Dietary phytochemicals modulate skin gene expression profiles and result in reduced lice counts after experimental infection in Atlantic salmon
© Jodaa Holm et al. 2016
Received: 14 December 2015
Accepted: 26 April 2016
Published: 10 May 2016
The use of phytochemicals is a promising solution in biological control against salmon louse (Lepeophtheirus salmonis). Glucosinolates belong to a diverse group of compounds used as protection against herbivores by plants in the family Brassicaceae, while in vertebrates, ingested glucosinolates exert health-promoting effects due to their antioxidant and detoxifying properties as well as effects on cell proliferation and growth. The aim of this study was to investigate if Atlantic salmon fed two different doses of glucosinolate-enriched feeds would be protected against lice infection. The effects of feeding high dose of glucosinolates before the infection, and of high and low doses five weeks into the infection were studied.
Skin was screened by 15 k oligonucleotide microarray and qPCR.
A 25 % reduction (P < 0.05) in lice counts was obtained in the low dose group and a 17 % reduction in the high dose group compared to fish fed control feed. Microarray analysis revealed induction of over 50 interferon (IFN)-related genes prior to lice infection. Genes upregulated five weeks into the infection in glucosinolate-enriched dietary groups included Type 1 pro-inflammatory factors, antimicrobial and acute phase proteins, extracellular matrix remodeling proteases and iron homeostasis regulators. In contrast, genes involved in muscle contraction, lipid and glucose metabolism were found more highly expressed in the skin of infected control fish.
Atlantic salmon fed glucosinolates had a significantly lower number of sea lice at the end of the experimental challenge. Feeding glucosinolates coincided with increased expression of IFN-related genes, and higher expression profiles of Type 1 immune genes late into the infection. In addition, regulation of genes involved in the metabolism of iron, lipid and sugar suggested an interplay between metabolism of nutrients and mechanisms of resistance.
Sea lice infections constitute a major and global problem for salmonid aquaculture. Infection control relies primarily on chemical treatments whose repertoire is limited because of resistance to existing anti-parasitic compounds . In addition, stress to the fish caused by frequent delousing events is of particular concern . In order to apply the optimal treatment, resistance monitoring in lice populations, implemented through a nationwide surveillance program in Norway, could be helpful [1–4]. The amount of chemotherapeutants used against lice in Norway has surged over recent years; in 2008, 218 kg (kg of active substance, excluding hydrogen peroxide) were used, compared to 6,810 kg in 2012, 8,403 kg in 2013 and 12,812 kg in 2014 . Despite differences in the dosage of used chemotherapeutants and increase in the general production of Atlantic salmon, the rise in the number of treated salmon is likely the result of resistance development in sea lice [3, 6]. Finding alternative strategies for managing lice infections is therefore becoming increasingly more important.
Salmon lice (Lepeophtheirus salmonis) immunomodulate their hosts by secreting a complex cocktail of bioactive compounds [7–9]. Release of these secretory/excretory products depends on the host species, being highest in response to Atlantic salmon . This is in line with comparative studies showing that Atlantic salmon is among the most susceptible salmonid species [11–16]. Activation of the Type 1 immunity, similar to mammalian Type 1 (Th1 and Th17 responses), could play a role in the resistance of salmonids to lice infections [12, 13, 17, 18], especially during early stages of infection . Pro-inflammatory Type 1 responses (and to a lesser extent Type 2 responses) in skin were negatively correlated to the number of L. salmonis chalimus stages in Atlantic salmon . Skewing of immunity towards the Type 2 immunophenotype with a strong immunosuppressive component in Atlantic salmon (termed Th2-modified) likely contributes to susceptibility of Atlantic salmon , while Type 2 responses seem to have a more beneficial role in coho salmon during later stages of infection with L. salmonis .
There are several encouraging examples of the use of orally delivered microbial immunostimulants that promote protective immune responses [19–22]. The use of dietary plant-derived bioactives is also considered a promising approach. Plants in the family Brassicaceae contain secondary metabolites called glucosinolates (GLs) that protect against herbivory , bacterial and fungal disease agents [24–26]. When plant cells are destroyed by chewing or other mechanical processing, the enzyme myrosinase comes into contact with GLs and hydrolyses them into isothiocyanates (ITCs). These compounds act as insect deterrents but might also be toxic to invertebrates upon ingestion [25, 27]. Their strong pungent flavor  may also mask the host smell and obscure the host recognition and/or attachment process by sea lice. A range of olfactory receptors have been identified in both L. salmonis and Caligus rogercresseyi [29–31].
Studies in mammals have revealed that GLs-derived ITCs exert chemopreventive effects mainly attributed to induction of antioxidant and detoxification pathways (reviewed in [32, 33]). A majority of in vivo and in vitro studies report anti-inflammatory effects of ITCs in a range of pathological conditions, organs and cell lines, including tumor cells [32, 34]. However, pro-inflammatory type 1 responses have also been seen in murine skin after exposure to ITCs , suggesting an organ dependent regulation of immune responses by ITCs.
To date, GLs and their breakdown products have not been investigated as feed additives against aquatic parasites of Atlantic salmon. In this study, we hypothesised that dietary GLs would modulate skin immune and physiological responses, prior to and during lice infection, thus interfering with the attachment and establishment of L. salmonis.
The experimental facilities used in this study at Ewos Innovation, Dirdal, Norway, number 131 was approved by the Norwegian Animal Research Authority 02.02.2012 until 25.01.16. The experiments/procedures have been conducted in accordance with the laws and regulations controlling experiments/procedures in live animals in Norway, e.g. the Animal Welfare Act of 20th December 1974, No 73, chapter VI sections 20–22 and the Regulation on Animal Experimentation of 15th January 1996.
