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Ectoparasites of murids in peninsular Malaysia and their associated diseases


A considerable number of rat-borne ectoparasite studies have been conducted since the early 1930s in the Malayan Peninsula (now known as peninsular Malaysia). The majority of studies were field surveys and collections of specimens across the region, and were conducted primarily to catalogue the ectoparasite host distribution and discover novel species. This has generated a signification amount of information, particularly on the diversity and host distribution; other aspects such as morphology, host distribution and medical significance have also been investigated. Amongst the four main groups (mites, fleas, ticks, lice), rat-borne mites have received the most attention with a particular emphasis on chiggers, due to their medical importance. More recent studies have examined the distribution of ectoparasites in rats from different habitat type simplicating a high prevalence of zoonotic species infesting rat populations. Despite being capable of transmitting dangerous pathogens to human, the health risks of rat-borne ectoparasites appear to be small with no serious outbreaks of diseases recorded. Although an extensive number of works have been published, there remain gaps in knowledge that need to be addressed, such as, the distribution of under studied ectoparasite groups (listrophorids and myobiids), determining factors influencing infestation, and understanding changes to the population distribution over time.


Rats have successfully exploited a wide variety of habitats and environments throughout the world. In many places, commensal rodents have adapted to living in close association with humans, using human agriculture products and waste as their food resources and buildings as their homes. “You are never more than 6 ft away from a rat” may be an urban myth, but demonstrates the intimate relationship between rats and human urban dwellers [1]. Rats, particularly species that live close to humans, play an important role in public health and the economy. Zoonotic diseases transmitted directly by rats through bites, urine and faeces include Leptospirosis [2], which affects 7-10 million people per year globally. However, perhaps more important is the indirect transmission of diseases that takes place through rat-borne ectoparasites such as the flea, Xenopsylla cheopis; the vector of causative agents, Yersinia pestis, responsible for the plague, and Rickettsia typhi, responsible for typhus [3].

Historically, collections and surveys of ectoparasites across the region now known as peninsular Malaysia, have been conducted to record and catalogue the various local rat-borne ectoparasite species [4-8], to determine their geographical distribution and host specificity [9-11], and in particular collect data regarding the incidence of medically significant species [12,13]. Since the 1940s, a significant amount of data has been accumulated on diversity and host distributions of rat-borne ectoparasites but surveys have, for pragmatic reasons, mostly focused on restricted taxonomics groups (e.g. ticks [9]; fleas [14]) or limited localities or habitats [4,6-9,12,15-22]. In surveys, rat-borne ectoparasites are generally categorized into four major groups, i.e. mites, fleas, ticks, and lice. Rat-borne mites, a highly diverse and specialized group, are further divided into chiggers, mesostigmatid mites, listrophorids and myobiids. Other published works related to the ectoparasite surveys include (i) descriptions of new species [23-25] and other taxonomic analyses [26,27], (ii) studies of ectoparasite morphology [27-29] and habitat [30,31], (iii) seasonal variations in diversity [17,32], and (iv) isolation of medically significant pathogens [33-35]. Other aspects such as lifecycle [36,37], and behaviour [38,39] have also been investigated but to a lesser degree.

Comprehensive surveys or reviews regarding rat-borne ectoparasites in peninsular Malaysia have seen commendable consistency in the frequency of published studies on the local rat-borne ectoparasite fauna since the early 1930’s [18,40-42] with the notable works in recent years [21,22,43,44]. Therefore, the objective of this review is to compile and correlate the available information on ectoparasite-host interactions and ectoparasite behaviour. This will lead to better perspectives on the epidemiology of related diseases as well as identify gaps in knowledge which may act to stimulate future studies.




Following Gater’s study in 1932 [42], scientific interest in rat-borne ectoparasites was ignited by a concerted effort from the late 1940’s to early 1950’s [43-45] to investigate the epidemiology of scrub typhus in the Asia-Pacific Region. It was quickly established that chiggers (Arachnida: Trombiculidae), particularly Leptotrombidium deliensis, are the primary vectors for the disease. The incidences of chiggers were found to be closely associated with the presence of wild rats prompting subsequent studies to examine the ecological distribution of both chiggers and their hosts [16,37,45-47].

It soon became clear that chiggers from peninsular Malaysia are a uniquely ubiquitous and diverse group being reported in virtually every local general rat-borne ectoparasite survey [6-8]. Walchiella oudemansi, Leptotrombidium deliense and Gahrliepia fletcheri are the more commonly observed species [37]. Chiggers then began to generate interest independently of their association with thypus and there was a sudden increase in reports on the local, often novel, species [48-50]. A substantial portion of the reports in the Malaysian Parasite series by the Institute for Medical Research have focussed on chiggers. The overwhelming collection of data concerning the morphology, ecology, hosts, behavior, population control and medical significance of local chiggers was incorporated into an important early review paper [15,37,51]. The summary of the presence of chiggers and its hosts are as described in Table 1.

Table 1 Chiggers (Acari, Prostigmata, Trombiculidae) in peninsular Malaysia

Mesostigmatid and other mites

Among the rat-borne ectoparasites groups; rat mites (Arachnida) have received the most attention. Rat mites are a highly diverse and specialized group that includes chiggers (see above), mesostigmatids, listrophorids and myobiids. Major general works include Domrow and Nadchatram [10], Domrow [25] and Ismail et al. [11]. Lately, attention has begun to shift from chiggers toward the mesostigmatid mites [11,13], another similarly widespread and species-rich group. These recent studies have accumulated information on host distribution, diversity and morphological characteristics. Laelaps echidninus, Laelaps nutalli, Laelaps sedlaceki L. turkestanicus L. sanguisugus and Longolaelaps whartoni are some of the more common rat-borne mesostigmatids in peninsular Malaysia [21,44]. Other less common species include Haemolaelaps gallinarii [52]; Haemolealaps argentiventer [53]; Haemogamasus liberiensus and Tricholealaps vitzthumi [54,55].

