Vector competence of the tick Ixodes sinensis (Acari: Ixodidae) for Rickettsia monacensis
© Ye et al.; licensee BioMed Central Ltd. 2014
Received: 11 June 2014
Accepted: 30 October 2014
Published: 19 November 2014
Cases of Mediterranean Spotted Fever like rickettsioses, caused by Rickettsia monacensis, have become more common in the last 10 years. In China, natural infection of R. monacensis in various tick species has been confirmed but the vector(s) of R. monacensis have not been recorded.
The prevalence of R. monacensis in >1500 Ixodidae ticks from central and southern China was determined using centrifugation-shell vial culture and polymerase chain reaction techniques. The predominant species, Ixodes sinensis, harbored a natural infection of R. monacensis and was assumed to be a vector candidate of R. monacensis. Experimental transmissions were initialized by infecting Rickettsia-free tick colonies with R. monacensis using capillary tube feeding (CTF) or immersion techniques. Transstadial and transovarial transmissions, and transmission from ticks to mice, were conducted under laboratory conditions.
R. monacensis was isolated and identified from hemolymph of Ixodes sinensis using molecular techniques. Transovarial transmission of R. monacensis from infected ♀I. sinensis to offspring was documented and infected offspring successfully passed Rickettsia to mice. Transstadial transmission rates were 58% in larva to nymph and 56% in nymph to adult stages. Infected nymphs and adults were also able to infect mice.
I. sinensis is a competence vector for R. monacensis as demonstrated by natural infection and transmission studies.
Rickettsiae are obligate intracellular, gram-negative, alpha-proteobacteria usually transmitted by arthropod vectors.They cause various human diseases including emerging spotted fever rickettsiosis . Since the initial study of the spotted fever group (SFG) rickettsia by Ricketts (1906) more than 27 described species and uncharacterized strains have been associated with spotted fever rickettsiosis ,. R. monacensis was first isolated and characterized in 2002 from Ixodes ricinus ticks collected in Munich, Germany . Five years later, R. monacensis was identified from Mediterranean Spotted Fever (MSF)-like patients in Spain . General discomfort, headache, and joint pain, a nonpruritic, disseminated maculopapular rash or an erythematous rash with no inoculation escharare typical symptoms. Since 2007, MSF-like cases have been documented in Italy , Croatia  and the Republic of Korean (EU883092, FJ009429). To determine possible arthropod vectors, vertebrate reservoirs, and geographic ranges, epidemiological surveys for R. monacensis have been performed in several European countries -. The prevalence of R. monacensis in I. ricinus ranged from 4% (Spain), 8.6% (Germany), and 12.2% (Slovakia) to 52.9% (Bulgaria) ,. R. monacensis was also found in Ixodes persulcatus from mainland China , Ixodes nipponensis and Haemaphysalis longicornis from the Republic of Korea, and Haemaphysalis punctata from Italy, suggesting that many tick species are involved in the zoonotic cycles and wide geographic range of R. monacensis. However, the presence of R. monacensis in these ticks does not prove that they are competent vectors of R. monacensis. Transmission data provides better evidence for the potential of Ixodid ticks to serve as vectors for R. monacensis.
Tick-borne rickettsial diseases are a significant problem in China. Over the last 5 years, tick populations have generally increased and this has led to an increase in human tick bites . Two phenomena are striking. First, thousands of hospitalized patients have unexplained febrile illnesses coinciding with the period of greatest tick activity. Data on clinical symptoms, history of exposure to ticks, and presumptive therapy strongly suggests that some of the patients are infected by SFG rickettsial pathogens . Second, the range of human cases appears to be expanding southward. Many human cases with typical spotted fever symptoms have been found in central and southern China. While some common factors may be at play, the mechanisms behind infection and range expansion have not been fully clarified. Infections may be influenced by climate changes, potential vector ticks, host population dynamics, and human behaviour changes. Due to current diagnostic techniques that depend on clinical symptoms and serological and/or commercially available genus-specific PCR assays, detailed information about the pathogens and the vector ticks is scarce.