Fish trials, production of feeds and copepodids
All trials were performed at Ewos Innovation’s Test Facility in Dirdal, Norway from October to December 2012. Fish tanks used in all trials were 500 l circular flow-through tanks with an average temperature and salinity of 8.7 °C and 27.4 ppt, respectively.
The feeds used in this study were produced at the Ewos Innovation plant in Dirdal, Norway. The fish were fed with the control feed (C) OPAL (EWOS Opal, EWOS, Norway) and two experimental (anti-attachment) feeds that contained GLs. The low dose feed (LD) had 3.61 % and the high dose feed (HD) had 13.0 % of the GLs-containing ingredient originating from a plant of the family Brassicaceae, with the approximate GLs content of 7.3 μmol/g and 26.4 μmol/g in LD and HD, respectively (see Additional file 1: Table S2 for details of the dietary composition). All diets had a pellet size of 5.5 mm.
Six tanks of fish were used in a parallel feed study (Fig. 1b) to assess the effect of GLs feeding per se (without infection). After one month of feeding control feed (acclimation), three tanks of fish were fed high dose (HD) diet. The other three tanks continued on control feed. These groups of fish were named not-infected high dose (NI-HD) and not-infected control (NI-C), respectively. Sampling of skin tissue was performed after 17–18 days of feeding using the same protocol as for the infected fish; skin tissue of 9 fish from each group were sampled, and weights and length of 18 fish from each group were registered.
Salmon lice (L. salmonis) used in this trial were collected from Oltesvik (Norway) in March 2012. To provide a predictable supply of lice for future trials, this lice population was propagated and maintained on Atlantic salmon hosts kept in the L. salmonis cultivation system in the Sea Lice lab at the Dirdal facility, which provided stable supply of robust wild-type lice. Lice and host fish were held in 850 l circular flow-through tanks and egg strings from egg-bearing females were collected from anaesthetised salmon. The anesthetic used was Finquel (100 mg/l, Scan Aqua, Årnes, Norway). During an incubation period of 14 days (9 °C), the egg strings were allowed to hatch and reach the infective copepodid stage. The number of copepodids was counted in a zooplankton-counting chamber to calculate the density. At least four samples of 50 ml each were taken to improve the accuracy of estimation.
RNA extraction and cDNA synthesis
Total RNA extraction was done using the RNAeasy Mini Kit (Qiagen, Hilden, Germany) after adding Trizol (GIBCO, Life Technologies, Carlsbad, CA, USA) and homogenizing 50 mg of samples with 1.4 mm zirconium oxide beads (VWR, Oslo, Norway). After this, chloroform was added, samples were centrifuged and the RNA supernatant was subsequently subjected to RNA cleanup according to Qiagen protocol. The concentration of RNA was determined by spectrophotometry using NanoDrop ND1000 (Nanodrop Technologies, Wilmington, DE, USA) and stored at -80 °C until further use. The integrity of total RNA was determined using an Agilent 2100 Bioanalyzer, and only samples with RNA integrity number (RIN) of 8 or higher were accepted. Genomic DNA contamination was excluded by performing qPCR reactions using isolated RNA as templates together with primers for elongation factor-1α (EF1A).
Microarray hybridization and data processing
Five fish from each group (I-C, I-LD, I-HD and NI-C), and four fish from NI-HD were analysed by 15 k Atlantic salmon microarray (SIQ6); these individuals were a subset of fish analysed by qPCR. All samples were compared to pooled reference RNA that consisted of two fish from all groups, except for I-LD. The test samples labelled with Cy5 and pooled reference with Cy3 were competitively hybridised to array slides. All reagents and equipment used for microarray analyses were from Agilent Technologies; protocols were used according to the manufacturer. Labelling and amplification of RNA was performed on 100 ng total RNA using Two-colour Quick Amp Labelling kits. Gene Expression Hybridization Kit was used for the fragmentation. Hybridizations were performed in a rotation oven for 17 h at 65 °C with rotation speed of 10 rpm, followed by 1 min washing of arrays with Gene Expression Wash Buffer I at room temperature and Gene Expression Wash Buffer II at 37 °C. To achieve an overall intensity ratio close to 1 between Cy3 and Cy5 channels with minimal saturation, the slides were scanned immediately using GenePix Personal 4100A scanner (Molecular Devices, Sunnyvale, CA, USA) at 5 μm resolution and with manually adjusted laser power. For feature extraction of fluorescence intensity values and assessment of spot quality that followed spot-grid alignment, the GenePix pro software 6.0 was used. Subsequent to filtration of low quality spots flagged by the software, Lowess normalization of log2-expression ratios (ER) was performed. Differentially expressed genes (DEGs) were selected by comparison with the not-infected control (NI-C): log2-ER > 0.6 and P < 0.05 in at least one group were the criteria used. Fold values of log2-ERs (DEGs) were then calculated. Nofima’s bioinformatics system (STARS) was used for data analyses.