In contrast, information on the distribution and prevelance of the other two rat mite groups, the listrophorids (Table 2) and myobiids (Table 3) is still preliminary with only eighteen and ten species having been recorded respectively. This is possibly due to the fact that these ectoparasites have shown little or no medical and ecological significance. Listrophorids infestations among the local wild rats have only been reported occasionally [4,5]. Even less has been reported about diversity and distribution of rat myobiids [4]. Presently, two publications by Fain and collagues [56,57] remain the definitive references on the local rat-borne listrophorids and myobiids.

Table 2 Listrophoroides Hirst, 1923 (Acari, Astigmata, Atopomelidae) of rats in peninsular Malaysia
Table 3 Myobiidae (Prostigmata) from rats in peninsular Malaysia


Documentation of rat-borne fleas (Insecta: Siphonaptera) and ticks (Arachnida: Ixodida) also began during the 1940s and 1950s [58], and continued intermittently over the following years [13,18,21,41]. There has only been two long-term surveillance studies on the incidence of fleas among local small mammals [14,59]. Both studies recognized Xenopsylla cheopis as the most prevalent rat-borne flea in the region. Zahedi et al. [60] showed that urban rat populations had a higher flea index compared to semi-urban rat populations. They also reported 6% of the rats trapped in the city harboured the cat flea, Ctenocephalides felis [60]. Other species include Stivalius ahalae [6], Rothschildiana smiti [23] and interestingly, Xenopsyllaastia, a common cosmopolitan species, has been observed only once [3]. Stivalius jacobsoni on Niniventer rapit, Stivalius klossi on Niniventer cremoriventer and Paraceras sp. on Maxomys edwardsi have been reported by Traub [58].


The first major study on rat-borne ticks from peninsular Malaysia was conducted by Kohls [9], who compiled a host-parasite checklist and a comprehensive taxonomic key. The study provided the basis for further investigations into the host distribution of rat-borne ticks in the region [61,62]. Extensive collections have revealed wild terrestrial rats to be a common, perhaps preferred, host for immature ticks (e.g. Amblyomma spp., Haemphysalis spp. and Dermacentor spp.), while Ixodes granulatus was the only adult tick found on rats [9-12,16,17,63]. Interestingly, Nadchatram et al., 1966 recorded Amblyomma helvoluma species usually found on reptiles on a rat, Leopoldamys sabanus [16]. However, difficulties identifying immature rat-borne ticks beyond the genus level have hindered efforts to associate tick infestations with the behavioral and ecological data on their hosts.


Studies concerning rat-borne lice have focused on morphology for taxonomic identification and phylogenetics [13,28,64]. Presently, Hoplopleura pacifica, H. dissicula, H. pectinata and Polyplax spinulosa have been recorded from peninsular Malaysia [21,65]. Lice have only been included occasionally in the general rat-borne ectoparasites surveys [6,7,18,21,22,41].

Breaking the taxonomic boundaries

Early rat-borne ectoparasite surveys showed a propensity to limit their focus on specific groups, as evident in the review by Audy [15]. As a result, the overall rat-borne ectoparasite community has been largely neglected and the intercommunity population dynamics are poorly understood. Recently there have been attempts to redress the situation [4,11,41].

Perhaps for pragmatic reasons, past researchers seemed content in focusing on cataloguing the ectoparasites’ host distribution and describing novel species. The majority of reports are strictly short-term field observations and prevalence studies, and though several extensive, noteworthy works were produced [9,28,48], the seemingly perfunctory approach has restricted further insights into the factors influencing the development, behaviour and transmission of rat-borne ectoparasite species. Additionally, besides for chiggers and ticks, there had been virtually no experimental investigations conducted on local rat-borne ectoparasites [36,38,66]. This may be due to the fact rat-borne ectoparasites do not represent immediate zoonotic risks.

A study by Ho and Krishnasamy [12] warrants a special mention as a survey in which both the ectoparasite and endoparasite of small mammals from Taman Negara was recorded concurrently. Although the study was little more than a host-parasite list, it raises the issue of the probable links between concurrent rat-ecto and endoparasite infections, in which the ectoparasites may serve as intermediate hosts for certain helminthes.

Identification and morphology

Identification of rat-borne ectoparasites had thus far been entirely based on the morphological characteristics of the specimens using available keys and published reference manuals [9,28,67]. Morphological studies therefore placed an emphasis on in-depth and reliable descriptions of the local species and are often accompanied with detailed graphics [25,28]. Perhaps curiously, the most detailed morphological descriptions of the rat-borne ectoparasite fauna of peninsular Malaysia were those of rat-borne lice and ticks while the morphology of rat myobiids and listrophorids from peninsular Malaysia have yet to be systematically and thoroughly described.

Distribution and prevalence patterns

Rat-borne ectoparasites have been reported from various habitats throughout peninsular Malaysia, including primary lowland forest [11,19], secondary forest [5], montane forests [29,68], cities [14,18], cultivated fields [69,70] and coastlines [31]. The majority of collections have been from primary rainforests but several studies have also conducted concurrent investigations of more than one habitat [10,28,40].

These surveys have enabled comparions of the diversity of rat-borne ectoparasites from different habitats across peninsular Malaysia and revealed that generally, each ectoparasite species is restricted to a certain habitat [71-73]. For example, rat-borne fleas and lice were commonly recorded in urban areas but were rarely recorded anywhere else [60]. The rat-borne ectoparasite distribution is therefore assumed to be closely linked to the host species’ habitat specialisation. Audy [27] remarked that for chiggers, “habitat-specificity may give an appearance of host-specificity, and occasionally it may not be possible to explain findings confidently by one explanation or the other”. Similar thoughts were also expressed by several authors [32,44,66,74] and while it may seem reasonable to assume that a degree of host specificity exists, its actual role, as well as the extent of its effects, still needs to be clarified.

