First report of the invasive mosquito species Aedes koreicus in the Swiss-Italian border region
© Suter et al. 2015
Received: 19 January 2015
Accepted: 16 July 2015
Published: 30 July 2015
In 2012 and 2013, an entomological survey of Aedes albopictus, the Asian tiger mosquito, was carried out in the border region of southern Switzerland and northern Italy, using ovitraps. In July 2013, besides A. albopictus already known to the region several unusual eggs were recovered.
A total of 548 seemingly different eggs were found within three communities: Chiasso (Switzerland), and Como and Brunate (Italy). Proteomic diagnostics based on matrix-assisted laser desorption/ionization mass-spectrometry (MALDI-TOF MS) and morphological identification of one reared adult revealed the presence of at least 18 A. (Finlaya) koreicus (Edwards, 1917) specimens. A. koreicus is a species native to Southeast Asia and is competent to transmit Japanese encephalitis and potentially other arboviruses, as well as the dog heartworm Dirofilaria immitis. While new to Switzerland, this invasive species has previously been reported from Belgium, north-eastern Italy and European Russia.
This is the first report of the introduction of this exotic mosquito species into Switzerland and Lombardy, Italy, suggesting the range of A. koreicus is expanding in Central Europe. As A. koreicus is competent to vector pathogens its establishment imposes a risk to public and veterinary health. From a technical point of view, the presence of A. koreicus alongside A. albopictus requires careful analysis and reliable diagnostics. As a diagnostic tool the use of the recently developed MALDI-TOF MS approach has proofed to be a very useful approach, particularly since hatching rates of A. koreicus seem to be low, making identification by classic morphology difficult, if not impossible.
After the introduction and establishment of the Asian tiger mosquito, Aedes albopictus (Skuse), in the Swiss Canton of Ticino in 2003, an entomological surveillance and control programme was instigated by the canton’s mosquito working group, Gruppo cantonale di Lavoro Zanzare (GLZ) . GLZ uses ovitraps for the surveillance of A. albopictus as a basis for interventions in urbanised areas where the mosquito has become endemic . Intervention is chiefly based on larval source reduction through public awareness campaigns that include the distribution of leaflets, a hotline and a website, and by larviciding using diflubenzuron and Bacillus thuringiensis var. israeliensis (Bti), targeting small water containers (i.e. < 200 L) such as catch basins, plant saucers, drums, buckets, tarpaulins, tyres and bathtubs . Larger water bodies were inspected for the presence of larvae using standard dippers (model 1132, BioQuip Products, Rancho Dominguez, USA). If immatures were present, the breeding sites were also included in the control approach.
Ovitraps comprised 1.5 L, black plastic flower pots (Ramona Hydro, Luwasa, Switzerland) filled with 1.2 L tap water. Three equally spaced holes were drilled 2 cm below the rim to prevent the traps from being flooded by rain. A wooden slat made of untreated beech wood, measuring 20 cm × 2.5 cm × 0.5 cm, was placed inside the pot so that it was partially submerged and partially stuck out of the water. To prevent the ovitraps from becoming potential larval habitats, larvicide granules of Bti (VectoBac®, Valent BioSciences, USA) were added. The slats, water and Bti were replaced biweekly. The slats were individually labeled and wrapped in cling film for transport and preservation.
Field-caught mosquito eggs identified as Aedes koreicus across the Swiss-Italian border in 2013
Total egg number
Suspected as A. koreicus
Confirmed as A. koreicus
45.819 N, 9.082E
45.822 N, 9.090E
45.796 N, 9.087E
45.817 N, 9.086E
45.829 N, 9.033E
In the laboratory, the wooden slats collected from the ovitraps in Switzerland were incubated in tap water in an attempt to hatch the eggs. The water was allowed to settle for 24 h before use. The eggs were incubated for 7 days in a climate-controlled chamber (KBWF 720 E5.2, Binder GmbH, Germany) at a temperature of 28 °C, a relative humidity of 70 % and a light:dark cycle of 16:8 h. Unfortunately, only one larva hatched from which a female imago emerged. The specimen was morphologically identified as A. (Finlaya) koreicus (Edwards, 1917) using the taxonomic key of Ree , and later confirmed by Francis Schaffner (pers. comm.), a renowned expert in mosquito taxonomy. The identified female corresponded to the morphological form known from the South Korean volcanic island Jeju-do . This form has also recently been reported from Belgium  and north-eastern Italy .
Given the low hatching rate, a subsample of two to six eggs per slat (Table 1) were tested by matrix-assisted laser desorption/ionization mass-spectrometry (MALDI-TOF MS) in combination with a validated database, curated at Mabritec SA (Riehen, Switzerland) [8, 9]. Eggs selected for analysis were deposited close to each other and at a maximum distance from the eggs suspected to be A. albopictus. For the MALDI-TOF MS analysis the eggs were carefully detached from the slat with a brush and then prepared and processed as described by Schaffner et al. . All selected egg specimens were determined as A. koreicus (n = 17, Table 1).
