Prevalence and risk factors of Strongyloides stercoralis in Takeo Province, Cambodia
© Khieu et al.; licensee BioMed Central Ltd. 2014
Received: 24 November 2013
Accepted: 28 April 2014
Published: 12 May 2014
The threadworm Strongyloides stercoralis, the most neglected helminth, affects an estimated 30-100 million people worldwide. Information on S. stercoralis infection is scarce in tropical and sub-tropical resource poor countries, including Cambodia. We determined S. stercoralis infection prevalence and risk factors for infection in the general population in Southern Cambodia.
A cross-sectional study was carried out between January and April 2011 among 2,861 participants living in 60 villages of Takeo province, using Koga-agar plate culture, the Baermann technique and the Kato-Katz technique on a single stool sample.
Eight intestinal helminth species were diagnosed. Hookworm (31.4%) and S. stercoralis (21.0%) occurred most frequently. Prevalence of S. stercoralis infection increased with age. In all age groups a higher prevalence was found among males than among females (OR: 1.7; 95% CI: 1.4 – 2.0; P < 0.001). Participants who had a latrine at home were significantly less frequently infected with S. stercoralis than those who did not (OR: 0.7; 95% CI: 0.4 – 0.8; P = 0.003). Muscle pain (OR: 1.3; 95% CI: 1.0 – 1.6; P = 0.028) and urticaria (OR: 1.4; 95% CI: 1.1 – 1.8; P = 0.001) were significantly associated with S. stercoralis infection.
S. stercoralis is highly prevalent among the general Cambodian population and should no longer be neglected. Access to adequate diagnosis and treatment is urgently needed.
KeywordsStrongyloides stercoralis Prevalence Risk factors Clinical manifestation Cambodia
Strongyloides stercoralis, a soil-transmitted nematode, is arguably the most neglected tropical disease , yet an estimated 30 – 100 million people are infected worldwide . Despite its frequency, epidemiological data on prevalence and geographical variations are largely lacking . The prevalence of S. stercoralis is often underestimated, as most diagnostic methods used have a low sensitivity for S. stercoralis[4–6]. Furthermore, in resource poor countries, environmental conditions and poor hygiene behaviour favour transmission. For Cambodia, only a few reports are available, giving prevalences ranging from 2.6% to 24.4% [6–10].
To date, there is no universally agreed upon gold standard for diagnosing S. stercoralis. Molecular and serological methods have been reported as promising diagnostic tools, yet these techniques require further validation or further development . Therefore, definitive diagnosis relies on the detection of larvae in stool specimens . The Baermann technique  and Koga-agar plate (KAP) culture  are the coprological diagnostic methods most often employed. The sensitivity and specificity of either technique alone is not satisfactory [6, 14–16]. Thus, to increase the likelihood of detecting S. stercoralis, combined use of these diagnostic tests has been suggested .
S. stercoralis infection is acquired by infective filariform larvae (L3), originating from contaminated soil , directly penetrating the skin. The clinical presentation of strongyloidiasis is extremely variable, ranging from asymptomatic patients to patients with gastrointestinal symptoms (e.g., abdominal pain, diarrhoea) and urticaria , to disseminated infections with mortality rates as high as 87% .
The present cross-sectional study aimed to determine the prevalence, risk factors and associated clinical manifestations of S. stercoralis infection in a random population sample living throughout 60 villages of Takeo province, southern Cambodia.
The study was approved by the National Ethics Committee for Health Research, Ministry of Health of Cambodia (NECHR # 185, dated 20 December 2010) and by the Ethics Committee of the Cantons of Basel-Stadt and Baselland, Switzerland (EKBB #14/11, 13 January 2011). All participants were informed in Khmer language about the purpose and procedures of the study. Written informed consent was obtained from all participants prior to enrolment. For participants aged 1 to 18 years, consent was obtained from the parents, legal guardian or appropriate literate substitutes.
All participants infected with S. stercoralis were treated with a single oral dose of ivermectin (200 μg/kg body weight) . All other parasitic infections were treated according to the guidelines of the National Helminth Control Program of Cambodia .
Study design, area and population
Takeo province, with its expansive floodplains, is located in the South of Cambodia, about 80 kilometres from the capital, Phnom Penh, along the border with Vietnam. Takeo has 843,931 inhabitants, with a density of 237 people per square kilometre. The economy is based on agriculture, namely fisheries, rice and fruit cropping. Pigs, dogs, cats, poultry and cattle are the most common domestic animals . No information on the prevalence of S. stercoralis is available for Takeo province. However, the prevalences of intestinal helminth infections have recently been reported in Ang Svay Chek villages, Prey Kabas district, and were 13.2% for hookworm and 47.5% for Opisthorchis viverrini and minute intestinal flukes together .