To validate the microarray data and screen other genes of interest (see Additional file 1: Table S1 for the full list), 9 fish from each group (I-C, I-LD, I-HD and NI-HD), in addition to NI-C group of fish were analysed by qPCR. For each sample, 1,800 ng of RNA was used to synthesize cDNA using the cDNA Affinity Script (Agilent Technologies, Matriks AS, Oslo, Norway) following the manufacturer’s protocol. Every reaction contained 1 μl of random primers and 2 μl of oligo DT primers. Each gene was run in duplicates by adding 4 μl of 1:10 diluted cDNA from each fish, 1 ul of each primer (10 μM concentration) (see sequences in Additional file 1: Table S1) and LightCycler 480 SYBR Green I Master mix (Roche) to a final volume of 12 μl in 96-well plates. Cycling conditions in LightCycler 480 instrument (Roche, Applied Science) were 5 min denaturation step at 95 °C, 40 cycles of denaturation (10 s at 95 °C), annealing (20 s at 60 °C) and extension (15 s at 72 °C), followed by melting curve analysis with measurements of the fluorescence performed in the temperature range between 65–97 °C. The maximum-second-derivative method (Roche diagnostics) was used to find the crossing point (Cp) value. The relative expression of target genes was calculated by using the ΔΔCt method. The reference gene EF1A was selected as it is one of the most well-established reference gene in studies of Atlantic salmon tissues in general  as well as in lice infected tissues [12, 17, 21, 38–40]. In this study, the mean Cp value in each group varied less than 0.5 cycle. One-way ANOVA with subsequent Tukey’s multiple comparisons test in the GraphPad Prism Software were executed between each of the 5 groups. Specificity and efficiency were confirmed by melting curve analysis and two-fold serial dilutions of cDNA for each primer pair in triplicates, respectively. PCR efficiency for all genes ranged from 1.8–2.
Fish performance and lice counts
Distribution of gender and life stages of lice found on Atlantic salmon in the infected control group (I-C), infected group fed low inclusion level of GLs (I-LD) and infected group fed high inclusion level of GLs (I-HD)
Preadult 1 (males)
Preadult 1 (females)
Preadult 2 (males)
Preadult 2 (females)
Examples of immune genes with differential expression in skin (microarray results)
Chemokines, cytokines and receptors
C-C motif chemokine 19-1
C-C motif chemokine 19-2
C-C motif chemokine 13
Leukocyte cell-derived chemotaxin 2-1
Small inducible cytokine A13
C-C chemokine receptor type 3
Interleukin-20 receptor alpha chain
TNFa induced metalloreductase STEAP4
Neutrophil cytosolic factor 1
Complement factor H1 protein
Antimicrobial peptide NK-lysin
C1q-like specific protein
Cathelicidin antimicrobial peptide 2
MMP 13 or Collagenase 3
Lectins and coreceptors
C type lectin receptor A
CD209 antigen-like protein E
Gene expression responses to GLs, lice and their combinations: microarray analyses
Activation of the acute phase response genes in skin included increased expression in NI-HD and I-HD of serum amyloid A, A5 and amyloid beta A4 compared to NI-C (Fig. 2d). On the other side, lowest expression of genes encoding proteins with diverse transport and scavenger functions (many of which are classified as negative acute phase plasma proteins) was found in I-LD (Fig. 2d). Most other immune genes were also induced, either by GLs, lice or both, when compared to NI-C (Table 2).
Majority of genes affected by GLs in infected groups showed dose-dependent responses: changes in I-HD were either equal to or greater than I-LD. However, several matrix metalloproteinase (MMP) genes, critical for extracellular remodeling during wound healing and inflammation  reached maximum expression levels in I-LD. Increased MMP13 expression has been linked to lice resistance in our previous study . In this study, C1q-like specific protein showed the overall greatest expression change (31.38-fold) in the best-protected I-LD group compared to NI-C. Natterin-like protein (NATTL) is a homologue of NATTL gene in T. nattereri that codes for a protein found in the venomous secretions of this fish species  and neutrophil cytosolic factor 1 were stimulated only by combinations of GLs and lice but at much lower levels. The upregulated immune genes included a number of inflammatory mediators. Two C-C motif chemokines 19 (CCL19), which responded to GLs might have a role in T cell proliferation and maturation of DCs that promote Th1 rather than Th2 responses . Similar profile was seen for several other chemokines and receptors (Table 2). A Th2 marker C-C chemokine receptor type 3  was more highly expressed in GLs groups, a similar trend was shown by granzyme A, an effector molecule of T cells. Furthermore, the small inducible cytokine A13 (CCL13) (2.65-fold induced) is a chemoattractant for a diverse group of immune cells . High (5-fold) induction of IL-20 receptor alpha chain precursor (4.95-fold) in NI-HD, followed by 1.38 fold in I-HD was noteworthy as this receptor transduces highly pro-inflammatory signals in mammalian skin . The leukocyte cell-derived chemotaxin 2 was upregulated by lice (I-C) and even more so in I-HD, and is associated with responses to lice  and lice resistance  in our previous studies.
Genes for diverse innate effectors were also activated. Golgi-residing metalloreductase STEAP family member 4, associated with iron metabolism and inflammation [48, 49] responded only to GLs, being most highly induced in I-LD, suggesting links between iron regulation, inflammation and resistance to lice. The antimicrobial peptides NK-lysin and cathelicidin antimicrobial peptide 2 that have been correlated to lice resistance in our previous studies [17, 18, 39] were stimulated by both diet and lice infection, and their combination, while RNase 1, a cell-cidal effector and complement regulatory factor H1 were upregulated only by lice infection (I-C). Several lectins and lectin co-receptors present on leucocytes were induced by GLs (p-selectin, CD83, CD209 and CD97) or by a combination of lice and GLs (mannose-specific lectin and C type lectin receptor A).
