Exposure to vector-borne pathogens in candidate blood donor and free-roaming dogs of northeast Italy

Parasites & Vectors

Table 1 Target genes and rPCR primers used in this study for pathogen identification

Target organism	Gene	Primer	Sequence (5'-3')	Pc (μM)	TA (°C)	Reference
Babesia/Theileria	18S rRNA	BJ1	GTCTTGTAATTGGAATGATGG	0.1	60	[31]
		BN2	TAGTTTATGGTTAGGACTACG	0.1
Rickettsia spp.	rompB	RompB OFm	GTAACCGGAARTAATCGTTTCGT	0.1	58	[32]
		RompB ORm	GCTTTATAACCAGCTAAACCRCC	0.1
Leishmania spp.	COII	COII F	GGCATAAATCCATGTAAGA	0.3	52	[33]
		COII R	TGGCTTTTATATTATCATTTT	0.3
Ehrlichia canis	16S rRNA	CANIS	CAATTATTTATAGCCTCTGGCTATAGGA	0.5	60	[34]
		CA1UR	GAGTTTGCCGGGACTTCTTCT	0.5

Abbreviations: TA temperature of annealing; Pc primer concentration
16S rRNA = gene coding 16S ribosomal RNA; rompB = Rickettsial Outer Membrane Protein B gene; COII = Cytochrome c oxidase subunit II gene ; 18S rRNA = gene coding 18S ribosomal RNA

ISSN: 1756-3305