Fifteen households were randomly selected from each village. All household members, 12 months or older, were eligible and informed about the study. All household members present on the day of the survey were enrolled.
A pre-tested household questionnaire was administered to the head of household to obtain information on socioeconomic indicators such as house type (concrete, wood or leaves), household assets (sewing machine, television, radio, motorcycle or car), livestock (cattle, pigs or poultry) and availability of a latrine at home. Participants were interviewed based on a pre-tested individual questionnaire in order to collect information about demographics (age, gender, educational level and profession), personal risk-perception (knowledge about worm infections) personal hygiene practices (washing hands after defecating or before eating), wearing shoes (at home/work) and use of latrines for defecation. All questionnaires were developed in English and translated into Khmer. A different person translated the questionnaires back into English to ensure correct content. The interviews were conducted in Khmer language. After the interview, each participant received a pre-labelled plastic container (ID code, name, sex, age and date) for collecting a stool sample the next morning. Within two hours of collection, the stool specimens were sent (at ambient temperature) to the laboratory in Takeo town, where they were immediately processed.
First, two Kato-Katz thick smears were prepared, using the World Health Organization’s (WHO’s) standard template . After a clearance time of 30 minutes, the smears were examined under a light microscope for the presence of helminth eggs. These were counted and recorded for each helminth species separately. Second, the KAP culture was used to identify S. stercoralis and hookworm larvae . A hazelnut-sized stool sample was placed in the middle of the agar plate and the closed Petri dish was incubated in a humid chamber for 48 hours at 28°C. Then, the plate was rinsed with sodium acetate-acetic acid-formalin (SAF) solution. The eluent was centrifuged and the sediment microscopically examined for the presence of S. stercoralis and hookworm larvae. Finally, the Baermann technique was performed . A walnut-sized stool sample was placed on gauze inserted into a glass funnel, and covered with tap water. The apparatus was exposed for two hours to artificial light directed from below. After centrifugation of the collected liquid, the sediment was examined under a microscope for the presence of S. stercoralis larvae.
For quality control, the technicians were specifically trained on the morphological criteria for distinguishing hookworm and S. stercoralis larvae. During the entire study period, a qualified microscopist from the Swiss Tropical and Public Health Institute, Basel, Switzerland, provided continuous and rigorous supervision. Further, any unclear diagnosis was immediately discussed with a qualified microscopist and the study supervisor.
Data management and statistical analyses
Questionnaire and laboratory data collected from each participant was double-entered and validated in EpiData version 3.1 (EpiData Association; Odense, Denmark). Statistical analyses were performed with STATA version 12.1 (StataCorp.; College Station, TX, USA). Only participants with complete records (stool sample examined with all diagnostic methods and completed questionnaires) were included in the final analysis. A ‘smoothed’ age prevalence curve was used to present the prevalence distribution of the mean age of each participant.
Principle component analysis (PCA) was applied to variables pertaining to ownership of various household assets, and was used to build the socioeconomic status (SES) profile. SES was categorized by one of three wealth levels: least poor, less poor and poor, as previously described in detail .
Generalized Estimating Equations (GEE) were used to determine the association between infection status and demographic variables, hygienic status, knowledge and the recent medical history of participants. Variables with an odds ratio below 0.80 and above 1.25 in the bivariate models were selected for inclusion in the multivariate GEE model.
Study population and compliance
In total, 3,568 individuals from 900 households (median household size: 5 members, range: 1 – 12) were enrolled, of which 3,154 (88.4%) submitted a stool sample. The final analysis included 2,861 (80.2%) participants with complete data records, i.e., enough stool for all three diagnostic tests (two Kato-Katz, KAP culture and Baermann method) and all questionnaire data.
Of the 2,861 participants with complete data records, 1,560 (54.5%) were female. The median age of all participants was 26 years, with a range from 1 to 90 years and 57.2% aged 30 years or under. One third or 31.7% were schoolchildren; 40.5% were farmers; three-quarters or 74.7% had attended school, with 50.5% and 24.2% attending primary and secondary school or higher, respectively.
