- Open Access
Impact of reactive oxygen species (ROS) on the control of parasite loads and inflammation in Leishmania amazonensis infection
© Roma et al. 2016
- Received: 5 November 2015
- Accepted: 23 March 2016
- Published: 7 April 2016
Reactive oxygen species (ROS) protect the host against a large number of pathogenic microorganisms. ROS have different effects on parasites of the genus Leishmania: some parasites are susceptible to their action, while others seem to be resistant. The role of ROS in L. amazonensis infection in vivo has not been addressed to date.
In this study, C57BL/6 wild-type mice (WT) and mice genetically deficient in ROS production by phagocytes (gp91phox−/−) were infected with metacyclic promastigotes of L. amazonensis to address the effect of ROS in parasite control. Inflammatory cytokines, parasite loads and myeloperoxidase (MPO) activity were evaluated. In parallel, in vitro infection of peritoneal macrophages was assessed to determine parasite killing, cytokine, NO and ROS production.
In vitro results show induction of ROS production by infected peritoneal macrophages, but no effect in parasite killing. Also, ROS do not seem to be important to parasite killing in vivo, but they control lesion sizes at early stages of infection. IFN-γ, TNF-α and IL-10 production did not differ among mouse strains. Myeloperoxidase assay showed augmented neutrophils influx 6 h and 72 h post - infection in gp91phox−/− mice, indicating a larger inflammatory response in gp91phox−/− even at early time points. At later time points, neutrophil numbers in lesions correlated with lesion size: larger lesions in gp91phox−/− at earlier times of infection corresponded to larger neutrophil infiltrates, while larger lesions in WT mice at the later points of infection also displayed larger numbers of neutrophils.
ROS do not seem to be important in L. amazonensis killing, but they regulate the inflammatory response probably by controlling neutrophils numbers in lesions.
- Leishmania amazonensis
Leishmaniasis are a spectrum of diseases caused by parasites of the genus Leishmania. This disease is endemic in 88 countries and affects two million people every year. The diseases may present themselves as cutaneous, mucocutaneous or visceral forms, depending on which species is involved in the infection .
In the well-established model of infection of mice with L. major, resistance to several parasite strains is mediated by the development of a Th1 immune response, while susceptibility is characterized by the development of a Th2 response [2–4], or to production of IL-10 . However, C57BL/6 and C57BL/10 mice, which completely heal infection with L. major, develop chronic non-healing lesions when infected with L. amazonensis [6–8]. In addition, this susceptibility is independent of a Th2 response .
Macrophages are the main host cell for Leishmania spp. When infected with these parasites, macrophages sustain Leishmania spp. growth. However, when activated to produce nitric oxide, macrophages can kill L. major [9, 10]. Activation of macrophages is dependent on IFN-γ and TNF [9, 10]. Macrophages infected with L. amazonensis produce less TNF, even in the presence of IFN-γ [11, 12]. Hence, activation of L. amazonensis infected macrophages is deficient, at least in vitro [11, 12].
Reactive oxygen species derive from oxygen reduction, generating a group of highly reactive ions, molecules and radicals. ROS may be generated in mitochondria as respiratory chain products  and also participate in many biological processes, such as hormonal biosynthesis , cellular signalling  and destruction of intracellular pathogens . ROS are also important effector agents against intracellular pathogens, induced by IFN-γ or Toll-like receptors [17, 18].
Phagocyte NADPH oxidases (NOX2) are a group of multimeric proteins composed by cytosolic chains (p67 phox, p47 phox and p40 phox), a small G protein (rac1 or rac2) and membrane-associated subunits (gp91 phox and p22 phox) . As other isoforms of NADPH oxidases, NOX2 catalyzes the production of superoxide anion (O2 ●−) by reducing oxygen, using NADPH as the electron donor . The resulting superoxide may generate many reactive species including oxidized halogens, oxygen singlet and other free radicals. Phagocytic cells use these oxidants to kill intracellular pathogens, but these species can also cause tissue damage to host cells. Hence, NOX2 is strictly regulated and is activated upon specific stimuli, such as phagocytosis triggered by pathogen-associated molecular patterns (PAMPS) .
Gp91phox is essential for NOX2 function. It is responsible for molecular oxygen reduction by electrons provided by NADPH . Humans or mice deficient in gp91phox present X-linked chronic granulomatous disease (CGD) [23, 24]. Patients with CGD have recurrent infections that can cause death as early as childhood. Although chemotaxis, degranulation and phagocytosis are normal, CGD patients show deficiency in destruction of phagocytosed microorganisms due to lack of metabolites generated from superoxide . Accordingly, gp91phox knockout mice eventually develop CGD. These mice respond to chemically induced peritonitis with extensive neutrophil infiltration , and increased secretion of inflammatory cytokines and chemokines during lung infection by pneumococcal pneumonia .
The effect of ROS in in vivo infection caused by Leishmania spp. has been less well studied, since nitric oxide is believed to be the major effector molecule involved in parasite killing [27, 28]. In vitro studies show an irrelevant role of ROS in parasite killing by macrophages infected with L. major [29, 30] and L. guyanensis . In vivo ROS control L. major parasitism in mice . In L. donovani infection, ROS would be important only for short-term control of the parasites . These differences in resistance to ROS observed during infection with different species of Leishmania make it necessary to investigate the role of ROS in other Leishmania spp. A few papers have addressed the role of ROS during infection with L. amazonensis. The role of ROS has been addressed in vitro by some authors by measuring the amount of ROS produced by macrophages infected with L. amazonensis [11, 33] or with L. pifanoi, a parasite belonging to the Mexicana complex, like L. amazonensis . ROS play a role in parasite killing of L. amazonensis by activated macrophages (with both IFN-γ and LPS) treated with ERK inhibitor . However, the role of ROS produced upon phagocytosis of L. amazonensis on parasite killing and during in vivo infection has not yet been addressed.
Mice and ethics statements
C57BL/6 mice were obtained from the animal house of the Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais (CEBIO). Mice which genes for gp91phox subunit of NADPH oxidase were deleted by homologue recombination (B6.129S-Cybbtm1Din/J, here named gp91phox−/−)  were purchased from Jackson Farms (Glensville, NJ, USA). Animals were kept in conventional conditions with barriers, controlled light cycle and temperature. Food and water were provided ad libitum. All animals used in this study were 6 to 12 week- old. This project was approved by the local ethical committee under the protocol CETEA 031/09.