Examples of genes with differential expression in skin involved in apoptosis, stress responses, cytoskeleton and steroid and lipid metabolism (microarray data)
Apoptosis and stress
G0/G1 switch protein 2
60 kDa heat shock protein, mitochondrial
Heat shock protein 4
Glutathione peroxidase type 2
Keratin type I cytoskeletal 17
Type I keratin S8
Type II keratin E3
Steroid and lipid metabolism
Fatty acid-binding protein, adipocyte
Sex hormone-binding globulin beta
Differential expression was seen in multiple genes encoding intracellular fibrous structural proteins. Joint treatments (diet and lice infection) induced several genes involved in keratinization (Table 3). Keratin type I cytoskeletal 17 and type II keratin E3 are parts of the epithelial cytoskeleton, which provides mechanical resilience of epithelial cells and in addition can be involved in intracellular signaling . Many more genes were downregulated and several functional groups showed highly coordinated expression changes (Fig. 3a, b). Two clusters of co-expressed genes included myofiber proteins and enzymes of sugar metabolism (30 and 17 features, respectively); higher concentration of GLs produced stronger downregulation in both groups. Myofiber genes included mainly components of the myocontractile apparatus: myosin light and heavy chains, actin, troponins and titin (Fig. 3a, b, d). Similar though weaker changes were observed in lipid and steroid metabolism (Table 3). A number of genes with roles in tissue differentiation, formation of extracellular matrix (ECM) and wound healing, including multiple collagens, transcription factors forkhead box Q1 and kruppel-like factor 11a, receptor exostosin-like 2 [52, 53] and transforming growth factor-beta-induced protein ig-h3  were downregulated by both GLs and lice infection (Fig. 3c). The effect was slightly enhanced by the combination of lice and high dose of GLs.
The use of anti-attachment feeds promises to be a safe, easy to administer and cost-effective approach against sea lice. The achieved reduction in parasite numbers amounted to 25 % and could thus only be complementary to other control measures within the integrated pest management. Further work is needed to determine the optimal dosage and other possible effects that the bioactive compounds contained in anti-attachment feeds might exert on fish.
The goals of this study were to screen the transcriptomic response in skin after feeding Atlantic salmon diets enriched with GLs as well as to examine the impact of two inclusion doses of GLs (LD and HD) on the outcome of lice infection. One of the key findings in this study was the massive upregulation of a large group of genes involved in or associated with innate antiviral responses  in NI-HD (Fig. 2a-c) (the fish in this study showed no apparent signs of any viral disease). This is of note as the suppression of antiviral pathways by lice has been repeatedly reported [12, 16–19, 38, 40]. Innate anti-viral genes are co-regulated with interferons that play a key part in regulation of antiviral and antibacterial responses . Stimulation of mice with ITCs resulted in increased expression level of canonical Th1 markers IFNγ and T-bet in the ear tissue . Possible association between antiviral gene expression and reduced level of lice infection was shown in two of our previous studies. One addressed protection by sex steroid hormones, which conferred a 50 % reduction in lice counts  while in the other one, selective breeding based on 150 tested families resulted in a difference of around 36 % in lice counts between the top five extremely susceptible and resistant families included in the study . The observations from the current study fit a previously suggested hypothesis, which states that responses similar to mammalian Type 1 pro-inflammatory responses (Th1/Th17) play a positive role in protection against L. salmonis in Atlantic salmon [11, 12, 18, 40, 62]. Importantly, the induction of antiviral genes observed in NI-HD also remained higher in L. salmonis-challenged fish fed GLs (I-HD, I-LD) five weeks post-infection.
A recent comparative transcriptomic study of pink, chum and Atlantic salmon found downregulation of antiviral immune genes in both the resistant (pink) and susceptible species (chum), thus drawing attention to other protective mechanisms in Pacific salmonids, centered around iron metabolism  and possibly availability of other nutrients. Highly diverse iron sequestration mechanisms appear to play a crucial role in resistance of pink salmon to L. salmonis, but iron withdrawal strategy in response to lice was also reported in Atlantic salmon . This study suggested that modulation of this line of defense could be achieved by nutrition. In line with the proposal that sequestration of iron away from lice constitutes an aspect of protection, we observed increased expression levels of several genes coding for iron carrying and heme-binding proteins in groups exposed to GLs, including serotransferrin 1 and 2 in I-HD, and metalloreductase STEAP4 in I-LD (Fig. 2d, Table 2). In further support of this view, findings presented in a related paper (Stanko Skugor, personal communication) outlined the role of liver, muscle and distal kidney in iron sequestration in Atlantic salmon fed GLs-containing feeds.
qPCR analysis confirmed higher expression in I-LD and I-HD groups of several pro-inflammatory cytokines, chemokines and effectors, including the neutrophil attractant LECT2 and cytokines IFNγ and IL17A involved in Th1 and Th17-guided immune responses in mammals (Fig. 4a, b). Preconditioning naïve salmon skin by feeding GLs appears to oppose suppression and modulation of host immunity by lice, as the lowest expression level of Type 1 genes was found in I-C fish at the end of the challenge trial (Fig. 2c). We also wish to draw the attention to context-dependent fine-tuning of skin responses by GLs, exemplified by IL17A and MPO regulation (Fig. 4b, c). Downregulation was observed in the not-infected group (NI-HD) and in contrast, high expression was found in GLs supplemented groups post-infection (I-LD and I-HD). This context-dependent regulation (in absence vs in presence of infection) indicates that the preconditioning by GLs acts at a level other than the effector/mediator molecules MPO and IL17A (e.g. at the level of sensors or adaptors). Additional studies are needed to understand this in more detail.
