Fig. 3From: Development of a LAMP assay for detection of Leishmania infantum infection in dogs using conjunctival swab samplesSpecificity of L. infantum LAMP. Total DNA (100Â ng) from different Leishmania species and strains were amplified by LAMP using primers targeting L. infantum MCAN/CN/90/SC kinetoplast minicircle DNA and by conventional PCR using Leishmania genus-specific primers. a Visual appearance of LAMP products. b Agarose gel electrophoresis of LAMP products. c Agarose gel electrophoresis of PCR products. Lane M, 100Â bp ladder; lane 1, L. infantum MCAN/CN/90/SC; lane 2, L. donovani MHOM/IN/80/DD8; lane 3, L. major MHOM/SU/75/5ASKH; lane 4, L. tropica MHOM/SU/73/K27; lane 5, L. braziliensis MHOM/BR/75/M2903; lane 6, L. infantum MHOM/TN/80/IPT1; lane 7, water (negative control)Back to article page