Knowledge of tick borne rickettsiosis ecology is essential to understand the potential threat of emerging Rickettsia spp. and vector ticks in central and southern China.To address this issue, we surveyed for Rickettsia spp. in Chinese Ixodid ticks. Our focus was on species which frequently bite tourists and residents and are prevalent throughout pastoral and forest areas from Henan, Hubei, Anhui, Shandong, Jiangsu and Zhejiang provinces. The survey was conducted during 2009-2013. R. monacensis was successfully isolated and identified from I. sinensis in Guangshan county, Henan province. I. sinensis is closely related to the well-known vector I. ricinus inmorphology, phylogeny and blood feeding behavior. I. sinensis was therefore assumed to be a probable vector of R. monacensis and its capability for transtadial and transovarial transmission was determined.
Pathogen free, 14-day-old, male C3H mice were provided by the Animal Care Laboratory of the Institute of Zoology, Chinese Academy of Science and served as hosts for both I. sinensis and R. monacensis. C3H mice were maintained in accordance with the Institutional Animal Care and Use Committee of Beijing Institute of Microbiology and Epidemiology.
Cultivation R. monacensis from ticks
Hemolymph from ticks was cultured in human embryonic lung (HEL) fibroblasts with the centrifugation-shell vial technique using 12-mm round cover slips seeded with 1 ml of medium containing 50,000 cells and incubated in a 5% CO2 incubator at 37°C for 3 days to obtain a confluent monolayer . Cultures were monitored for 4 weeks, and bacterial growth was assessed every 7 days on cover slips directly inside the shell vial using Gimenez and immunofluorescence staining methods. For positive cultures, the Rickettsia isolate was identified using PCR and sequencing as described below.
Infection I. sinensis with CTF and immersion methods
Rickettsa-free I. sinensis were fixed on slides individually using double-sided adhesive tape. A drawn-our capillary tube filled with 10 μL of medium containing 500 cells infected with R. monacensis was placed over hypostomes of tick and the tick was allowed to feed at 34°C as described for artificial infection by Borrelia burgdorferi. Pipettes were replaced every 2–3 h for 6 h and then ticks were detached from the double-sided tape and returned to colony maintenance conditions. A similar procedure was performed to infect the Rickettsia free colony of nymphal I. sinensis. To infect larvae by immersion, 50 μL of a medium containing 2,500 cells infected with R. monacensis was cracked with an ultrasonic cell disruptor (800 watt, 2 h) prior to the immersion procedures. Ticks were placed in this medium and vortexed at medium speed and incubated at 34°C for 30 min. To avoid larval flotation the centrifugation was pulsed. Centrifuged larvae were surface disinfected by immersion in a 0.1% bleach solution for 2 min, washed in distilled H2O, and returned to colony maintenance conditions .
Transovaries Transmission, TOT
After infection by CTF, every 4 I. sinensis females were allowed to feed on one naïveC3H mouse along with 4 males. To avoid grooming, each mouse was restrained with a collar. The parasitized mice were reared individually in a cage over water pans, where well fed females were recovered and returned to colony maintenance conditions after dropping from their hosts. The engorged females were maintained individually until egg laying. From each maternal individual, we sampled 300 F1 eggs and allocated them randomly into 3 pools. The rest of the eggs were kept in colony maintenance conditions to hatch. After hatching, 300 F1 larvae were sampled as before and allocated to 3 pools. The filial eggs and resultant larvae pools were submitted to be screened for R. monacensis infection with the PCR and sequencing method described below.
Transstadial Transmission, TS
After infection by CTF or immersion methods, 10 nymphs or 50 larvae of I. sinensis were allowed to feed on one C3H mouse as described above. A total of 15 C3H mice were used as hosts for nymphs and 6 mice were used for larvae. After detachment, the engorged nymphs and larvae were harvested. A total of 30 engorged nymph or 30 engorged larvae were sampled and the rest were maintained individually prior to molting into adult or nymphs. Then, the subsequent 25 males and 25 females or 50 nymphs were also sampled and the rest were used in the following experiment to study transmission from tick to host reservoir. The sampled ticks were tested for R. monacensis infection with PCR and sequencing methods as described below.