Parasites, infections and generation of Leishmania antigen
Leishmania amazonensis (IFLA/BR/67/PH8) was maintained in Grace’s medium as previously described . Metacyclic promastigotes were purified in a ficoll gradient , washed, resuspended in phosphate buffered saline (PBS, pH 7.3) and counted. Inocula of 1 × 106 parasites/40 μl of PBS were injected in the mouse left hind footpad. Lesion development was followed by measuring the thickness of the footpad swelling using a digital micrometer (Starrett 727, Itu, SP, Brazil). Antigens were prepared from log phase promastigotes, washed in PBS and submitted to seven cycles of freezing in liquid nitrogen and thawing (37 °C). Suspensions were adjusted to a final concentration of 1 mg of protein/ml and kept at −70 °C until use. Protein concentration was assessed by the Lowry assay .
Quantification of parasites
Mice were sacrificed by cervical dislocation. The footpads were removed and disinfected in 70 % ethanol for 5 min and air - dried in the laminar flow hood. The footpads were cut in small parts and placed in RPMI medium (GIBCO, Grand Island, NY, USA) containing 100U/ml penicillin, 100 μg/ml streptomycin (GIBCO) and 125U/ml collagenase A (Sigma-Aldrich, Inc, St. Louis, MO, USA) for 2 h at 37 °C in a humidified chamber and atmosphere containing 5 % CO2. After incubation the pieces of footpads were ground, filtered with a 40 μm cell strainer filter (BD Falcon, Franklin Lakes, NJ, USA) and washed with 10 ml of RPMI 0.05 % DNAse (Sigma-Aldrich). The homogenates were centrifuged at 50 × g for 4 min to remove large tissue debris and the supernatants were collected and centrifuged at 1,500 × g for 15 min. The sediment was re-suspended in 1 ml of complete RPMI (GIBCO) (RPMI supplemented with 10 % heat-inactivated fetal bovine serum (FBS) (Cultilab, Campinas, SP, Brazil), 100U/ml penicillin, 100 μg/ml streptomycin and 2 mM l-glutamine (GIBCO BRL). Fifty microliters of the suspension were serially diluted in a 96-well plate containing 150 μl of Grace’s insect medium supplemented with 20 % heat-inactivated FBS, 100U/ml penicillin, 100 μg/ml streptomycin and 2 mM l-glutamine (GIBCO) (Grace’s complete medium) in each well. The samples were serially diluted in Grace’s complete medium (1:4) in triplicates. Pipette tips were discarded after each dilution. Plates were cultured for 10 days in BOD incubator at 25 °C and the last positive dilution was registered as the titre. Results are expressed as the negative logarithm of the titre.
Mice were injected intraperitoneally with 2 ml of 4 % thioglycollate (BD Biosciences, Franklin Lakes, NJ, USA). After 3 days, mice were euthanized and the peritoneum cells were harvested by repeated cycles of aspiration and re-injection with 10 ml of cold PBS in a 10 ml syringe with a 24G needle. Considering cell morphology and adherence, more than 80 % of the cells harvested were macrophages. Cells were centrifuged at 4 °C, 1,500 × g for 10 min, counted in a hemocytometer and the concentration was adjusted to 1×106 cells/100 μL of complete RPMI without phenol red. Cells (1 × 106 cells/well) were plated in 96 well opaque plates (NUNC, Rochester, NY, USA) together with 0.05 mM of luminol (5-amino-2,3-dihydro-1,4-phthalazinedione, Sigma-Aldrich). Immediately before the measurement, L. amazonensis metacyclic promastigotes were added in the proportion of 10 parasites per macrophage. The measurement was followed for 90 min with one minute of interval between the measurements. The production of ROS was assessed by the light intensity generated by the reaction between ROS and luminol and expressed as relative light units.
In vitro assays for parasite burden
Macrophages were isolated from the peritoneal cavity of mice 3 days after injection of 2 ml of 4 % thioglycollate medium (BD Biosciences, Franklin Lakes, NJ, USA) into the peritoneal cavity. After this time, mice were euthanized and the peritoneum cells were harvested by repeated cycles of aspiration and re-injection with 10 ml of cold PBS in a 10 ml syringe with a 24G needle. More than 80 % of the cells harvested were macrophages. The cells were centrifuged at 4 °C, 1,500 × g for 10 min and re-suspended in DMEM supplemented with 10 % fetal bovine serum (FBS) (Cultilab, Campinas, SP, Brazil), 1 % penicillin-streptomycin and 2 mM l-glutamine. Macrophages were counted in a hemocytometer prior to seeding 5×105 cells into each well of a 24-well plate and incubated at 37 °C, 5 % CO2 for 2 h. After this time, L. amazonensis metacyclic promastigotes were added in the proportion of 5 parasites per macrophage during 4 h. After this period, cells were washed three times with phosphate-buffered saline (PBS, pH 7.3) to remove extracellular parasites. Cells were fixed or reincubated with medium for 72 h before fixation with methanol. Coverslips with attached macrophages were stained with Panótico (Laborclin, Pinhais, PR, Brazil) and a minimum of 200 macrophages per coverslip were counted. The results were expressed as an infection index (% infected macrophages x number of amastigotes/total number of macrophages). The following drugs were used in these assays: apocynin (APO) (300 μM; Sigma-Aldrich) and N-acetyl-cysteine (NAC) (1 mM; Sigma-Aldrich) and H2O2 (100 μM). Drugs were added to the cells 2 h before and immediately after infection.
Real time PCR
Total RNA, obtained from lesions at 4, 8, 12 and 16 weeks post-infection, was extracted using Trizol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions. One μg of total RNA obtained from the lesions or lymph nodes was reverse transcribed using reverse transcriptase (Promega, Southampton, UK) and oligo (dT) 15-mer primer (Promega, Southampton, UK). PCR amplification was performed with a programmable thermal cycler (Perkin–Elmer 2400, Waltham, MA, USA). The cDNA amplification protocol was as follows: 2 min at 50 °C, activation of AmpliTaq at 95 °C for 10 min, melting at 95 °C for 15 s. For the annealing and final extension, the samples were heated at 60 °C for 1 min for 45 cycles. For dissociation curve, the samples were heated at 95 °C for 15 s, following cooling at 60 °C for 5 s. Finally, the samples were cooled for 1 min at 4 °C.