While induction of anti-inflammatory mediators and ECM components (e.g. collagens) involved in strengthening of the physical skin barrier characterised I-C group, the best-protected group (I-LD) showed the opposite response: highest induction of extracellular MMPs involved in digestion of collagens and other ECM proteins (Table 2). Marked upregulation of MMPs have been observed around lice attachment sites in Atlantic salmon [11, 17, 18, 40], and importantly, even more so in the resistant pink salmon 48 h post-infection . Together with MMPs, a number of immune genes was most highly expressed in I-LD and I-HD groups. The group included cathelicidin antimicrobial peptide 2 (Table 2), previously implied in estrogen-mediated protection  and found to be responsive in stock bred for increased lice resistance , as well as two other genes coding for antimicrobial proteins, namely granzyme A and natterin-like protein. These findings suggest that host-interactions with or the skin microbiome per se could play a role in resistance to lice, which may open an exciting new field for future investigations. Serum amyloid is induced early in skin and spleen during L. salmonis infection in Atlantic salmon . The relevance of serum amyloids as candidates of protection in this study (Fig. 2d) is underscored by observations of suppressed serum amyloid A in skin of susceptible species, and activation in resistant species [11, 12, 16]. Immune mediators might direct some of the observed changes in the group of genes governing tissue turnover of nutrients, e.g. expression of fatty acid-binding protein (Table 3), which was 4-fold higher in control compared to other infected groups, is regulated by Th2 cytokines IL4 and 13 in humans .
Co-regulation of genes encoding myofiber proteins and multiple glycolytic enzymes is a hallmark of transcriptomic changes observed in our previous studies [18, 38, 40], suggesting that contractions in lice infected skin are fueled by glycolytic oxidation of sugars. Suppression of contractile activity in GLs-fed groups implied in this study could be associated with protection against lice. Downregulation of a number of genes encoding lipid metabolism and tissue differentiation regulators and ECM components (Table 3) in fish exposed to GLs also deserves attention, as it likely affected composition and consequently physicochemical properties of skin and mucus. Dietary GLs treatment could result in skin and mucus becoming less nutritious for lice. Such changes could also interfere with the host recognition by L. salmonis and the ensuing fast attachment of parasites to skin. Lipids present in fish mucus play a significant role in defining its viscosity  with obvious consequences for the lice attachment process. Moreover, it has been known for a long time in mammals that lipid sebum extracts contain volatile host odor components involved in the attraction of parasites ; thus, changes in the lipid content or lipid composition might have the potential to affect retention/formation of host kairomone semi-chemicals in Atlantic salmon mucus. To what extent this plays a role in early or late stages of sea lice infection remains unknown, and additional studies are needed to better understand the underlying mechanisms.
Finally, the present study suggested that GLs-mediated suppression of sex hormone-binding globulin beta, involved in regulation of sex hormone levels , may have increased the availability of steroid hormones in protected fish (Table 3). The sex steroid hormonal system in fish skin is important in wound healing in sea bream  and is associated with protection against lice in Atlantic salmon . Future feeding studies could explore the possibility to specifically promote beneficial expression of sex steroid hormones in skin while avoiding adverse hormonal effects in non-target tissues.
Feeding Atlantic salmon anti-attachment feeds containing GLs resulted in skewing of inflammatory responses towards Type 1 immunity, including gene expression programs centred on interferons in the skin prior to infection. Such dietary preconditioning seems beneficial upon encounter with the parasite as activation and maintenance of Type 1 immune genes coincided with the reduction in lice numbers. In addition, GLs-mediated gene expression changes implicated in the physicochemical properties of skin and mucus and metabolism of nutrients (iron, lipids and sugar) might interfere with the host recognition, attachment process and development of the parasite.
Microarray gene expression data files have been deposited to Gene Expression Omnibus (GSE79393).
This research has been funded by the Research Council Norway, SFI-Sea Lice Research Centre, grant number 20351.
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- Aaen SM, Helgesen KO, Bakke MJ, Kaur K, Horsberg TE. Drug resistance in sea lice: a threat to salmonid aquaculture. Trends Parasitol. 2015;31:72–81.View ArticlePubMedGoogle Scholar
- Igboeli OO, Burka JF, Fast MD. Lepeophtheirus salmonis: a persisting challenge for salmon aquaculture. Animal Frontiers. 2014;4:22–32.View ArticleGoogle Scholar
- Helgesen KO, Bravo S, Sevatdal S, Mendoza J, Horsberg TE. Deltamethrin resistance in the sea louse Caligus rogercresseyi (Boxhall and Bravo) in Chile: bioassay results and usage data for antiparasitic agents with references to Norwegian conditions. J Fish Dis. 2014;37:877–90.View ArticlePubMedGoogle Scholar
- Helgesen KO, Horsberg TE. Single-dose field bioassay for sensitivity testing in sea lice, Lepeophtheirus salmonis: development of a rapid diagnostic tool. J Fish Dis. 2013;36:261–72.View ArticlePubMedGoogle Scholar
- Forbruket av lakselusmidler er høyt og øker fortsatt. http://www.fhi.no/artikler/?id=114175 (2015). Accessed 06 April 2016.