Transmission from tick to host reservoir
The transmission competence of tick to naïve mice was tested as follows. Females, nymphs and larvae of I. sinensis obtained from TS and TOT experiments were also allowed to feed on naïve C3H mice respectively as described above. At 5 days following tick detachment, blood was collected by tail vein from each mouse to evaluate for R. monacensis infection using PCR and sequencing methods.
PCR detection for Rickettsia monacensis in cells, tick and mice and sequencing
The QIAamp DNA mini Kits (QIAGEN, Hilden, Germany) were utilized to prepare DNA templates from the cells, ticks and mice samples according to the manufacturer's protocol. All PCR assays used Taq polymerase (Promega) in 50 μL reactions with the manufacturer's suggested buffer and nucleotide concentrations. Presence of rickettsial DNA in tick and mice blood extracts was detected with specific primers as follows: Primer GltA.877p and GltA1258n , which amplify a 382 bp part of glt A gene; Primer Rr17.61 and Rr17.492 , which amplify a 438 bp fragmentof17kD protein gene; Primer Rr70p and Rr602nfor the 530 bp fragment of the omp A gene; RrompBf and RrompBr for a 515 bp fragment of omp A . The PCR were performed as described previously  with distilled water instead of DNA template used as a negative control. All amplicons were cloned into the pGEM-T Easy vector and subjected to bidirectional sequencing (Sangon Biotech, Shanghai, China) with SP6 and T7 promoter primers. The newly obtained sequences were aligned with corresponding sequences retrieved from the GenBank database (http://www.ncbi.nlm.nih.gov) using BioEditv.18.104.22.168. The phylogenetic trees for the genes were constructed applying the Neighbour-Joining (NJ) algorithm implemented in the software package MEGA 5.20.
The study had received the specific approval of the Institutional Animal Care and Use Committee (IACUC) of Beijing institute of Microbiology and Epidemiology. It was informed of the objectives, requirements and procedures of the experiments. Before each feeding process, a single dose of a non-steroidal anti-inflammatory agent (NSAID) Aspirin was orally administrated to mice to alleviate the suffering of the mice, following the guidance of IACUC of Beijing institute of Microbiology and Epidemiology.
R. moncacensis prevalence in ticks and its cultivation from I. sinensis hemolymph
Transmission of R. monacensis byI. sinensis
The potential pathogenicity of R. monacensis to I. sinensis was evaluated by comparison with a control group, fed with the same volume of PBS solutions through capillary tubes. Over 30 d post CTF, ticks died at approximately the same rate with no significant differences between groups. At 5 d after tick detachment from mice, all hemolymph samples collected from 20 engorged females of I. sinensis and blood samples from the infested mice were infected with R. monacensis, indicating successful infection by CTF. The replete females were maintained individually until egg production at approximately 21 d. All 15 egg pools from 5 females yielded positive results as did the 15 larval ones, suggesting a 100% TOT infection rate.
Results for transmission experiments
Subsequent stage or sex
From adult to larva
From larva to nymph
From nymph to adult
Mediterranean Spotted like Fever and its pathogen R. monacensis has been well characterized in previous studies and natural infection of R. monacensis in many tick species has also been confirmed in many countries. However, the details of the maintenance and transmission of R. monacensis in ticks remain incomplete. In Eurasia, I. ricinus has been regarded as the vector in many MSF like cases. Due to its close morphological and phylogenic relationships with I. ricinus, in America I. scapularis, was considered a vector candidate in assessing the possible transmission mechanism by Baldridge et al.. Using green fluorescent protein (GFP) expressing R. monacensis Rmona658, they demonstrated the transmission of R. monacensis in I. scapularis from larvae to nymphs and nymphs to adults. However, TOT and horizontal transmission failed. Rmona658 did not establish in small mammal hosts by the feeding of either I. scapularis nymphs or adults. Thus no other tick species except I. ricinus has been demonstrated to be a vector of R. monacensis until the present report.
