Fig. 1From: Oxadiazole-2-oxides may have other functional targets, in addition to SjTGR, through which they cause mortality in Schistosoma japonicum Inhibition of recombinant SjTGR by oxadiazole-2-oxides. 50 nM SjTGR and 1 mM NADPH were incubated with different concentrations of oxadiazole-2-oxides (2, 4, 6, 10, 20 μM) for 15 min at room temperature. Then, potassium phosphate buffer (pH 7.4) containing 10 mM EDTA, 100 μM NADPH, 3 mM DTNB was added to start the reaction. The increase in absorbance at 412 nm was monitored during the first 2 min. All assays were done in triplicate. Results shown are the mean ± SD (n = 3)Back to article page