The amplification of cDNA was made using specific primers as follow: IFN-γ (forward TCAAGTGGCATAGATGTGGAAGAA, reverse TGGCTCTGCAGGATTTTCATG), IL-4 (forward ACAGGAGAAGGGACGCCA, reverse GAAGCCCTACAGACGAGCTCA), IL-10 (forward GGTTGCCAATTATCGGA, reverse ACCTGCTCCACTGCCTTGCT), TNF-α (forward TTCTGTCTACTGAACTTCGGGGTGATCGGTCC, reverse GTATGAGATAGCAAATCGGCTGACGGTGTGGG), IL1-β (forward CAACCAACAAGTGATATTCTCCAT, reverse GATCCACACTCTCCAGCTGCA), iNOS (forward CCCTTCCGAAGTTTCTGGCAGCAGC, reverse GGCTGTCAGAGCCTCGTGGCTTTGG). The reactions were developed in the ABI PRISM®7900HT (Applied Biosystems, Foster City, CA, USA) using 20 % of reaction in cDNA volume and 15 μl of total PCR mixture. All reactions were performed in duplicate using SYBR Green Master Mix (Applied Biosystems) according to manufacturer’s instructions. Finally, the samples were cooled for 1 min at 4 °C. The specific cDNAs were normalized according to the expression of ribosomal 18 s gene (forward TACCACATCCAAGAAGGCAG, reverse TGCCCTCCAATGGATCCTC) based in ΔCT calculation. The results were expressed as fold increase of target gene expression compared to ribosomal 18 s gene expression.
Cytokine and nitrite assays
Single cell suspensions of draining lymph nodes (dLNs) from mice infected for 8, 12 and 16 weeks post-infection plated in 24 well plates at 5 × 106 cells/mL (Nunclon, Nunc) and stimulated with L. amazonensis antigen (50 μg/ml) for 72 h at 37 °C and 5 % CO2. Concanavalin A was used as positive control (10 μg/ml) of cytokine production. The levels of IFN-γ, IL-10, IL-6, IL-17a were measured in supernatant using appropriate kits (BD OptEIATM, BD Biosciences, Franklin Lakes, NJ, USA) following manufacturer's instructions. Detection levels were 12.5 pg/ml for all cytokines measured.
Nitrites were measured in supernatants of peritoneal macrophage cultures infected with L. amazonensis. Briefly, peritoneal macrophages were harvested as described above. The cells (5 × 105) were plated in 48 well plates in 500 μl of complete RPMI. After 16 h, the cultures were washed to remove the non-adherent cells, and cells were activated with 50U/ml of IFN-γ (BD biosciences, Franklin Lakes, NJ, USA) and 100 ng/ml of LPS (Sigma-Aldrich) for 4 h at 37 °C and 5 % CO2. Following the activation, the cells were infected with L. amazonensis metacyclic promastigotes at 10 parasites per macrophage. After 4 h of infection, the cells were washed, re-stimulated with IFN-γ and LPS at the same concentrations used before and incubated for 48 h at 37 °C and 5 % CO2. The supernatants of in vitro infected macrophages were collected 48 h after infection and the levels of IL-6, TNF-α, IL-17A and CXCL-1, MCP-1 and IL-10 were measured using appropriate kits (BD OptEIATM, BD Biosciences, Franklin Lakes, NJ, USA) following manufacturer's instructions.
After 48 h, 50 μL of supernatants were collected and used to quantify nitrite using the Griess method  in 96 well plates. After 10 min of reaction the plates were read in a plate reader (EZ read 400, Biochrom, Cambridge, UK) at 540 nm.
Neutrophil accumulation in the infected footpads was measured by assaying myeloperoxidase (MPO) activity. Briefly, the footpads were infected as described in item 2.2. Six and 72 h post-infection, mice were euthanized, the footpads were removed and snap-frozen in liquid nitrogen. On thawing, the tissue (100 mg of tissue per 1.9 ml of buffer) was homogenized in pH 4.7 buffer (0.1 M NaCl, 0.02 M Na3PO4, 0.015 M sodium-ethylenediaminetetraacetic acid), centrifuged at 260 × g for 10 min and the pellet subjected to hypotonic lyses (15 ml of 0.2 % NaCl solution followed by 30 s of equal volume of a solution containing 1.6 % NaCl and 5 % glucose). After further centrifugation, the pellet was resuspended in 0.05 M sodium phosphate buffer (pH 5.4) containing 0.5 % hexadecyltrimethylammonium bromide and re-homogenized. One-ml aliquots of the suspension were transferred into 1.5 ml conical microtubes followed by three freeze-thaw cycles using liquid nitrogen. The aliquots were then centrifuged for 15 min at 10,000 × g, the pellet was re-suspended to 1 ml. Myeloperoxidase activity in the re-suspended pellet was assayed by measuring the change in optical density (OD) at 450 nm using tetramethylbenzidine (1.6 mM) and H2O2 (0.5 mM). Results were expressed as OD units.
Statistical significance between groups was determined by the unpaired, two-tailed Student’s t test using Prism software (GraphPad, La Jolla, CA, USA). P values < 0.05 were considered significant.
Leishmania amazonensis metacyclic promastigotes are capable of inducing a respiratory burst in mouse macrophages
Gp91phox−/− mice develop larger lesions after infection with L. amazonensis
To address the importance of ROS during L. amazonensis infection, we infected mice deficient in the gp91phox subunit of NOX2 (gp91phox−/−). This subunit is responsible for transferring electrons from NADPH to oxygen, generating superoxide anion. Consequently, gp91phox−/− mice cannot produce ROS through NOX2 .
Regardless of differences in lesion sizes observed between gp91phox−/− and WT mice, we found no differences in parasite loads, neither in lesions nor in draining lymph nodes, at 4, 8, 12 and 16 weeks post-infection (Fig. 2b, c). In addition, we found no differences between in vitro infection of WT and gp91phox−/− macrophages (Fig. 2d). Addition of apocynin, an inhibitor of NOX2, or N-acetyl-cysteine to WT macrophages confirmed that L. amazonensis is not susceptible to oxidative stress within macrophages (Fig. 2e).