Our results estimate the natural infection of I. sinensis with R. monacensis in Chinese locations, suggesting that MSF like rickettsiosis occurs in both central and southern China. The potential threat of R. monacensis should be considered in differential diagnosis in spotted fever patients. Considering natural infection and experimental transmission evidence from TS, TOT and horizontal transmission protocols, I. sinensis appears to be a competent vector. I. sinensis has frequently been recorded feeding on residents and tourists in central and southern China , so the potential health risks of I. sinensis and R. monacensis should be recognized by public health authorities. The vector competence of I. sinensis helps to explain the trend of southward expanding range of MSF-like rickettsiosis in China. The valid geographic range of I. sinensis is not limited to the areas sampled in this report ,, therefore, MSF-like rickettsiosis might occupy larger geographical areas in China.
In our survey, R. monacensis was only found in I. sinensis; but it has previously been recorded in I. persulcatus. No I. persulcatus were collected in the present study and this species mainly occurs in the north and northeast China . The southern range limit of I. persulcatus is in the Qiling-Taihang-Yanshan Mountains ,. Our survey sites did not include this range and we collected neither R. sibrica nor R. heilongjiangensis, which often co-occurs with I. persulcatus. Thus, we assume misidentification of ticks might have occurred in the report of Li et al. because of the morphological similarity of the two species (they belong to the same complex) and despite the fact that temporal and spatial factors and even the low prevalence might contribute to the differences.
I. sinensis belongs to the I. ricinus species complex which is important in the animal to human transmissionof tick borne pathogens such as, Borrelia burgdorferi, Babesia protozoans, Anaplasma phagocytophilum. I. sinensis is the principal vector of the Lyme disease agent Borrelia burgdorferi and related Borrelia species in southern China . Because the transmission cycle of R. monacensis by I. sinensis appears similar to that of Borrelia spp, co- infection of humans by R. monacensis and other tick borne pathogens might occur in some regions. The co-infection prevalence in human populations and the related public health risks will require further investigation.
As demonstrated by natural infection and transmission studies, I. sinensis is a competence vector for R. monacensis, the agent for Mediterranean Spotted Fever like rickettsiosis.
We are grateful to Prof. G Xu for reviewing the manuscript. We thank LetPub for its linguistic assistance during the preparation of this manuscript. This study was supported by the National Science Foundation of China (30400364, 81271878), Special Fund of the Ministry of Health of P. R. China (Grant no. 201202019) and National Critical Project for Science and Technology on Infectious Disease of P. R. China (Grant No.2012ZX10004219) for funding the research.
- MerhejVand Raoult D: Rickettsial evolution in the light of comparative genomics. Rev Camb Philos Soc. 2011, 86: 379-405. 10.1111/j.1469-185X.2010.00151.x.View ArticleGoogle Scholar
- Raoult D, Parola P: Rickettsial Diseases. 2007, Informa Healthcare USA, Inc, New YorkView ArticleGoogle Scholar
