum values reported for naturally occurring infections (up to 200–500 ind fish-1) [26, 27]. The mortality of infected fish in these experiments was less than 1% and did not exceed that of control fish. No visible damage was observed in any fish. We minimized the required number of animals that were killed and dissected. Experimental fish were killed at the end of the tests with an overdose of MS 222, and dissected. In total, 210 experimentally infected fish were killed. The experiments were conducted with permission of the Lab-Animal Care and Use Committee of the University of Jyväskylä (licence number 30/30.5.2005).
There was no significant difference in avoidance of the parasitized compartment between solitary fish and fish in groups when the NFR values were pooled for the whole 180-min period (Two-way ANOVA, F1,1 = 0.97; P = 0.329; LSD post-hoc test for the NFR in tests on solitary fish vs fish in groups: P = 0.097) (Figure 1A, B). Fish need 1 to 2 hours to explore novel experimental surroundings [28, 29]. During that time, exploration is an activity of highest priority [30, 31] and could override parasite avoidance behaviour. That is why we analyzed the first and second 90-min periods separately. No difference in the NFR in the tests with solitary fish vs groups of 5 fish was found for the first 90 minutes (Two-way ANOVA, F1,1 = 0.008; P = 0.929. LSD post-hoc test: P = 0.628). During the last 90 minutes, the NFR (parasitized compartment) for tests on fish in groups was significantly less than for tests on solitary fish (Two-way ANOVA, F1,1 = 2.83; P = 0.099. LSD post-hoc test: P = 0.027), indicating that fish in groups better avoided the parasitized compartment in the second half of the test. Another way to compare grouped and solitary fish was to correlate the NFR for the parasitized compartment with elapsed time. No significant correlation was obtained for solitary fish (Spearman Rank Correlation: R S = -0.325, P = 0.303), while there was highly significant negative correlation for fish in groups (R S = -0.972, P < 0.0001). In fact, in tests with grouped fish, not a single fish was observed in the parasitized compartment after 150 minutes (Figure 1B).
Prevalence of infection was lower among fish in groups (66%) than in solitary fish (92%). Mean number of acquired metacercariae per fish was significantly lower when fish were in groups (mean ± SE: 10.8 ± 1.23 metacercariae per fish in grouped and 19.3 ± 3.27 in solitary fish) (Figure 2B) (Mann–Whitney U test: Z = 2.072; P = 0.038). Between-individual variation in the number of parasites per fish was noticeably higher across all fish tested in groups (coefficient of variation = 129%) than across all solitary fish (coefficient of variation = 81%).
Fish in groups and solitary fish were exposed to the same concentration of cercariae (240 cercariae l-1). By the end of exposure in Experiment 2, the total number of parasites acquired by all fish did not exceed 0.5% of the initial concentration in the water, even in the trials with fish groups. Such a small decrease in parasite concentration in the environment indicates the conditions of a non-depleted patch. Despite the different number of fish exposed, the estimated difference in cercariae concentration in “group” and “solitary” trials at the end of the exposure was only a negligible 0.3%.
The results of this study show that 1) fish can avoid parasitized habitats, and 2) fish in groups avoid parasitized habitats better than solitary fish. It might be assumed that grouped fish are more attractive for cercariae due to stronger stimuli (e.g. odour) released by fish in groups that could be used by parasites to locate hosts. However, our results indicate the opposite. Fish grouping behaviour reduces the risk of parasitism in environments where parasites are unevenly distributed. The results suggest that infected fish in groups, displaying an abnormal behaviour, transfer information about parasites. Other fish in the group could use this information and avoid a risky patch (the parasitized compartment of the experimental tank) better than solitary fish with no opportunity to observe conspecifics. This resulted in lower mean intensity of infection within the grouped fish compared to solitary fish in the present experiment. This effect could not be attributed to the different number of fish exposed (“dilution effect”). It has earlier been shown that the cercarial density, rather than fish density, affects parasite transmission to fish ; see also the calculation in the last paragraph of Results.
