Experimental transmission of Babesia microti by Rhipicephalus haemaphysaloides
© Li et al. 2016
Received: 27 January 2016
Accepted: 18 April 2016
Published: 25 April 2016
Human babesiosis is considered an emerging threat in China. Dozens of human infections with Babesia microti have been reported recently, especially in southern China. However, the transmission vectors of this parasite in these areas are not well understood. Rhipicephalus haemaphysaloides, which is one of the dominant tick species in southern China, is a major vector of bovine babesiosis in China. However, whether this tick has the potential to transmit B. microti has not been tested. The present study experimentally investigated the transmission competence of B. microti through R. haemaphysaloides ticks.
Larvae and nymphs of R. haemaphysaloides ticks were fed on laboratory mice infected by B. microti. The infection was detected by PCR at 4 weeks post-molting. BALB/c and NOD/SCID mice were infested by nymphs molting from larvae that ingested the blood of infective mice, and blood samples were then analyzed by PCR.
Experimental transstadial transmission of R. haemaphysaloides for B. microti was proved in both the larvae to nymph and the nymph to adult transstadial routes. The positive rate of B. microti was 43.8 % in nymphs developed from larvae consumed infected mice and 96.7 % in adults developed from nymphs exposed to positive mice. Among the mice infested by infective nymphs, B. microti was detected in 16.7 % (2/12) of the BALB/c mice and in all of the NOD/SCID (6/6). However, the parasite was not observed to persist beyond more than one molt, and transovarial transmission did not occur.
This is the first study to demonstrate that B. microti can be transmitted artificially by R. haemaphysaloides. This tick species might be a potential vector of human babesiosis in southern China, which represents a public health concern.
KeywordsBabesia microti Rhipicephalus haemaphysaloides Transmission Vector competence
Babesia microti is a tick-transmitted, intraerythrocytic parasite that usually infects wild animals worldwide. This parasite is also known to be one of the major causative agents of human babesiosis and endemic mainly in the northeastern and upper midwestern United States . Sporadic cases of B. microti or B. microti-like organisms have also been documented in places such as Germany, Japan, South Korea and Mongolia [1, 2]. In China, human cases of B. microti have been increasingly reported in recent years. In 2013, Zhou et al. reported 11 cases in Yunnan Province of southwestern China [3–5]. In 2015, Qiao et al. diagnosed a babesiosis patient and found 40 infected cases in colleagues of the patient in Guangxi Province of southern China . Moreover, most medical staff are unaware of the risk, and investigation of this risk in the mass population is scarce. Hence, the number of human infections with this parasite might be largely underestimated. Together with the fact that human infections by other babesia species have also increased significantly in China [7, 8], it is believed that babesiosis is an emerging threat in the country .
Ticks of the genus Ixodes are considered the primary vector of B. microti . The parasite is mainly transmitted to people by Ixodes scapularis in the United States. Ixodes spinipalpis is also a known vector, and still other Ixodes spp., including I. angustus, I. eastoni and I. muris, are suspected to transmit the parasite in North America. In many European countries, the primary vector of B. microti is usually I. ricinus, although I. trianguliceps is believed to play a more important role in England . Infections by I. persulcatus have also been reported in Russia. In Asia, B. microti Hobetsu type have been detected in questing I. ovatus from Japan, and US type and a type related to B. microti Kobe have been detected in I. persulcatus [10, 12–15]. There are few studies on the vectors of B. microti in China, except in the northeastern regions, where I. persulcatus is considered the primary vector [13, 16]. However, competent vectors of B. microti in other areas of the country are not yet well understood .
Interestingly, B. microti was recently detected in ticks outside the Ixodes genus. Wójcik-Fatla and colleagues detected infections in questing Dermacentor reticulatus ticks from Poland , and the parasite was reported in questing Haemaphysalis concinna ticks from northeast China . Therefore, more studies are needed on the transmission potentials of tick species from other genera.
Rhipicephalus haemaphysaloides, a three-host hard tick, is widely distributed in southern China and other countries in Southeast Asia [17, 19]. This species has been reported to be a major vector of bovine babesiosis in China  and can transmit the Kyasanur Forest disease virus . As far as we are aware, the transmission of B. microti through R. haemaphysaloides has not been reported. To understand whether R. haemaphysaloides could play a role in the spread of B. microti in China, we performed a series of experiments to study the transmission competence of B. microti through a R. haemaphysaloides strain maintained in our laboratory.
