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Table 2 Hybridization experiments between isolines of An. sinensis and An. kleini

From: Evidence to support natural hybridization between Anopheles sinensis and Anopheles kleini (Diptera: Culicidae): possibly a significant mechanism for gene introgression in sympatric populations

Crosses (Female x Male) Total eggs (number)* Embryonation rate Hatched n (%) Pupation n (%) Emergence n (%) Total emergence n (%)
       Female Male
Parental crosses        
An. sinensis x An. sinensis 538 (258, 280) 96 498 (92.57) 463 (92.97) 449 (96.98) 211 (46.99) 238 (53.01)
An. kleini x An. kleini 469 (263, 206) 89 413 (88.06) 322 (77.96) 303 (94.10) 136 (44.88) 167 (55.12)
Reciprocal crosses        
An. sinensis x An. kleini 529 (288, 241) 86 450 (85.07) 441 (98.00) 436 (98.87) 253 (58.03) 183 (41.97)††
An. kleini x An. sinensis 422 (239, 183) 83 350 (82.94) 350 (100.00) 350 (100.00) 178 (50.86) 172 (49.14)††
Back crosses        
(An. sinensis x An. kleini)F1 x An. sinensis 401 (218, 183) 63 245 (61.10) 245 (100.00) 238 (97.10) 140 (58.82) 98 (41.18)
(An. sinensis x An. kleini)F2 x An. sinensis 397 (207, 190) 88 332 (83.67) 309 (93.07) 309 (100.00) 173 (55.96) 136 (44.04)
(An. sinensis x An. kleini)F3 x An. sinensis 386 (211, 175) 74 282 (73.05) 265 (93.97) 265 (100.00) 143 (53.96) 122 (46.04)
(An. sinensis x An. kleini)F4 x An. sinensis 413 (240, 173) 79 319 (77.24) 284 (89.02) 259 (91.20) 137 (52.90) 122 (47.10)
(An. sinensis x An. kleini)F5 x An. sinensis 412 (232, 180) 69 282 (68.45) 282 (100.00) 254 (90.07) 139 (54.72) 115 (45.28)
(An. sinensis x An. kleini)F6 x An. sinensis 409 (226, 183) 86 318 (77.75) 308 (96.86) 295 (95.78) 171 (57.97) 124 (42.03)
(An. sinensis x An. kleini)F7 x An. sinensis 365 (147, 218) 87 307 (84.11) 301 (98.05) 295 (98.01) 157 (53.22) 138 (46.78)
(An. sinensis x An. kleini)F8 x An. sinensis 355 (154, 201) 84 284 (80.00) 281 (98.94) 267 (95.02) 139 (52.06) 128 (47.94)
(An. sinensis x An. kleini)F9 x An. sinensis 315 (190, 125) 89 271 (86.03) 271 (100.00) 271 (100.00) 136 (50.18) 135 (49.82)
(An. sinensis x An. kleini)F10 x An. sinensis 336 (162, 174) 94 291 (86.61) 266 (91.40) 257 (96.62) 126 (49.03) 131 (50.97)
(An. kleini x An. sinensis)F1 x An. kleini 497 (239, 258) 74 343 (69.01) 343 (100.00) 343 (100.00) 182 (53.06) 161 (46.94)
(An. kleini x An. sinensis)F2 x An. kleini 427 (226, 201) 77 305 (71.43) 268 (87.87) 268 (100.00) 147 (54.85) 121 (45.15)
(An. kleini x An. sinensis)F3 x An. kleini 421 (209, 212) 70 286 (67.93) 257 (89.86) 257 (100.00) 126 (49.03) 131 (50.97)
(An. kleini x An. sinensis)F4 x An. kleini 458 (211, 247) 79 328 (66.00) 302 (92.07) 287 (95.03) 155 (54.01) 132 (45.99)
F11 hybrid crosses        
[(An. sinensis x An. kleini)F11 x An. sinensis] 329 (171, 158) 96 313 (95.14) 285 (91.05) 242 (84.91) 117 (48.35) 125 (51.65)
x [(An. sinensis x An. kleini)F11 x An. sinensis]        
  1. * Two selective egg-batches of inseminated females from each cross; Dissection from 100 eggs; n = number.
  2. ††Sterile male hybrids with atrophy testes and accessory glands.