Fig. 2
Lesion size and parasite loads in wild type (WT) and gp91phox−/− mice infected with L. amazonensis. Mice were infected with 1 × 106 metacyclic promastigote forms of L. amazonensis in the right hind footpad and followed until 16 weeks. a Footpad thickness, **P < 0.01 between WT and gp91phox−/− mice. b Parasite loads in footpads of infected mice 4, 8, 12 and 16 weeks post-infection. c Parasite loads found in draining lymph nodes of infected mice 4, 8, 12 and 16 weeks post-infection. Data are shown as mean ± SD from one representative experiment of three, n = 5 for each experiment. d and e Inflammatory macrophages obtained from peritoneal cavity of C57BL/6 and gp91phox−/− mice were infected with L. amazonensis metacyclic promastigotes. The cells were washed to remove extracellular parasites and either fixed or re-incubated with medium for 72 h. The slides were stained and counted to determine the infection index. A minimum of 200 macrophages were counted per group in triplicate. d Infection index for C57BL/6 and gp91phox−/− macrophages four and 72 h post in vitro infection. e Infection index in macrophages from WT pre-incubated with N-acetyl-cysteine (NAC) or apocynin (APO) or from gp91phox−/− mice 72 h post in vitro infection