- Ricketts HT: The transmission of Rocky Mountain spotted fever by the bite of the wood tick (Dermacentor occidentalis). J Am Med Assoc. 1906, 47: 358-10.1001/jama.1906.25210050042002j.View ArticleGoogle Scholar
- Simser JA, Palmer AT, Fingerle V, Wilske B, Kurtti TJ, Munderloh UG:Rickettsia monacensis sp. nov., a Spotted Fever Group Rickettsia, from Ticks (Ixodes ricinus) Collected in a European City Park. Appl Environ Microbiol. 2002, 68: 559-4566. 10.1128/AEM.68.9.4559-4566.2002.Google Scholar
- Jado I, José AO, Mikel A, Horacio G, Raquel E, Valvanera I, Joseba P, Aranzazu P, María JL, Cristina GA, Isabel RM, Pedro A:Rickettsia monacensis and Human Disease, Spain. Emerg Infect Dis. 2007, 13: 1405-1407. 10.3201/eid1309.060186.PubMed CentralView ArticlePubMedGoogle Scholar
- Madeddu G, Fabiola M, Antonello C, Alessandra C, Sergio B, Ivana M, Maria LF, Giovanni R, Maria SM: Rickettsia monacensis as cause of Mediterranean spotted fever–like illness. Italy Emerg Infect Dis 2012, 18:702–704.Google Scholar
- Tijsse-Klasen E, Hein S, Nenad P: Co-infection of Borrelia burgdorferi sensu lato and Rickettsia species in ticks and in an erythema migrant's patient. Parasit Vectors. 2013, 6: 347-10.1186/1756-3305-6-347.PubMed CentralView ArticlePubMedGoogle Scholar
- Márquez FJ: Spotted fever group Rickettsia in ticks from southeastern Spain natural parks. Exp Appl Acarol. 2008, 45: 185-194. 10.1007/s10493-008-9181-7.View ArticlePubMedGoogle Scholar
- Beninati L, Sacchi L, Genchi C, Bandi C: Emerging rickettsioses. Parassitologia. 2004, 46: 123-126. Article in ItalianPubMedGoogle Scholar
- Dobler G, Essbauer S, Wolfel R: Isolation and preliminary characterization of `Rickettsia monacensis' in south-eastern Germany. Clin Microbiol Infect Dis. 2009, 15: 263-264. 10.1111/j.1469-0691.2008.02227.x.View ArticleGoogle Scholar
- Rymaszewska A, Piotrowski M: Use of DNA sequences for Rickettsia identification in Ixodes ricinus ticks: the first detection of Rickettsia monacensis in Poland. Microb Infect. 2013, 15: 140-146. 10.1016/j.micinf.2012.11.005.View ArticleGoogle Scholar
- Christova I, Pol J, Van D, Yazar S, Velo E, Schouls L: Identification of Borrelia burgdorferi sensu lato, Anaplasma and Ehrlichia species, and spotted fever group Rickettsiae in ticks from Southeastern Europe. Eur J Clin Microbiol Infect Dis. 2003, 22: 535-542. 10.1007/s10096-003-0988-1.View ArticlePubMedGoogle Scholar
- Sekeyova Z, Fournier PE, Rehacek J, Raoult D: Characterization of a new spotted fever group rickettsia detected in Ixodes ricinus (Acari: Ixodidae) collected in Slovakia. J Med Entomol. 2000, 37: 707-713. 10.1603/0022-2585-37.5.707.View ArticlePubMedGoogle Scholar
- Li W, Liu L, Jiang X, GuoX GM, RaoultD PP: Molecular identification of spotted fever group Rickettsiae in ticks collected in central China. Clin Microbiol Infect. 2009, 15: 279-280. 10.1111/j.1469-0691.2008.02235.x.View ArticlePubMedGoogle Scholar
- Shin SH, Seo HJ, Choi YJ, Choi MK, Kim HC, Klein TA, Chong ST, Richards AL, Park KH, Jang WJ: Detection of Rickettsia monacensis from Ixodes nipponensis collected from rodents in Gyeonggi and Gangwon Provinces, Republic of Korea. Exp Appl Acarol. 2013, 61: 337-347. 10.1007/s10493-013-9699-1.View ArticlePubMedGoogle Scholar
- Lee KM, Choi YJ, Shin SH, Choi MK, Song HJ, Kim HC, Klein TA, Richards AL, Park KH, Jang WJ: Spotted fever group rickettsia closely related to Rickettsia monacensis isolated from ticks in South Jeolla province, Korea. Microbiol Immunol. 2013, 57: 487-495. 10.1111/j.1348-0421.2012.00515.x.View ArticlePubMedGoogle Scholar
