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Table 1 Primer sequences used for cloning and qPCR in this study

From: Purification and characterization of a novel defensin from the salivary glands of the black fly, Simulium bannaense

Primer

Sequence (5′ → 3′)

Application

3′ PCR primer

CGGGGTACGATGAGACACCA

3′ end screening

SibaDef -F1

TIYTIWSIATHWSNACNCC*

3′ end screening

SibaDef -R1

TCGTACATCAGTCAGATCCACCG

5′ end screening

5′ PCR primer

AAGCAGTGGTATCAACGCAGAGT

5′ end screening

SibaDef - F2

AGAAGAGCAACCTGCGACCTG

qPCR

SibaDef - R2

AGTCAGATCCACCGCCCGAAT

qPCR

Actin-F

TGTTGTCACTGTACGCCTCCG

qPCR

Actin-R

TGATGTCGCGAACGATTTCCC

qPCR

  1. *Where Y stands for C or T, W stands for A or T, S stands for C or G , H stands for A, C or T, N stands for A, C, G or T, and I stands for hypoxanthine.
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Parasites & Vectors

ISSN: 1756-3305

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