Between-individual variation in the number of acquired metacercariae was pronouncedly higher in the 'group’ treatment (across all fish tested) than in the 'solitary’ treatment. Grouped fish not only acquired fewer parasites than solitary fish, but some of the group members did not get any parasites. Within a group, most of the acquired parasites were aggregated in a few fish individuals. The most common explanation for uneven distribution of parasites among hosts is differences in innate resistance of individual fish to parasites [8, 33]. Our results provide new insight into this phenomenon. If differences in resistance would explain the individual differences in parasite intensity, then the between-individual variation should be more or less equal in experiments with solitary fish and fish in groups. We suggest that high variation in groups is related not only to individual differences in behaviour and physiology of fish, but also to information exchange within a group. Individuals within a group of fish are known to exchange information efficiently (reviewed in [11, 17]). Better avoidance of the parasitized compartments by fish in groups indicates that information about risky sites is somehow spread within the group.
How can the fish attacked by D. pseudospathaceum cercariae signal to the others about the danger? Abundance of infection was much higher in those fish groups which moved more actively. Higher motility per se could result in more frequent visits to the parasitized compartment in our experiment. In addition, higher motility can increase ventilation volume which, in turn, may facilitate transportation of D. pseudospathaceum cercariae to fish . Increased and conspicuous motility of fish injured by penetrating cercariae  and/or release of alarm substances  could be efficient signals. The most explorative fish would take a higher risk of infection while acquiring information about predators, food and surroundings. Less active fish within a group would benefit from avoiding risky activity and compensate for the information deficit by acquiring it from their more active conspecifics. As far as we know, this is the first empirical evidence suggesting transfer of information about infection risk within fish groups. Our results emphasize the role of behaviour as a factor affecting the between-individual variation in the number of acquired parasites.
Accumulation of parasites in a few fish could benefit the other members of a group, who would get a substantially lower infection or even avoid infection completely. A trade-off between faster exploration of a novel habitat and more cautious behaviour minimizing risks may contribute to within-group variability in acquired infection. Such a trade-off may depend on the “personality” of a host [35–37]. Another behaviourally-based mechanism stimulating aggregation of parasites on some host individuals was suggested by Poulin and co-authors . They found that prior infection increased the probability that a fish would acquire further parasites during a subsequent exposure. The authors suggested that this may be due to parasite-induced behavioural changes. If the individual behavioural differences rendering some fish more vulnerable to parasites are consistent, then this could contribute to the highly aggregated distribution of parasites among host individuals. Aggregated distributions are a common feature of most fish parasites, including D. spathaceum. The distribution of D. spathaceum is especially clumped in shoaling fish like Coregonus lavaretus, Osmerus eperlanus and Rutilis rutilus.
Avoidance of the parasitized compartment was much more pronounced in Experiment 2 where fish were allowed to explore the tank for 2 h prior to the tests. This time was required for habituation of fish to novel experimental conditions . During the acclimation period, fish habituate and explore novel surroundings [28, 40]. Exploration is an activity of high priority and could conflict with other vital activities [30, 31]. In Experiment 1, where fish were allowed to explore the experimental arena for only 15 minutes prior to testing, acclimation and exploration may have brought fish often into the parasitized compartment. The effect of pre-test exploration was more pronounced in fish groups than in solitary fish. This indicates more efficient functioning of fish in shoals while performing exploration of a novel habitat see also [11, 17].
The role of grouping behaviour of fish as a parasite-avoidance mechanism is much less studied than its role in anti-predator and foraging behaviour [11, 18]. Nevertheless, infection-associated changes in shoaling behaviour , avoidance of heavily infected fish within a group , and avoidance of risky microhabitats with large and easily recognizable parasites like crustacean ectoparasites  have received attention. Using the D. pseudospathaceum – O. mykiss model, we have shown that grouping also substantially facilitates avoidance of much smaller parasites like the tiny cercariae of trematodes.
In the present experiments, fish in groups acquired fewer parasites, so that almost half (44%) of the fish in groups were uninfected. By contrast, only 8% of the 'solitary’ fish were free of parasites. Highly uneven distribution of acquired parasites across the fish in groups may influence transmission of D. pseudospathaceum at the next step of its life cycle when the parasite is involved in food webs . Grouping seems to provide an efficient way to reduce intensity of parasitism for many members of the fish group. Thus, to minimize the risk of infection, fish may use not only costly physiological mechanisms, but also behavioural ones . Our results suggest that avoidance of high-parasite-risk habitats can be an important factor contributing to the evolution and maintenance of group behaviour in animals.