Cytokine production in footpads, draining lymph nodes and in vitro-infected macrophages
Draining lymph node cells from gp91phox−/− mice produced more IL-17A 8 weeks post-infection than cells from WT mice. IL-17 production by dLN cells dropped at 12 and 16 weeks post-infection, and no differences were found between the groups (Fig. 4c). No differences in IL-17 mRNA were found in lesions (data not shown). Interestingly, despite the differences found in IL-1β mRNA expression levels 12 and 16 weeks post-infection in WT infected footpads (Fig. 3e), we could not observe augmented levels of this cytokine in dLNs of WT in all periods measured when compared to gp91phox−/− dLNs (Fig. 4d).
Deficiency in ROS production is not compensated by increase in iNOS expression
ROS and RNS may play complementary roles in killing intracellular parasites [32, 41]. However, in our experiments, gp91phox−/− mice harboured parasites similarly to WT. This could be due to an irrelevant role of ROS in infection with L. amazonensis or to a compensatory over-production of nitric oxide. We investigated the mRNA expression of inducible nitric oxide synthase (iNOS) in lesions and the production of nitric oxide (NO) by peritoneal macrophages in gp91phox−/− mice.
ROS influence the migration of neutrophils after infection with L. amazonensis
In this work, we analysed the importance of ROS production by the host on the outcome of infection caused by L. amazonensis. C57BL/6 mice are unable to spontaneously heal lesions after subcutaneous infection with L. amazonensis, but develop chronic lesions that do not progress indefinitely [6, 8]. We observed increase in paw swelling of infected WT mice until week 7 post-infection, followed by a slight decrease and lesion stabilization after this time point. Indeed, our results are supported by other works showing similar lesion kinetics during L. amazonensis infection in C57BL6 mice [42–44]. A role for IFN-γ and iNOS in this partial resistance to the parasite is clear, especially at later times of infection, since L. amazonensis-infected IFN-γ−/− and iNOS−/− mice develop larger non-healing lesions than wild type mice [44, 45]. ROS have been shown to play a role in the containment of metastasis to spleen and lymph nodes in experimental L. major infection . Macrophages [29, 30] and neutrophils  respond to infection with L. major with ROS production, as shown in vitro. However, killing of L. major by IFN-γ-activated macrophages is dependent on NO production, but not on the production of superoxide or peroxynitrite , hampering the role of ROS in resistance to this parasite. L. guyanensis amastigotes, on the other hand, die inside BALB/c macrophages through an apoptosis-like process mediated by parasite-induced ROS . However, little is known about the role of ROS in resistance to L. amazonensis. It has been reported that L. amazonensis triggers less ROS production by macrophages from CBA mice in vitro 30 min after infection . However, production of ROS by activated macrophages from C57BL/6 mice infected with L. amazonensis 30 min and 5 days post-infection has been reported . Production of ROS was dependent on gp91phox and ROS mediated parasite killing in vitro. In addition, in vivo ROS production by C57BL/6 mice during L. amazonensis infection has been reported [47, 48]. The production of ROS was determined indirectly by detection of nitrotyrosine, which provides evidence of peroxynitrite production. Peroxynitrite is produced by the reaction of ROS (produced by NADPH oxidase) and NO (produced by iNOS), these enzymes co-localize in the phagolysosome, speaking for their role in the production of peroxynitrite and damage to the parasite .
In our experiments, using metacyclic promastigote forms of PH8 L. amazonensis strain and thioglycollate-elicited macrophages from C57BL/6 mice, L. amazonensis induces ROS production by peritoneal macrophages in vitro (Fig. 1). Parasites of the genus Leishmania possess several mechanisms to escape ROS in phagolysosomes. L. pifanoi is capable of blocking the maturation of the gp91phox subunit of RAW 264.7 cells, avoiding the NADPH oxidase complex formation . L. donovani LPG blocks the translocation of p47phox and p67phox to the phagosome, inhibiting NADPH oxidase activation . L. amazonensis promastigotes developed strategies to resist to ROS; however, the mechanisms of this resistance have not been determined yet. It is possible that L. amazonensis does not inhibit NOX2, since L. amazonensis-infected CBA macrophages respond to infection with L. major similarly to uninfected macrophages . On the other hand, the host produces ROS in response to a variety of stimuli, such as activated complement , binding to IgG Fc receptor (FcγR)  and Toll-like receptor 2 , during Leishmania spp. infection. Considering the sophisticated mechanisms used by the parasites to escape damage by ROS and the variety of mechanisms by which the host achieves ROS production, allied with the susceptibility of some species of Leishmania to ROS , it was reasonable to infer that ROS may play a role in resistance to L. amazonensis. Lesion development in gp91phox−/− and WT mice after infection with L. amazonensis was quite different. In the first seven weeks of infection, gp91phox−/− mice presented larger lesions, but the same number of parasites as WT mice. Coherently, macrophages from WT and gp91phox−/− mice harboured similar numbers of parasites and produced similar levels of NO when activated with IFN-γ, and similar levels of message for iNOS were found in footpads from both infected mouse strains. In addition, cytokine levels at the site of infection, as well as IFN-γ, IL-1β and IL-10 production by antigen-stimulated lymph node cells were similar among groups. However, IL-17 production by draining lymph node cells was higher in gp91phox−/− mice than in WT at 8 weeks of infection. Interestingly, MPO activity was higher in gp91phox−/− footpads than in WT at 6 and 72 h post-infection. In addition, more Ly6G+ cells were found at the site of infection 8 weeks post-infection in gp91phox−/− mice, subsequently to when gp91phox−/− mice displayed larger lesions than WT mice. On the other hand, at 12 weeks of infection, when WT mice had larger lesions, they also had more neutrophils at the site of infection. These data suggest that ROS could have a relevant role in modulating the inflammatory infiltrate, but irrelevant action in parasite killing in vivo. The reason for the larger lesions up to 7 weeks (maybe coinciding with larger numbers of neutrophils) and smaller lesions at later time points (also coinciding with smaller numbers of neutrophils) is not yet known. Ablation of neutrophils early in infection with L. amazonensis, in our experiments, caused no effect in the outcome of infection in C57BL6 mice . However, our results here suggest that the persistence of this otherwise short-lived cell may exacerbate pathology. Larger neutrophil infiltrates are found in NOX2-deficient mice , and that would explain, possibly, the larger lesions at the earlier time points of infection. As stated above, at the later time points a larger neutrophil infiltrate was found in WT mice. The reason for the variation in these numbers in the two mice is unknown at this point. We could, however, speculate that the neutrophil infiltrate is more precocious in gp91phox−/− mice and more precociously resolved than in WT mice. Eventually, lesions were the same in both mouse strains.