Experimental mice and ticks
Specific-pathogen-free (SPF) female BALB/c or NOD/SCID mice weighing 16 to 18 grams were purchased from Shanghai Laboratory Animal Center, Chinese Academy of Sciences. All animal experiments were performed according to the protocols approved by the Ethics Committee at the National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention based in Shanghai.
An engorged Rhipicephalus haemaphysaloides female was removed from a dog in a village of Tengchong County, Yunnan province, China, in 2014. The tick was then maintained in our laboratory in an incubator at 25 °C, with 85 % relative humidity and a 14/10 h light/dark photoperiod regimen. The larvae and nymphs of R. haemaphysaloides used in this study were the third generation of the colony.
Transmission experiments of Babesia microti
(i) Transmission of B. microti from mice to ticks
Approximately 600 larvae were applied equally by brush to 2 mice with B. microti parasitemia of 20–30 % three days after incubation. Each mouse was collared with a plastic cap with central drilling to prevent grooming. In the same way, 4 infected mice were infested with nymphs by applying 30 nymphs to each animal. Mice infested with ticks were maintained over trays with water in the bottom from which detached engorged ticks were harvested.
Twenty engorged larvae (10 from each mouse) and 12 engorged nymphs (3 from each mouse) were placed in 70 % alcohol for storage before being analyzed for infection by PCR. The engorged ticks remaining were maintained in 6 bottles at 25 °C, with 85 % relative humidity and a 14/10 h light/dark photoperiod regimen.
Twenty-eight days after the first molted tick was observed, 80 nymphs or 30 adults (15 females and 15 males) were analyzed for infection by PCR. The remaining ticks were used for the following experiments.
(ii) Transmission of B. microti to mice by infected nymphs
Twelve BALB/c mice or 6 NOD/SCID mice were each infested with 20 nymphs that were infected with B. microti as larvae. Approximately 50 μl tail blood of mice was collected for B. microti detection on every other day from day 7 to 30 after infestation. For each blood sample, a thin blood smear was made with 2 μl of blood, fixed with methanol, Giemsa-stained and examined using a microscope. Forty-five microliters of blood was used for DNA extraction, and PCR was performed to examine B. microti infection. Blood collection was stopped after the mice were proved to be infected by PCR. The engorged nymphs were used for the transstadial persistence experiment.
(iii) Transstadial persistence and transovarial transmission experiments
Forty engorged nymphs dropped from the uninfected BALB/c mice in the second experiment were stored for PCR analysis. The remaining ticks were maintained for molting as described above. Twenty-eight days after the first molted tick was observed, 80 adults harvested were analyzed by PCR to investigate transstadial persistence.
The infected adult ticks obtained in the first experiment were fed on the shaved ears of a laboratory rabbit. Ten engorged females and all of the fed males were stored for PCR. The other engorged females were maintained in bottles for oviposition and then stored for PCR analysis. Transovarial transmission was investigated by examining the resulting eggs and larvae by PCR.
DNA was extracted from both tick samples and mouse blood using a DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol. DNA was extracted in pools of approximately 50 eggs from both the infected females and the resulting larvae and was extracted individually for other ticks.
Nested PCR was used to test for infection using a set of primers highly specific for B. microti. Partial fragments of 18S rRNA gene were amplified using the primer sets Bab1A (5′- GTCTTAGTATAAGCTTTTATACAGCG-3′) and Bab4A (5′-GATAGGTCAGAAACTTGAATGATACATCG-3′) for the first round and Bab2A (5′-CAGTTATAGTTTATTTGATGTTCGTTTTAC-3′) and Bab3A (5′-CGGCAAAGCCATGCGATTCGCTAAT-3′) for the second round, as described by Zamoto et al. . Negative and positive controls were always used in each PCR reaction. One negative control was used in each row of a 96-well PCR plate or 8-tube strip to exclude contamination. PCR was performed in a C1000 Touch™ Thermal Cycler (Bio-Rad laboratories Incorporation, California, USA) starting with a pre-PCR heat step of 3 min at 95 °C, followed by 35 cycles of 94 °C for 40 s, 57 and 72 °C for 30 s, and ending at 72 °C for 5 min. Samples with 154-bp PCR products were recognized as B. microti - positive.
