Fig. 6

iNOS mRNA expression in footpads and nitrite production by infected thioglycollate-elicited macrophages. a Wild type (WT) and gp91^phox−/− mice were infected with 1 × 10⁶ metacyclic promastigote forms of L. amazonensis in the right hind footpad and followed for 16 weeks. iNOS mRNA levels were normalized by 18S mRNA expression 4, 8, 12 and 16 weeks post-infection. b Thioglycollate-elicited macrophages were harvested from the peritoneal cavity of WT or gp91^phox−/− mice 3 days after stimulation. Macrophages were infected with L. amazonensis metacyclic promastigotes (10 parasites per macrophage) for 4 h and cultures were washed. After 48 h of infection supernatants were collected and used to measure nitrite levels by Griess reaction. Data are shown as mean ± SD of one representative experiment of four, n = 5 for each experiment