Humans deficient on functional NADPH oxidase develop chronic granulomatous disease (CGD), which is characterized by recurrent infections and limitations in elimination of intracellular microorganisms; gp91phox−/− mice kept in less sanitary conditions may also develop CGD [23, 25]. Moreover, gp91phox−/− mice show high numbers of neutrophils during peritonitis caused by chemical agents  as well as increased inflammatory cytokine and chemokine production . There are solid evidences implicating ROS in the induction of neutrophil apoptosis [54–60]. Consequently, it is possible to infer that a decrease in neutrophil apoptosis culminates with the hyper-inflammation seen in CGD [56, 61–64]. Accordingly, at early time points (6 and 72 h, Fig. 7a) we observed higher numbers of neutrophils at the infection site in gp91phox−/− mice. This fact could be related with the impaired capacity of neutrophils from gp91phox−/− mice to start the apoptotic programme via ROS. Moreover, other mechanisms such as persistent cellular activation , attenuation of ROS dependent Ca2+ signaling [66, 67] and oxidation of transcriptional factors and phosphatases via ROS  could contribute to the larger lesions in gp91phox−/− mice. Despite extensive data in the literature reporting increased expression of inflammatory cytokines in gp91phox−/− mice [66, 67, 69, 70], we could not detect alterations in cytokine mRNA levels (Fig. 3a, b) in lesions. Nevertheless, macrophages infected in vitro with L. amazonensis and activated with IFN-γ produced higher levels of IL-6, TNF-α, IL-17A and IL-10 (perhaps in response to high levels of TNF-α ) and lower levels of MCP-1 when compared to cytokine production by WT macrophages. In addition, a larger production of IL-17A by lymph node cells was found at 8 weeks of infection, which correlated with the larger neutrophil infiltrate. Th17 response is related to increased neutrophil migration in many experimental models [72–75]. The higher production of IL-17A observed herein could contribute to the migration of neutrophils to lesions and promote a larger inflammatory infiltrate in gp91phox−/− mice. Surprisingly, after 11 weeks of infection, lesions in gp91phox−/− mice started to decrease and became smaller that lesions in the WT group. Again, no differences in parasite numbers were found between groups. However, the mRNA levels IL-1β were higher in WT mice at later time points, indicating an inflammatory status. Indeed, IL-1β expression could be dependent of ROS release , which could explain the decrease of footpad swelling and the low concentration mRNA levels of IL-1β observed in gp91phox−/− mice at this time point. Gp91phox−/− footpads expressed higher mRNA levels for IL-4, which could indicate an anti-inflammatory status at later times of infection. No differences in IL-10 levels were found throughout infection.
To the best of our knowledge, this is the first report addressing the role of NOX2 in experimental murine infection with L. amazonensis. Our results indicate that ROS might regulate the inflammation caused in L. amazonensis infection, but does not affect parasite control. The mechanisms used by L. amazonensis to avoid killing by ROS still need to be addressed.
EHR, GRG, JLG, DC and LQV are CNPq fellows. JPM was a CAPES fellow. This work was supported by grants from Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG, CDS-RED-00013-14, APQ-02308-13 and APQ-01419-14). The authors are members of the INCT de Processos Redox em Biomedicina-Redoxoma (FAPESP/CNPq/CAPES, proc 573530/2008-4). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Pace D. Leishmaniasis. J Infect. 2014;69 Suppl 1:S10–8.View ArticlePubMedGoogle Scholar
- Sadick MD, Locksley RM, Tubbs C, Raff HV. Murine cutaneous leishmaniasis: resistance correlates with the capacity to generate interferon-gamma in response to Leishmania antigens in vitro. J Immunol. 1986;136(2):655–61.PubMedGoogle Scholar
- Locksley RM, Heinzel FP, Sadick MD, Holaday BJ, Gardner Jr KD. Murine cutaneous leishmaniasis: susceptibility correlates with differential expansion of helper T-cell subsets. Ann Inst Pasteur Immunol. 1987;138(5):744–9.View ArticlePubMedGoogle Scholar
- Sadick MD, Heinzel FP, Shigekane VM, Fisher WL, Locksley RM. Cellular and humoral immunity to Leishmania major in genetically susceptible mice after in vivo depletion of L3T4+ T cells. J Immunol. 1987;139(4):1303–9.PubMedGoogle Scholar
- Anderson CF, Mendez S, Sacks DL. Nonhealing infection despite Th1 polarization produced by a strain of Leishmania major in C57BL/6 mice. J Immunol. 2005;174(5):2934–41.View ArticlePubMedGoogle Scholar
- Afonso LC, Scott P. Immune responses associated with susceptibility of C57BL/10 mice to Leishmania amazonensis. Infect Immun. 1993;61(7):2952–9.PubMedPubMed CentralGoogle Scholar
- Jones DE, Ackermann MR, Wille U, Hunter CA, Scott P. Early enhanced Th1 response after Leishmania amazonensis infection of C57BL/6 interleukin-10-deficient mice does not lead to resolution of infection. Infect Immun. 2002;70(4):2151–8.Google Scholar
- Soong L, Chang CH, Sun J, Longley Jr BJ, Ruddle NH, Flavell RA, McMahon-Pratt D. Role of CD4+ T cells in pathogenesis associated with Leishmania amazonensis infection. J Immunol. 1997;158(11):5374–83.PubMedGoogle Scholar