Parasitological findings and performance of diagnostic methods
Yield of Koga-agar culture and Baermann method for detecting S. stercoralis in 2,861 participants, Takeo province, Cambodia
Combined Methods (KAP culture and Baermann)
Risk factors for Strongyloides stercoralis infection
Risk factors for S . stercoralis infection among 2,861 participants, Takeo province, Cambodia
Non-S. stercoralis(N = 2260) n (%)
S. stercoralis(N = 601) n (%)
OR (95% CI)
1.7 (1.4 – 2.0)
1 - 15 years
16 - 30 years
1.2 (0.8 - 1.7)
31 - 45 years
1.3 (0.9 - 1.9)
> 46 years
1.3 (0.9 - 2.0)
0.7 (0.5 - 1.1)
0.7 (0.5 - 0.9)
0.8 (0.6 - 1.0)
0.9 (0.7 - 1.2)
Personal disease perception
Have been treated for worms (yes)
0.8 (0.7 - 1.0)
Know about worms/infection with worms (yes)
1.0 (0.8 - 1.2)
Toilet at home (yes)
0.7 (0.4 - 0.8)
Usually defecated in toilet (yes)
1.2 (0.8 - 1.7)
Had shoes (yes)
1.1 (0.7 - 1.9)
Wore shoes when going to defecate/toilet (yes)
1.1 (0.7 - 1.6)
Characteristics of Strongyloides stercoralis cases
Of the 601 S. stercoralis cases, 332 (55.2%) were male and 308 (51.3%) were farmers. Almost one-third (28.0%) of the cases were children under 16 years, while 256 (42.6%) were between the ages of 16 and 45 years. A quarter (25.6%) of the cases occurred among schoolchildren (Table 2).
Clinical symptoms for S. stercoralis cases in 2,861 participants, Takeo province, Cambodia, 2011
Symptoms mentioned in the past 2 weeks
Non-S. stercoralis(N = 2260) n (%)
S. stercoralis(N = 601) n (%)
OR (95% CI)
Anorexic and/or loss of appetite (yes)
1.0 (0.8 - 1.3)
Sensation of tiredness (yes)
1.1 (0.9- 1.3)
1.0 (0.8 - 1.3)
0.8 (0.6 - 1.1)
1.0 (0.9 - 1.3)
Bloody diarrhoea (yes)
1.0 (0.6 - 1.7)
Greasy diarrhoea (yes)
1.0 (0.7 - 1.4)
0.8 (0.6 - 1.0)
1.1 (0.9 - 1.3)
Seen worm in stool (yes)
0.8 (0.6 - 1.1)
1.1 (0.9 - 1.3)
Coughed out a worm (yes)
0.7 (0.2 - 3.0)
Cutaneous rash “urticaria” (yes)
1.4 (1.1 - 1.8)
Abdominal pain (yes)
0.9 (0.8 - 1.1)
Muscle pain (yes)
1.3 (1.0 - 1.6)
Lost weight (yes)
1.1 (0.8 - 1.4)
The worldwide distribution of S. stercoralis infection varies greatly from country to country and even within the same country, depending on ecological and socioeconomic conditions . S. stercoralis infection is commonly overlooked, especially in endemic resource poor countries, as the most suitable diagnostic methods for S. stercoralis (KAP culture and Baermann technique) are not used in most epidemiological studies of soil-transmitted helminths [1, 5]. Despite the high endemicity of S. stercoralis in Southeast Asia, specific information is often lacking. Thailand is the only country in the region with a considerable amount of information available on S. stercoralis prevalence . Our community-based study of S. stercoralis infection among 2,861 participants from 60 villages in a southern province of Cambodia, employing KAP culture and the Baermann method, found a prevalence of 21.0%.
To our knowledge, this is the first community-based report in Cambodia on the importance of S. stercoralis infection in a large province-wide setting, using a rigorous diagnostic technique (KAP culture and the Baermann technique). Recent surveys on S. stercoralis infection in Cambodia studied mainly the diagnosis, treatment and risk factors of infection among schoolchildren , the clinical aspects of high intensity infection  and the molecular diagnostic approach (PCR) . Our report highlights the high frequency of the parasite in the general population, and points to key risk factors, which need to be addressed in large-scale control programmes (country-wide).