Transmission of B. microti to ticks by infected mice
Infection of R. haemaphysaloides by exposure to mice infected with B. microti
Tick stage applied
No. of engorged ticks analyzed
No. of positive engorged ticks (%)
No. of ticks analyzed 4 week post molting
No. of positive molted ticks (%)*
A total of 105 engorged nymphs were collected from infected mice (Fig. 2). All 12 of the engorged nymphs tested by PCR were positive (Table 1). Eighty-six adults were developed from the remaining nymphs. Thirty adult ticks (15 females and 15 males) were selected and analyzed for infection 4 weeks after molting. All 15 (100 %) females and 14 of the 15 (93.3 %) males were positive (P > 0.05).
Transmission of B. microti to mice by infected nymphs
Infection of mice with B. microti by infected nymphs
No. of mice applied
No. (%) of infected mice*
Prepatent period (days after infestation)
Transstadial persistence and transovarial transmission
Transstadial persistence of B. microti in R. haemaphysaloides ticks
Stage of infected ticks
No. of blood-feeding ticks analyzed
Positive blood-feeding ticks, n (%)
No. of ticks detected 4 week post molting/oviposition
Positive ticks post molting/oviposition, n (%)
Infective adult ticks were fed on a rabbit, and 27 engorged female ticks were collected (Fig. 2). Only 10 % (1/10) of the engorged females detected without oviposition were B. microti positive (Table 3). Two (11.8 %) of the 17 females were positive after oviposition. All the eggs and larvae were negative.
Dozens of human infections of B. microti have been reported recently, especially in southern China [6, 9]. However, the transmission vectors of this parasite in these areas are not well understood. Moreover, Rhipicephalus haemaphysaloides ticks are very common and widespread in southern China . Thus, investigating the transmission capability of R. haemaphysaloides for B. microti is needed.
As far as we are aware, this study is the first to investigate the transmission of B. microti using laboratory experiments in ticks of the genus Rhipicephalus. The present study confirmed the experimental transstadial transmission of R. haemaphysaloides for B. microti in two transstadial routes, i.e. larva to nymph (43.8 %) and nymph to adult (96.7 %). The transmission efficiency of R. haemaphysaloides from infected animals is comparable to that of Ixodes scapularis and I. ricinus, which are the primary vectors of B. microti in the United States and Europe [24, 25]. The results also showed that the transmission efficiency from nymph to adult was higher than that from larva to nymph (Table 1). This could be explained by nymphs ingesting much more infected blood than larvae do during feeding .
Consistent with other studies [24, 25], this study shows that the parasite of B. microti does not persist beyond more than one molt (Table 3) and that transovarial transmission does not occur. Interestingly, we also found that positive rate of tick infection declined dramatically after blood feeding (from 43.8 to 12.5 % for infected nymphs and from 100 to 10.0 % for female adults, as shown in Tables 1 and 3). The results suggest that blood-feeding induces immune response to pathogen in ticks such as other blood-sucking vectors [27, 28]. However, more investigations should be performed to understand the mechanisms underlying this phenomenon and to investigate the vector-parasite interaction in ticks.
The severity of babesiosis depends primarily on the immune status of the hosts . Severe B. microti illness is usually seen among immunocompromised patients. Similarly, the present study showed that B. microti infection was much easier to establish from infective ticks in immunodeficient NOD/SCID mice than in immunocompetent BABL/C mice.
There are several limitations in our study. First, we did not witness any parasites by blood smear evaluation because the monitoring of the parasite was stopped after the first positive PCR result. Furthermore, no tick salivary glands were tested for infection in our study. These weak points reduced the confidence that our study proved the ability of R. haemaphysaloides as a natural vector, as nested PCR is prone to contamination. Secondly, the transmission competence of R. haemaphysaloides might be challenged in natural environments due to the brief duration of patent infections in wild animals as opposed to the prolonged parasitaemias produced by repeated syringe passage into inbred laboratory mice . Thirdly, the transmission of B. microti from ticks to natural hosts of this parasite was not investigated, and their susceptibility may be quite different from laboratory animals . Finally, transovarial transmission is probably more likely to occur when engorging female ticks are exposed to infection, but this cannot occur in nature because R. haemaphysaloides adult females do not engorge on the small rodent reservoir hosts of B. microti. To further understand the role of R. haemaphysaloides in transmission of B. microti in nature, further investigations are needed on the infection of questing ticks and on the transmission to R. haemaphysaloides ticks by chronic infections.