The impact of human activities on the disease patterns of rat-borne infections has only been briefly remarked upon [66] and anthropogenic changes to the habitat of rats appear to have unpredictable consequences for the prevalence and distribution of rat-borne ectoparasites [66,70,75,76]. The severity and long-term effects are still largely unclear. Surveys in Taman Negara [12,19,35] and Gombak Forest Reserve [4,11] could provide the basis for long-term studies that would enable future researchers to detect changes in the diversity and intensity of rat ectoparasite infestations that may occur as a result of human impact. Traub and Wisseman [70] hypothesized that rats occupying permanent nests would exhibit higher mite burdens because mites, particularly mesostigmatids, are nest dwellers and that infestations are acquired during the hosts’ time in the nest. The authors further speculated that spiny- furred rats provide a more conducive environment for mites, in contrast to soft-furred and semi-spiny-furred hosts, thereby contributing to the supposed higher mites loads in such hosts. However, no studies have set out to futher test this hypothesis.

There have been no attempts to investigate the effects of the age and sex of hosts on ectoparasite burdens and transmission, but studies have indicated a link between host size and parasite abundance [32].

The effect of seasonal changes and rainfall on ectoparasite burdens have only been briefly explored. It has been hypothesized that dry conditions may have an adverse effect on chigger infestations [32,51]. Lim [17] was unable to conclusively demonstrate the association of rainfall with immature tick infestations, while Singh et al. [14] likewise did not observe any relationship between rainfall and flea indices. The altitudinal distribution of rat-borne ectoparasites has been investigated by Hoogstraal et al. [77] and Nadchatram [68], who found ticks were found in reasonable numbers at the higher altitudes, while chiggers were virtually absent. The actual effects of altitude on the distribution of rat-borne ectoparasites are however not readily apparent and warrant further study.

Vectors of human diseases

Rat ectoparasites have long been known to be capable of transmitting human diseases such as (i) scrub typhus, (ii) murine typhus, (iii) other types of typhus, (iv) the plague, (v) Bartonella and (vi) various viruses. In fact, the potential threat to public health has been the major justification for many studies [35].

  1. (i)

    Scrub typhus, a rickettsial disease transmitted through the bite of an infected chigger, is arguably one of the more well-known diseases associated with the rat-borne ectoparasites in peninsular Malaysia. It is a common cause of fever in rural populations [78,79]. The distribution of scrub typhus has been extensively studied, and is generally associated with cultivated fields and secondary forests, and its epidemiology is linked to human behaviour and occupation [80]. Leptotrombidium deliensis, frequently found infesting wild rats living within or near the vicinity of agriculatural land and secondary forests, is the major vector for Orientia tsutsugamushi, the causative agent for the disease [37,58]. Other chigger species have also been implicated in maintaining and transmitting the disease [35,81,82]. An exhaustive and highly comprehensive review of scrub typhus and various related factors by Traub and Wisseman [51] concluded that chiggers of the subgenus Leptotrombidium associated with rats from the genus Rattus were able to penetrate new areas and habitats and diversify, and as the chiggers adapted to other hosts in the new regions, such mammals became secondarily involved in the ecology of the rickettsiosis.

  2. (ii)

    Murine typhus, also known as urban typhus, is another rat-borne disease from the typhus group. The causative agent is Rickettsia typhi and infection can be transmitted from rats to humans though rat-borne fleas, Xenopsylla cheopis [83]. Murine typhus is still considered uncommon despite indications that the disease is more widespread than previously thought [34,80,84].

  3. (iii)

    There has been indications of a “jungle cycle” scrub typhus in peninsular Malaysia [51,58,85]. Cadigan Jr et al. [86] and Robinson et al. [87] linked the length of residence in forested areas with the infection rate among humans. Though the existence of other scrub typhus strains remains in question, the risks of typhus from primary forests is estimated to be considerably less compared to areas typically associated with the disease [35]. Occurrences of tick typhus or spotted fever group rickettsiae (SFGR), another rickettsioses associated with rat-borne ectoparasites, have also been recorded in peninsular Malaysia [33,80,84]. Marchette [33] implicated Ixodes granulatus and Dermacentor auratus in their transmission.

  4. (iv)

    Rat-borne fleas are also known vectors of Yersinia pestis, the bacterial agent of plague. While there has been no outbreaks in recent years in peninsular Malaysia, plague is endemic in other Southeast Asian countries, including Indonesia [88,89], Thailand [90], Vietnam [91] and Myanmar [92]. The first case of plague in Malaysia occurred in Penang in 1896 and the most recent case was in Perak in 1928 [3].

  5. (v)

    The occasional rat-borne flea (Ctenocephalides felis) and rat-borne tick (Ixodes granulatus) are also often implicated in the natural maintenance of various species of Bartonella. A recent study observed five Bartonella species (Bartonella tribocorum, B. rattimassiliensis, B. coopersplainsensis B. elizabethae and B. queenslandensis) circulating in the rat population in Kuala Lumpur [93].

  6. (vi)

    Rat-bourne ticks are also known hosts of the Langat Virus Langat Virus [94], the forest cycle of Q-fever [33], and the Lanjan Virus [95]. Lanjan virus has been isolated from immature Dermacentor [95,96].

Aside from harbouring and transmitting dangerous pathogens, the ectoparasites themselves are also capable of causing skin irritations for humans. Among the local rat-borne ectoparasites, mesostigmatids i.e. Ornithonyssus bacoti, Laelaps echidninus and Laelaps nuttalli, have been reported as attacking humans, resulting in varying degrees of dermatitis [67,97]. Chiggers are also known to occasionally cause irritations commonly known as scrub itch [12].

Human health concerns associated with chiggers are rural in nature, confined to secondary forest and plantations i.e. areas with low population density. Although the threat of chiggers on human health is fortunately limited, its impact on ecotourism and other daily human activities are often overlooked. Rat-borne fleas appear to pose the highest health risk to humans as their rat hosts and distribution are concentrated within or near high-density human habitations but there has been no serious outbreaks of diseases associated with rat-borne fleas. Likewise the medical significance of rat-borne ticks on public health is presently strictly academic. Despite being shown to be capable of harbouring several dangerous pathogens, there has yet to be any verified reports of tick related infections in peninsular Malaysia. The other ectoparasites groups, the rat-borne lice, myobiids and listrophorids also are presently of no known medical importance [98]. Further investigations should be initiated to update and verify their significance to public health.