- Green SJ, Crawford RM, Hockmeyer JT, Meltzer MS, Nacy CA. Leishmania major amastigotes initiate the L-arginine-dependent killing mechanism in IFN-gamma-stimulated macrophages by induction of tumor necrosis factor-alpha. J Immunol. 1990;145(12):4290–7.PubMedGoogle Scholar
- Liew FY, Millott S, Parkinson C, Palmer RM, Moncada S. Macrophage killing of Leishmania parasite in vivo is mediated by nitric oxide from L-arginine. J Immunol. 1990;144(12):4794–7.PubMedGoogle Scholar
- Gomes IN, Calabrich AF, Tavares Rda S, Wietzerbin J, de Freitas LA, Veras PS. Differential properties of CBA/J mononuclear phagocytes recovered from an inflammatory site and probed with two different species of Leishmania. Microbes Infect. 2003;5(4):251–60.View ArticlePubMedGoogle Scholar
- Horta MF, Mendes BP, Roma EH, Noronha FS, Macedo JP, Oliveira LS, Duarte MM, Vieira LQ. Reactive oxygen species and nitric oxide in cutaneous leishmaniasis. J Parasitol Res. 2012;2012:203818.View ArticlePubMedPubMed CentralGoogle Scholar
- Rada B, Leto TL. Oxidative innate immune defenses by Nox/Duox family NADPH oxidases. Contrib Microbiol. 2008;15:164–87.View ArticlePubMedPubMed CentralGoogle Scholar
- Chan YC, Leung PS. The Renin-angiotensin system and reactive oxygen species: implications in pancreatitis. Antioxid Redox Signal. 2011;15(10):2743–55.View ArticlePubMedGoogle Scholar
- Landry WD, Cotter TG. ROS signalling, NADPH oxidases and cancer. Biochem Soc Trans. 2014;42(4):934–8.View ArticlePubMedGoogle Scholar
- Nusse O. Biochemistry of the phagosome: the challenge to study a transient organelle. ScientificWorldJournal. 2011;11:2364–81.View ArticlePubMedPubMed CentralGoogle Scholar
- Kavoosi G, Ardestani SK, Kariminia A. The involvement of TLR2 in cytokine and reactive oxygen species (ROS) production by PBMCs in response to Leishmania major phosphoglycans (PGs). Parasitology. 2009;136(10):1193–9.View ArticlePubMedGoogle Scholar
- Pawate S, Shen Q, Fan F, Bhat NR. Redox regulation of glial inflammatory response to lipopolysaccharide and interferongamma. J Neurosci Res. 2004;77(4):540–51.View ArticlePubMedGoogle Scholar
- Cachat J, Deffert C, Hugues S, Krause KH. Phagocyte NADPH oxidase and specific immunity. Clin Sci (Lond). 2015;128(10):635–48.View ArticleGoogle Scholar
- Babior BM. NADPH oxidase: an update. Blood. 1999;93(5):1464–76.PubMedGoogle Scholar
- Laroux FS, Romero X, Wetzler L, Engel P, Terhorst C. Cutting edge: MyD88 controls phagocyte NADPH oxidase function and killing of gram-negative bacteria. J Immunol. 2005;175(9):5596–600.View ArticlePubMedGoogle Scholar
- Shatwell KP, Segal AW. NADPH oxidase. Int J Biochem Cell Biol. 1996;28(11):1191–5.View ArticlePubMedGoogle Scholar
- Pollock JD, Williams DA, Gifford MA, Li LL, Du X, Fisherman J, Orkin SH, Doerschuk CM, Dinauer MC. Mouse model of X-linked chronic granulomatous disease, an inherited defect in phagocyte superoxide production. Nat Genet. 1995;9(2):202–9.View ArticlePubMedGoogle Scholar
- Segal AW. Absence of both cytochrome b-245 subunits from neutrophils in X-linked chronic granulomatous disease. Nature. 1987;326(6108):88–91.View ArticlePubMedGoogle Scholar
- Baehner RL, Nathan DG. Leukocyte oxidase: defective activity in chronic granulomatous disease. Science. 1967;155(3764):835–6.View ArticlePubMedGoogle Scholar
- Marriott HM, Jackson LE, Wilkinson TS, Simpson AJ, Mitchell TJ, Buttle DJ, Cross SS, Ince PG, Hellewell PG, Whyte MK et al. Reactive oxygen species regulate neutrophil recruitment and survival in pneumococcal pneumonia. Am J Respir Crit Care Med. 2008;177(8):887–95.View ArticlePubMedPubMed CentralGoogle Scholar
- Green SJ, Meltzer MS, Hibbs Jr JB, Nacy CA. Activated macrophages destroy intracellular Leishmania major amastigotes by an L-arginine-dependent killing mechanism. J Immunol. 1990;144(1):278–83.PubMedGoogle Scholar
- Liew FY, Li Y, Moss D, Parkinson C, Rogers MV, Moncada S. Resistance to Leishmania major infection correlates with the induction of nitric oxide synthase in murine macrophages. Eur J Immunol. 1991;21(12):3009–14.View ArticlePubMedGoogle Scholar
- Assreuy J, Cunha FQ, Epperlein M, Noronha-Dutra A, O’Donnell CA, Liew FY, Moncada S. Production of nitric oxide and superoxide by activated macrophages and killing of Leishmania major. Eur J Immunol. 1994;24(3):672–6.View ArticlePubMedGoogle Scholar
- Blos M, Schleicher U, Soares Rocha FJ, Meissner U, Rollinghoff M, Bogdan C. Organ-specific and stage-dependent control of Leishmania major infection by inducible nitric oxide synthase and phagocyte NADPH oxidase. Eur J Immunol. 2003;33(5):1224–34.View ArticlePubMedGoogle Scholar
- Sousa-Franco J, Araujo-Mendes E, Silva-Jardim I, L-Santos J, Faria DR, Dutra WO, Horta MF. Infection-induced respiratory burst in BALB/c macrophages kills Leishmania guyanensis amastigotes through apoptosis: possible involvement in resistance to cutaneous leishmaniasis. Microbes Infect. 2006;8(2):390–400.View ArticlePubMedGoogle Scholar
- Murray HW, Nathan CF. Macrophage microbicidal mechanisms in vivo: reactive nitrogen versus oxygen intermediates in the killing of intracellular visceral Leishmania donovani. J Exp Med. 1999;189(4):741–6.View ArticlePubMedPubMed CentralGoogle Scholar