Our observed prevalence is particularly high compared to those of two previous studies that used the Baermann technique on samples from school-aged children (12.0%) and from the general population in Cambodia (14.6%) [8, 9]. One possible explanation is that the present study used a combined diagnostic method (KAP culture and Baermann technique) to diagnose S. stercoralis, while others used a single test. However, a similar prevalence rate (20.2%) was observed in a 2006 study that had only used the Baermann technique to analyse a single stool sample from school-aged children living in villages bordering Tonlé Sap Lake, central-northern Cambodia . Moreover, a recent study among schoolchildren in Kandal province, central-southern Cambodia, which used the same laboratory approach on multiple stool samples, reported a prevalence of 24.4% , which is consistent with our findings when using the same diagnostic methods on a single stool sample. This observation suggests that the overall prevalence of S. stercoralis in our study population would actually have been higher if several stool samples had been examined.
The prevalence of S. stercoralis in our study setting is higher than those shown in other studies among the general population in Southeast Asian countries: Lao PDR, Thailand and China. In Lao PDR, the study conducted by Sayasone et al. in 2009, using a formalin ethyl-acetate concentration technique on a single stool sample, found that 10.3% of participants were infected with S. stercoralis, but this diagnostic approach is not very sensitive . In Thailand, Nontasut et al. used KAP culture to analyse a single stool sample and reported a prevalence of 15.9% . In China, Steinmann et al. examined three stool samples by KAP culture and Baermann method and found a prevalence of 11.7% .
In our study, the observed prevalence of S. stercoralis was significantly higher in males than in females, which coincides with previous reports from Thailand  and China . In addition, males had a higher infection rate than females in all age groups. This is likely due to agricultural practices and activities. Most men are rice farmers and work in muddy rice fields without footwear. In contrast, Cambodian females usually work as housewives and wear shoes when walking around the household or village. The prevalence of S. stercoralis also increased with age, starting from 14.5% in children 5 years and under, to a peak prevalence of 28.0% in participants aged 56 to 60, decreasing slightly thereafter. This suggests that infection first occurs in the village or household where young children play, usually without shoes. After contracting the parasite at a young age, the infection may remain for decades in the host if the infection is not treated [28–30], which would account for the increased prevalence associated with age.
The clinical manifestations of strongyloidiasis vary greatly between immune-competent and immune-suppressed individuals. Gastrointestinal (nausea and diarrhea) and cutaneous (itchiness and urticaria) symptoms are frequently described. However, more than 50% of strongyloidiasis cases are asymptomatic [18, 19, 31, 32]. In our study, we observed that muscle pain and urticaria reported during the preceding two weeks were associated with S. stercoralis infection; a finding that coincides with recent reports from the Northern province of Cambodia . The clinical features of strongyloidiasis and the association between strongyloidiasis and other infectious diseases are not well understood [19, 31]. With the limitations of our study, it was not possible to differentiate underlying infections that might mimic strongyloidiasis. Therefore, an indepth assessment on the clinical symptoms of S. stercoralis infection is required.
The sensitivity of KAP culture and the Baermann technique has been reviewed in several studies, with contradictory results. Our study found the sensitivity of KAP culture to be higher than that of the Baermann method, contrary to previous reports from south-central Côte d’Ivoire , Zanzibar , Uganda  and China . However, our findings are consistent with those reported in studies conducted in Cambodia , Brazil , rural Côte d’Ivoire  and Honduras .
Our study examined only a single stool sample per participant, thus the prevalence of S. stercoralis in our study setting was likely under-estimated, since the excretion of S. stercoralis larvae in stool specimens varies considerably from day-to-day [34, 37]. A recent study showed that the output of larvae in faecal specimens ranged from 0.003 larvae per gram to 151.2 larvae per gram, as observed over seven consecutive days . However, even when multiple stool samples are available, no single diagnostic test, KAP culture or Baermann method, can detect all S. stercoralis infection [6, 34]. Therefore, the combined use of both diagnostic methods (KAP culture and Baermann technique) on several stool specimens will increase sensitivity and currently represents the best diagnostic approach for this infection.
S. stercoralis infection is highly prevalent among the general population in Cambodia and should be given more attention due to its potential for disseminating infection. Access to adequate diagnosis and treatment is urgently required in Cambodia.
We are grateful to the participants and local authorities of Takeo province. We deeply thank the laboratory technicians from the Helminth Control Program of the National Centre for Parasitology, Entomology and Malaria Control for their valuable laboratory work, and the staff from Takeo Provincial Health Department for their great field work. The study was supported by the UBS Optimus Foundation, Zürich, Switzerland. The authors thank Mrs. Amena Briet for her efficient English editing.
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