- Bravo S, Silva MT, Monti G. Efficacy of emamectin benzoate in the control of Caligus rogercresseyi on farmed Atlantic salmon (Salmo salar L.) in Chile from 2006 to 2007. Aquaculture. 2012;364–365:61–6.View ArticleGoogle Scholar
- Fast MD, Ross NW, Craft CA, Locke SJ, MacKinnon SL, Johnson SC. Lepeophtheirus salmonis: characterization of prostaglandin E (2) in secretory products of the salmon louse by RP-HPLC and mass spectrometry. Exp Parasitol. 2004;107:5–13.View ArticlePubMedGoogle Scholar
- Firth KJ, Johnson SC, Ross NW. Characterization of proteases in the skin mucus of Atlantic salmon (Salmo salar) infected with the salmon louse (Lepeophtheirus salmonis) and in whole-body louse homogenate. J Parasitol. 2000;86:1199–205.View ArticlePubMedGoogle Scholar
- McCarthy E, Cunningham E, Copley L, Jackson D, Johnston D, Dalton JP, Mulcahy G. Cathepsin L proteases of the parasitic copepod, Lepeophtheirus salmonis. Aquaculture. 2012;356–357:264–71.View ArticleGoogle Scholar
- Fast MD, Burka JF, Johnson SC, Ross NW. Enzymes released from Lepeophtheirus salmonis in response to mucus from different salmonids. J Parasitol. 2003;89:7–13.View ArticlePubMedGoogle Scholar
- Braden LM, Barker DE, Koop BF, Jones SR. Comparative defense-associated responses in salmon skin elicited by the ectoparasite Lepeophtheirus salmonis. Comp Biochem Physiol. 2012;7:100–9.Google Scholar
- Braden LM, Koop BF, Jones SRM. Signatures of resistance to Lepeophtheirus salmonis include a TH2-type response at the louse-salmon interface. Dev Comp Immunol. 2015;48:178–91.View ArticlePubMedGoogle Scholar
- Fast MD. Fish immune responses to parasitic copepod (namely sea lice) infection. Dev Comp Immunol. 2014;43:300–12.View ArticlePubMedGoogle Scholar
- Fast MD, Sims DE, Burka JF, Mustafa A, Ross NW. Skin morphology and humoral non-specific defence parameters of mucus and plasma in rainbow trout, coho and Atlantic salmon. Comp Biochem Phys A. 2002;132:645–57.View ArticleGoogle Scholar
- Johnson SC, Albright LJ. Comparative susceptibility and histopathology of the response of naive Atlantic, Chinook and Coho salmon to experimental-infection with Lepeophtheirus salmonis (Copepoda, Caligidae). Dis Aquat Org. 1992;14:179–93.Google Scholar
- Sutherland BJG, Koczka KW, Yasuike M, Jantzen SG, Yazawa R, Koop BF, Jones SRM. Comparative transcriptomics of Atlantic Salmo salar, chum Oncorhynchus keta and pink salmon O. gorbuscha during infections with salmon lice Lepeophtheirus salmonis. BMC Genomics. 2014;15:200.View ArticlePubMedPubMed CentralGoogle Scholar
- Holm H, Santi N, Kjøglum S, Perisic N, Skugor S, Evensen O. Difference in skin immune responses to infection with salmon louse (Lepeophtheirus salmonis) in Atlantic salmon (Salmo salar L.) of families selected for resistance and susceptibility. Fish Shellfish Immunol. 2015;42:384–94.View ArticlePubMedGoogle Scholar
- Skugor S, Glover KA, Nilsen F, Krasnov A. Local and systemic gene expression responses of Atlantic salmon (Salmo salar L.) to infection with the salmon louse (Lepeophtheirus salmonis). BMC Genomics. 2008;9:498.View ArticlePubMedPubMed CentralGoogle Scholar
- Covello JM, Friend SE, Purcell SL, Burka JF, Markham RJF, Donkin AW, Groman DB, Fast MD. Effects of orally administered immunostimulants on inflammatory gene expression and sea lice (Lepeophtheirus salmonis) burdens on Atlantic salmon (Salmo salar). Aquaculture. 2012;366:9–16.View ArticleGoogle Scholar
- Poley J, Purcell SL, Igboeli OO, Donkin A, Wotton H, Fast MD. Combinatorial effects of administration of immunostimulatory compounds in feed and follow-up administration of triple-dose SLICE® (emamectin benzoate) on Atlantic salmon, Salmo salar L., infection with Lepeophtheirus salmonis. J Fish Dis. 2013;36:299–309.View ArticlePubMedGoogle Scholar
- Purcell SL, Friend SE, Covello JM, Donkin A, Groman DB, Poley J, Fast MD. CpG inclusion in feed reduces sea lice, Lepeophtheirus salmonis, numbers following re-infection. J Fish Dis. 2013;36:229–40.View ArticlePubMedGoogle Scholar
- Refstie S, Baeverfjord G, Seim RR, Elvebø O. Effects of dietary yeast cell wall β-glucans and MOS on performance, gut health, and salmon lice resistance in Atlantic salmon (Salmo salar) fed sunflower and soybean meal. Aquaculture. 2010;305:109–16.View ArticleGoogle Scholar
- Tripathi MK, Mishra AS. Glucosinolates in animal nutrition: A review. Anim feed sci tech. 2007;132:1–27.View ArticleGoogle Scholar
- Aires A, Mota VR, Saavedra MJ, Rosa EA, Bennett RN. The antimicrobial effects of glucosinolates and their respective enzymatic hydrolysis products on bacteria isolated from the human intestinal tract. J Appl Microbiol. 2009;106:2086–95.View ArticlePubMedGoogle Scholar
