Table 1 The four pairs of specific primers used to amplify sodium channel gene mutations detected in Cx. p. quinquefasciatus
Primers | 5′ → 3′Sequence | length (bp) |
|---|---|---|
Cx-M1 | GATGATCATGCCATCCACGC | 548 |
CTTCCTCACATTGGCCAGCA | ||
Cx-M2 | CGACAGTCGAATCTACCGAGGTG | 995 |
CTTCTCCTTGTTGTTGTCGTCG | ||
Cx-M3 | GAGCTGTTCATCACGCTCTG | 689 |
GCCGACAAACTCGAGGAACC | ||
Cx-M4 | GCCGGATAACGACAAGGGTT | 661 |
GGGCTCGTTATCACAGGCAT |