To summarize, the collective impact of the local rat-borne ectoparasites on public health is presently low, perhaps even negligible. However, the above studies have shown that these ectoparasites harbour a number of serious pathogens, and could potentially become major health risks in the future. To circumvent this, population dynamics of rat-borne ectoparasites and the epidemiology need to be better understood.

Major gaps in present knowledge of rat-borne cctoparasites of peninsular Malaysia

Quantitative data and statistical analyses

Despite consistent attempts to survey and catalogue the various rat-borne ectoparasite species throughout the region, the collection of quantitative data and statistical analysis of the infestations have been surprisingly inadequate. Though a few surveys did calculate the prevalence of the infestations [4,5,18] and mean intensity values [17,32], measures of variability seem mostly to have been disregarded. With the exception of the work of Ismail et al. [11], diversity and aggregation indices, commonly found in the more recent international parasitological publication, have failed to be incorporated into the peninsular Malaysia investigations. Most surveys conducted have been little more than a host-parasite checklists.

  1. 1.

    Interspecific and intraspecific interactions

    Inter- and intraspecific interactions of different ectoparasite species have never been fully explored. As it is commonly observed that wild terrestrial rats often harbour concurrent ectoparasites infections it would therefore be of great interest to observe how these ectoparasites interact and affect one another within the limited space (e.g. crowding effects, competition, etc.).

  2. 2.

    Natural resistant of hosts

    While briefly mentioned in Boese’s study [99], the pathological aspects of ectoparasite infestation of rats are poorly documented. Virtually no information is available on natural resistance and immunological reactions of the host with ectoparasite infestations.

  3. 3.

    Niche specialization

    There have been observations that certain chiggers species from peninsular Malaysia occupy a specific site within their hosts, such as the nasal cavities [100]. These unique microhabitats have since become an important characteristic for species identification [26,101]. The existence of site specificity is intriguing and reflects the degree of interspecific interactions that may occur in concurrent infestations. Surprisingly however, the phenomenon was not investigated further and it is not known if the species of other ectoparasite groups exhibit a similar behaviour.

  4. 4.

    Morphology and identification

    Most studies carried out by scientists in peninsular Malaysia adopt the conventional method for ectoparasite identification i.e using morphological keys. Using this method only experienced taxonomists are able to distinguish species reliably. The availability of molecular biology as a tool to assist identification has become common place [102], however, there is neither interest nor urgency among researchers to conduct molecular characterization on the local rat-borne ectoparasite species.


Considerable attention has been given towards the ectoparasites of the wild, terrestrial rats in peninsular Malaysia. The majority of rat-borne ectoparasitological studies were field surveys and collections of specimens from across the region although other aspects such as morphology, host distribution and medical significance have also been investigated. Local rat-borne ectoparasite studies often focus on selected groups, particularly chiggers due to their impact on human health, although in recent years attention has slowly shifted towards the overall ectoparasite diversity. Studies on rat-borne ectoparasites have been invaluable in the fields of ecology and public health.

Despite the number of studies that have been published, a number of related issues have to be addressed. Distribution patterns of certain rat-borne ectoparasites still remains unknown. Factors influencing infestations as well as changes to the population distribution over time are also poorly understood. Additionally, the quantitative aspects of ectoparasite infestations have also been largely neglected. It is therefore vital for researchers in the field to redevelop and reprioritize their focus towards these lesser known aspects.


  1. 1.

    Mokoena T. Urban Myths: Are you never more than 6 ft from a rat in a city? The Guardian Online. Available at 2014.

  2. 2.

    Thayaparan S, Robertson I, Fairuz A, Suut L, Abdullah M. Leptospirosis, an emerging zoonotic disease in Malaysia. Malays J Pathol. 2013;35(2):123–32.

    CAS  PubMed  Google Scholar 

  3. 3.

    Anon. Fifty years of Medical Research in Malaya. Plague. Editors: Field, J.W., Green, R. & Byron, F.E. Stud Inst Med Res Fed Malaya. 1950;25:243–52.

    Google Scholar 

  4. 4.

    Shabrina M. Ectoparasites of small mammals trapped at the Ulu Gombak Forest, Selangor Darul Ehsan. J Wildlife Parks. 1990;10:9–17.

    Google Scholar 

  5. 5.

    Shabrina MS, Salleh I. A study on the parasitofauna of rodents and bats at Serting, Negeri Sembilan. J Wildlife Parks. 1995;15:33–42.

    Google Scholar 

  6. 6.

    Mariana A, Zuraidawati Z, Ho TM, Mohd Kulaimi B, Saleh I, Shukor MN, et al. A survey of ectoparasites in Gunung Stong Forest Reserve, Kelantan, Malaysia. Southeast Asian J Trop Med Public Health. 2005;36:25–31.

    Google Scholar 

  7. 7.

    Mariana A, Zuraidawati Z, Ho TM, Kulaimi BM, Saleh I, Shukor MN, et al. Ticks (Ixodidae) and other ectoparasites in Ulu Muda Forest Reserve, Kedah, Malaysia. Southeast Asian J Trop Med Public Health. 2008;39:496–506.

    CAS  PubMed  Google Scholar 

  8. 8.

    Mariana A, Mohd Kulaimi B, Halimaton I, Suhaili ZA, Shahrul-Anuar MS, Nor Zalipah M, et al. Acarine ectoparasites of Panti Forest Reserve in Johore. Asian Pac J Trop Biomed. 2011;1:1–5.

    PubMed Central  CAS  PubMed  Article  Google Scholar 

  9. 9.

    Kohls GM. Malaysian parasites. XVIII. Ticks (Ixodoidea) of Borneo and Malaya. Stud Inst Med Res Fed Malaya. 1957;28:65–94.

    Google Scholar 

  10. 10.

    Domrow R, Nadchatram M. Two field collections of Malayan ticks and mites. Malay Nat J. 1963;17:145–64.

    Google Scholar 

  11. 11.

