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Fig. 3 | Parasites & Vectors

Fig. 3

From: Development of a LAMP assay for detection of Leishmania infantum infection in dogs using conjunctival swab samples

Fig. 3

Specificity of L. infantum LAMP. Total DNA (100 ng) from different Leishmania species and strains were amplified by LAMP using primers targeting L. infantum MCAN/CN/90/SC kinetoplast minicircle DNA and by conventional PCR using Leishmania genus-specific primers. a Visual appearance of LAMP products. b Agarose gel electrophoresis of LAMP products. c Agarose gel electrophoresis of PCR products. Lane M, 100 bp ladder; lane 1, L. infantum MCAN/CN/90/SC; lane 2, L. donovani MHOM/IN/80/DD8; lane 3, L. major MHOM/SU/75/5ASKH; lane 4, L. tropica MHOM/SU/73/K27; lane 5, L. braziliensis MHOM/BR/75/M2903; lane 6, L. infantum MHOM/TN/80/IPT1; lane 7, water (negative control)

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