- Almeida TF, Palma LC, Mendez LC, Noronha-Dutra AA, Veras PS. Leishmania amazonensis fails to induce the release of reactive oxygen intermediates by CBA macrophages. Parasite Immunol. 2012;34(10):492–8.View ArticlePubMedPubMed CentralGoogle Scholar
- Pham NK, Mouriz J, Kima PE. Leishmania pifanoi amastigotes avoid macrophage production of superoxide by inducing heme degradation. Infect Immun. 2005;73(12):8322–33.View ArticlePubMedPubMed CentralGoogle Scholar
- Mukbel RM, Patten Jr C, Gibson K, Ghosh M, Petersen C, Jones DE. Macrophage killing of Leishmania amazonensis amastigotes requires both nitric oxide and superoxide. Am J Trop Med Hyg. 2007;76(4):669–75.PubMedGoogle Scholar
- Oliveira MA, Tadokoro CE, Lima GM, Mosca T, Vieira LQ, Leenen PJ, Abrahamsohn IA. Macrophages at intermediate stage of maturation produce high levels of IL-12 p40 upon stimulation with Leishmania. Microbes Infect. 2005;7(2):213–23.View ArticlePubMedGoogle Scholar
- Spath GF, Beverley SM. A lipophosphoglycan-independent method for isolation of infective Leishmania metacyclic promastigotes by density gradient centrifugation. Exp Parasitol. 2001;99(2):97–103.View ArticlePubMedGoogle Scholar
- Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951;193(1):265–75.PubMedGoogle Scholar
- Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR. Analysis of nitrate, nitrite, and [15N] nitrate in biological fluids. Anal Biochem. 1982;126:131–8.View ArticlePubMedGoogle Scholar
- Allen RC, Loose LD. Phagocytic activation of a luminol-dependent chemiluminescence in rabbit alveolar and peritoneal macrophages. Biochem Biophys Res Commun. 1976;69(1):245–52.View ArticlePubMedGoogle Scholar
- Denicola A, Rubbo H, Rodriguez D, Radi R. Peroxynitrite-mediated cytotoxicity to Trypanosoma cruzi. Arch Biochem Biophys. 1993;304(1):279–86.View ArticlePubMedGoogle Scholar
- Cargnelutti DE, Salomon MC, Celedon V, Cuello-Carrion FD, Gea S, Di Genaro MS, Scodeller EA. Impact of tumor necrosis factor receptor p55 deficiency in susceptibility of C57BL/6 mice to infection with Leishmania (Leishmania) amazonensis. J Microbiol Immunol Infect. 2014;49(2):271-5.Google Scholar
- Felizardo TC, Gaspar-Elsas MI, Lima GM, Abrahamsohn IA. Lack of signaling by IL-4 or by IL-4/IL-13 has more attenuating effects on Leishmania amazonensis dorsal skin--than on footpad-infected mice. Exp Parasitol. 2012;130(1):48–57.View ArticlePubMedGoogle Scholar
- Carneiro MB, Lopes ME, Vaz LG, Sousa LM, Dos Santos LM, de Souza CC, Campos AC, Gomes DA, Goncalves R, Tafuri WL, et al. IFN-gamma-Dependent recruitment of CD4(+) T cells and macrophages contributes to pathogenesis during Leishmania amazonensis infection. J Interferon Cytokine Res. 2015;35(12):935–47.View ArticlePubMedGoogle Scholar
- Pinheiro RO, Rossi-Bergmann B. Interferon-gamma is required for the late but not early control of Leishmania amazonensis infection in C57Bl/6 mice. Mem Inst Oswaldo Cruz. 2007;102(1):79–82.View ArticlePubMedGoogle Scholar
- Gibson-Corley KN, Bockenstedt MM, Li H, Boggiatto PM, Phanse Y, Petersen CA, Bellaire BH, Jones DE. An in vitro model of antibody-enhanced killing of the intracellular parasite Leishmania amazonensis. PLoS One. 2014;9(9):e106426.View ArticlePubMedPubMed CentralGoogle Scholar
- Linares E, Giorgio S, Mortara RA, Santos CX, Yamada AT, Augusto O. Role of peroxynitrite in macrophage microbicidal mechanisms in vivo revealed by protein nitration and hydroxylation. Free Radic Biol Med. 2001;30(11):1234–42.View ArticlePubMedGoogle Scholar
- Giorgio S, Linares E, Ischiropoulos H, Von Zuben FJ, Yamada A, Augusto O. In vivo formation of electron paramagnetic resonance-detectable nitric oxide and of nitrotyrosine is not impaired during murine leishmaniasis. Infect Immun. 1998;66(2):807–14.PubMedPubMed CentralGoogle Scholar
- Lodge R, Diallo TO, Descoteaux A. Leishmania donovani lipophosphoglycan blocks NADPH oxidase assembly at the phagosome membrane. Cell Microbiol. 2006;8:1922–31.View ArticlePubMedGoogle Scholar
- Mosser DM, Edelson PJ. The mouse macrophage receptor for C3bi (CR3) is a major mechanism in the phagocytosis of Leishmania promastigotes. J Immunol. 1985;135:2785–9.PubMedGoogle Scholar
- Chang KP. Leishmania donovani-macrophage binding mediated by surface glycoproteins/antigens: characterization in vitro by a radioisotopic assay. Mol Biochem Parasitol. 1981;4:67–76.View ArticlePubMedGoogle Scholar
- Murray HW. Susceptibility of Leishmania to oxygen intermediates and killing by normal macrophages. J Exp Med. 1981;153:1302–15.View ArticlePubMedPubMed CentralGoogle Scholar
- Sousa LM, Carneiro MB, Resende ME, Martins LS, Dos Santos LM, Vaz LG, Mello PS, Mosser DM, Oliveira MA, Vieira LQ. Neutrophils have a protective role during early stages of Leishmania amazonensis infection in BALB/c mice. Parasite Immunol. 2014;36(1):13–31.View ArticlePubMedPubMed CentralGoogle Scholar
- Ottonello L, Frumento G, Arduino N, Bertolotto M, Dapino P, Mancini M, Dallegri F. Differential regulation of spontaneous and immune complex-induced neutrophil apoptosis by proinflammatory cytokines. Role of oxidants, Bax and caspase-3. J Leukoc Biol. 2002;72(1):125–32.PubMedGoogle Scholar
- Yamamoto A, Taniuchi S, Tsuji S, Hasui M, Kobayashi Y. Role of reactive oxygen species in neutrophil apoptosis following ingestion of heat-killed Staphylococcus aureus. Clin Exp Immunol. 2002;129(3):479–84.View ArticlePubMedPubMed CentralGoogle Scholar