Our results show that fish can recognize parasitized areas and avoid them. This is especially important in heterogeneous habitats with patchily distributed, hard–to-detect parasites, like the cercariae of D. pseudospathaceum suspended in the water. Fish in groups recognize and avoid parasitized areas better than solitary fish. Fish in groups benefit from information about parasites in the environment gained from other members of a group. Grouped fish not only acquired fewer parasites than solitary fish, but some of the group members did not get any parasites. This suggests that grouping behaviour is an important mechanism of avoiding not only predators but also parasites. Fish use grouping, as well as individual behaviour and immune responses, to reduce the risk of parasitism. Variation in the number of parasites was much higher among fish in groups than among individually infected fish. We suggest that high variation in groups is related not only to individual differences in behaviour and physiology, but also to information exchange between members of a group. The most explorative fish would take a higher risk of infection while acquiring information about predators, food and surroundings. Less active fish within a group would benefit from avoiding risky activity and compensate for the lack of information by acquiring it from their more active conspecifics. If the individual differences in behaviour are consistent, this could contribute to increased variation in the number of parasites among host individuals. Our results support the idea that parasitism may be an important factor contributing to the evolution and maintenance of group behaviour in fish.
Konnevesi Research Station provided the facilities and necessary assistance for the experiments. We thank Dr. A. Karvonen for providing infected snails. The study was supported by the Mobility Grant of the Academy of Finland (grants to ETV in 2006 and to JT in 2010), RFBR (grants 11-04-00136 to VM and 13-04-00613 to AP). Thanks are due to the two anonymous reviewers for valuable comments and suggestions. We are grateful to Prof. R. Jones and Dr. D. Benesh who kindly checked the English.
- Price PE: Evolutionary biology of parasites. 1980, Princeton University Press: PrincetonGoogle Scholar
- Poulin R: Evolutionary ecology of parasites. From individuals to communities. 1998, London: Chapman & HallGoogle Scholar
- Schmid-Hempel P: Parasites in social insects. 1998, Princeton University Press: PrincetonGoogle Scholar
- Moore J: Parasites and the behavior of animals. 2002, Oxford: Oxford University PressGoogle Scholar
- Hart BL: Behavioral adaptations to pathogenes and parasites: five strategies. Neurosci Biobehav Rev. 1990, 14: 273-294. 10.1016/S0149-7634(05)80038-7.View ArticlePubMedGoogle Scholar
- Hart BL: Behavioral adaptations to parasites: an ethological approach. J Parasitol. 1992, 78: 256-265. 10.2307/3283472.View ArticlePubMedGoogle Scholar
- Moller AP, Dufva R, Allander K: Parasites and the evolution of host social behavior. Adv Study Behav. 1993, 22: 65-102.View ArticleGoogle Scholar
- Karvonen A, Seppälä O, Valtonen ET: Parasite resistance and avoidance behaviour in preventing eye fluke infections in fish. Parasitology. 2004, 129: 159-164. 10.1017/S0031182004005505.View ArticlePubMedGoogle Scholar
- Altizer S, Nunn CL, Thrall PH, Gittleman JL, Antonovics J, Cunningham AA, Dobson AP, Ezenwa V, Jones KE, Pedersen AB, Poss M, Pullian JRC: Social organization and parasite risk in mammals: integrating theory and empirical studies. Annl Rev Ecol Syst. 2003, 34: 517-547. 10.1146/annurev.ecolsys.34.030102.151725.View ArticleGoogle Scholar