Discussion and conclusions
A. koreicus was originally found in Korea, Japan, China and Eastern Russia . Its introduction has previously been reported from Belgium in 2008 , north-eastern Italy in 2011  and European Russia in 2013 . Meanwhile, the mosquito species has successfully established local populations in Belgium and north-eastern Italy [6, 11], confirming its ability to colonise new areas in temperate regions. Although in nature A. koreicus breeds in rock pools and tree holes, it successfully utilises artificial breeding sites in more urban environments, very much like other invasive Aedes species, including A. albopictus and A. japonicus (Theobald). Host seeking females may feed on humans and domestic animals both during the day and at night . At the end of the annual season when daylight becomes shorter, A. koreicus, like other members of the Aedes group, deposits eggs that are dormant and more resistant to desiccation and cold temperatures than eggs laid during the season. Dormant eggs catalyse the mosquito’s passive distribution to new areas . Capelli et al.  showed that A. koreicus dormant egg stages are even more resistant to cold temperatures compared to A. albopictus; and hence this species has the potential to colonise a much wider area of Switzerland and other parts of Europe.
Here, for the first time, A. koreicus was found in Switzerland and in the neighbouring Italian Lombardy region. It was not detected during the surveillance activities in 2012, but only in the 2013 summer season. As no A. koreicus eggs were found in 2012, we assume that this species has been introduced de novo although we cannot exclude that it was missed during the 2012 survey. While this is the first report of the introduction of this exotic mosquito species into Switzerland and the neighbouring Italian region, it suggests that the range of A. koreicus is generally expanding in Central Europe. Continued surveillance will show if this new invasive mosquito has established a local population and is gaining further ground.
Following the example of other invasive mosquito species, trade with used tyres and domestic plants has been suggested to be the route of entry [14–16]. As the single imaginal specimen from this study corresponds to the morphological form found in Belgium  and north-eastern Italy , these introductions might all be linked to the same mode of introduction, either at the regional or global level, or even both.
A. koreicus was suspected as a vector of Japanese encephalitis virus [17, 18] and of the dog heartworm Dirofilaria immitis . However, its full vector status is currently not resolved and requires further vector competence and field studies.
While the public health relevance of A. koreicus is still subject to debate, its presence in the southern part of Switzerland complicates the routine surveillance of A. albopictus. It is not possible to distinguish the eggs unambiguously by morphology through a stereo microscope. The eggs need either to be hatched out and reared to late developmental stages for species identification or analysed by technically more sophisticated tools such as DNA sequencing  or the MALDI-TOF MS approach applied here . In this study, the full cost per MALDI-TOF MS sample was 10 Euros. This is considerably lower than for PCR. The preparation of the samples is very simple and only takes a few minutes and the analysis itself takes only a few seconds. We appreciate that in a future study more specimens could be processed to gain a more complete picture of the situation.
The expansion and establishment of this, and potentially other, invasive mosquito species in Switzerland and Europe, as a whole, has to be observed carefully. It is important to implement methods that are able to detect a range of invasive species on a routine basis. As such, MALDI-TOF MS is a very useful tool for the identification of mosquito eggs, significantly simplifying surveillance and species-targeted interventions.
We thank Federica Apruti for her assistance in the field collections and Dr Francis Schaffner for sharing his expertise in mosquito species identification with us. We would also like to thank the population and communities of Albiolo, Bizzarone, Brunate, Cagno, Cavallasca, Caversaccio, Cernobbio, Como, Drezzo, Gironico, Lucino, Masliànico, Oligate Comasco, Paré, Rodero, Ronago, San Fermo della Battaglia, Uggiate-Trevano, and Arzo in Italy, Balerna, Breccio, Brusata, Caneggio, Chiasso, Civasca, Coldrerio, Colombera, Fontanella, Gasparina, Gorla, Ligornetto, Mendrisio, Morbio Inferiore, Novazzano, Obino, Pedrinate, Pignora, Prella, Rancate, Reseglia, Ronago, Ronco, Seseglio, Somazzo, Stabio and Tremona in Switzerland. We also thank two anonymous reviewers for their helpful comments on an earlier version of the manuscript. TS was financially supported by the Swiss-Brazilian Joint Research Programme (Grant no. BJRP 0112–08) and the Rudolf Geigy Foundation.
- Flacio E, Lüthy P, Patocchi N, Guidotti F, Tonolla M, Peduzzi R. Primo ritrovamento di Aedes albopictus in Svizzera. Boll Della Soc Ticinese Sci Nat. 2004;18:215–27.Google Scholar
- Flacio E, Engeler L, Tonolla M, Lüthy P, Patocchi N. Strategies of a thirteen years surveillance programme on Aedes albopictus (Stegomya albopicta) in southern Switzerland. Parasit Vectors. 2015;8:208.PubMed CentralPubMedView ArticleGoogle Scholar
- R Development Core Team. R: A Language and Environment for Statistical Computing. Vienna, Austria. URL http://www.r-project.org, 2010.