- Jia N, Zheng YC, Ma L, Huo QB, Ni XB, Jiang BG, Chu YL, Jiang RR, Jiang JF, Cao WC: Human Infections with Rickettsia raoultii, China. Emerg Infect Dis. 2014, 20: 866-868. 10.3201/eid2005.130995.PubMed CentralView ArticlePubMedGoogle Scholar
- Patton TG, Dietrich G, Brandt K, Dolan MC, Piesman J, Gilmore RD: Saliva, salivary gland, and hemolymph collection from ixodes scapularis ticks. J Vis Exp. 2012, 60: e3894-Google Scholar
- Kurtti TJ, Munderloh UG, Hughes CAN, Engstrom SM, Johnson RC: Resistance to tick-borne spirochete challenge induced by Borrelia burgdorferi strains that differ in expression of outer surface proteins. Infect Immun. 1996, 64: 4148-4153.PubMed CentralPubMedGoogle Scholar
- da Quesa M, Sanfeliu I, Cardeáosa N, Segura F: Ten years' experience of isolation of Rickettsia spp. from blood samples using the shell-vial cell culture assay. Ann N Y Acad Sci. 2006, 1078: 578-581. 10.1196/annals.1374.115.View ArticleGoogle Scholar
- Korshus JB, Munderloh UG, Bey RG, Kurtti TJ: Experimental infection of dogs with Borrelia burgdorferi sensu stricto using Ixodes scapularis ticks artificially infected by capillary feeding. Med Microbiol Immunol. 2004, 193: 27-34. 10.1007/s00430-003-0178-x.View ArticlePubMedGoogle Scholar
- Mitzel DN, Wolfinbarger JB, Daniel Long R, Max M, Best SM, Bloom ME: Tick-borne flavivirus infection in Ixodes scapularis larvae: development of a novel method for synchronous viral infection of ticks. Virology. 2007, 365: 410-418. 10.1016/j.virol.2007.03.057.PubMed CentralView ArticlePubMedGoogle Scholar
- Regnery RL, Spruill CL, Plikaytis BD: Genotypic identification of rickettsiae and estimation of intraspecies sequence divergence for portions of two rickettsial genes. J Bacteriol. 1991, 173: 1576-1589.PubMed CentralPubMedGoogle Scholar
- Noda H, Munderlonh UG, Kurtti IJ: Endosymbionts of ticks and their relationship to Wolbachia spp and tick-borne pathogens of human and animals. Appl Environ Microbiol. 1997, 63: 3926-3932.PubMed CentralPubMedGoogle Scholar
- Fournier PE, Dumler JS, Greub G, Zhang JZ, Wu YM, Raoult D: Gene Sequence-Based Criteria for Identification of New Rickettsia Isolates and Description of Rickettsia heilongjiangensis sp. nov. J Clin Microbiol. 2003, 41: 5456-5465. 10.1128/JCM.41.12.5456-5465.2003.PubMed CentralView ArticlePubMedGoogle Scholar
- Baldridge GD, Kurtti TJ, Burkhardt N, Baldridge AS, Nelson CM, Oliva AS, Munderloh UG: Infection of Ixodes scapularis ticks with Rickettsia monacensis expressing green fluorescent protein: a model system. J Invertebr Pathol. 2007, 94: 163-174. 10.1016/j.jip.2006.10.003.PubMed CentralView ArticlePubMedGoogle Scholar
- Teng KF, Jiang ZJ: Economic insect faunaof China, Acari, Ixodidae. Ixodidae. Edited by: Teng IKF, Jiang ZJ. 1991, Science Press, Beijing, 81-234.Google Scholar
- Sun Y, Xu RM, Cao WC:Ixodes sinensis: competence as a vector to transmit the Lyme disease spirochete Borrelia garinii. Vect Born Zoon Dis. 2003, 3: 39-44. 10.1089/153036603765627442.View ArticleGoogle Scholar
- Guglielmone AA, Robbins RG, Apanaskevich DA, Petney TN, Estrada-Peña A, Horak IG: The Hard Ticks of the World (Acari: Ixodida: Ixodidae). 2014, Springer Science, Dordrecht, NetherlandsView ArticleGoogle Scholar
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