    Ismail S, Omar B, Sulaiman S, Sulaiman J, Zolkaflee Z. Notes on the ectoparasites of rodents from the Ulu Gombak Forest Reserve, Selangor. Trop Biomed. 2003;20(2):159–63.

    Google Scholar 

  12. 12.

    Ho TM, Krishnasamy M. Ectoparasites acari and endoparasites of small mammals in Taman Negara. J Wildlife Parks. 1990;10:54–61.

    Google Scholar 

  13. 13.

    Chuluun B, Mariana A, Ho T, Mohd Kulaimi B. Research Note A preliminary survey of ectoparasites of small mammals in Kuala Selangor Nature Park. Trop Biomed. 2005;22(2):243–7.

    CAS  PubMed  Google Scholar 

  14. 14.

    Singh KI, Ambu S, Krishnasamy M, Rosli R, Jeffery J. Surveillance of fleas from small mammals from Kuala Lumpur. Trop Biomed. 1995;12:17–21.

    Google Scholar 

  15. 15.

    Audy J. Malaysian Parasites—XXXIV. A review of the literature. Stud Inst Med Res Malaya. 1957;28:427–47.

    Google Scholar 

  16. 16.

    Nadchatram M, Damrow R, Ng CK. Parasitic Acarina of the mammals. Bull Nat Mus Singap. 1966;34:129–40.

    Google Scholar 

  17. 17.

    Lim BL. Host-relationships and seasonal abundance of immature ticks (Haemaphysalis spp. and Dermacentor spp.) in primary and mixed secondary rainforests in West Malaysia. Southeast Asian J Trop Med Public Health. 1972;3(4):605–12.

    Google Scholar 

  18. 18.

    Zahedi M, Jeffery J, Krishnasamy M, Bharat V. Ectoparasites fauna of Rattus rattus diardii from an urban and semi urban environment. J Malays Soc Health. 1984;4(2):25–7.

    Google Scholar 

  19. 19.

    Ho TM, Nadchatram M, Salleh I. Ectoparasites of rodents and bats from Taman Negara. Trop Biomed. 1985;2:193–5.

    Google Scholar 

  20. 20.

    Mariana A, Ho T, Saleh I, Indudharan R. Species distribution of ticks in two localities in Kelantan. Trop Biomed. 1996;13:185–8.

    Google Scholar 

  21. 21.

    Paramasvaran S, Sani RA, Hassan L, Krishnasamy M, Jeffery J, Oothuman P, et al. Ectoparasite fauna of rodents and shrews from four habitats in Kuala Lumpur and the states of Selangor and Negeri Sembilan, Malaysia and its public health significance. Trop Biomed. 2009;26(3):303–11.

    CAS  PubMed  Google Scholar 

  22. 22.

    Madinah A, Fatimah A, Mariana A, Abdullah MT. Ectoparasites of small mammals in four localities of wildlife reserves in Peninsular Malaysia. Southeast Asian J Trop Med Public Health. 2011;42(4):803–13.

    CAS  PubMed  Google Scholar 

  23. 23.

    Traub R. Malaysian Parasites XVII. Four new species of fleas (Siphonaptera). Stud Inst Med Res Fed Malaya. 1957;28:35–64.

    Google Scholar 

  24. 24.

    Traub R. Muesebeckella, a new genus of flea from New Guinea, with notes on convergent evolution (Siphonaptera: Pygiopsyllidae). Entomol Soc Wash Proc. 1969;71:374–96.

    Google Scholar 

  25. 25.

    Domrow R. Seven new species of Laelaps from Malaysia(Acarina: Laelaptidae). Acarologia. 1962;4:503–19.

    Google Scholar 

  26. 26.

    Audy J. Laurentella, a new subgenus of trombiculid mites, with notes on biology and medical importance. Bull Raffles Mus. 1956;28:5–26.

    Google Scholar 

  27. 27.

    Audy J. Malayan trombiculid mites. 2. Naked-eye observations on attached chiggers, with a simple checklist of Malayan species, and details of preferred hosts. Bull Raffles Mus. 1956;28:86–101.

    Google Scholar 

  28. 28.

    Johnson PT. The Hoplopleurid lice of the Indo-Malayan subregion (Anoplura: Hoplopleuridae). Entomol Soc Am. 1964;4(3):68–102.

    Google Scholar 

  29. 29.

    Keirans JE, Clifford GM, Hoogstraal H. Description of the male and immature stages of Ixodes werneri Kohls (Acarina: Ixodidae), a parasite of Rattus in mountains of Palawan, Malaya and Java. J Med Entomol. 1970;7(5):605–8.

    CAS  PubMed  Article  Google Scholar 

  30. 30.

    Hubert AA, Baker HJ. Studies on the habitats and population of Leptotrombidium (Leptotrombidium) akamushi and L. (L) deliensis in Malaya (Acarina: Trombiculidae). Trop Med Hyg. 1963;78(2):131–42.

    CAS  Google Scholar 

  31. 31.

    Upham RW, AA JH, Phang OW, Yusof BM, Rapmund G. Distribution of Leptotrombidium (Leptotrombidium) arenicola (Acarina: Trombiculidae) on the ground in West Malaysia. J Med Entomol. 1971;8(4):401–6.

    PubMed  Article  Google Scholar 

  32. 32.

    Roberts LW, Muul I, Robinson DM. Numbers of Leptotrombidium (Leptotrombidium) deliense (Acarina: trombiculidae) and prevalence of Rickettsia tsutsugamushi in adjacent habitats of peninsular Malaysia. Southeast Asian J Trop Med Public Health. 1977;8(2):207–13.

    CAS  PubMed  Google Scholar 

  33. 33.

    Marchette NJ. Rickettsioses (Tick typhus, Q-fever, Urban Typhus) in Malaya. J Med Entomol. 1966;2(4):339–71.

    CAS  PubMed  Article  Google Scholar 

  34. 34.

    Brown G, Dohany A, Shirai A, Gan E, Huxsoll D. Murine typhus in a Malaysian village. Southeast Asian J Trop Med Public Health. 1977;8(1):99–103.