- Ree H-I. Taxonomic review and revised keys of the Korean Mosquitoes (Diptera: Culicidae). Entomol Res. 2003;33:39–52.View ArticleGoogle Scholar
- Tanaka K, Mizusawa K, Saugstad ES. A revision of the adult and larval mosquitoes of Japan (including the Ryukyu Archipelago and the Ogasawara Islands) and Korea (Diptera: Culicidae). Contrib Am Entomol Inst. 1979;16:vii 1–987.Google Scholar
- Versteirt V, De Clercq EM, Fonseca DM, Pecor J, Schaffner F, Coosemans M, et al. Bionomics of the established exotic mosquito species Aedes koreicus in Belgium, Europe. J Med Entomol. 2012;49:1226–32.PubMedView ArticleGoogle Scholar
- Capelli G, Drago A, Martini S, Montarsi F, Soppelsa M, Delai N, et al. First report in Italy of the exotic mosquito species Aedes (Finlaya) koreicus, a potential vector of arboviruses and filariae. Parasit Vectors. 2011;4:188.PubMed CentralPubMedView ArticleGoogle Scholar
- Schaffner F, Kaufmann C, Pflüger V, Mathis A. Rapid protein profiling facilitates surveillance of invasive mosquito species. Parasit Vectors. 2014;7:142.PubMed CentralPubMedView ArticleGoogle Scholar
- Müller P, Pflüger V, Wittwer M, Ziegler D, Chandre F, Simard F, et al. Identification of cryptic Anopheles mosquito species by molecular protein profiling. PLoS One. 2013;8:e57486.PubMed CentralPubMedView ArticleGoogle Scholar
- Bezzhonova OV, Patraman IV, Ganushkina LA, Vyshemirskiĭ OI, Sergiev VP. The first finding of invasive species Aedes (Finlaya) koreicus (Edwards, 1917) in European Russia. Med Parazitol (Mosk). 2014;1:16–9.Google Scholar
- Montarsi F, Martini S, Dal Pont M, Delai N, Ferro Milone N, Mazzucato M, et al. Distribution and habitat characterization of the recently introduced invasive mosquito Aedes koreicus (Hulecoeteomyia koreica), a new potential vector and pest in north-eastern Italy. Parasit Vectors. 2013;6:292.PubMed CentralPubMedView ArticleGoogle Scholar
- Mizusawa K, Saugstad ES. A revision of the adult and larval mosquitoes of Japan (including the Ryukyu Archipelago and the Ogasawara Islands) and Korea (Diptera: Culicidae). Contrib Am Entomol Inst. 1979;16:vii 1–987.Google Scholar
- Medlock JM, Hansford KM, Versteirt V, Cull B, Kampen H, Fontenille D, et al. An entomological review of invasive mosquitoes in Europe. Bull Entomol Res. 2015:1–27.Google Scholar
- Reiter P, Sprenger D. The used tire trade: a mechanism for the worldwide dispersal of container breeding mosquitoes. J Am Mosq Control Assoc. 1987;3:494–501.PubMedGoogle Scholar
- Schaffner F. Mosquitoes in used tyres in Europe: species list and larval key. Eur Mosq Bull. 2003;16:7–12.Google Scholar
- Scholte E-J, Jacobs F, Linton Y-M, Dijkstra E, Fransen J, Takken W. First record of Aedes (Stegomyia) albopictus in the Netherlands. Eur Mosq Bull. 2007;22:5–9.Google Scholar
- Miles JAR. Some ecological aspects of the problem of arthropod-borne animal viruses in the Western Pacific and South-East Asia regions. Bull World Health Organ. 1964;30:197–210.PubMed CentralPubMedGoogle Scholar
- Shestakov VI, Mikheeva AL. Contribution to study of Japanese encephalitis vectors in Primorye region. Med Parazit. 1966;35:545–50.Google Scholar
- Montarsi F, Ciocchetta S, Ravagnan S, Simonato G, Mutinelli F, Camuffo S, et al. Laboratory evidence on vector competence of the invasive mosquito Aedes koreicus [Hulecoeteomyia koreica] for Dirofilaria immitis. Parasit Vectors. 2014;7(Suppl 1):O34.PubMed CentralView ArticleGoogle Scholar
- Das B, Swain S, Patra A, Das M, Tripathy HK, Mohapatra N, et al. Development and evaluation of a single-step multiplex PCR to differentiate the aquatic stages of morphologically similar Aedes (subgenus: Stegomyia) species. Trop Med Int Health TM IH. 2012;17:235–43.PubMedView ArticleGoogle Scholar
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