Table 1 PCR primer pairs used in this study
Gene target | Primer name | Primer sequence 5’→ 3’ | Amplified fragment (bp) | Annealing temperature (ºC) | Reference |
|---|---|---|---|---|---|
groESL heat shock operon of Anaplasma spp. (nested) | HS1a | AITGGGCTGGTAITGAAAT | 1350 | 48 | [9] |
HS6a | CCICCIGGIACIAIACCTTC | ||||
HS43 | AT(A/T)GC(A/T)AA(G/A)GAAGCATAGTC | 1297 | 55 | ||
HSVR | CTCAACAGCAGCTCTAGTAGC | ||||
16S rRNA (nested) | ge3a | CACATGCAAGTCGAACGGATTATTC | 932 | 55 | [10] |
ge10r | TTCCGTTAAGAAGGAT CTAATCTCC | ||||
ge9f | AACGGATTATTCTTTATAGCTTGCT | 546 | 55 | ||
ge2 | GGCAGTATTAAAAGCAGCTCCAGG | ||||
16S rRNA EHR | EHR 16SD | GGTACCYACAGAAGAAGTCC | 345 | 55 | [11] |
EHR 16SR | TAGCACTCATCGTTTACAGC |