Bronchopulmonary lophomoniasis: emerging disease or unsubstantiated legend?
© Martinez-Girón and van Woerden; licensee BioMed Central Ltd. 2014
Received: 22 May 2014
Accepted: 19 June 2014
Published: 23 June 2014
The relationship between Lophomonas, a genus of multiflagellated protozoa, and respiratory pathology has recently received attention. Here, we summarize the origin, mode of transmission, pathogenic mechanism and relevant clinical data of bronchopulmonary lophomoniasis.
KeywordsBronchopulmonary lophomoniasis Lophomonas blattarum Cockroaches
The relationship between Lophomonas, a genus of multiflagellated protozoa, and respiratory pathology has recently received attention in a Chinese article by Mu et al.  which concludes that, “In the past 20 years, all the diagnosed cases as pulmonary Lophomonas blattarum infection reported in our country were misdiagnosed. Currently, there is no evidence to show Lophomonas blattarum as a pathogen resulting in pulmonary infection.” This conclusion is at odds with a literature review in which we identified 61 case reports of pulmonary L. blattarum.
We recognise that it is possible for “detached ciliary tufts” (also described as “Ciliocytophthoria” phenomena) to be confused with protozoa in sputum samples. Cytoplasmic remnants with cilia are a frequent finding in the bronchial secretions from patients with obstructive pulmonary disease and asthma  Nevertheless, parasitic infection caused by the multiflagalled protozoon L. blattarum has been described , and its presence in respiratory secretions, in both fresh and stained smears, has been observed .
It is well recognised that certain protozoa are capable of forming protective cysts to survive in difficult conditions. The encystment of L. blattarum has been illustrated in detail in stained preparations by Kudo . It is reasonable to hypothesise that viable cysts of L. blattarum could enter into the human body by inhalation or ingestion of material contaminated by cockroach faeces. Once in the bronchial tree, and under favourable conditions of temperature and humidity, excystation would logically be expected. Thus, and as it happens with other types of human enteric protozoa such as Entamoeba histolytica, Balantidium coli, Toxoplasma gondii, Cryptosporidium parvum and Cyclospora cayetanensis[9, 10], the cockroaches may also be regarded as vectors for this multiflagellated protozoon.
As regard its pathogenic mechanism, it is well-known that certain strong protozoan proteases are involved in different mechanisms of cellular damage such as cytoadherence, breakdown of epithelial barriers, induction to apoptosis, etc. Recently, Acanthamoeba protease activity has been reported to have induced allergic airway inflammation in mice via PARs-2 . In a similar context, L. blattarum could damage the respiratory epithelium.
A spectrum of clinical manifestations of infestation with L. blattarum can be described, ranging from mild cough and slight wheezing to severe respiratory insufficiency with purulent exudates, high fever and radiological signs of pulmonary consolidation. The diagnosis may be confirmed by observing living protozoal forms in fresh samples from respiratory secretions (sputa, bronchial aspirates and bronchoalveolar lavages), where the characteristic irregular movement of flagella can be observed. Alternatively, identification can be undertaken by the staining of smears using Mallory’s trichromic stain or a combination of Papanicolaou and Giemsa stains.
Metronidazole and its derivatives have been utilized for the treatment of this pulmonary infestation in a number of case reports with satisfactory outcomes.
We believe that the observation under light microscopy of this multiflagellated protozoon in symptomatic patients, who respond positively to antiprotozoal therapy, can reasonably be described as bronchopulmonary lophomoniasis. It is possible that the organism exists as a commensal in some individuals and may only be pathogenic in the context of a causal web of aetiological factors . It is also possible that more than one species is being observed which has similar features under light microscopy. However, we believe that this infection should be recognised as a potentially important emerging field of study within respiratory medicine. The development of a technique to culture the organism or the use of molecular techniques is required to resolve the issue.
The authors thank Mr. Juan Galán, secretary of the INCLÍNICA foundation for his technical assistance.
- Mu XL, Shang Y, Zheng SY, Zhou B, Yu B, Dong XS, Cao ZL, Jiang N, Sun KK, Chen YC, Xi W, Gao ZC: A study on the differential diagnosis of ciliated epithelial cells from Lophomonas blattarum in bronchoalveolar lavage fluid. Zhonghua Jie He He Hu Xi Za Zhi. 2013, 36: 646-650. [Article in Chinese]PubMedGoogle Scholar
- Martinez-Girón R, van Woerden HC: Lophomona blattarum and bronchopulmonary disease. J Med Microbiol. 2013, 62: 1641-1648. 10.1099/jmm.0.059311-0.View ArticlePubMedGoogle Scholar
- Martínez-Girón R, Van Woerden HC, Doganci L: Lophomonas misidentification in bronchoalveolar lavages. Intern Med. 2011, 50: 2721-10.2169/internalmedicine.50.5878. author reply 2723View ArticlePubMedGoogle Scholar
- Vijayan VK, Kilani T: Emerging and established parasitic lung infestations. Infect Dis Clin North Am. 2010, 24: 579-602. 10.1016/j.idc.2010.04.002.View ArticlePubMedGoogle Scholar
- Martínez-Girón R: Protozoal infections. Parasitic diseases of the lungs. Edited by: Barrios R, Haque AK. 2013, New York: Springer-Verlag, 47-68.View ArticleGoogle Scholar
- Gile GH, Slamovits CH: Phylogenetic position of Lophomonas striata Bütschli (Parabasalia) from the hindgut of the cockroach Periplaneta americana. Protist. 2012, 163: 274-283. 10.1016/j.protis.2011.07.002.View ArticlePubMedGoogle Scholar
- Brugerolle G, Lee JJ: Phylum Parabasalia. An Illustrated Guide to the Protozoa. Edited by: Lee JJ, Leedale GF, Bradbury P. 2000, Lawrence: Society of Protozoologists, 1196-1250. 2, 2Google Scholar
- Kudo RR: Handbook of Protozoology. 1931, Springfield, Illinois: Charles C Thomas, 8-9.Google Scholar
- Graczyk TK, Knight R, Tamang L: Mechanical transmission of human protozoan parasites by insects. Clin Microbiol Rev. 2005, 18: 128-132. 10.1128/CMR.18.1.128-132.2005.PubMed CentralView ArticlePubMedGoogle Scholar
- Tatfeng YM, Usuanlele MU, Orukpe A, Digban AK, Okodua M, Oviasogie F, Turay AA: Mechanical transmission of pathogenic organisms: the role of cockroaches. J Vector Borne Dis. 2005, 42: 129-134.PubMedGoogle Scholar
- Park MK, Cho MK, Kang SA, Park HK, Kim DH, Yu HS: Acanthamoeba protease activity promotes allergic airway inflammation via protease-activated receptor 2. PLoS One. 2014, 9: e92726-10.1371/journal.pone.0092726.PubMed CentralView ArticlePubMedGoogle Scholar
- van Woerden HC, Ratier-Cruz A, Aleshinloye OB, Martinez-Giron R, Gregory C, Matthews IP: Association between protozoa in sputum and asthma: a case–control study. Respir Med. 2011, 105: 877-884. 10.1016/j.rmed.2010.11.016.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.