    CAS  PubMed  Google Scholar 

  35. 35.

    Dohany A, Lim B, Robinson D, Huxsoll D. An ecological study of Rickettsia tsutsugamushi in the primary forest of Taman Negara, Peninsular Malaysia. J Med Entomol. 1980;17(1):35–9.

    Article  Google Scholar 

  36. 36.

    Nadchatram M. Malaysian Parasites-XLVIII- Notes on rearing Malayan ixodid ticks (Acarina: Ixodidae) with special reference to Ixodes granulatus and Amblyomma geoemydae (Cantor). Stud Inst Med Res Fed Malaya. 1960;29:217–24.

    Google Scholar 

  37. 37.

    Nadchatram M. Correlation of habitat, environment and color of chiggers, and their potential significance in the epidemiology of scrub typhus in Malaya (Prostigmata: Trombiculidae). J Med Entomol. 1970;7(2):131–44.

    CAS  PubMed  Article  Google Scholar 

  38. 38.

    Harrison JL. Malaysian parasites-X-Feeding times of trombiculid mites. Stud Inst Med Res. 1953;26:171–83.

    Google Scholar 

  39. 39.

    Kundin WD, Nadchatram M, Keong A. Notes on the duration of attachment of certain Malaysian trombiculid mite larvae. J Med Entomol. 1972;9(6):558–9.

    CAS  PubMed  Article  Google Scholar 

  40. 40.

    Audy J, Harrison J. Malaysian parasites- I- collections made in Malaya by the Colonial Office Scrub Typhus research unit. Stud Inst Med Res. 1953;26:1–22.

    Google Scholar 

  41. 41.

    Shabrina MS, Saharudin A, Refaee H. Ectoparasite of small mammals from Bukit Lanjan and Air Hitam Forest Reserves, Selangor. J Wildlife Parks. 1989;8:31–4.

    Google Scholar 

  42. 42.

    Gater BAR. Malayan Trombidid Larvae Part I. (Acrina: Trombididiidae) with descriptions of seventeen new species. Parasitology. 1932;24:143–74.

    Article  Google Scholar 

  43. 43.

    Nur Syazana MT, Mohd Zain SN, Jeffery J. Biodiversity and macroparasitic distribution of the wild rat population of Carey Island, Klang. Trop Biomed. 2013;30(2):199–210.

    CAS  Google Scholar 

  44. 44.

    Madinah A, Abang F, Mariana A, Abdullah M, Mohd-Azlan J. Interaction of ectoparasites-small mammals in tropical rainforest of Malaysia. Community Ecol. 2014;15(1):113–20.

    Article  Google Scholar 

  45. 45.

    Philip CB, Traub R, Smadel JE. Chloramphenicol (Chloromycetin) in the chemoprophylaxis of scrub typhus (tsutsugamushi disease) I- epidemiological observations on hyperendemic areas of scrub typhus in Malaya. Am J Trop Med Hyg. 1949;50:63–74.

    CAS  Google Scholar 

  46. 46.

    Audy J, Harrison J. A review of investigations of mite typhus in Burma and Malaya, 1945–1950. Trans R Soc Trop Med Hyg. 1951;44(4):371–404.

    CAS  PubMed  Article  Google Scholar 

  47. 47.

    Nadchatram M. Natural History of the Genus Leptotrombidium (Acari: Trombiculidae) with Reference to Scrub Typhus. In: Griffiths DA, Bowman CE, editors. Acarology VI. Chichester, England: Ellis Horwood Limited; 1984. p. 1107–13.

    Google Scholar 

  48. 48.

    Audy J, Womersley H. Malaysian parasites, XXIX. New species of Oriental and Australian Trombiculidae (Acarina). Stud Inst Med Res. 1957;28:359–82.

    Google Scholar 

  49. 49.

    Traub R, Evans TM. Stud Inst Med Res. 1957;28:297–358.

    Google Scholar 

  50. 50.

    Traub R, Morrow ML. Malayan Parasites-XXV- Descriptions and records of some Southeast Asian chiggers of the genus Gahliepia (Acarina: Trombiculid). Stud Inst Med Res. 1957;28:169–86.

    Google Scholar 

  51. 51.

    Traub R, Wisseman CL. The ecology of chigger-borne rickettsiosis (scrub typhus). J Med Entomol. 1974;11(3):237–303.

    CAS  PubMed  Article  Google Scholar 

  52. 52.

    Grokhovskaya I, Huan-Hoe N. Gamasid mites of North Vietnam, Part 2. Zool Zhurnal. 1961;40:1633–46.

    Google Scholar 

  53. 53.

    Baker E, Traub R, Evans T. Indo-Malayan Haemolaeiaps, with descriptions of new species (Acarina: Laelaptidae). Pac Insects. 1962;4(1):91–100.

    Google Scholar 

  54. 54.

    Mariana A, Kulaimi BM, Halimaton I, Ho TM, Saleh I, Zuraidawati Z, et al. 6 Siri Kepelbagaian Biologi Hutan. In: Shahruddin MI, editor. Fauna ektoparasite di altitud berbeza di Hutan Simpan Gunung Jerai, Kedah: Pengurusan, Persekitaran Fizikal dan kepelbagaian Biologi. Malaysia: Jabatan Perhutanan Semenanjung Malaysia; 2006.

    Google Scholar 

  55. 55.

    Mariana A, Zuraidawati Z, Ho TM, Kulaimi BM, Saleh I, Shukor MN, et al. 7 Siri Kepelbagaian Hutan. In: Huda A, editor. Gunung Mandi Angin, Terengganu: Pengurusan, Persekitaran Fizikal, Kepelbagaian Biologi & Pelancongan Ekologi. Malaysia: Jabatan Perhutanan Semenanjung Malaysia; 2006.

    Google Scholar 

  56. 56.

    Fain A. Classification and geographical distribution of Listrophoroide. Proc 3rd Int Congr Acarol. 1971;1971:411–4.

    Google Scholar 

  57. 57.