- Bednarek P. Chemical warfare or modulators of defence responses – the function of secondary metabolites in plant immunity. Curr Opin Plant Biol. 2012;15:407–14.View ArticlePubMedGoogle Scholar
- Saavedra MJ, Dias CS, Martinez-Murcia A, Bennett RN, Aires A, Rosa EA. Antibacterial effects of glucosinolate-derived hydrolysis products against Enterobacteriaceae and enterococci isolated from pig ileum segments. Foodborne Pathog Dis. 2012;9:338–45.Google Scholar
- Textor S, Gershenzon J. Herbivore induction of the glucosinolate-myrosinase defense system: major trends, biochemical bases and ecological significance. Phytochem Rev. 2009;8:149–70.View ArticleGoogle Scholar
- Bruce TJ, Wadhams LJ, Woodcock CM. Insect host location: a volatile situation. Trends Plant Sci. 2005;10:269–74.View ArticlePubMedGoogle Scholar
- Mordue Luntz AJ, Birkett MA. A review of host finding behaviour in the parasitic sea louse, Lepeophtheirus salmonis (Caligidae: Copepoda). J Fish Dis. 2009;32:3–13.View ArticlePubMedGoogle Scholar
- Nuñez-Acuna G, Valenzuela-Muñoz V, Marambio JP, Wadsworth S, Gallardo-Escárate C. Insights into the olfactory system of the ectoparasite Caligus rogercresseyi: molecular characterization and gene transcription analysis of novel ionotropic receptors. Exp Parasitol. 2014;145:99–109.View ArticlePubMedGoogle Scholar
- Pino-Marambio J, Mordue AJ, Birkett M, Carvajal J, Asencio G, Mellado A, Quiroz A. Behavioural studies of host, non-host and mate location by the Sea Louse, Caligus rogercresseyi Boxshall & Bravo, 2000 (Copepoda : Caligidae). Aquaculture. 2007;271:70–6.View ArticleGoogle Scholar
- Fimognari C, Turrini E, Ferruzzi L, Lenzi M, Hrelia P. Natural isothiocyanates: genotoxic potential versus chemoprevention. Mutat Res. 2012;750:107–31.View ArticlePubMedGoogle Scholar
- Vig AP, Rampal G, Thind TS, Arora S. Bio-protective effects of glucosinolates - A review. LWT-Food Sci Technol. 2009;42:1561–72.View ArticleGoogle Scholar
- Dinkova-Kostova AT, Kostov RV. Glucosinolates and isothiocyanates in health and disease. Trends Mol Med. 2012;18:337–47.View ArticlePubMedGoogle Scholar
- Kim HJ, Barajas B, Wang M, Nel AE. Nrf2 activation by sulforaphane restores the age-related decrease of TH1 immunity: Role of dendritic cells. J Allergy Clin Immunol. 2008;121:1255–61.View ArticlePubMedPubMed CentralGoogle Scholar
- Haugland M, Holst JC, Holm M, Hansen LP. Feeding of Atlantic salmon (Salmo salar L.) post-smolts in the Northeast Atlantic. ICES J Mar Sci. 2006;63:1488–500.View ArticleGoogle Scholar
- Olsvik PA, Lie KK, Jordal A-EO, Nilsen TO, Hordvik I. Evaluation of potential reference genes in real-time RT-PCR studies of Atlantic salmon. BMC Mol Biol. 2005;6:1–9.View ArticleGoogle Scholar
- Krasnov A, Skugor S, Todorcevic M, Glover KA, Nilsen F. Gene expression in Atlantic salmon skin in response to infection with the parasitic copepod Lepeophtheirus salmonis, cortisol implant, and their combination. BMC Genomics. 2012;13:130.View ArticlePubMedPubMed CentralGoogle Scholar
- Krasnov A, Wesmajervi Breiland MS, Hatlen B, Afanasyev S, Skugor S. Sexual maturation and administration of 17β-estradiol and testosterone induce complex gene expression changes in skin and increase resistance of Atlantic salmon to ectoparasite salmon louse. Gen Comp Endocrinol. 2015;212C:34–43.View ArticleGoogle Scholar
- Tadiso TM, Krasnov A, Skugor S, Afanasyev S, Hordvik I, Nilsen F. Gene expression analyses of immune responses in Atlantic salmon during early stages of infection by salmon louse (Lepeophtheirus salmonis) revealed bi-phasic responses coinciding with the copepod-chalimus transition. BMC Genomics. 2011;12:141.View ArticlePubMedPubMed CentralGoogle Scholar
- Krasnov A, Timmerhaus G, Schiotz BL, Torgersen J, Afanasyev S, Iliev D, Jorgensen J, Takle H, Jorgensen SM. Genomic survey of early responses to viruses in Atlantic salmon. Salmo salar L. Mol Immunol. 2011;49:163–74.Google Scholar
- Visse R, Nagase H. Matrix metalloproteinases and tissue inhibitors of metalloproteinases: Structure, function, and biochemistry. Circ Res. 2003;92:827–39.View ArticlePubMedGoogle Scholar
- Magalhães GS, Lopes-Ferreira M, Junqueira-de-Azevedo ILM, Spencer PJ, Araújo MS, Portaro FCV, Ma L, Valente RH, Juliano L, Fox JW et al. Natterins, a new class of proteins with kininogenase activity characterized from Thalassophryne nattereri fish venom. Biochimie. 2005;87:687–99.View ArticlePubMedGoogle Scholar
- Marsland BJ, Battig P, Bauer M, Ruedl C, Lassing U, Beerli RR, Dietmeier K, Ivanova L, Pfister T, Vogt L et al. CCL19 and CCL21 induce a potent proinflammatory differentiation program in licensed dendritic cells. Immunity. 2005;22:493–505.View ArticlePubMedGoogle Scholar
- Sallusto F, Mackay CR, Lanzavecchia A. Selective expression of the eotaxin receptor CCR3 by human T helper 2 cells. Science. 1997;277:2005–7.View ArticlePubMedGoogle Scholar