- Kasahara Y, Iwai K, Yachie A, Ohta K, Konno A, Seki H, Miyawaki T, Taniguchi N. Involvement of reactive oxygen intermediates in spontaneous and CD95 (Fas/APO-1)-mediated apoptosis of neutrophils. Blood. 1997;89(5):1748–53.PubMedGoogle Scholar
- Gamberale R, Giordano M, Trevani AS, Andonegui G, Geffner JR. Modulation of human neutrophil apoptosis by immune complexes. J Immunol. 1998;161(7):3666–74.PubMedGoogle Scholar
- Hiraoka W, Vazquez N, Nieves-Neira W, Chanock SJ, Pommier Y. Role of oxygen radicals generated by NADPH oxidase in apoptosis induced in human leukemia cells. J Clin Invest. 1998;102(11):1961–8.View ArticlePubMedPubMed CentralGoogle Scholar
- Kobayashi SD, Voyich JM, Braughton KR, Whitney AR, Nauseef WM, Malech HL, DeLeo FR. Gene expression profiling provides insight into the pathophysiology of chronic granulomatous disease. J Immunol. 2004;172(1):636–43.View ArticlePubMedGoogle Scholar
- Coxon A, Rieu P, Barkalow FJ, Askari S, Sharpe AH, von Andrian UH, Arnaout MA, Mayadas TN. A novel role for the beta 2 integrin CD11b/CD18 in neutrophil apoptosis: a homeostatic mechanism in inflammation. Immunity. 1996;5(6):653–66.View ArticlePubMedGoogle Scholar
- van de Loo FA, Bennink MB, Arntz OJ, Smeets RL, Lubberts E, Joosten LA, Lent PL, Coenen-de Roo CJ, Cuzzocrea S, Segal BH, et al. Deficiency of NADPH oxidase components p47phox and gp91phox caused granulomatous synovitis and increased connective tissue destruction in experimental arthritis models. Am J Pathol. 2003;163(4):1525–37.Google Scholar
- Brown JR, Goldblatt D, Buddle J, Morton L, Thrasher AJ. Diminished production of anti-inflammatory mediators during neutrophil apoptosis and macrophage phagocytosis in chronic granulomatous disease (CGD). J Leukoc Biol. 2003;73(5):591–9.View ArticlePubMedGoogle Scholar
- Hampton MB, Fadeel B, Orrenius S. Redox regulation of the caspases during apoptosis. Ann N Y Acad Sci. 1998;854:328–35.View ArticlePubMedGoogle Scholar
- Hampton MB, Vissers MC, Keenan JI, Winterbourn CC. Oxidant-mediated phosphatidylserine exposure and macrophage uptake of activated neutrophils: possible impairment in chronic granulomatous disease. J Leukoc Biol. 2002;71(5):775–81.PubMedGoogle Scholar
- Schappi M, Deffert C, Fiette L, Gavazzi G, Herrmann F, Belli D, Krause KH. Branched fungal beta-glucan causes hyperinflammation and necrosis in phagocyte NADPH oxidase-deficient mice. J Pathol. 2008;214(4):434–44.View ArticlePubMedGoogle Scholar
- Geiszt M, Kapus A, Nemet K, Farkas L, Ligeti E. Regulation of capacitative Ca2+ influx in human neutrophil granulocytes. Alterations in chronic granulomatous disease. J Biol Chem. 1997;272(42):26471–8.View ArticlePubMedGoogle Scholar
- Rada BK, Geiszt M, Van Bruggen R, Nemet K, Roos D, Ligeti E. Calcium signalling is altered in myeloid cells with a deficiency in NADPH oxidase activity. Clin Exp Immunol. 2003;132(1):53–60.View ArticlePubMedPubMed CentralGoogle Scholar
- Bedard K, Krause KH. The NOX family of ROS-generating NADPH oxidases: physiology and pathophysiology. Physiol Rev. 2007;87(1):245–313.View ArticlePubMedGoogle Scholar
- Hatanaka E, Carvalho BT, Condino-Neto A, Campa A. Hyperresponsiveness of neutrophils from gp 91phox deficient patients to lipopolysaccharide and serum amyloid A. Immunol Lett. 2004;94(1–2):43–6.View ArticlePubMedGoogle Scholar
- Lekstrom-Himes JA, Kuhns DB, Alvord WG, Gallin JI. Inhibition of human neutrophil IL-8 production by hydrogen peroxide and dysregulation in chronic granulomatous disease. J Immunol. 2005;174(1):411–7.View ArticlePubMedGoogle Scholar
- Barsig J, Kusters S, Vogt K, Volk HD, Tiegs G, Wendel A. Lipopolysaccharide-induced interleukin-10 in mice: role of endogenous tumor necrosis factor-alpha. Eur J Immunol. 1995;25(10):2888–93.View ArticlePubMedGoogle Scholar
- Hoshino H, Laan M, Sjostrand M, Lotvall J, Skoogh BE, Linden A. Increased elastase and myeloperoxidase activity associated with neutrophil recruitment by IL-17 in airways in vivo. J Allergy Clin Immunol. 2000;105(1 Pt 1):143–9.View ArticlePubMedGoogle Scholar
- Witowski J, Pawlaczyk K, Breborowicz A, Scheuren A, Kuzlan-Pawlaczyk M, Wisniewska J, Polubinska A, Friess H, Gahl GM, Frei U, et al. IL-17 stimulates intraperitoneal neutrophil infiltration through the release of GRO alpha chemokine from mesothelial cells. J Immunol. 2000;165(10):5814–21.View ArticlePubMedGoogle Scholar
- Kelly MN, Kolls JK, Happel K, Schwartzman JD, Schwarzenberger P, Combe C, Moretto M, Khan IA. Interleukin-17/interleukin-17 receptor-mediated signaling is important for generation of an optimal polymorphonuclear response against Toxoplasma gondii infection. Infect Immun. 2005;73(1):617–21.View ArticlePubMedPubMed CentralGoogle Scholar
- Yu JJ, Ruddy MJ, Wong GC, Sfintescu C, Baker PJ, Smith JB, Evans RT, Gaffen SL. An essential role for IL-17 in preventing pathogen-initiated bone destruction: recruitment of neutrophils to inflamed bone requires IL-17 receptor-dependent signals. Blood. 2007;109(9):3794–802.View ArticlePubMedPubMed CentralGoogle Scholar
- Bonizzi G, Piette J, Merville MP, Bours V. Cell type-specific role for reactive oxygen species in nuclear factor-kappaB activation by interleukin-1. Biochem Pharmacol. 2000;59:7–11.View ArticlePubMedGoogle Scholar