- Poulin R, FitzGerald GJ: Shoaling as an anti-ectoparasite mechanism in juvenile sticklebacks (Gasterosteus spp.). Behav Ecol Sociobiol. 1989, 24: 251-255. 10.1007/BF00295205.View ArticleGoogle Scholar
- Krause J, Ruxton GD: Living in groups. 2002, Oxford: Oxford University PressGoogle Scholar
- Poulin R, FitzGerald GJ: Risk of parasitism and microhabitat selection in juvenile sticklebacks. Can J Zool. 1989, 67: 14-18. 10.1139/z89-003.View ArticleGoogle Scholar
- Laitinen M, Siddall R, Valtonen ET: Bioelectronic monitoring of parasite-induced stress in brown trout and roach. J Fish Biol. 1996, 48: 228-241. 10.1111/j.1095-8649.1996.tb01115.x.View ArticleGoogle Scholar
- Poulin R, Marcogliese DJ, McLaughlin JD: Skin-penetrating parasites and the release of alarm substances in juvenile rainbow trout. J Fish Biol. 1999, 55: 47-53. 10.1111/j.1095-8649.1999.tb00655.x.View ArticleGoogle Scholar
- Karvonen A, Seppälä O, Valtonen ET: Eye fluke-induced cataract formation in fish: quantitative analysis using an ophtalmological microscope. Parasitology. 2004, 129: 473-478. 10.1017/S0031182004006006.View ArticlePubMedGoogle Scholar
- Mikheev VN, Pasternak AF, Taskinen J, Valtonen ET: Parasite-induced aggression and impaired contest ability in a fish host. Parasit Vectors. 2010, 3: 17-10.1186/1756-3305-3-17.PubMed CentralView ArticlePubMedGoogle Scholar
- Pitcher TJ, Parrish JK: Functions of shoaling behaviour in teleosts. Behaviour of Teleost Fishes. Edited by: Pitcher TJ. 1993, London: Chapman and Hall, 364-439. 2View ArticleGoogle Scholar
- Barber I, Hoare D, Krause J: Effects of parasites on fish behaviour: a review and evolutionary perspective. Rev Fish Biol Fisheries. 2000, 10: 131-165. 10.1023/A:1016658224470.View ArticleGoogle Scholar
- Sweeting RA: Investigations into natural and experimental infections of freshwater fish by the common eye-fluke Diplostomun spathaceum Rud. Parasitology. 1974, 69: 291-300. 10.1017/S0031182000062995.View ArticlePubMedGoogle Scholar
- Côté IM, Poulin R: Parasitism and group size in social animals: a meta-analysis. Behav Ecol. 1995, 6: 159-165. 10.1093/beheco/6.2.159.View ArticleGoogle Scholar
- Magurran AE, Higham A: Information transfer across fish shoals under threat. Ethology. 1988, 78: 153-158.View ArticleGoogle Scholar
- Louhi K-R, Karvonen A, Rellstab C, Jokela J: Is the population genetic structure of complex life cycle parasites determined by the geographic range of the most motile host?. Infect Genet Evol. 2010, 10: 1271-1277. 10.1016/j.meegid.2010.08.013.View ArticlePubMedGoogle Scholar
- Rellstab C, Louhi K-R, Karvonen A, Jokela J: Analysis of trematode parasite communities in fish eye lenses by pyrosequencing of naturally pooled DNA. Infect Genet Evol. 2011, 11: 1276-1286. 10.1016/j.meegid.2011.04.018.View ArticlePubMedGoogle Scholar
- Karvonen A, Paukku S, Valtonen ET, Hudson PJ: Transmission, infectivity and survival of Diplostomum spathaceum cercariae. Parasitology. 2003, 127: 217-224. 10.1017/S0031182003003561.View ArticlePubMedGoogle Scholar
- Haas W: Physiological analysis of host-finding behaviour and trematode cercariae: adaptations for transmission success. Parasitology. 1994, 109: S15-S29. 10.1017/S003118200008505X.View ArticlePubMedGoogle Scholar
- Chappell LH: The parasites of the three-spined stickleback Gasterosteus aculeatus L. from a Yorkshire pond. II. Variation of the parasite fauna with sex and size of fish. J Fish Biol. 1969, 1: 339-347. 10.1111/j.1095-8649.1969.tb03881.x.View ArticleGoogle Scholar
- Wootten R: Observations on strigeid metacercariae in the eyes of fish from Hanningfield Reservoir, Essex, England. J Helminthol. 1974, 48: 73-83. 10.1017/S0022149X00022628.View ArticlePubMedGoogle Scholar