In conclusion, our demonstration of R. haemaphysaloides transmission to mice suggests that this tick is a potential vector of human babesiosis in areas where it occurs and may be a matter of public health concern.
The research has been partially supported by the Special Fund for Health Research in the Public Interest China (No.201202019), the fourth round of Three-Year Public Health Action Plan (2015–2017, No. GWIV-29) and by the National Science & Technology Major Program (NO 2012ZX10004-220).
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Vannier E, Krause PJ. Human babesiosis. N Engl J Med. 2012;366:2397–407.View ArticlePubMedGoogle Scholar
- Hong SH, Anu D, Jeong YI, Abmed D, Cho SH, Lee WJ, Lee SE. Molecular detection and seroprevalence of Babesia microti among stock farmers in Khutul City, Selenge Province, Mongolia. Korean J Parasitol. 2014;52:443–7.View ArticlePubMedPubMed CentralGoogle Scholar
- Zhou X, Li SG, Chen SB, Wang JZ, Xu B, Zhou HJ, Ge HX, Chen JH, Hu W. Co-infections with Babesia microti and Plasmodium parasites along the China-Myanmar border. Infect Dis Poverty. 2013;2:24.View ArticlePubMedPubMed CentralGoogle Scholar
- Zhou X, Li S, Wang J, Huang J, Zhou H, Chen J, Zhou XN. Emergence of human babesiosis along the border of China with Myanmar: Detection by PCR and confirmation by sequencing. Emerg Microbes Infect. 2014;3:e55.View ArticlePubMedPubMed CentralGoogle Scholar
- Zhou X, Xia S, Huang J, Tambo E, Ge HX, Zhou XN. Human babesiosis, an emerging tick-borne disease in the People’s Republic of China. Parasit Vectors. 2014;7:509.PubMedPubMed CentralGoogle Scholar
- Yan Q, Heng P, Huaimin Z, Jizhou Y. Nest-PCR identification of one human infected of Babesia microti in Guangxi and investigation on his colleagues (Article in Chinese). Int J Med Parasitol Dis. 2015;42:152–5.Google Scholar
- Jiang J, Zheng Y, Jiang R, Li H, Huo Q, Jiang B, Sun Y, Jia N, Wang Y, Ma L, Liu H, Chu Y, Ni X, Liu K, Song Y, Yao N, Wang H, Sun T, Cao W. Epidemiological, clinical, and laboratory characteristics of 48 cases of “Babesia venatorum” infection in China: a descriptive study. Lancet Infect Dis. 2014.Google Scholar
- Sun Y, Li SG, Jiang JF, Wang X, Zhang Y, Wang H, Cao WC. Babesia venatorum infection in child, China. Emerg Infect Dis. 2014;20:896–7.View ArticlePubMedPubMed CentralGoogle Scholar
- Vannier E, Krause PJ. Babesiosis in China, an emerging threat. Lancet Infect Dis. 2015;15:137–9.View ArticlePubMedGoogle Scholar
- Yabsley MJ, Shock BC. Natural history of zoonotic Babesia: role of wildlife reservoirs. Int J Parasitol Parasites Wildl. 2013;2:18–31.View ArticlePubMedPubMed CentralGoogle Scholar
- Bown KJ, Lambin X, Telford GR, Ogden NH, Telfer S, Woldehiwet Z, Birtles RJ. Relative importance of Ixodes ricinus and Ixodes trianguliceps as vectors for Anaplasma phagocytophilum and Babesia microti in field vole (Microtus agrestis) populations. Appl Environ Microbiol. 2008;74:7118–25.View ArticlePubMedPubMed CentralGoogle Scholar
- Zamoto-Niikura A, Tsuji M, Qiang W, Nakao M, Hirata H, Ishihara C. Detection of two zoonotic Babesia microti lineages, the Hobetsu and U.S. lineages, in two sympatric tick species, Ixodes ovatus and Ixodes persulcatus, respectively, in Japan. Appl Environ Microb. 2012;78:3424–30.View ArticleGoogle Scholar