    Fain A, Lukoschus F, Nadchatram M. Malaysian parasitic mites II. Myobiidae (prostigmata) from rodents. Int J Acarol. 1980;6:109–20.

    Article  Google Scholar 

  58. 58.

    Traub R. Notes on Indo-Malayan fleas, with descriptions of new species. Proc Entomol Soc Wash. 1950;52:109–43.

    Google Scholar 

  59. 59.

    Singh KI. Survey for vectors of plague at the refugee camp, Pulau Bidong, Kuala Terengganu. J Malays Soc Health. 1990;8(1):43–6.

    Google Scholar 

  60. 60.

    Zahedi M, Jeffery J, Krishnasamy M, Bharat V. Ectoparasites of Rattus rattus diardii from Kuala Lumpur City Malaysia. Proc 2nd Int Conf Urban Pest. 1996;1996:437–9.

    Google Scholar 

  61. 61.

    Audy J, Nadchatram M, Lim B. Malaysian parasites – XIIX – host distribution of Malayan ticks (Ixodoidea). Stud Inst Med Res Malaya. 1960;29:225–46.

    Google Scholar 

  62. 62.

    Hoogstraal H, Trapido H, Kohls GM. Southeast Asian Haemaphysalis ticks (Ixodoidea, Ixodidae). H.(Kaiseriana) papuana nadchatrami spp. n. and redescription of H.(K.) semermis Neumann. J Parasitol. 1965;51(3):433–51.

    CAS  PubMed  Article  Google Scholar 

  63. 63.

    Trevor NP, James EK. Ticks of the genus Ixodes in South-east Asia. Trop Biomed. 1994;11:123–34.

    Google Scholar 

  64. 64.

    Johnson PT. Some Anoplura of the Oriental region. A study of Hoplopleura pacifica Ewing and allies. J Med Entomol. 1972;9(3):219–27.

    CAS  PubMed  Article  Google Scholar 

  65. 65.

    Madinah A, Mariana A, Fatimah A, Abdullah M. Research Note A preliminary field survey of ectoparasites of rodents in urban park, Sarawak, Malaysian Borneo. Trop Biomed. 2013;30(3):1–5.

    Google Scholar 

  66. 66.

    Harrison JL. The effects of withdrawal of the host on populations of trombiculid mites. Bull Raffles Mus. 1956;28:112–9.

    Google Scholar 

  67. 67.

    Nadchatram M, Ramalingam S. Dermatitis caused by Ornithonyssus bacoti (Hirst, 1913). Southeast Asian J Trop Med Public Health. 1974;5(1):150.

    Google Scholar 

  68. 68.

    Nadchatram M. The Gunong Benom Expedition, 1967. 9. A collection of chiggers from Gunong Benom (Prostigmata: Trombiculidae). Bull Br Mus Nat Hist Zool. 1972;4:189–98.

    Google Scholar 

  69. 69.

    Traub R, Frick LP. Chloramphenicol (Chloromycetin) in the chemoprophylaxis of scrub typhus (Tsutsugamushi Disease) V. Relation of number of vector mites in hyperendemic areas to infection rates in exposed volunteers. Am J Epidemiol. 1950;51:242–7.

    Google Scholar 

  70. 70.

    Traub R, Wisseman Jr CL. Ecological considerations in scrub typhus: 1. Emerging concepts. Bull World Health Organ. 1968;39(2):209–18.

    PubMed Central  CAS  PubMed  Google Scholar 

  71. 71.

    Lim B-L. Distribution, relative abundance, food habits, and parasite patterns of giant rats (Rattus) in West Malaysia. J Mammal. 1970;51(4):730–40.

    CAS  PubMed  Article  Google Scholar 

  72. 72.

    Muul I, Liat L. Habitat distribution and ectoparasites of small mammals in Sarawak. Sarawak Mus J. 1974;20(1972):359–66.

    Google Scholar 

  73. 73.

    Syed-Arnez ASK, Mohd Zain SN. A study on wild rats and their endoparasite fauna from the Endau Rompin National Park, Johor. Malays J Sci. 2006;25(2):19–39.

    Google Scholar 

  74. 74.

    Mohr CO. Relation of ectoparasite load to host size and standard range. J Parasitol. 1961;47:978–84.

    CAS  PubMed  Article  Google Scholar 

  75. 75.

    Harrison JL. The effects of grassfire on populations of trombiculid mites. Bull Raffles Mus. 1956;28:102–11.

    Google Scholar 

  76. 76.

    Traub R, Wisseman CL. Diminution in the endemicity of scrub typhus with the passage of time. J Med Entomol. 1972;9(6):590–1.

    Google Scholar 

  77. 77.

    Hoogstraal H, Lim BL, Nadchatram M, Anastos G. Gunong Benom Expedition, 1967. 8. ticks (Ixodidae) of Gunong Benom and their altitudinal distribution, hosts and medical relationships. Bull Br Mus Nat Hist Zool. 1972;23:167–86.

    Google Scholar 

  78. 78.

    Brown GW, Robinson DM, Huxsoll DL. Serological evidence for a high incidence of transmission of Rickettsia tsutsugamushi in two Orang Asli settlements in Peninsular Malaysia. Am J Trop Med Hyg. 1978;27(1):121–4.

    CAS  PubMed  Google Scholar 

  79. 79.

    Brown GW, Shirai A, Jegathesan M, Burke DS, Twartz JC, Saunders JP, et al. Febrile illness in Malaysia–an analysis of 1,629 hospitalized patients. Am J Trop Med Hyg. 1984;33(2):311–5.

    CAS  PubMed  Google Scholar 

  80. 80.

    Tay ST, Muniady K, AS K, SC S, Ho TM, Rohani MY, et al. Seroepidemiologic survey of Orientia tsutsugamushi, Rickettsia typhi, and TT118 spotted fever group rickettsiae in rubber estate workers in Malaysia. Am J Trop Med Hyg. 1999;61(1):73–7.

    Google Scholar 

  81. 81.

    Traub R, Wisseman Jr CL. Ecological considerations in scrub typhus: 2. Vector species. Bull World Health Organ. 1968;39(2):219–30.

