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Table 1 Measures (μm) of microfilariae determined after fixation with the modified Knott’s technique

From: Morphometric analyses of canine blood microfilariae isolated by the Knott’s test enables Dirofilaria immitis and D. repens species-specific and Acanthocheilonema (syn. Dipetalonema) genus-specific diagnosis

Microfilariae species identified by histochemical staining and partially confirmed by PCRa Total number and origin of dogs Mean length ± standard deviation (μm) Mean width ± standard deviation (μm)
Dirofilaria immitis 60 (GR 1; IT 6; PT 33; ES 16; TR 2; UO 2) 301.77 ± 6.29 6.30 ± 0.26
Dirofilaria repens 171 (BG 1; FR 1; GR 5; IT 10; RO 3; ES 2; HU 141; UO 8) 369.44 ± 10.76 8.87 ± 0.58
Acanthocheilonema dracunculoides 133 (PT 69; ES 55; UO 9) 259.43 ± 6.69 5.09 ± 0.47
Acanthocheilonema reconditum 13 (IT 13) 264.83 ± 5.47 4.63 ± 0.52
  1. Mean length and mean width (μm) of microfilariae determined after fixation with the modified Knott’s technique of confirmed single filarial infections in 377 dogs.
  2. IT, Italy; ES, Spain; GR, Greece; PT, Portugal; RO, Romania; HU, Hungary; BG, Bulgaria; TR, Turkey; FR, France; Unknown Origin: UO.
  3. a PCR confirmation for 6 samples positive for D. immitis, 4 for D. repens, 7 for A. dracunculoides and 6 for A. reconditum infections.