- Sun Y, Liu G, Yang L, Xu R, Cao W. Babesia microti-like rodent parasites isolated from Ixodes persulcatus (Acari: Ixodidae) in Heilongjiang Province, China. Vet Parasitol. 2008;156:333–9.View ArticlePubMedGoogle Scholar
- Saito-Ito A, Yano Y, Dantrakool A, Hashimoto T, Takada N. Survey of rodents and ticks in human babesiosis emergence area in Japan: first detection of Babesia microti-like parasites in Ixodes ovatus. J Clin Microbiol. 2004;42:2268–70.View ArticlePubMedPubMed CentralGoogle Scholar
- Rar VA, Epikhina TI, Livanova NN, Panov VV. Genetic diversity of Babesia in Ixodes persulcatus and small mammals from North Ural and West Siberia, Russia. Parasitology. 2011;138:175–82.View ArticlePubMedGoogle Scholar
- Fan D, Li M, Xu H, Hu M, Zhang J, Sun Y. The situation of mice and ticks infected by Babesia microti (Article in Chinese). Chin J Hyg Insect Equip. 2012;18:48–50.Google Scholar
- Chen Z, Yang X, Bu F, Yang X, Yang X, Liu J. Ticks (Acari: Ixodoidea: Argasidae, Ixodidae) of China. Exp Appl Acarol. 2010;51:393–404.View ArticlePubMedGoogle Scholar
- Wojcik-Fatla A, Bartosik K, Buczek A, Dutkiewicz J. Babesia microti in adult Dermacentor reticulatus ticks from eastern Poland. Vector Borne Zoonotic Dis. 2012;12:841–3.View ArticlePubMedGoogle Scholar
- Yu X, Gong H, Zhou Y, Zhang H, Cao J, Zhou J. Differential sialotranscriptomes of unfed and fed Rhipicephalus haemaphysaloides, with particular regard to differentially expressed genes of cysteine proteases. Parasit Vectors. 2015;8:597.View ArticlePubMedPubMed CentralGoogle Scholar
- Yin H, Lu W, Luo J. Babesiosis in China. Trop Anim Health Prod. 1997;29:11S–5.View ArticlePubMedGoogle Scholar
- Bhat HR, Naik SV, Ilkal MA, Banerjee K. Transmission of Kyasanur Forest disease virus by Rhipicephalus haemaphysaloides ticks. Acta Virol. 1978;22:241–4.PubMedGoogle Scholar
- Ruebush MJ, Hanson WL. Susceptibility of five strains of mice to Babesia microti of human origin. J Parasitol. 1979;65:430–3.View ArticlePubMedGoogle Scholar
- Zamoto A, Tsuji M, Wei Q, Cho SH, Shin EH, Kim TS, Leonova, GN, Hagiwara K, Asakawa M, Kariwa H, Takashima I, Ishihara C. Epizootiologic survey for Babesia microti among small wild mammals in northeastern Eurasia and a geographic diversity in the BETA-Tubulin gene sequences. J Vet Med Sci. 2004;66:785–92.View ArticlePubMedGoogle Scholar
- Oliveira MR, Kreier JP. Transmission of Babesia microti using various species of ticks as vectors. J Parasitol. 1979;65:816–7.View ArticlePubMedGoogle Scholar
- Gray J, von Stedingk LV, Gurtelschmid M, Granstrom M. Transmission studies of Babesia microti in Ixodes ricinus ticks and gerbils. J Clin Microbiol. 2002;40:1259–63.View ArticlePubMedPubMed CentralGoogle Scholar
- Randolph SE. Quantifying parameters in the transmission of Babesia microti by the tick Ixodes trianguliceps amongst voles (Clethrionomys glareolus). Parasitology. 1995;110(Pt 3):287–95.View ArticlePubMedGoogle Scholar
- Pakpour N, Akman-Anderson L, Vodovotz Y, Luckhart S. The effects of ingested mammalian blood factors on vector arthropod immunity and physiology. Microbes Infect. 2013;15:243–54.View ArticlePubMedPubMed CentralGoogle Scholar
- Kopacek P, Hajdusek O, Buresova V, Daffre S. Tick innate immunity. Adv Exp Med Biol. 2010;708:137–62.View ArticlePubMedGoogle Scholar
- Piesman J, Spielman A. Babesia microti: infectivity of parasites from ticks for hamsters and white-footed mice. Exp Parasitol. 1982;53:242–8.View ArticlePubMedGoogle Scholar