- Mendez-Enriquez E, Garcia-Zepeda EA. The multiple faces of CCL13 in immunity and inflammation. Inflammopharmacology. 2013;21:397–406.View ArticlePubMedGoogle Scholar
- Blumberg H, Conklin D, Xu W, Grossmann A, Brender T, Carollo S, Eagan M, Foster D, Haldeman BA, Hammond A et al. Interleukin 20: Discovery, receptor identification, and role in epidermal function. Cell. 2001;104:9–19.View ArticlePubMedGoogle Scholar
- Gomes IM, Maia CJ, Santos CR. STEAP proteins: from structure to applications in cancer therapy. Mol Cancer Res. 2012;10:573–87.View ArticlePubMedGoogle Scholar
- Grunewald TG, Bach H, Cossarizza A, Matsumoto I. The STEAP protein family: versatile oxidoreductases and targets for cancer immunotherapy with overlapping and distinct cellular functions. Biol Cell. 2012;104:641–57.View ArticlePubMedGoogle Scholar
- Welch C, Santra MK, El-Assaad W, Zhu X, Huber WE, Keys RA, Teodoro JG, Green MR. Identification of a protein, G0S2, that lacks Bcl-2 homology domains and interacts with and antagonizes Bcl-2. Cancer Res. 2009;69:6782–9.View ArticlePubMedPubMed CentralGoogle Scholar
- Moll R, Divo M, Langbein L. The human keratins: biology and pathology. Histochem Cell Biol. 2008;129:705–33.View ArticlePubMedPubMed CentralGoogle Scholar
- Feuerborn A, Srivastava PK, Küffer S, Grandy WA, Sijmonsma TP, Gretz N, Brors B, Gröne HJ. The Forkhead factor FoxQ1 influences epithelial differentiation. J Cel Physiol. 2011;226:710–9.View ArticleGoogle Scholar
- Lai Y, Li D, Li C, Muehleisen B, Radek KA, Park HJ, Jiang Z, Li Z, Lei H, Quan Y et al. The antimicrobial protein REG3A regulates keratinocyte proliferation and differentiation after skin injury. Immunity. 2012;37:74–84.View ArticlePubMedGoogle Scholar
- Bae JS, Lee SH, Kim JE, Choi JY, Park RW, Yong PJ, Park HS, Sohn YS, Lee DS, Bae LE et al. Betaig-h3 supports keratinocyte adhesion, migration, and proliferation through alpha3beta1 integrin. Biochem Biophys Res Commun. 2002;294:940–8.View ArticlePubMedGoogle Scholar
- Lovoll M, Johnsen H, Boshra H, Bogwald J, Sunyer JO, Dalmo RA. The ontogeny and extrahepatic expression of complement factor C3 in Atlantic salmon (Salmo salar). Fish Shellfish Immunol. 2007;23:542–52.View ArticlePubMedGoogle Scholar
- Baggiolini M, Walz A, Kunkel SL. Neutrophil-activating peptide-1/interleukin 8, a novel cytokine that activates neutrophils. J Clin Invest. 1989;84:1045–9.View ArticlePubMedPubMed CentralGoogle Scholar
- Klebanoff SJ, Kettle AJ, Rosen H, Winterbourn CC, Nauseef WM. Myeloperoxidase: a front-line defender against phagocytosed microorganisms. J Leukoc Biol. 2013;93:185–98.View ArticlePubMedPubMed CentralGoogle Scholar
- Aggarwal S, Gurney AL. IL-17: prototype member of an emerging cytokine family. J Leukoc Biol. 2002;71:1–8.PubMedGoogle Scholar
- Martinez GJ, Nurieva RI, Yang XO, Dong C. Regulation and function of proinflammatory TH17 cells. Ann N Y Acad Sci. 2008;1143:188–211.View ArticlePubMedGoogle Scholar
- Takizawa F, Koppang EO, Ohtani M, Nakanishi T, Hashimoto K, Fischer U, Dijkstra JM. Constitutive high expression of interleukin-4/13A and GATA-3 in gill and skin of salmonid fishes suggests that these tissues form Th2-skewed immune environments. Mol Immunol. 2011;48:1360–8.View ArticlePubMedGoogle Scholar
- Robertsen B. The interferon system of teleost fish. Fish Shellfish Immunol. 2006;20:172–91.View ArticlePubMedGoogle Scholar
- Sutherland BJ, Jantzen SG, Sanderson DS, Koop BF, Jones SR. Differentiating size-dependent responses of juvenile pink salmon (Oncorhynchus gorbuscha) to sea lice (Lepeophtheirus salmonis) infections. Comp Biochem Physiol Part D Genomics Proteomics. 2011;6:213–23.View ArticlePubMedGoogle Scholar
- Shum BO, Mackay CR, Gorgun CZ, Frost MJ, Kumar RK, Hotamisligil GS, Rolph MS. The adipocyte fatty acid-binding protein aP2 is required in allergic airway inflammation. J Clin Invest. 2006;116:2183–92.View ArticlePubMedPubMed CentralGoogle Scholar
- Esteban MA. An overview of the immunological defenses in fish skin. ISRN Immunology. 2012;1–29.Google Scholar
- Osterkamp J, Wahl U, Schmalfuss G, Haas W. Host-odour recognition in two tick species is coded in a blend of vertebrate volatiles. J Comp Physiol A Neuroethol Sens Neural Behav Physiol. 1999;185:59–67.View ArticleGoogle Scholar
- Marivin E, Yano A, Guérin A, Nguyen TV, Fostier A, Bobe J, Guiguen Y. Sex hormone-binding globulins characterization and gonadal gene expression during sex differentiation in the rainbow trout, Oncorhynchus mykiss. Mol Reprod Dev. 2014;81:757–65.PubMedGoogle Scholar
- Ibarz A, Pinto PIS, Power DM. Proteomic Approach to skin regeneration in a marine teleost: modulation by Oestradiol-17 beta. Mar Biotechnol. 2013;15:629–46.View ArticlePubMedGoogle Scholar