- Kleerekoper H, Matis J, Gensler P, Maynard P: Exploratory behaviour of goldfish Carassius auratus. Anim Behav. 1974, 22: 124-132. 10.1016/S0003-3472(74)80061-8.View ArticleGoogle Scholar
- Mikheev VN, Andreev OA: Two-phase exploration of a novel environment in the guppy, Poecilia reticulata. J Fish Biol. 1993, 42: 375-383. 10.1111/j.1095-8649.1993.tb00340.x.View ArticleGoogle Scholar
- Barnet SA, Cowan PE: Activity, exploration, curiosity and fear: an ethological study. Interdisc Sci Rev. 1976, 1: 43-62. 10.1179/030801876789768534.View ArticleGoogle Scholar
- Stamps J: Motor learning and the value of familiar space. Am Nat. 1995, 146: 41-58. 10.1086/285786.View ArticleGoogle Scholar
- Höglund J: Experiments on second intermediate fish host related cercarial transmission of the eyefluke Diplostomum spathaceum into rainbow trout (Oncorhynchus mykiss). Folia Parasitol (Praha). 1995, 42: 49-53.Google Scholar
- Pennycuick L: Differences in the parasite infections in three-spined sticklebacks (Gasterosteus aculeatus L.) of different sex, age and size. Parasitology. 1971, 63: 407-418. 10.1017/S0031182000079932.View ArticlePubMedGoogle Scholar
- Höglund J: Ultrastructural observations and radiometric assay on cercarial penetration and migration of the digenean Diplostomum spathaceum in the rainbow trout Oncorhynchus mykiss. Parasitol Res. 1991, 77: 283-289. 10.1007/BF00930902.View ArticlePubMedGoogle Scholar
- Kortet R, Hedrick AV, Vainikka A: Parasitism, predation and the evolution of animal personalitis. Ecol Lett. 2010, 13: 1449-1458. 10.1111/j.1461-0248.2010.01536.x.View ArticlePubMedGoogle Scholar
- Dall SRX, Houston AI, McNamara JM: The behavioural ecology of personality: consistent individual differences from an adaptive perspective. Ecol Lett. 2004, 7: 734-739. 10.1111/j.1461-0248.2004.00618.x.View ArticleGoogle Scholar
- Sih A, Bell MA, Johnson JC, Ziemba RE: Behavioral syndromes: an integrative overview. Quart Rev Biol. 2004, 79: 241-277. 10.1086/422893.View ArticlePubMedGoogle Scholar
- Poulin R, Rau ME, Curtis MA: Infection of brook trout fry, Salvelinus fontinalis, by ectoparasitic copepods: the role of host behaviour and initial parasite load. Anim Behav. 1991, 41: 467-476. 10.1016/S0003-3472(05)80849-8.View ArticleGoogle Scholar
- Seppälä O, Karvonen A, Valtonen ET: Eye fluke-induced cataracts in natural fish populations: is there potential for host manipulation?. Parasitology. 2011, 138: 209-214. 10.1017/S0031182010001228.View ArticlePubMedGoogle Scholar
- Teyke T: Learning and remembering the environment in the blind cave fish Anoptichthys jordani. J Compar Physiol A. 1989, 164: 655-662. 10.1007/BF00614508.View ArticleGoogle Scholar
- Barber I, Huntingford FA: The effect of Schistocephalus solidus (Cestoda: Pseudophyllidea) on the foraging and shoaling behaviour of three-spined sticklebacks, Gasterosteus aculeatus. Behaviour. 1995, 132: 1223-1240. 10.1163/156853995X00540.View ArticleGoogle Scholar
- Krause J, Godin JGJ: Influence of parasitism on shoal choice in the bunded killifish (Fundulus diaphanous, Teleostei, Cyprinodontidae). Ethology. 1996, 102: 40-49.View ArticleGoogle Scholar
- Sukhdeo MVK: Where are the parasites in food webs?. Parasit Vectors. 2012, 5: 239-10.1186/1756-3305-5-239.PubMed CentralView ArticlePubMedGoogle Scholar
- Hart BL: Behavioural defense against parasites: interaction with parasite invasiveness. Parasitology. 1994, 109: S139-S151. 10.1017/S0031182000085140.View ArticlePubMedGoogle Scholar
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