    PubMed Central  CAS  PubMed  Google Scholar 

  82. 82.

    Rapmund G, Dohany AL, Manikumaran C, Chan TC. Transovarial transmission of Rickettsia tsutsugamushi in Leptotrombidium (Leptptrombidium) arenicola Traub (Acarina: Trombiculidae). J Med Entomol. 1972;9(1):71–2.

    CAS  PubMed  Article  Google Scholar 

  83. 83.

    Lewthwaite R, Hodgkin EP, Savoor SR. The Typhus Group of Diseases in Malaya. Part VI: The Search for Carriers. Br J Exp Pathol. 1936;17(4):309–17.

    PubMed Central  Google Scholar 

  84. 84.

    Tay ST, Ho TM, Rohani MY, Devi S. Antibodies to Orientia tsutsugamushi, Rickettsia typhi and spotted fever group rickettsiae among febrile patients in rural areas of Malaysia. Trans R Soc Trop Med Hyg. 2000;94(3):280–4.

    CAS  PubMed  Article  Google Scholar 

  85. 85.

    Audy J. The role of mite vectors in the natural history of scrub typhus. Proc 10th Int Congr Entomol. 1958;3:639–49.

    Google Scholar 

  86. 86.

    Cadigan Jr FC, Andre RG, Bolton M, Gan E, Walker JS. The effect of habitat on the prevalence of human scrub typhus in Malaysia. Trans R Soc Trop Med Hyg. 1972;66(4):582–7.

    PubMed  Article  Google Scholar 

  87. 87.

    Robinson DM, Gan E, Donaldson JR. The prevalence of scrub typhus antibodies in residents of West Malaysia. Trop Geogr Med. 1976;28(4):303–8.

    CAS  PubMed  Google Scholar 

  88. 88.

    Turner RW, Martoprawiro S, Padmowiryono SA. Dynamics of the plague transmission cycle in Central Java (Ecology of potential flea vectors). Bull Health Res Indones. 1974;2(2):15–37.

    Google Scholar 

  89. 89.

    Hadi TR, Sarbini S, Dennis DT. Survey of small mammal ectoparasites in West Sumatra, Indonesia. Southeast Asian J Trop Med Public Health. 1981;12(2):275–7.

    Google Scholar 

  90. 90.

    Elbel RE, Thaineua M. A flea and rodent control program for plague prevention in Thailand. Am J Trop Med Hyg. 1957;6:280–93.

    CAS  PubMed  Google Scholar 

  91. 91.

    Olson WP. Rat-flea indices, rainfall, and plague outbreaks in Vietnam, with emphasis on the Pleiku area. Am J Trop Med Hyg. 1969;18(4):621–8.

    CAS  PubMed  Google Scholar 

  92. 92.

    Brooks J, Naing UH, Walton D, Myint D, Tun U, Thaung U, et al. Plague in small mammals and humans in Rangoon, Burma. Southeast Asian J Trop Med Public Health. 1977;8(3):335–44.

    CAS  PubMed  Google Scholar 

  93. 93.

    Tay ST, Mokhtar AS, Zain SNM, Low KC. Isolation and molecular identification of bartonellae from wild rats (Rattus Species) in Malaysia. Am J Trop Med Hyg. 2014;90(6):1039–42.

    PubMed  Article  Google Scholar 

  94. 94.

    Smith CEG. A virus resembling Russian spring-summer encephalitis virus from an ixodid tick in Malaya. Nature (Lond). 1956;178:581–2.

    CAS  Article  Google Scholar 

  95. 95.

    Tan SK, Smith CEG, Mcmahon DA, Bowen ETW. Lanjan virus, a new agent isolated from Dermacentor auratus in Malaya. Nature. 1967;214:1154–5.

    CAS  PubMed  Article  Google Scholar 

  96. 96.

    Hoogstraal H, Wassef HY. Dermacentor (Indocentor) auratus (Acari: Ixodoidea: Ixodidae): hosts, distribution, and medical importance in tropical Asia. J Med Entomol. 1985;22(2):170–7.

    CAS  PubMed  Article  Google Scholar 

  97. 97.

    Sandosham AA, Nordin K. Animal parasites of animals which affect man in Malaysia. Med J Malaya. 1967;22(1):16–25.

    Google Scholar 

  98. 98.

    Saleh I, Ho TM. Ectoparasites of some laboratory animals. Trop Biomed. 1989;6:113–6.

    Google Scholar 

  99. 99.

    Boese JL. Tissue reactions at the site of attachment of chiggers. J Med Entomol. 1972;9(6):591.

    Google Scholar 

  100. 100.

    Audy J, Vercammen-Grandjean P. Endoparasitism in trombiculid mites. Nature. 1955;175:263–4.

    CAS  PubMed  Article  Google Scholar 

  101. 101.

    Nadchatram M, Domrow R. The intranasal species of Lauremtella. J Med Entomol. 1964;1(1):29–39.

    CAS  PubMed  Article  Google Scholar 

  102. 102.

    Zhang RL, Zhang B. Prospect of using DNA barcoding for species identification and evaluation of the accuracy of sequence databases for ticks (Acari: Ixodida). Ticks Tick Borne Dis. 2014;5:352–8.

    PubMed  Article  Google Scholar 

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The authors would like to thank the University of Malaya for the support of this work.

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Correspondence to Siti Nursheena Mohd Zain.

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The authors declare that they have no competing interests.

This review was put together with the assistance of The University Malaya Research Grants FS285/2008C and FP005/2008C.

Authors’ contributions

SNMZ and SAASK performed the systematic literature review. SNMZ conceived and wrote the review with assistance from JJ, NMAA, JJW, KAB, and PS. All authors read and approved the final manuscript.

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Mohd Zain, S.N., Syed Khalil Amdan, S.A., Braima, K.A. et al. Ectoparasites of murids in peninsular Malaysia and their associated diseases. Parasites Vectors 8, 254 (2015).

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  • Ectoparasites
  • Host distribution
  • Mites
  • Ticks
  • Fleas
  • Lice