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Table 2 Results of technical reproducibility using serum from different experimentally infected animals

From: Development of a multiplex fluorescence immunological assay for the simultaneous detection of antibodies against Cooperia oncophora, Dictyocaulus viviparus and Fasciola hepatica in cattle

 

Animal 1

Animal 2

Animal 3

Animal 4

Animal 5

C. oncophora

     

N

8

8

8

8

8

Mean MFI value

2236

3778

3870

3124

2876

CV

3.29 %

2.11 %

3.33 %

4.78 %

6.12 %

D. viviparus

     

N

12

8

6

6

6

Mean MFI value

2332

3738

2595

1923

1472

CV

4.47 %

5.32 %

17.24 %

24.88 %

9.58 %

F. hepatica

     

N

12

10

8

  

Mean MFI value

1583

1888

4770

  

CV

9.39 %

9.64 %

3.09 %

  
  1. N number of replicates
  2. MFI median fluorescence intensity
  3. CV coefficient of variation